ABSTRACT
BACKGROUND: Hyperthermia has been shown promising in the treatment of head and neck squamous cell carcinoma (HNSCC); however, the mechanism underlying hyperthermia reducing tumor metastasis is poorly elucidated. TWIST2, an important transcription factor of epithelial-mesenchymal transition (EMT), plays a critical role in the tumor progression and metastasis. The role of TWIST2 in tongue squamous cell carcinoma (TSCC) and its association with hyperthermia still have not been reported. METHOD: The correlations between TWIST2 expression and the clinical-pathologic characteristics of 89 patients with TSCC were evaluated by immunohistochemical staining. TSCC cell lines transfected with siRNA against TWIST2 were heated for 40 min at 42.5°C, and the migration capability of cells was examined by migration assay. Xenograft tumors in nude mice were treated by hyperthermia, and TWIST2 expression was measured. RESULTS: Our data showed that TWIST2 expression was associated with the metastasis of human TSCC. In Tca8113 and Cal-27 cells, TWIST2-siRNA treatment can reduce cell migration ability and has no effect on the cell proliferation and apoptosis. Hyperthermia can decrease the level of TWIST2 in TSCC and inhibit the migration of cells. CONCLUSIONS: This demonstrated that hyperthermia might decrease the migration of Tca8113 and Cal-27 cells by reducing TWIST2 expression. Altogether, these findings suggest an as yet undescribed link between TWIST2 and hyperthermia in TSCC.
Subject(s)
Carcinoma, Squamous Cell/therapy , Cell Movement/physiology , Head and Neck Neoplasms/therapy , Hyperthermia, Induced/methods , Repressor Proteins/biosynthesis , Tongue Neoplasms/therapy , Twist-Related Protein 1/biosynthesis , Animals , Apoptosis/physiology , Carcinoma, Squamous Cell/genetics , Carcinoma, Squamous Cell/metabolism , Carcinoma, Squamous Cell/pathology , Cell Line, Tumor , Cell Proliferation/physiology , Epithelial-Mesenchymal Transition , Female , Gene Expression Regulation, Neoplastic , Head and Neck Neoplasms/genetics , Head and Neck Neoplasms/metabolism , Head and Neck Neoplasms/pathology , Humans , Mice , Mice, Nude , Neoplasm Metastasis , RNA, Small Interfering/administration & dosage , RNA, Small Interfering/genetics , Random Allocation , Repressor Proteins/genetics , Squamous Cell Carcinoma of Head and Neck , Tongue Neoplasms/genetics , Tongue Neoplasms/metabolism , Tongue Neoplasms/pathology , Transfection , Twist-Related Protein 1/genetics , Xenograft Model Antitumor AssaysABSTRACT
OBJECTIVE: To simulate the expression of TNF-α and PGE2 of periodontal tissues in rat periodontitis model. METHODS: 40 Wistar rats were randomly divided into the periodontitis group and the control group (n=20). After the successful establishment of periodontitis rat model, raising for six weeks before the animals were sacrificed. The periodontal tissues were obtained and made into slices. Observed the histopathological changes of the periodontal tissues and measured TNF-α, PGE2 levels change by immunohistochemistry, Western blot analysis and ELISA. RESULTS: TNF-α, PGE2 expression of the periodontitis group was significantly higher than that in the control group, the difference was significant (P<0.05). CONCLUSIONS: The TNF-α, PGE2 expression of the rat periodontal tissue in the periodontitis group was significantly higher than the control group.
Subject(s)
Dinoprostone/metabolism , Periodontitis/metabolism , Tumor Necrosis Factor-alpha/metabolism , Animals , Dinoprostone/analysis , Disease Models, Animal , Immunohistochemistry , Male , Periodontium/chemistry , Periodontium/metabolism , Rats , Rats, Wistar , Tumor Necrosis Factor-alpha/analysisABSTRACT
OBJECTIVE: To investigate the effects of using guided bone regeneration (GBR) technique with an bioresorbable collagen membrane for repairing bone defects around dental implants. METHODS: In 12 mongrel dogs, pure titanium implants were immediately implanted in mandibles after extraction of the mandibular third and fourth premolars of both sides, in which standard bone defects of 3 mm x 3 mm x 5 mm were created at mesial side of the implants. The defects on right side were covered with Co membrane, the left side was uncovered as control. The dogs were sacrificed 1, 2, 4, 6 months postoperatively. The specimens were removed and studied by gross observation, X-ray radiograph, histological examination, scanning electron microscope (SEM) and pull-out test. RESULTS: The quantity and quality of new bone formation in experimental side which bone defects covered with Co membrane were much better than that in the control side. CONCLUSION: With the biodegradable property and excellent biocompatibility, Co membrane can be used for guided bone regeneration to promote the bone repair progress, and the promotion acts mainly at early stage of bone healing.