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Arch Virol ; 157(9): 1821-4, 2012 Sep.
Article in English | MEDLINE | ID: mdl-22622432

ABSTRACT

The complete genome sequence of a Chinese narcissus isolate of narcissus late season yellows virus from Zhangzhou, China (NLSYV-ZZ), was determined to be 9,651 nucleotides in length, excluding the 3'-terminal poly (A) tail, by amplification and sequencing of virus RNA. The viral genome contains a single long open reading frame of 9,315 nucleotides encoding a polyprotein of 3,105 amino acids. The polyprotein was predicted to be cleaved into ten mature proteins by three viral proteases. Complete genome sequence comparison and phylogenetic analysis indicated that NLSYV-ZZ was most closely related to narcissus yellow stripe virus (NYSV), which was also isolated from narcissus. These viruses shared 69.9 % identity in their complete nucleotide sequences and 77.0 % identity in their polyprotein amino acid sequences.


Subject(s)
Genome, Viral , Narcissus/virology , Plant Diseases/virology , Potyvirus/genetics , RNA, Viral/genetics , Sequence Analysis, DNA , China , Cluster Analysis , Molecular Sequence Data , Open Reading Frames , Phylogeny , Polyproteins/genetics , Potyvirus/isolation & purification , Sequence Homology, Amino Acid , Sequence Homology, Nucleic Acid , Viral Proteins/genetics
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