ABSTRACT
Rhabditis spp., is a nematode known to cause otitis externa, an infection difficult to control, in cattle reared within tropical regions. The objective of this study was to assess the combined use of ivermectin 1%, dimethyl sulfoxide 1% and mineral oil 100% containing nematophagous fungi of both Duddingtonia flagrans (AC001) and Monacrosporium thaumasium (NF34) species to control in vitro Rhabditis spp. Thus, 12 experimental groups were designed with eight replicates each: G1 (nematodesâ¯+â¯AC001); G2 (nematodesâ¯+â¯NF34); G3 (nematodesâ¯+â¯ivermectin 1%/positive control); G4 (nematodesâ¯+â¯dimethyl sulfoxide 1%/positive control); G5 (nematodesâ¯+â¯mineral oil 100%/positive control); G6 (nematodesâ¯+â¯AC001 + ivermectin 1%); G7 (nematodesâ¯+â¯NF34 + ivermectin 1%); G8 (nematodesâ¯+â¯AC001 + mineral oil 100%); G9 (nematodesâ¯+â¯NF34 + mineral oil 100%); G10 (nematodesâ¯+â¯AC001 + dimethyl sulfoxide 1%); G11 (nematodeâ¯+â¯NF34 + dimethyl sulfoxide 1%); G12 (nematodeâ¯+â¯distilled water/negative control). The results demonstrated that all experimentally treated groups differed statistically (pâ¯<â¯0.01) from the control group. In the present study, the use of dimethyl sulfoxide 1% and mineral oil 100% in conjunction with conidia fungi portrayed noteworthy outcomes, which represents a future premise for the combined use of nematophagous fungi within these vehicles in both controlling Rhabditis spp.
