hUCMSC-derived exosomes protect against GVHD-induced endoplasmic reticulum stress in CD4+ T cells by targeting the miR-16-5p/ATF6/CHOP axis.

Exosomes generated from mesenchymal stem cells (MSCs) are thought to be a unique therapeutic strategy for several autoimmune deficiency illnesses. The purpose of this study was to elucidate the protective effects of human umbilical cord mesenchymal stem cell-derived exosomes (hUCMSC-Exo) on CD4+ T cells dysfunction during graft-versus-host disease (GVHD) and to identify the underlying processes involved. Here, we showed that hUCMSC-Exo treatment can effectively attenuate GVHD injury by alleviating redox metabolism disorders and inflammatory cytokine bursts in CD4+ T cells. Furthermore, hUCMSC-Exo ameliorate ER stress and ATF6/CHOP signaling-mediated apoptosis in CD4+ T cells and promote the development of CD4+IL-10+ T cells during GVHD. Moreover, downregulating miR-16-5p in hUCMSC-Exo impaired their ability to prevent CD4+ T cells apoptosis and weakened their ability to promote the differentiation of CD4+IL-10+ T cells. Collectively, the obtained data suggested that hUCMSC-Exo suppress ATF6/CHOP signaling-mediated ER stress and apoptosis in CD4+ T cells, enhance the differentiation of CD4+IL-10+ T cells, and reverse the imbalance of immune homeostasis in the GVHD process by transferring miR-16-5p. Our study provided further evidence that GVHD patients can benefit from hUCMSC-Exo-mediated therapy.

Switchable Two-Dimensional AND and Exclusive OR Operation Based on Dual-Wavelength Metasurfaces.

Logic operation serves as the foundation and core element of computing networks; it will bring huge vitality to advanced information processing with its adaptation in the optical domain. As fundamental logic operations, AND and exclusive OR (XOR) operations serve a multitude of purposes, such as their ability to cooperate in enabling image processing and interpretation. Here, we propose and experimentally demonstrate a wavelength multiplexed AND and XOR function based on metasurfaces. By combining two cosine gratings with distinct frequencies and an initial phase difference of π/2, we extract the similarities and differences between two input images simultaneously by illuminating them with 445 and 633 nm wavelengths. Additionally, we explore its potential in information encryption, where overall security is enhanced by distributing distinct parts of initial information and encoded keys to different receivers. This design possesses the benefits of convenient mode switching and high-quality imaging, facilitating advanced applications in pattern recognition, machine vision, medical diagnosis, etc.

Perovskite single-pixel detector for dual-color metasurface imaging recognition in complex environment.

Highly efficient multi-dimensional data storage and extraction are two primary ends for the design and fabrication of emerging optical materials. Although metasurfaces show great potential in information storage due to their modulation for different degrees of freedom of light, a compact and efficient detector for relevant multi-dimensional data retrieval is still a challenge, especially in complex environments. Here, we demonstrate a multi-dimensional image storage and retrieval process by using a dual-color metasurface and a double-layer integrated perovskite single-pixel detector (DIP-SPD). Benefitting from the photoelectric response characteristics of the FAPbBr2.4I0.6 and FAPbI3 films and their stacked structure, our filter-free DIP-SPD can accurately reconstruct different colorful images stored in a metasurface within a single-round measurement, even in complex environments with scattering media or strong background noise. Our work not only provides a compact, filter-free, and noise-robust detector for colorful image extraction in a metasurface, but also paves the way for color imaging application of perovskite-like bandgap tunable materials.

Phase-assisted Bessel-metasurface: a single-sized approach for simultaneous printing and holography.

Due to the unprecedented wavefront shaping capability, the metasurface has demonstrated state-of-the-art performances in various applications, especially in printing and holography. Recently, these two functions have been combined into a single metasurface chip to achieve a capability expansion. Despite the progress, current dual-mode metasurfaces are realized at the expense of an increase in the difficulty of the fabrication, reduction of the pixel resolution, or strict limitation in the illumination conditions. Inspired by the Jacobi-Anger expansion, a phase-assisted paradigm, called Bessel metasurface, has been proposed for simultaneous printing and holography. By elaborately arranging the orientations of the single-sized nanostructures with geometric phase modulation, the Bessel metasurface can not only encode a greyscale printing image in real space but can reconstruct a holographic image in k-space. With the merits of compactness, easy fabrication, convenient observation, and liberation of the illumination conditions, the design paradigm of the Bessel metasurface would have promising prospects in practical applications, including optical information storage, 3D stereoscopic displays, multifunctional optical devices, etc.

All-Optical Multiplexed Meta-Differentiator for Tri-Mode Surface Morphology Observation.

Current optical differentiators are generally limited to realizing a single differential function once fabricated. Herein, a minimalist strategy in designing multiplexed differentiators (1st - and 2nd -order differentiations), implemented with a Malus metasurface consisting of single-sized nanostructures is proposed, thus improving the functionality of optical computing devices without the cost of complex design and nanofabrication. It is found that the proposed meta-differentiator exhibits excellent differential-computation performance and can be used for simultaneous outline detection and edge positioning of objects, corresponding to the functions of the 1st - and 2nd -order differentiations respectively. Experiments with biological specimens showcase that boundaries of biological tissues can not only be identified, but also the edge information for realizing high-precision edge positioning is highlighted. The study provides a paradigm in designing all-optical multiplexed computing meta-devices, and initiates tri-mode surface morphology observation by combining meta-differentiator with optical microscopes, which can find their applications in advanced biological imaging, large-scale defect detection, and high-speed pattern recognition, etc.

Protective effect of hydroxysafflor yellow A on renal ischemia­-reperfusion injury by targeting the Akt­Nrf2 axis in mice.

Ischemic/reperfusion (I/R) injury is the primary cause of acute kidney injury (AKI). Hydroxysafflor yellow A (HSYA), a natural compound isolated from Carthamus tinctorius L., has been found to possess anti-inflammatory and antioxidant properties. However, the protective effects and potential mechanism of HSYA on I/R-induced AKI remains unclear. In the present study, the in vitro hypoxia/reoxygenation (H/R) and in vivo renal I/R models were employed to investigate the renal protective effects and molecular mechanisms of HSYA on I/R-induced AKI. The present results indicated that HSYA pretreatment significantly ameliorated renal damage and dysfunction in the I/R injury mice via enhancing the antioxidant capacity and suppressing the oxidative stress injury, inflammatory response, and apoptosis. Mechanistic studies showed that HSYA could upregulate Akt/GSK-3ß/Fyn-Nrf2 axis-mediated antioxidant gene expression both in vitro and in vivo. Moreover, HSYA-mediated improvement in antioxidant, anti-inflammatory, and anti-apoptotic effects in H/R-treated HK-2 cells was abrogated by Akt inhibitor LY294002 supplementation. In summary, the present results demonstrated that HSYA attenuated kidney oxidative stress, inflammation response, and apoptosis induced by I/R, at least in part, via activating the Akt/GSK-3ß/Fyn-Nrf2 axis pathway. These findings provided evidence that HSYA may be applied as a potential therapeutic agent in the treatment of I/R induced AKI.

Phase-assisted angular-multiplexing nanoprinting based on the Jacobi-Anger expansion.

Featuring with ultracompactness and subwavelength resolution, metasurface-assisted nanoprinting has been widely researched as an optical device for image display. It also provides a platform for information multiplexing, and a series of multiplexed works based on incident polarizations, operating wavelengths and observation angles have emerged. However, the angular-multiplexing nanoprinting is realized at the cost of image resolution reduction or the increase of fabrication difficulty, hindering its practical applications. Here, inspired by the Jacobi-Anger expansion, a phase-assisted design paradigm, called Bessel metasurface, was proposed for angular multiplexing nanoprinting. By elaborately designing the phase distribution of the Bessel metasurface, the target images can be encoded into the desired observation angles, reaching angular multiplexing. With the merits of ultracompactness and easy fabrication, we believe that our design strategy would be attractive in the real-world applications, including optical information storage, encryption/concealment, multifunctional switchable optical devices, and 3D stereoscopic displays, etc.

Dual-channel anticounterfeiting color-nanoprinting with a single-size nanostructured metasurface.

Metasurface-based structural-colors are usually implemented by changing the dimensions of nanostructures to produce different spectral responses. Therefore, a single-size nanostructured metasurface usually cannot display structural-colors since it has only one design degree of freedom (DOF), i.e., the orientation angles of nanostructures. Here, we show structural-color nanoprinting images can be generated with a single-size nanostructured metasurface, enabled by designing the anisotropic nanostructure with different spectral responses along its long- and short-axis directions, respectively. More interestingly, the concept of orientation degeneracy of nanostructures can be applied in the metasurface design, which shows two spectral modulations can be implemented under different polarization directions of output light, thus extending the color-nanoprinting from single-channel to dual-channel. The proposed dual-channel metasurface used for anticounterfeiting color-nanoprinting has presented the advantages of ultra-compactness, high information capacity, and vivid colors, which can develop broad applications in fields such as high-end anticounterfeiting, high-density information storage, optical encryption, etc.

Tri-channel metasurface for watermarked structural-color nanoprinting and holographic imaging.

Structural-color nanoprinting, which can generate vivid colors with spatial resolution at subwavelength level, possesses potential market in optical anticounterfeiting and information encryption. Herein, we propose an ultracompact metasurface with a single-cell design strategy to establish three independent information channels for simultaneous watermarked structural-color nanoprinting and holographic imaging. Dual-channel spectrum manipulation and single-channel phase manipulation are combined together by elaborately introducing the orientation degeneracy into the design of variable dielectric nanobricks. Hence, a structural-color nanoprinting image covered with polarization-dependent watermarks and a holographic image can be respectively generated under different decoded environments. The proposed metasurface shows a flexible method for tri-channel image display with high information capacity, and exhibits dual-mode anticounterfeiting with double safeguards, i.e., polarization-controlled watermarks and a far-field holographic image. This study provides a feasible route to develop multifunctional metasurfaces for applications including optical anticounterfeiting, information encryption and security, information multiplexing, etc.

Multifold Integration of Printed and Holographic Meta-Image Displays Enabled by Dual-Degeneracy.

By virtue of the unprecedented ability of manipulating the optical parameters, metasurfaces open up a new avenue for realizing ultra-compact image displays, e.g., nanoprinting on the surface and holographic displaying in the far-field. The multifold integration of these two functions into a single metasurface can undoubtedly expand the functionality and increase the information capacity. In this study, a minimalist tri-channel metasurface is proposed and experimentally demonstrated with multifold integration of printed and holographic displaying, which can generate two N-bit grayscale images and a four-step holographic image simultaneously. Benefiting from exploiting the degeneracy of energy allocation and the degeneracy of nanostructure orientations, the functionalities of nanoprinting and holography are combined without the need of a large amount of nanostructures with varied dimensions, which would facilitate both the metasurface design and fabrication. The proposed scheme provides a new idea in enhancing the functionality and capacity of metasurfaces without complicating their design, which has promising prospects for applications in ultra-compact image displays, high-density optical storage, optical anti-counterfeiting and many other related fields.

Mass-Manufactured Beam-Steering Metasurfaces for High-Speed Full-Duplex Optical Wireless-Broadcasting Communications.

Beam-steering devices, which are at the heart of optical wireless-broadcasting communication links, play an important role in data allocation and exchange. An ideal beam-steering device features large steering angles, arbitrary channel numbers, reconfigurability, and ultracompactness. However, these criteria have been achieved only partially with conventional beam-steering devices based on waveguides, micro-electricalmechanical systems, spatial light modulators, and gratings, which will substantially limit the application of optical wireless-broadcasting communication techniques. In this study, an ultracompact full-duplex metabroadcasting communication system is designed and experimentally demonstrated, which exhibits beam steering angles up to ±40°, 14 broadcasting channels with capacity for downstream and upstream links up to 100 and 10 Gbps for each user channel, three operating modes for flexible signal switching, and metadevice dimensions as small as 2 mm × 2 mm. In particular, the beam-steering metadevices are mass-manufactured by a complementary metal-oxide-semiconductor (CMOS) processing platform, which shows their potential for large-scale commercial applications. The demonstrated metabroadcasting communication system merges optical wireless-broadcasting communications and metasurfaces, which reduces the complexity of beam-steering devices while significantly increasing their performance, opening up a new avenue for high-quality optical wireless-broadcasting communications.

Metasurface-enabled three-in-one nanoprints by multifunctional manipulations of light.

In metasurface-based ultra-compact image display, color-nanoprints, gray-imaging elements, and binary-pattern-imaging elements are three different types of nanoprints, implemented with different mechanisms of light manipulation. Here, we show the three functional elements can be integrated together to form a "three-in-one" nanoprint with negligible crosstalk, merely with a single-cell nanostructured design approach. Specifically, by decoupling spectrum and polarization-assisted intensity manipulations of incident light, the proposed metasurface appears as a dual-color nanoprint under a broadband unpolarized light source illumination, while simultaneously displaying an independent continuous gray image and another binary-pattern in an orthogonal-polarization optical setup with different polarization controls. Our approach can increase the system integration and security of metasurfaces, which can be of interest to many advanced applications such as data storage, optical information encoding, high-end optical anti-counterfeiting, and optical information hiding.

Metasurfaces with single-sized antennas for reconstructing full-color holographic images without cross talk.

Designing a color hologram with conventional metasurfaces usually resorts to a supercell strategy or single-sized approach with different incident angles. However, these designs still have their own drawbacks that need to be further solved. Herein, we show a new, to the best of our knowledge, single-sized strategy to design full-color geometric meta-holograms by utilizing the conjugation property of two circularly polarized lights with opposite handedness and diffraction dispersion. The experimentally captured holographic color images are reconstructed with high quality and without cross talk, which agrees well with our theoretical prediction. Moreover, only with an appropriate combination of wavelength and polarization state can color images be observed accurately. Our strategy provides a simple and effective approach for full-color meta-holography and offers significant potential in image display, information storage, etc.

Multiplexing meta-hologram with separate control of amplitude and phase.

Metasurfaces have shown their unique capabilities to manipulate the phase and/or amplitude properties of incident light at the subwavelength scale, which provides an effective approach for constructing amplitude-only, phase-only or even complexed amplitude meta-devices with high resolution. Most of meta-devices control the amplitude and/or phase of the incident light with the same polarization state; however, separately controlling of amplitude and phase of the incident light with different polarization states can provide a new degree of freedom for improving the information capacity of metasurfaces and designing multifunctional meta-devices. Herein, we combine the amplitude manipulation and geometric phase manipulation by only reconfiguring the orientation angle of the nanostructure and present a single-sized design strategy for a multiplexing meta-hologram which plays the dual roles: a continuous amplitude-only meta-device and a two-step phase-only meta-device. Two different modulation types can be readily switched merely by polarization controls. Our approach opens up the possibilities for separately and independently controlling of amplitude and phase of light to construct a multiplexing meta-hologram with a single-sized metasurface, which can contribute to the advanced research and applications in multi-folded optical anti-counterfeiting, optical information hiding and optical information encoding.

Asymmetric hologram with a single-size nanostructured metasurface.

Geometric metasurfaces, governed by PB phase, have shown their strong polarization sensitivity and can generate opposite phase delay when the handedness of incident circularly-polarized (CP) light is opposite. Here, we show this interesting characteristic can be employed to generate asymmetric forward and backward propagation with the same incident left- or right-handed CP light, which is hard to achieve with conventional optical elements and devices. Specifically, with the modified holographic design algorithm to consider both forward and backward CP light, an asymmetric meta-hologram is designed, which can project two different holographic images in the forward and backward directions, respectively. We demonstrate this concept by fabricating an asymmetric hologram with a single-size nanostructured metasurface, and the experimentally obtained holographic images in both directions have shown their advantages of high fidelity, broadband response and low crosstalk. The proposed asymmetric metasurface can play an important role in data storages, anti-counterfeitings, optical communications, displays and many other related fields.

3D Meta-Prisms for Versatile Beam Steering by Hybridizing Plasmonic and Diffractive Effect in the Broadband Visible Regime.

As two independent optical sub-fields, diffraction optics and plasmonics both have been used for wavefront shaping and beam steering. However, the two separate concepts have always been developing as two parallel directions, which have not met for studying their structural hybridization to discover new potentials. For instance of the flat metasurfaces, even though the geometric parameters including shape, size, and periodicity have been studied, it remains mostly unexplored for the 3D spatial height variation. Here, a new type of all-metallic 3D meta-prism is proposed and experimentally demonstrated by hybridizing the localized surface plasmonic resonances (LSPR) and the blazed grating diffraction, which enables strong polarization-dependent behaviors to steer broadband visible light to drastically inverse directions. The nanofabrication of 3D meta-prism is achieved by nanostencil lithography with electron-beam evaporation. Such meta-prism could also enable to split different visible light (green, blue, and red) with high-efficiency contrast (≈10). By the mirror-symmetry arrangement, a multifunctional surface is demonstrated with polarization-/wavelength-multiplexing wavefront-shaping functions (concave, convex, or flat mirror). This unique 3D meta-prism enjoys great simplicity and versatility in broadband beam steering through the incorporation of plasmonic and diffractive effects and can be utilized in various applications including dichroic-prism splitters, multifunctional meta-mirrors, etc.

Metasurface-based key for computational imaging encryption.

Optical metasurfaces can offer high-quality multichannel displays by modulating different degrees of freedom of light, demonstrating great potential in the next generation of optical encryption and anti-counterfeiting. Different from the direct imaging modality of metasurfaces, single-pixel imaging (SPI) as a typical computational imaging technique obtains the object image from a decryption-like computational process. Here, we propose an optical encryption scheme by introducing metasurface-images (meta-images) into the encoding and decoding processes as the keys of SPI encryption. Different high-quality meta-images generated by a dual-channel Malus metasurface play the role of keys to encode multiple target images and retrieve them following the principle of SPI. Our work eliminates the conventional digital transmission process of keys in SPI encryption, enables the reusability of a single metasurface in different encryption processes, and thereby paves the way toward a high-security optical encryption between direct and indirect imaging methods.

Single-celled multifunctional metasurfaces merging structural-color nanoprinting and holography.

Nanostructured metasurfaces applied in structural-color nanoprinting and holography have been extensively investigated in the past several years. Recently, merging them together is becoming an emerging approach to improve the information capacity and functionality of metasurfaces. However, current approaches, e.g., segmenting, interleaving and stacking schemes for function merging, suffer from crosstalk, low information density, design and fabrication difficulties. Herein, we employ a single-celled approach to design and experimentally demonstrate a high-density multifunctional metasurface merging nanoprinting and holography, i.e., each nanostructure in the metasurface can simultaneously manipulate the spectra (enabled with varied dimensions of nanostructures) and geometric phase (enabled with varied orientation angles of nanostructures) of incident light. Hence, with different decoding strategies, a structural-color nanoprinting image emerges right at the metasurface plane under white light illumination, while a holographic image is reconstructed in the Fraunhofer diffraction zone under circularly polarized laser light incidence. And the two images have no crosstalk since they are independently designed and presented at different distances. Our proposal suggests a space-multiplexing scheme to develop advanced metasurfaces and one can find their markets in high-density information storage, optical information encryption, multi-channel image display, etc.

Full-space metasurface holograms in the visible range.

Conventional metasurface holography is usually implemented in either transmission space or reflection space. Herein, we show a dielectric metasurface that can simultaneously project two independent holographic images in the transmission and reflection spaces, respectively, merely with a single-layer design approach. Specifically, two types of dielectric nanobricks in a nanostructured metasurface are employed to act as half-wave plates for geometric phase modulation. One type of nanobrick is designed to reflect most of incident circularly-polarized light into reflection space, enabled with magnetic resonance, while another type of nanobrick transmits it into transmission space, without resonance involved. By controlling the orientation angles and randomly interleaving the two types of nanobricks to form a metasurface, a full-space metasurface hologram can be established. We experimentally demonstrate this trans-reflective meta-holography by encoding the geometric phase information of two independent images into a single metasurface, and all observed holographic images agree well with our predictions. Our research expands the field-of-view of metasurface holography from half- to full-space, which can find its markets in optical sensing, image displays, optical storages and many other potential applications.

On-axis three-dimensional meta-holography enabled with continuous-amplitude modulation of light.

Conventional three-dimensional (3D) holography based on recording interference fringes on a photosensitive material usually has unavoidable zero-order light, which merges with the holographic image and blurs it. Off-axis design is an effective approach to avoid this problem; however, it in turn leads to the waste of at least half of the imaging space for holographic reconstruction. Herein, we propose an on-axis 3D holography based on Malus-assisted metasurfaces, which can eliminate the zero-order light and project the holographic image in the full transmission space. Specifically, each nanostructure in the metasurface acts as a nano-polarizer, which can modulate the polarization-assisted amplitude of incident light continuously, governed by Malus law. By carefully choosing the orientation angles of nano-polarizers, the amplitude can be both positive and negative, which can be employed to extinct zero-order light without affecting the intensity modulation for holographic recording. We experimentally demonstrate this concept by projecting an on-axis 3-layer holographic images in the imaging space and all experimental results agree well with our prediction. Our proposed metasurface carries unique characteristics such as ultracompactness, on-axis reconstruction, extinction of zero-order light and broadband response, which can find its market in ultracompact and high-density holographic recording for 3D objects.