ABSTRACT
Enterotoxemia, a disease that affects domestic ruminants, is caused mainly by the epsilon toxin from Clostridium perfringens type D. Its eradication is virtually impossible, control and prophylaxis are based on systematic vaccination of herds with epsilon toxoids that are efficient in inducing protective antibody production. The use of recombinant toxins is one of the most promising of these strategies. This work evaluates the potency of a Cl. perfringens type D epsilon toxoid expressed by Escherichia coli administered to goats, sheep, and cattle. The etx gene was cloned into the pET-11a plasmid of E. coli strain BL21 to produce the recombinant toxin. Rabbits (n=8), goats, sheep, and cattle (n=5 for each species) were immunized with 0.2mg of the insoluble recombinant protein fraction to evaluate vaccine potency of the epsilon toxoid studied. Antibody titers were 40, 14.3, 26, and 13.1 IU/mL in the rabbit, goat, sheep, and cattle serum pools, respectively. The epsilon toxoid produced and tested in this work is adequate for immunization of ruminants against enterotoxemia.
Subject(s)
Bacterial Toxins/immunology , Bacterial Vaccines/immunology , Enterotoxemia/prevention & control , Animals , Antibodies, Bacterial/blood , Antibodies, Neutralizing/blood , Bacterial Toxins/genetics , Cattle , Cattle Diseases/immunology , Cattle Diseases/prevention & control , Enterotoxemia/immunology , Escherichia coli/genetics , Escherichia coli/metabolism , Goat Diseases/immunology , Goat Diseases/prevention & control , Goats , Rabbits , Recombinant Proteins/immunology , Sheep , Sheep Diseases/immunology , Sheep Diseases/prevention & controlABSTRACT
Este trabalho teve como objetivo avaliar a viabilidade de um isolado de Tritrichomonas foetus criopreservada com glicerol e dimetilsulfóxido (DMSO) a -196ºC. Isolados do protozoário foram descongeladas dois dias após o congelamento e 6, 12, 18 e 26 meses, para avaliação de sua viabilidade. Em todos os tempos analisados, o congelamento foi viável em 60 por cento e 90 por cento das amostras congeladas com glicerol a 10 por cento e DMSO a 12 por cento, respectivamente.
The aim of this work was to evaluate the viabilitity of Tritrichomonas foetus cryopreservated with glycerol and dimethilsulphosyde (DMSO) at -196ºC. Samples of the protozoa were thaw two days and 6, 12, 18, and 26 months after freezing to be evaluated. In the time analyzed, the freezing was viable in 60 percent and 90 percent of freeze samples with glycerol at 10 percent and DMSO at 12 percent, respectively.
Subject(s)
Animals , Cryopreservation/methods , Tritrichomonas foetus , Time FactorsABSTRACT
The aim of this work was to evaluate the viabilitity of Tritrichomonas foetus cryopreservated with glycerol and dimethilsulphosyde (DMSO) at -196 degrees C. Samples of the protozoa were thaw two days and 6, 12, 18, and 26 months after freezing to be evaluated. In the time analyzed, the freezing was viable in 60% and 90% of freeze samples with glycerol at 10% and DMSO at 12%, respectively.
