[Isolation of endophytic fungi from medicinal plant Brucea javanica and their microbial inhibition activity].

OBJECTIVE: To isolate and identify endophytic fungi from Brucea javanica, and to detect the antimicrobial activity of these strains. METHODS: Endophytic fungi were isolated by tissue inoculation culture and identified by conventional morphological characteristic method. Seven kinds of pathogenic fungi and three kinds of bacteria were used as targeting microbes to test microbial inhibition activities by agar plate antagonistic action and modified agar gel diffusion methods, respectively. RESULTS: A total of 83 endophytic fungi strains were isolated from the root, stem, leaf and fruit of Brucea javanica. 34 strains were obtained from the stem, 32 strains were obtained from the leaf, 15 strains were isolated from the root and 2 strains came from the fruit. These 73 strains which had been identified attribute to 5 orders, 6 families and 12 genera. For the isolated strains, 14 strains had antifungal activities against at least one pathogenic fungi, 9 strains showed antibacterial activities against one or more bacteria. Especially, the strain YJ-17 which belonged to Phomopsis genus showed the best inhibitory effect on the targeting microbes. CONCLUSION: The endophytic fungi from Brucea javanica show diversity and microbial inhibition activity, and are worthy for further study on plant disease controlling.

[Quality standard of zhuang medicine jasmini sambacis flos].

OBJECTIVE: To study the qualitative and quantitative determination of Zhuang medicine Jasmini Sambacis Flos and establish its quality control standard. METHODS: Macroscopic, microscopic and TLC identification were adopted to carry out the qualitative identification, the mensuration of inspection items and extractum were according to the Chinese Pharmacopoeia. The contents of quercetin and kaempferide in Jasmini Sambacis Flos were determined by HPLC. RESULTS: The qualitative identification methods had strong specificity. In HPLC quantitative determination, the linear range of quercetin and kaempferide was in the range of 0.4008 - 3.2064 microg and 0.0403 - 3.2256 microg, respectively. CONCLUSION: These methods are simple, accurate and reproducible, and can be used to control the quality of Jasmini Sambacis Flos effectively.

[Research on quality standards of zhuang medicine Lonicerae dasystylae flos].

OBJECTIVE: To establish quality standard of Zhuang medicine Lonicera dasystyla, and provide scientific basis for the quality control of L. dasystyla. METHOD: Characteristics of materia medica, microscopic features, TLC indentification, inspection, extractum and determination of chlorogenic acid, macranthoidin B, dipsacoside B were carried out through the experience, microscopic, physical and chemical methods, respectively. The standard of quality control was formulated thereafter. RESULT: The characteristics of materia medica, microscopic features, TLC indentification were specified, the average contents of water, total ash, acid-insoluble ash, alcohol-soluble extracts, chlorogenic acid were 11.6%, 6.6%, 0.2% , 24.4%, 1.16%, respectively, the total amount of macranthoidin B and dipsacoside B was 3.13%. Quality standard of L. dasystyla was proposed according to experimental results. CONCLUSION: The quality of L. dasystyla can be controlled effectively with the quality standard.

[Study on the pharmacognosy identification and UV identification of Basella rubra].

OBJECTIVE: To provide reference for identification and development of Basella rubra. METHODS: Microscopic identification and UV spectrometry methods were used. RESULTS: Obvious characteristics were found in the tissue structures of the roots, stems and leaves . And Basella rubra showed some UV spectrometry characteristics. CONCLUSION: The results can be used as the reference for analyzing the quality of the crude drug.