ABSTRACT
Triphenyltin (TPT) is widely used in crop pest control and ship antifouling coatings, which leads to its entry into aquatic environment and poses a threat to aquatic organisms. However, the effects of TPT on the early life stages of wild fish in natural water environments remains unclear. The aim of this study was to assess the toxic effects of TPT on the early life stages of fish under two different environments: field investigation and laboratory experiment. The occurrence of deformities in wild fish embryos and larvae in the Three Gorges Reservoir (TGR) and the developmental toxicity of TPT at different concentrations (0, 0.15, 1.5 and 15 µg Sn/L) to zebrafish embryos and larvae were observed. The results showed that TPT content was higher in wild larvae, reaching 27.21 ng Sn/g w, and the malformation of wild fish larvae mainly occurred in the eyes and spine under natural water environment. Controlled experiment exposure of zebrafish larvae to TPT also resulted in eye and spinal deformities. Gene expression analysis showed that compared with the control group, the expression levels of genes related to eye development (sox2, otx2, stra6 and rx1) and spine development (sox9a and bmp2b) were significantly up-regulated in the 15 µg Sn/L exposure group, which may be the main cause of eye and spine deformity in the early development stage of fish. In addition, the molecular docking results further elucidate that the strong hydrophobic and electrostatic interactions between TPT and protein residues are the main mechanism of TPT induced abnormal gene expression. Based on these results, it can be inferred that TPT is one of the teratogenic factors of abnormal eye and spine development in the early life stage of fish in the TGR. These findings have important implications for understanding the toxicity of TPT on fish.
Subject(s)
Embryo, Nonmammalian , Larva , Organotin Compounds , Water Pollutants, Chemical , Zebrafish , Animals , Organotin Compounds/toxicity , Water Pollutants, Chemical/toxicity , Larva/drug effects , Embryo, Nonmammalian/drug effects , Spine/drug effects , Spine/abnormalities , Eye/drug effects , Eye/embryologyABSTRACT
Triphenyltin (TPhT) and tributyltin (TBT) remain widely present in various aquatic environments despite restrictions on their use in many countries for many years. The biomagnification of these compounds in the aquatic food web remains controversial. This study reports the bioaccumulation of TPhT and TBT in aquatic animals in the Three Gorges Reservoir (TGR), a deep-water river channel-type reservoir and the largest reservoir in China. We measured TPhT, TBT and their metabolites in 2 invertebrates, 27 fish and the aquatic environment. The logarithmic bioaccumulation factors of TPhT and TBT were 4.37 and 3.77, respectively, indicating that TPhT and TBT were enriched in organisms of the TGR. Both TPhT and TBT concentrations were significantly and positively correlated with trophic level, with trophic magnification factors of 3.71 and 3.63, respectively, indicating that TPhT and TBT exhibited similar trophic enrichment in the freshwater food web of the TGR. The results of health risk assessment showed that although all hazard index (HI) values were <1, more attention should be paid to the health risk to children associated with consumption of aquatic products (HIâ¯=â¯0.67). This study provides powerful evidence of trophic enrichment of TPhT and TBT in a freshwater food web in a deep-water river channel-type reservoir and provides valuable data regarding organotins in aquatic animals in the TGR.
Subject(s)
Organotin Compounds , Water Pollutants, Chemical , Animals , China , Environmental Monitoring/methods , Food Chain , Rivers , Water , Water Pollutants, Chemical/analysisABSTRACT
A novel bipolar NIR iridium(iii) complex (CH3OTPA-BTz-Iq)2Ir(pic-OXD) with both a hole transporting (HT) triphenylamine (TPA) group and an electron transporting (ET) oxadiazole (OXD) group was designed and synthesized. It was observed that the incorporation of OXD and TPA into the ligand (CH3OTPA-BTz-Iq)2Ir(pic-OXD) improved the optophysical and electroluminescence performance in comparison with the parent iridium(iii) complex (CH3OTPA-BTz-Iq)2Irpic. In (CH3OTPA-BTz-Iq)2Ir(pic-OXD)-based OLEDs, a maximum external quantum efficiency (EQEmax) of 1.15% at 716 nm was obtained, which is much superior than that of the (CH3OTPA-BTz-Iq)2Irpic-based OLEDs (0.41% at 723 nm).
