[The study of ovarian autoimmunity and ovarian reserve in women with uncertain sterility].

UNLABELLED: Ovarian autoimmunity can be induce ovarian function, which is shown clinically with premature ovarian failure, unknown sterility and unsuccessful in vitro fertilizations. Antiovarian antibodies are directed to antigens functional zones of zona pellucida and granulose cells with important part in follicle genesis and fertilization. AIM: We have to investigate the influence of ovarian autoimmunity, antiovarian and antizona pellucida antibodies on endocrinology markers of ovarian reserve: inhibin B, FSH, LH by the women with unknown sterility. MATERIALS AND METHODS: 340 women were investigated with unknown sterility and 60 women were investigated with only one successful pregnancy at least. We investigated them for presence of antiovarian antibodies, antizona pellucida antibodies, and of course the levels of FSH, LH, inhibin B on the third day of menstrual cycle. Serological methods were used: ELISA- biochemical technique to detect the presence of antiovarian antibodies and antizona pellucida antibodies, inhibin B, FSH, LH. Statistical methods for analysis were also used. RESULTS: In investigated group was found the presence of antiovarian antibodies in 58 women (17.05%) and in control group was not found. Antizona pellucida antibodies presence was found in 43 women (12.64%) in investigated group and in control found was not found. It was not found important difference in levels of FSH, LH, inhibin B on the third day of menstrual cycle between women with and women without antiovarian antibodies. CONCLUSION: Antiovarian antibodies were observed only in women who have had laparoscopic ovarian drilling. The frequency of antiovarian antibodies among the Bulgarian women with unknown sterility is 17.05%. The presence of antiovarian antibodies is not associated with poor ovarian reserve as we investigated the levels of FSH, LH, inhibin B on the third day of the menstrual cycle.

[The importance of the presence of antisperm antibodies in serum and ejaculate of men with infertility].

UNLABELLED: There are multiple insults to the male genital tract that have been associated with an increased risk of antisperm antibodies (ASA) formation. The presence of ASA has been regarded as typical and specific for the immunological infertility. It has been suggested that the presence of agglutinated spermatozoa is suggestive of the existence of an immunological cause of infertility such as the existence of ASA. AIM: The objective of this study was to determine the clinical significance of serum and seminal plasma ASA as well as whether varying degrees of sperm agglutination can be a predictive indicator of positive serum and/or seminal plasma ASA. PATIENTS AND METHODS: 100 infertile and 30 fertile men were tested for ASA in seminal plasma and serum. The evaluation of patients included complete history, physical examination, scrotal ultrasound and semen analysis. ASA in serum and seminal plasma were tested by ELISA (Biosource, Belgium) and results were compared with the data of the semen analysis. RESULTS: 44 (44%) of the patients tested positive for ASA in seminal plasma, 9 of them had positive serum ASA. In the control group, seminal plasma ASA were not detected and two men (6.7%) tested positive for serum ASA. Correlation was established between seminal plasma ASA and two of the semen analysis markers: agglutination and increased viscosity. CONCLUSION: ASA in seminal plasma are much more predictive than ASA in serum and have major role in the pathogenesis and diagnostics of male infertility.