ABSTRACT
The production of reactor-based medical isotopes is fragile, which has meant supply shortages from time to time. This paper reviews alternative production methods in the form of cyclotrons, linear accelerators and neutron generators. Finally, the status of the production of medical isotopes in China is described.
Subject(s)
Cyclotrons , Radioisotopes , China , Isotopes , Neutrons , Particle AcceleratorsABSTRACT
INTRODUCTION: We have developed a method for producing no-carrier-added (64)Cu by using chelating resin bearing iminodiacetic acid groups. METHODS: We optimized the conditions for the selective separation of radioactive Cu from Ni and Co using the chelating resin and produced no-carrier-added (64)Cu under the optimized conditions. We analyzed the amounts of the metal ions present in (64)Cu by inductively coupled-plasma mass spectroscopy (ICP-MS) and optical emission spectroscopy (ICP-OES), and performed radiolabeling of monoclonal antibodies in order to investigate the quality of the (64)Cu produced in this study. RESULTS: Radioactive Cu was separated from Ni and Co with 0.1 and 2 M HCl solutions. The yield of (64)Cu isolated from the (64)NiO target was almost 87%. The radiochemical purity of (64)Cu obtained from different amounts of (64)NiO targets was >99% in all cases. We found that the (64)Cu solution presented extremely low amounts of the metal ions and showed high specific activity (average: 595 GBq/mumol). Moreover, the antibodies were labeled with (64)Cu with a high average efficiency (average: 88%). CONCLUSIONS: We could efficiently separate (64)Cu by using short ion-exchange columns. The chelating ion-exchange method provides a high quality of (64)Cu that is sufficient for the synthesis of (64)Cu-labeled antibodies and medical applications.
Subject(s)
Chelating Agents/chemistry , Chromatography, Ion Exchange/methods , Copper Radioisotopes/chemistry , Copper Radioisotopes/isolation & purification , Ion Exchange Resins/chemistry , Antibodies, Monoclonal/metabolism , Cobalt/chemistry , Copper Radioisotopes/metabolism , Imino Acids/chemistry , Isotope Labeling , Mass Spectrometry , Nickel/chemistryABSTRACT
OBJECTIVE: To study the pathogenesis of diabetic cardiomyopathy using DNA microarray. METHODS: Experimental rats were grouped into a diabetic cardiomyopathy and a normal control group. The cDNA probes were prepared by labeling the mRNA extracted from normal tissue and tissue of diabetic cardiomyopathy with Cy3-dUTP and Cy5-dUTP respectively through reverse transcription. DNA microarray were constructed by spotting PCR products of 4 000 rat cDNAs onto a specially treated glass slides, and were then hybridized against the cDNA probes followed by fluorescent signals scanning. RESULTS: The expression of energy metabolism-related genes were lower in the tissues of diabetic cardiomyopathy than in normal tissue. CONCLUSION: The energy metabolism disorder may play an important role in the pathogenesis of diabetic cardiomyopathy.