Fine mapping of ClLOX, a QTL for powdery mildew resistance in watermelon (Citrullus lanatus L.).

KEY MESSAGE: ClLOX, is located on chromosome 2 and encodes a lipoxygenase gene, which induced watermelon powdery mildew resistance by inhibiting pathogen spread. Powdery mildew is one of the most severe fungal diseases reducing yield and quality of watermelon (Citrullus lanatus L.) and other cucurbit crops. Genes responsible for powdery mildew resistance in watermelon are highly valuable. In this study, we first identified the QTL pm-lox for powdery mildew resistance in watermelon, located within a 0.93 Mb interval of chromosome 2, via XP-GWAS method using two F2 populations. The F2:3 families from one of the F2 populations were then used for fine-mapping the pm-lox locus into a 9,883 bp physical region between 29,581,906 and 29,591,789, containing only two annotated genes. Of these, only ClG42_02g0161300 showed a significant differential expression between the resistant and susceptible lines after powdery mildew inoculation based on RNA sequencing (RNA-seq) and qRT-PCR analysis, and is designated ClLOX. Derived Cleaved Amplified Polymorphic Sequence (dCAPs) markers were developed and validated. In addition, our tests showed that the resistance was anti-spread rather than anti-infection of the pathogen. This study identified a new resistance gene (ClLOX), provided insights into the mechanism of powdery mildew resistance, and developed a molecular marker for watermelon breeding.

Real-time ghost imaging algorithm on the multidimensional vector matrix Walsh transformation with spatiotemporal free-fps.

In this research, we propose a real-time spatiotemporal free-fps algorithm based on the multidimensional vector matrix Walsh transform with an adjustable ghost imaging video quality and a frame rate to address the issues of uneven imaging speed, fixed imaging frame rate, and uncomfortable appearance in real-time ghost imaging videos of moving objects. This algorithm utilizes the temporal and spatial correlation of ghost imaging videos to achieve free and adjustable video frame rates in time without being limited by DMD refresh rates. Improving the spatial information of a single frame in space enhances the smoothness of ghost imaging videos, making the appearance of ghost imaging videos more comfortable. To achieve this, a four-dimensional vector Walsh transform kernel matrix is used to transform and reconstruct the high-quality images of the target object. Then the reconstructed high-quality image is spatially interpolated to enhance spatial information. Reasonable frame rate parameters are set based on the corresponding relationship between the detection values of the adjacent frames and speckle, improving the ghost imaging video in both time and space and achieving a smooth real-time ghost imaging video with an adjustable quality and frame rate. The simulation and experimental results of moving objects show that our algorithm solves the limitation of a DMD refresh rate compared with the existing ghost imaging video methods and makes the ghost imaging video more comfortable and smoother in real time. The PSNR of the objective evaluation index is increased by 12%. Regarding a subjective evaluation, this paper proposes an adaptive parameterless evaluation algorithm (APEA) for images with different resolutions based on the NRSS, which improved the structure retention degree by 13% and the Brisque parameter evaluation by 70%. We propose an adaptive parameterless video comfort evaluation algorithm (APVCEA) to evaluate the subjective comfort of ghost imaging videos by 14% compared with traditional methods.

Firm value in the airline industry: perspectives on the impact of sustainability and Covid-19.

To date, there has been limited research undertaken into firm value determinants in the air transport industry, one of the most essential sectors for global business. In view of this, in this study, we review and synthesise the literature that focuses on the value of firms in this sector and discuss conceptually and empirically the determinants influencing airlines' stock values. Our main objective is to widen our understanding of the current state of research on the firm value of air transport companies. Using the systematic literature review (SLR) approach, we classify 173 papers published from 1984 to 2021. We find considerable changes in academic interest in the topic over the time period analysed, especially as a consequence of crisis-induced market crashes. In addition, we classify the main research themes relating to airlines' market value, identify gaps, and introduce potential future research avenues in this area. Among the themes identified, the adjustment in the industry-level factors such as alliances, market structure and competition were the most common source of fluctuations in airlines' stock value. However, we find shifting to sustainability initiatives and its consequence for stakeholders' value as one of the most discussed topics in this context. The trend has gained attention since early 2020 due to the emergence of the Covid-19 pandemic as companies are looking for green and sustainable ways to protect the value in crisis time. Our findings assist transportation researchers and executives in addressing major value drivers of airline firms.

Characterization and Expression Analysis of Regeneration-Associated Protein (Aj-Orpin) during Intestinal Regeneration in the Sea Cucumber Apostichopus japonicus.

Apostichopus japonicus achieves intestinal regeneration in a short period after evisceration, and multiple genes are involved in this process. The transcriptome of A. japonicus was screened for regeneration-associated protein (Aj-Orpin), a gene that is specifically upregulated during intestinal regeneration. The expression and function of Aj-Orpin were identified and investigated in this study. The 5' and 3' RACE polymerase chain reaction (PCR) was used to clone the full-length cDNA of Aj-Orpin. The open reading frame codes for a 164 amino-acid protein with an EF-hand_7 domain and overlapping signal peptides and transmembrane regions. Moreover, Aj-Orpin mRNA and protein expression during intestinal regeneration was investigated using real-time quantitative PCR and Western blot. The expression pattern of Aj-Orpin in the regenerating intestine was investigated using immunohistochemistry. The results showed that Aj-Orpin is an exocrine protein with two EF-hand-like calcium-binding domains. Expression levels were higher in the regenerating intestine than in the normal intestine, but protein expression changes lagged behind mRNA expression changes. Aj-Orpin was found to play a role in the formation of blastema and lumen. It was primarily expressed in the serosal layer and submucosa, suggesting that it might be involved in proliferation. These observations lay the foundation for understanding the role of Orpin-like in echinoderm intestinal regeneration.

Effect of γ-irradiation on structure, physicochemical property and bioactivity of soluble dietary fiber in navel orange peel.

Soluble dietary fibers are widely used in functional food. In this work, the effects of γ-irradiation on molecular weight, structure, physicochemical properties and bioactivities of soluble dietary fiber in navel orange peel (OSDF) were investigated. Γ-irradiation enhanced the extraction yield of OSDF. The molar ratio of glucose and galacturonic acid was increased. The molecular weight profile of OSDF was modified. Γ-irradiation (3-6 kGy) improved the water holding capacity, water swelling capacity, oil holding capacity, cation-exchange capacity, nitrite adsorption capacity and total antioxidant capacity of OSDF. Glucose adsorption capacity and bifidobacterium proliferation capacity of OSDF were improved in a dose-dependent behaviour. Moreover, γ-irradiation promoted the cracking of microstructure. FT-IR spectra showed that more carboxyl groups were newly formed by γ-irradiation. These findings indicated that γ-irradiation treatment was an efficient technique for improving physicochemical properties and health benefits.

Determinants of financial distress in the European air transport industry: The moderating effect of being a flag-carrier.

Due to the COVID-induced global collapse in demand for air travel, the year 2020 was a catastrophic one for the aviation industry. A dramatic drop in operating revenues along with continuing fixed expenses drained the cash reserves of airlines, with consequent risks of financial distress and, potentially, even of bankruptcy. Flag-carriers are a special group in the airline business-they are considered to have privileges in terms of the support given by governments while, on the other hand, are often viewed as having low efficiency and performance. This study aims to estimate for European airlines the interaction effect of being a flag-carrier (flagship) with the relationship between leverage, liquidity, profitability, and the degree of financial distress. Findings obtained from analysing 99 European airlines over a period of ten years, indicate that the negative influence of leverage on financial stability is higher in the case of flag carriers (flagship). The impact of liquidity and profitability on financial health is more positive for flagship than for non-flagship carriers. These findings are not limited to contributing to the existing literature, but also have significant practical implications for executives, managers, and policy makers in the European air transport sector.

[Experimental study of repairing rabbit femur defect with NPP/C-HA/ rhBMP-2].

PURPOSE: To investigate the osteogenic effect of nano-grade pearl powder(NPP)/chitosan-hyaluronic acid (C-HA)/recombinant human bone morphology protein-2 (rhBMP-2) artificial bone. METHODS: A bone defect model with a diameter of 7 mm and a height of 10 mm was made at the distal end of the femur. NPP/C-HA stent containing rhBMP-2 was prepared according to the shape of the defect. No material was implanted in the defect as blank group. NPP/C-HA was used as the control group, NPP/C-HA/rhBMP-2 was implanted into the experimental group. At 4 weeks, 8 weeks, and 12 weeks, the bone effects of each component were detected by cone-beam CT(CBCT), H-E and Masson staining. Serum ALP activity and OCN in tissues to determine the osteogenic differentiation and osteogenesis maturity were detected. SPSS 18.0 software package was used for statistical analysis. RESULTS: At 12 weeks, the defect was completely repaired in the experimental group. No immunological side effects such as inflammation and rejection were observed. At 8 and 12 weeks, CBCT showed that the experimental group had a higher CT value (Hounsfield units, HU) compared with the control group and the blank group(P<0.05). H-E and Masson staining showed that the experimental group had obvious new bone formation compared with the control group and the blank group at 8 weeks and 12 weeks, and ALP activity of the experimental group was significantly different from the control group and the blank group at 8 weeks. OCN immunohistochemical scoring of the experimental group was significantly different from the control group and the blank group(P<0.05). CONCLUSIONS: NPP/C-HA/rhBMP-2 has good tissue fusion, osteoinductivity, osteoconductivity and osteogenicity, which is expected to provide more effective treatment for bone repair.

Comparison of Myocardial Layer-Specific Strain and Global Myocardial Work Efficiency During Treadmill Exercise Stress in Detecting Significant Coronary Artery Disease.

BACKGROUND: Myocardial layer-specific strain can identify myocardial ischemia. Global myocardial work efficiency (GWE) based on non-invasive left ventricular (LV) pressure-strain loops is a novel parameter to determine LV function considering afterload. The study aimed to compare the diagnostic value of GWE and myocardial layer-specific strain during treadmill exercise stress testing to detect significant coronary artery disease (CAD) with normal baseline wall motion. METHODS: Eighty-nine patients who referred for coronary angiography due to suspected of CAD were included. Forty patients with severe coronary artery stenosis were diagnosed with significant CAD, and 49 were defined as non-significant CAD. Stress echocardiography was performed 24 h before angiography. Layer-specific longitudinal strains were assessed from the endocardium, mid-myocardium, and epicardium by 2D speckle-tracking echocardiography. Binary logistic regression analyses were performed to evaluate the association between significant CAD and echocardiographic parameters. A receiver operating characteristic curve was used to assess the capability of layer-specific strain and GWE to diagnose significant CAD. RESULTS: Patients with significant CAD had the worse function in all three myocardial layers at peak exercise compared with those with non-significant CAD when assessed with global longitudinal strain (GLS). At the peak exercise and recovery periods, GWE was lower in patients with significant CAD than in patients with non-significant CAD. In multivariable binary logistic regression analysis, peak endocardial GLS (OR: 1.35, p = 0.006) and peak GWE (OR: 0.76, p = 0.001) were associated with significant CAD. Receiver operating characteristic curves showed peak GWE to be superior to mid-myocardial, epicardial, and endocardial GLS in identifying significant CAD. Further, adding peak GWE to endocardial GLS could improve diagnostic capabilities. CONCLUSIONS: Both GWE and endocardial GLS contribute to improving the diagnostic performance of exercise stress echocardiography. Furthermore, adding peak GWE to peak endocardial GLS provides incremental diagnostic value during a non-invasive screening of significant CAD before radioactive or invasive examinations.

Board effects on innovation in family and non-family business.

This paper contributes to the corporate governance and innovation literature by providing empirical evidence with respect to the influence of composition of the board and its leadership structure on innovation. Also, this study seeks to investigate if such influence differs when comparing family and non-family business. Data were collected from 86 Spanish companies of innovative sectors from 2003 to 2014. The results show that innovation is affected positively by board size, especially in the case of family businesses, and gender diversity, especially in non-family businesses. Similarly, findings also point out that duality is better than the independence of functions in the case of non-family businesses. Finally, obtained results support that independent directors have a negative impact on innovation and such negative influence is even stronger in family firms. These findings contribute to an inconclusive literature regarding board effects on innovation, highlighting different recommendations depending on whether the companies are family businesses or not.

The Stability and Survival Rate of Dental Implants After Preparation of the Site by Piezosurgery vs Conventional Drilling: A Systematic Review and Meta-Analysis.

PURPOSE: This study aimed to assess the stability and survival rate of dental implants inserted with different site preparation techniques-piezoelectric inserts versus traditional rotary instruments. MATERIALS AND METHODS: Correlative research was located by searching articles in PubMed, EMBASE, and the Cochrane Library. This was accomplished independently by two different reviewers and supplemented by a manual search. Only prospective studies evaluating piezoelectric vs conventional implant site preparation in dental implantation were included in this review. A meta-analysis was performed on the stability and survival rate of implants. RESULTS: One thousand fifty-five articles were identified following the search strategy, of which five studies were finally included in this meta-analysis. With regard to the survival rate of implants, there was no statistically significant difference between piezoelectric and conventional implant site preparation (RR = 0.98, 95% CI: 0.94, 1.03; I2 = 0%, P = .86). On the other side, the piezoelectric group had better stability in the eighth week (MD = 4.24, 95% CI: 1.36, 7.12; I2 = 0%, P = .80) and 12th week (MD = 3.33, 95% CI: 0.59, 6.08; I2 = 0%, P = .87) compared with the conventional group. CONCLUSION: Within the limitations of this study, it suggests that the survival rate of implants may not be influenced by the site preparation techniques (piezoelectric vs conventional), but the piezoelectric group may achieve better stability than the conventional group.

Nano-pearl powder/chitosan-hyaluronic acid porous composite scaffold and preliminary study of its osteogenesis mechanism.

Bone tissue engineering using osteoinductive scaffolds has evolved into a promising approach for bone regeneration. Pearl powder has recently gained interest in bone regeneration due to their bioactive characteristics and favorable mechanical properties. In order to mimic bone tissue structurally and compositionally, chitosan-hyaluronic acid (C-HA) scaffolds containing nano-pearl powder (NPP) were fabricated in current study by using freeze-drying method. The microstructure, porosity, hydrophilia, and mechanical property of scaffolds were studied by scanning electron microscopy (SEM), mercury porosimeter, contact angles, and universal material experiment machine respectively. The crystal form of NPP, which was packed in scaffolds, was tested by X-ray diffraction (XRD), and the interaction between three constituents was studied by Fourier transformed infrared (FTIR). In the other hand, the biocompatibility, osteogenic characteristics, and osteogenic related gene expression of scaffolds were examined by cell counting kit-8 (CCK-8), alkaline phosphatase activity (ALP), RT-qPCR, and Western blotting. The results revealed that the NPP was successfully incorporated in the C-HA scaffolds. The hydrophilia and mechanical properties of scaffolds increased with NPP content, whereas the pore morphology was disturbed, especially for 25% scaffold. The scaffolds had a high porosity between 89% and 93%, and decreased slightly with the increase of NPP. Cell culture tests indicated that scaffolds with higher NPP proportion were beneficial for the proliferation and differentiation of MC3T3-E1 cells, and scaffolds with 10 wt% and 25 wt% nano-pearl powder were most effective. The differentiation might be promoted by upregulating the expression of Col αI, OCN, OPN and Runx2 genes. Therefore, the nano-pearl powder/chitosan-hyaluronic acid (NPP/C-HA) scaffolds may serve as a promising biomaterial for bone tissue engineering.

Impacts of RETN genetic polymorphism on breast cancer development.

The adipokine resistin is linked with obesity, inflammation and various cancers, including breast cancer. This study sought to determine whether certain polymorphisms in the gene encoding resistin, RETN, increase the risk of breast cancer susceptibility. We analyzed levels of resistin expression in breast cancer tissue and samples from The Cancer Genome Atlas database. We also examined associations between four RETN single nucleotide polymorphisms (SNPs; rs3745367, rs7408174, rs1862513 and rs3219175) and breast cancer susceptibility in 515 patients with breast cancer and 541 healthy women without cancer. Compared with wild-type (GG) carriers, those carrying the AG genotype of the RETN SNP rs3219175 and those carrying at least one A allele in the SNP rs3219175 had a higher chance of developing breast cancer (adjusted odds ratio, AOR: 1.295, 95% confidence intervals, CI: 1.065-1.575 and 2.202, 1.701-2.243, respectively). When clinical aspects and the RETN SNP rs7408174 were examined in the breast cancer cohort, the CT genotype was linked to late-stage disease, while women with luminal A disease and at least one C allele were likely to progress to stage III/IV disease and to develop highly pathological grade III disease. Moreover, resistin-positive individuals were at greater risk than resistin-negative individuals for developing pathological grade III disease (OR: 5.020; 95% CI: 1.380-18.259). This study details risk associations between resistin and RETN SNPs in breast cancer susceptibility in Chinese Han women.

A bibliometric study on intelligent techniques of bankruptcy prediction for corporate firms.

Bibliometric analysis is an effective method to carry out quantitative study of academic output to address the research trends on a given area of investigation through analysing existing documents. This paper aims to explore the application of intelligent techniques in bankruptcy predictions so as to assess its progress and describe the research trend through bibliometric analysis over the last five decades. The results indicate that, although there is a significant increase in publication number since the 2008 financial crisis, the collaboration among authors is weak, especially at the international dimension. Also, the findings provide a comprehensive view of interdisciplinary research on bankruptcy modelling in finance, business management and computer science fields. The authors sought to contribute to the theoretical development of bankruptcy prediction modeling by bringing new knowledge and key insights. Artificial intelligent techniques are now serving as important alternatives to statistical methods and demonstrate very promising results. This paper has both theoretical and practical implications. First, it provides insights for scholars into the theoretical evolution and intellectual structure for conducting future research in this field. Second, it sheds light on identifying under-explored machine learning techniques applied in bankruptcy prediction which can be crucial in management and decision-making for corporate firm managers and policy makers.

De Novo assembly and comparative transcriptome analyses of purple and green morphs of Apostichopus japonicus during body wall pigmentation process.

Pigmentation processes provide a traceable and relevant trait for understanding key issues in evolutionary biology such as adaptation, speciation and the maintenance of balanced polymorphisms. The sea cucumber Apostichopus japonicus, which has nutritive and medical properties, is considered the most valuable commercial species in many parts of Asia. Compared with the green morph, the purple morph is rare and has great appeal to consumers. However, little is currently known about the molecular mechanism of body color formation in A. japonicus, even in echinoderm. Here, we employ illumina sequencing to examine expression patterns of the gene network underlying body wall development in purple and green morphs of A. japonicus. Overall, the number of down-regulated genes in the green morph was significantly more than in the purple morph during the pigmentation stage. We observed dynamic expression patterns of a large number of pigment, regulation and growth genes from the "Melanogenesis", "Melanoma", "Wnt signaling pathway", "Notch signaling pathway", "epithelium development", "epidermal growth factor receptor binding","growth factor activity" and "growth", including contrasting expression patterns of these genes in green and purple morph. This study provides comprehensive lists of differentially expressed genes during body wall development in the green and purple morphs, revealing potential candidate genes that may be involved in regulating body color formation and polymorphism. These data will provide valuable information for future genetic studies on sea cucumbers elucidating the molecular mechanisms underlying pigmentation, and may support the culture of desirable color morphs.

Comparative Phospho- and Acetyl Proteomics Analysis of Posttranslational Modifications Regulating Intestine Regeneration in Sea Cucumbers.

Sea cucumbers exposed to stressful circumstances eviscerate most internal organs, and then regenerate them rapidly under favorable environments. Reversible protein phosphorylation and acetylation are major modifications regulating protein function. Herein, for the first time, we perform quantitative phospho- and acetyl proteomics analyses of intestine regeneration in a sea cucumber species Apostichopus japonicus. We identified 1,862 phosphorylation sites in 1,169 proteins, and 712 acetylation sites in 470 proteins. Of the 147 and 251 proteins differentially modified by phosphorylation and acetylation, respectively, most were related to cytoskeleton biogenesis, protein synthesis and modification, signal recognition and transduction, energy production and conversion, or substance transport and metabolism. Phosphorylation appears to play a more important role in signal recognition and transduction than acetylation, while acetylation is of greater importance in posttranslational modification, protein turnover, chaperones; energy production and conversion; amino acid and lipid transport and metabolism. These results expanded our understanding of the regulatory mechanisms of posttranslational modifications in intestine regeneration of sea cucumbers after evisceration.

IL-13 -1112 polymorphism and periodontitis susceptibility: a meta-analysis.

BACKGROUND: Several studies have examined the association between the IL-13 -1112C/T polymorphism and the risk of periodontitis. However, these studies have reached different conclusions. The aim of the current study was to investigate the link between this IL-13 -1112 polymorphism and susceptibility to periodontitis. METHODS: We utilized electronic databases, including the CNKI (China National Knowledge Infrastructure), Wanfang, PubMed, Embase, and Cochrane Library databases, to manually search for relevant research published through November 30, 2016. The Chinese and English terms used to search the literature included "periodontitis", "periodontal disease", "IL 13", "IL-13", and "interleukin-13". In accordance with our inclusion criteria, we selected studies that involved case-control trials. All of these case-control trials described their objectives, design and specific statistical methods. For all included studies, odds ratios (ORs) and 95% confidence intervals (95% CIs) were provided or could be calculated from the study data. The quality of the included literature was evaluated using the Newcastle-Ottawa scale (NOS). STATA 12.0 was used to calculate the sizes of the combined effects and conduct a sensitivity analysis of the results. RESULTS: Our meta-analysis included 4 articles representing 5 case-control studies with a total of 710 cases and 671 control subjects. The meta-analysis results indicated that the CC vs TT model, CT vs TT model and TT vs CT + CC model (CC VS TT: OR = 0.615, 95% CI = 0.395-0.957; CT vs TT: OR = 0.518, 95% CI = 0.323-0.830; and TT vs CT + CC: OR = 1.739, 95% CI = 1.130-2.676) were significant in five IL-13 -1112 gene polymorphism and periodontitis susceptibility models. Subgroup analysis indicated that the CC vs TT, CT vs TT and TT vs CT + CC models were significant in the chronic periodontitis (CP) group, whereas no significant differences were found in the five aggressive periodontitis (AgP) group models. The sensitivity analysis showed that dropping any single study did not affect the pooled analysis results. CONCLUSION: The IL-13 -1112 polymorphism may be associated with susceptibility to periodontitis. The IL-13 -1112 gene polymorphism may be associated with susceptibility to CP but not to AgP. Thus, large-scale, multi-ethnic case-control trials are still warranted.

EGFR conjunct FSCN1 as a Novel Therapeutic Strategy in Triple-Negative Breast Cancer.

Emerging evidence indicates that Fascin-1 (FSCN1) may possess a causal role in the development of several types of cancers and serves as a novel biomarker of aggressiveness in certain carcinomas. However, the regulatory mechanism of FSCN1 in triple-negative breast cancer (TNBC) cell invasion and migration is still largely unknown. In our study, we observed that the FSCN1 expression rates were significantly higher in invasive ductal carcinoma, compared with both usual ductal hyperplasia and ductal carcinoma in situ. FSCN1 expression was significantly higher in cases of TNBC compared with the non-TNBC subtype. Overexpression of FSCN1 promoted TNBC cell migration and invasion. Epidermal growth factor induced the expression of FSCN1 through activation of MAPK, which subsequently promoted cell migration and invasion. A significant decrease in FSCN1 expression following the co-treatment of FSCN1 siRNA and Gefitinib, compared with the separate treatment of FSCN1 siRNA or Gefitinib. Furthermore, we found that there was a significant association between FSCN1 expression and poor relapse-free survival and overall survival. Therefore, we suggest that co-targeting epidermal growth factor receptor and FSCN1 dual biomarker may be used as a novel therapeutic strategy for TNBC.

FSCN1 gene polymorphisms: biomarkers for the development and progression of breast cancer.

Breast cancer is a major cause of cancer mortality worldwide. Fascin-1 (FSCN1) is an actin-binding protein found in mammalian cells, including endothelial, neuronal and mesenchymal cells. FSCN1 overexpression has been indicated in breast cancer patients. However, scant information is available regarding the association between FSCN1 single nucleotide polymorphisms (SNPs) and the risk or prognosis of breast cancer. We report on the association between 6 SNPs of the FSCN1 gene (rs56156320, rs8772, rs3801004, rs2966447, rs852479 and rs1640233) and breast cancer susceptibility as well as clinical outcomes in 316 patients with breast cancer and in 222 healthy controls. Carriers of the AC or AC + CC allele of the variant rs56156320 were at greater risk of breast cancer compared with wild-type (AA) carriers. Moreover, carriers of at least one G allele in rs3801004 were likely to progress to stage III/IV disease and lymph node metastasis. Individuals with at least one T allele at FSCN1 SNP rs2966447 were at higher risk of developing pathologic grade G3 disease. Furthermore, individuals bearing the C/C haplotype at SNPs rs56156320 and rs3801004 had nearly twice the risk of breast cancer. Our results indicate that genetic variations in the FSCN1 gene may serve as an important predictor of early-stage breast cancer.

The sea cucumber genome provides insights into morphological evolution and visceral regeneration.

Apart from sharing common ancestry with chordates, sea cucumbers exhibit a unique morphology and exceptional regenerative capacity. Here we present the complete genome sequence of an economically important sea cucumber, A. japonicus, generated using Illumina and PacBio platforms, to achieve an assembly of approximately 805 Mb (contig N50 of 190 Kb and scaffold N50 of 486 Kb), with 30,350 protein-coding genes and high continuity. We used this resource to explore key genetic mechanisms behind the unique biological characters of sea cucumbers. Phylogenetic and comparative genomic analyses revealed the presence of marker genes associated with notochord and gill slits, suggesting that these chordate features were present in ancestral echinoderms. The unique shape and weak mineralization of the sea cucumber adult body were also preliminarily explained by the contraction of biomineralization genes. Genome, transcriptome, and proteome analyses of organ regrowth after induced evisceration provided insight into the molecular underpinnings of visceral regeneration, including a specific tandem-duplicated prostatic secretory protein of 94 amino acids (PSP94)-like gene family and a significantly expanded fibrinogen-related protein (FREP) gene family. This high-quality genome resource will provide a useful framework for future research into biological processes and evolution in deuterostomes, including remarkable regenerative abilities that could have medical applications. Moreover, the multiomics data will be of prime value for commercial sea cucumber breeding programs.

Differential Expression of miRNAs in the Respiratory Tree of the Sea Cucumber Apostichopus japonicus Under Hypoxia Stress.

The sea cucumber, an important economic species, has encountered high mortality since 2013 in northern China because of seasonal environmental stress such as hypoxia, high temperature, and low salinity. MicroRNAs (miRNAs) are important in regulating gene expression in marine organisms in response to environmental change. In this study, high-throughput sequencing was used to investigate alterations in miRNA expression in the sea cucumber under different levels of dissolved oxygen (DO). Nine small RNA libraries were constructed from the sea cucumber respiratory trees. A total of 26 differentially expressed miRNAs, including 12 upregulated and 14 downregulated miRNAs, were observed in severe hypoxia (DO 2 mg/L) compared with mild hypoxia (DO 4 mg/L) and normoxic conditions (DO 8 mg/L). Twelve differentially expressed miRNAs were clustered in severe hypoxia. In addition, real-time PCR revealed that 14 randomly selected differentially expressed miRNAs showed significantly increased expressions in severe hypoxia and the expressions of nine miRNAs, including key miRNAs such as Aja-miR-1, Aja-miR-2008, and Aja-miR-184, were consistent with the sequencing results. Moreover, gene ontology and pathway analyses of putative target genes suggest that these miRNAs are important in redox, transport, transcription, and hydrolysis under hypoxia stress. Notably, novel-miR-1, novel-miR-2, and novel-miR-3 were specifically clustered and upregulated in severe hypoxia, which may provide new insights into novel "hypoxamiR" identification. These results will provide a basis for future studies of miRNA regulation and molecular adaptive mechanisms in sea cucumbers under hypoxia stress.