"Slow down. Rail crossing ahead. Look left and right at the crossing": In-vehicle auditory alerts improve driver behavior at rail crossings.

Even though the rail industry has made great strides in reducing accidents at crossings, train-vehicle collisions at Highway-Rail Grade Crossings (HRGCs) continue to be a major issue in the US and across the world. In this research, we conducted a driving simulator study (N = 35) to evaluate a hybrid in-vehicle auditory alert (IVAA), composed of both speech and non-speech components, that was selected after two rounds of subjective evaluation studies. Participants drove through a simulated scenario and reacted to HRGCs with and without the IVAA present and through different music conditions and crossing devices. Driver simulator testing results showed that the inclusion of the hybrid IVAA significantly improved driving behavior near HRGCs in terms of gaze behavior, braking reaction, and approach speed to the crossing. The driving simulator study also showed the effects of background music and warning device types on driving performance. The study contributes to the large-scale implementation of IVAAs at HRGCs, as well as the development of guidelines toward a more standardized approach for IVAAs at HRGCs.

Centromeric DNA characterization in the model grass Brachypodium distachyon provides insights on the evolution of the genus.

Brachypodium distachyon is a well-established model monocot plant, and its small and compact genome has been used as an accurate reference for the much larger and often polyploid genomes of cereals such as Avena sativa (oats), Hordeum vulgare (barley) and Triticum aestivum (wheat). Centromeres are indispensable functional units of chromosomes and they play a core role in genome polyploidization events during evolution. As the Brachypodium genus contains about 20 species that differ significantly in terms of their basic chromosome numbers, genome size, ploidy levels and life strategies, studying their centromeres may provide important insight into the structure and evolution of the genome in this interesting and important genus. In this study, we isolated the centromeric DNA of the B. distachyon reference line Bd21 and characterized its composition via the chromatin immunoprecipitation of the nucleosomes that contain the centromere-specific histone CENH3. We revealed that the centromeres of Bd21 have the features of typical multicellular eukaryotic centromeres. Strikingly, these centromeres contain relatively few centromeric satellite DNAs; in particular, the centromere of chromosome 5 (Bd5) consists of only ~40 kb. Moreover, the centromeric retrotransposons in B. distachyon (CRBds) are evolutionarily young. These transposable elements are located both within and adjacent to the CENH3 binding domains, and have similar compositions. Moreover, based on the presence of CRBds in the centromeres, the species in this study can be grouped into two distinct lineages. This may provide new evidence regarding the phylogenetic relationships within the Brachypodium genus.