Insight into the bacterial community composition of the plastisphere in diverse environments of a coastal salt marsh.

The microbial community colonized on microplastics (MPs), known as the 'plastisphere', has attracted extensive concern owing to its environmental implications. Coastal salt marshes, which are crucial ecological assets, are considered sinks for MPs. Despite their strong spatial heterogeneity, there is limited information on plastisphere across diverse environments in coastal salt marshes. Herein, a 1-year field experiment was conducted at three sites in the Yancheng salt marsh in China. This included two sites in the intertidal zone, bare flat (BF) and Spartina alterniflora vegetation area (SA), and one site in the supratidal zone, Phragmites australis vegetation area (PA). Petroleum-based MPs (polyethylene and expanded polystyrene) and bio-based MPs (polylactic acid and polybutylene succinate) were employed. The results revealed significant differences in bacterial community composition between the plastisphere and sediment at all three sites examined, and the species enriched in the plastisphere exhibited location-specific characteristics. Overall, the largest difference was observed at the SA site, whereas the smallest difference was observed at the BF site. Furthermore, the MP polymer types influenced the composition of the bacterial communities in the plastisphere, also exhibiting location-specific characteristics, with the most pronounced impact observed at the PA site and the least at the BF site. The polybutylene succinate plastisphere bacterial communities at the SA and PA sites were quite different from the plastispheres from the other three MP polymer types. Co-occurrence network analyses suggested that the bacterial community network in the BF plastisphere exhibited the highest complexity, whereas the network in the SA plastisphere showed relatively sparse interactions. Null model analyses underscored the predominant role of deterministic processes in shaping the assembly of plastisphere bacterial communities across all three sites, with a more pronounced influence observed in the intertidal zone than in the supratidal zone. This study enriches our understanding of the plastisphere in coastal salt marshes.

Effects of microplastics on the toxicity of co-existing pollutants to fish: A meta-analysis.

Aquatic ecosystems are among the main destination for microplastics (MPs) in the environment. MPs that enter aquatic ecosystems can contribute to pollution together with other co-existing pollutants. However, whether such pollution results in higher or lower toxicity to fish than that caused by co-existing pollutants alone remains controversial. This study aimed at closing this research gap based on 1380 biological endpoints under the background of environmental MP concentrations collected from 55 laboratory studies. Overall, MPs in co-existing pollutant solutions significantly increased the toxicity to fish. Specifically, MPs elevated negative effects on the immune system, metabolism, and oxidative damage. Subgroup analysis indicated that changes in toxicity were related to fish life stage and MP size, but not to co-existing pollutant or MP type. Meta-regression analysis indicated that changes in toxicity were not related to the logarithm of the octanol-water partition coefficient (logKow) or exposure time. Finally, the differences between laboratory research and the actual aquatic environment were discussed from four aspects: MPs, co-existing pollutants, environmental factors, and experimental objects. Our study provides a basis for further understanding the potential impact of MPs on aquatic organisms from a combined pollution perspective. Moreover, our results can provide a reference for the conservation and management of aquatic ecosystems.

Distribution of organophosphate ester fractions in sediment of the Eastern China Marginal Seas and the influencing factors.

Organic pollutant fractions should be closely investigated because of their different ecological risks. In this study, we examined the distribution of organophosphate ester (OPE) fractions (labile, stable-adsorbed, and tight-adsorbed fractions) in sediments from Eastern China Marginal Seas (ECMSs) and assessed the influencing factor of the fractions. The mean values of total OPEs in ECMSs are 13.70 ± 6.16 µg L-1 in seawater and 32.04 ± 14.31 µg kg-1 in sediment. The results showed that OPE concentration decreased from the northern to the southern ECMSs, and tris(1-chloro-2-propyl) phosphate and tris(1,3-dichloro-2-propyl) phosphate were the primary OPEs. The mean contents of labile, stable-adsorbed, and tight-adsorbed fractions in the ECMSs were 9.50, 11.29, and 11.71 µg kg-1, respectively. Labile OPEs were predominant in offshore waters; the percentage of stable- and tight-adsorbed fractions increased progressively with offshore distance in ECMSs. The specific surface area and surface functional groups of sediment were not consistent with the fraction concentrations from the correlation coefficient, but the gross domestic product per coastline agreed well with the fraction distribution. Based on this, we conclude that human activity, rather than physicochemical characteristics of sediments, may be the primary influencing factor of the relative distribution of different OPE concentration fractions in ECMSs.

Distribution pattern and influencing factors for the microplastics in continental shelf, slope, and deep-sea surface sediments from the South China Sea.

Marine microplastic pollution has become a major global concern in recent years and the fate of microplastics in the ocean is a hot issue of research. We investigated microplastic pollution in surface sediments in the northern South China Sea to explore its distribution characteristics and influencing factors across the continental shelf, continental slope, and deep-sea environments. It was found that the microplastic abundance of surface sediments was 130.56 ± 40.48 items/kg. The average abundance of microplastics in all three topographic areas gradually decreased with increasing distance offshore. However, the differences in microplastic diversity indices between the three areas were not significant and were higher than those in other seas of the world, indicating that the waters of the northern South China Sea are rich in microplastics from complex sources, with more pollution input channels. In the continental shelf, fibrous and low density microplastics accounted for the largest amount, with a low degree of microplastic aging, and were mostly transported by suspended-load. These microplastics were mainly influenced by human activities. In the deep sea, microplastics with higher density were the most abundant and the number of fibrous microplastics was fewer, while the average size was larger, mainly influenced by the bottom currents. These microplastics underwent long-term bedload transport. In the continental slope, the main factors affecting the distribution of microplastics were more complex. In addition to pollution by human activities, the slope also receives microplastic materials carried by bottom currents; therefore, the composition of microplastics in the slope combines those characteristics of microplastics in both the continental shelf and deep-sea areas. The findings of this study indicate that the South China Sea is affected by complex pollution sources under the dual effects of human activities and natural conditions; in particular, the pollution situation in the deep-sea area needs extensive attention.

Root exudates enhance the PAH degradation and degrading gene abundance in soils.

Root exudates could influence the bioavailability of polycyclic aromatic hydrocarbons (PAHs), provide nutrients for soil microorganisms, and affect PAH biodegradation. However, it remains unclear how a bacterial community and its PAH-degrading genes play crucial roles in PAH biodegradation and respond to root exudates. In this study, a 32-day soil microcosm study was conducted to explore the impacts of artificial and actual root exudates on PAH degradation, degrading genes, and bacterial community structure. The results showed that 10-100 mg DOC/kg artificial and actual root exudates promoted the degradation of naphthalene, phenanthrene, and pyrene in soils, and their percent removal increased initially and then decreased with the increasing root exudates. Quantitative polymerase chain reaction analysis and 16S rRNA gene high-throughput sequencing suggested that the artificial root exudates significantly promoted the Nocardioides and Arthrobacter genera, which may harbor the nidA gene (the representative PAH-degrading gene from Gram-positive bacteria). In contrast, actual root exudates significantly stimulated the Pseudomonas genus that may harbor the nahAc gene (the representative PAH-degrading gene from Gram-negative bacteria). The correlation analysis further indicated that the absolute abundance of PAH degraders and degrading genes had strong correlations with PAH degradation efficiency. Therefore, these findings suggest that root exudates enhanced PAH biodegradation probably due to increases in abundance of both PAH-degraders and their degrading genes.

Chlorpyrifos inhibits nitrogen fixation in rice-vegetated soil containing Pseudomonas stutzeri A1501.

Chlorpyrifos, a common organophosphorus pesticide, is widely used for agricultural pest control and can inhibit nitrogen-fixing bacteria biomass in paddy. In this study, the additions of chlorpyrifos (1 and 8 mg kg-1) to soil, with or without Pseudomonas stutzeri A1501, resulted in a significant decrease in nitrogen fixation, despite insignificant effects on the abundances of P. stutzeri A1501 and bacteria in soil. Toxic effect of chlorpyrifos on P. stutzeri A1501 nitrogenase activity in medium was also observed, accompanied by a significant reduction in the expression of nitrogen-fixing related genes (nifA and nifH). Furthermore, rhizosphere colonization and biofilm formation by P. stutzeri A1501 were repressed by chlorpyrifos, leading to decreased nitrogenase activity in the rhizosphere. Biofilm formation in medium was inhibited by bacterial hyperkinesis and reduction of extracellular polymeric substance, including exopolysaccharides and proteins. Together, these findings showed that chlorpyrifos-induced production of reactive oxygen species (ROS) which was directly responsible for reduced nitrogenase activity in the medium, soil, and rhizosphere by inhibiting the expressions of nitrogen-fixing related genes. Furthermore, the inhibition of biofilm formation by chlorpyrifos or ROS likely aggravated the reduction in rhizospherere nitrogenase activity. These findings provide potentially valuable insights into the toxicity of chlorpyrifos on nitrogen-fixing bacteria and its mechanisms. Furthermore, for sustainable rice production, it is necessary to evaluate whether other pesticides affect nitrogen fixation and select pesticides that do not inhibit nitrogen fixation.

Antibiotics in animal manure and manure-based fertilizers: Occurrence and ecological risk assessment.

The extensive use of antibiotics globally and their residues in the environment has become a serious concern. Intensive animal farming is considered to be a major contributor to the increased environmental burden of antibiotics. Although some antibiotic investigations have been advancing around the world, as an important agricultural country, the information on these pollutants in animal farms are very limited in China. Previous studies have explored few antibiotic residues in livestock farms, whereas information on some antibiotics has remained unknown. The current study analyzed residues of 32 common veterinary antibiotics in manure and manure-based fertilizers collected from Jiangsu Province, China. In most of the manure and fertilizer samples, sulfamethazine and tetracycline were present, with high concentration up to 5650 and 1920 µg·kg-1, respectively. These detected antibiotics have weak relationships with physicochemical properties. Ciprofloxacin, enrofloxacin, sulfamethazine, and sulfachlorpyridazine, hence pose a high potential risk to crops based on the toxicological data of organisms and plants in the soil environment. However, soil invertebrate, such as earthworms, Planococcus Citri. and Folsomia fimeraria., had low ecological risks. Our results showed the presence of antibiotics in livestock and poultry farms plus the potential risks to the soil ecosystem. Therefore, the findings can provide guidelines for monitoring antibiotic residues in agroecosystems, as well as insights into the associated ecological risks of using the two products.