ABSTRACT
BACKGROUND: The predictability and concordance between simulated and actual outcomes in rhinoplasty are uncertain. Here, we introduce a suture positioning technique (SPT), a simple and low-cost method to minimize the gap between the simulated and actual outcomes of rhinoplasty. METHODS: Seventy patients were enrolled in this study between January 2018 and January 2021. Preoperative simulations were performed using Adobe Photoshop. The control group underwent surgery using simulation and intuition. In the SPT group, sutures were used to assist in the preoperative identification of the ideal nasal tip position. The SPT effectiveness was tested by measuring the nasal parameters and using the patient's subjective satisfaction questionnaire at T1 (Time 1, immediately postoperatively) and T2 (Time 2, at least 1 year postoperatively). RESULTS: The intraclass correlation coefficient test showed a satisfactory correlation between simulation and postoperative outcomes in both groups. However, the SPT group had a higher correlation than the control group, especially for the nasal length (16% higher at T1 and 15% higher at T2). The mean absolute difference (MAD) between the outcomes and simulation indicated that the MAD of nasal tip projection between T2 and simulation and MAD of nasal length between T1 (or T2) and simulation were statistically significant between groups. Additionally, the SPT group was more satisfied with the postoperative outcomes and were consistent with the preoperative simulation. CONCLUSION: This study demonstrated the effectiveness of SPT in intraoperative quality control. This technique may be adopted by surgeons to achieve good concordance between simulated and actual surgical outcomes.
Subject(s)
Rhinoplasty , Humans , Rhinoplasty/methods , Treatment Outcome , Esthetics , Nose/surgery , Sutures , Nasal Septum/surgeryABSTRACT
Selective separation and recovery of Co(II)/Mn(II) from purified terephthalic acid (PTA) production wastewater is very important to reduce the Co(II)/Mn(II) catalysts consumption and control the pollutant discharge. This work employed zeolites NaA, NaX, and HZSM-5 with different pore sizes and Na(I) contents to selectively separate and recover Co(II)/Mn(II) from PTA wastewater and to understand the adsorption mechanism. It is found that only NaA can exclusively adsorb Co(II)/Mn(II) through ion-exchange without adsorbing any aromatic organic compound (AOC); oppositely, HZSM-5 shows the highest adsorption capacity for AOCs but almost no adsorption for Co(II)/Mn(II); and NaX exhibits moderate adsorption capacities for both Co(II)/Mn(II) and AOCs. Moreover, pH can significantly impact the adsorption of both Co(II)/Mn(II) and AOCs due to the competitive adsorption between H(I) and Co(II)/Mn(II) and the electrostatic repulsion between AOCs and zeolites. The adsorption kinetics, isotherms, and thermodynamics were also investigated to lay a good basis for process development. Importantly, bench-scale experiments for simulating the industrial operation were carried out, and the results show that the adsorption capacity of the NaA particles for Co(II)/Mn(II) from the industrial PTA wastewater is 9.1/8.6â¯mg/g, respectively, without adsorbing any AOC. Therefore, an efficient strategy to selectively separate and recover Co(II)/Mn(II) from PTA wastewater was successfully developed.
ABSTRACT
Barnyardgrass (Echinochloa crus-galli) is a pernicious weed in agricultural fields worldwide. The molecular mechanisms underlying its success in the absence of human intervention are presently unknown. Here we report a draft genome sequence of the hexaploid species E. crus-galli, i.e., a 1.27 Gb assembly representing 90.7% of the predicted genome size. An extremely large repertoire of genes encoding cytochrome P450 monooxygenases and glutathione S-transferases associated with detoxification are found. Two gene clusters involved in the biosynthesis of an allelochemical 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA) and a phytoalexin momilactone A are found in the E. crus-galli genome, respectively. The allelochemical DIMBOA gene cluster is activated in response to co-cultivation with rice, while the phytoalexin momilactone A gene cluster specifically to infection by pathogenic Pyricularia oryzae. Our results provide a new understanding of the molecular mechanisms underlying the extreme adaptation of the weed.
Subject(s)
Echinochloa/physiology , Genome, Plant , Plant Weeds/physiology , Adaptation, Physiological , Echinochloa/genetics , Echinochloa/growth & development , Genome Size , Oryza/growth & development , Pheromones/metabolism , Plant Proteins/genetics , Plant Weeds/genetics , Plant Weeds/growth & developmentABSTRACT
The genus Echinochloa (Poaceae) includes numerous problematic weeds that cause the reduction of crop yield worldwide. To date, DNA sequence information is still limited in the genus Echinochloa. In this study, we completed the entire chloroplast genomes of two Echinochloa species (Echinochloa oryzicola and Echinochloa crus-galli) based on high-throughput sequencing data from their fresh green leaves. The two Echinochloa chloroplast genomes are 139,891 and 139,800 base pairs in length, respectively, and contain 131 protein-coding genes, 79 indels and 466 substitutions helpful for discrimination of the two species. The divergence between the genus Echinochloa and Panicum occurred about 21.6 million years ago, whereas the divergence between E. oryzicola and E. crus-galli chloroplast genes occurred about 3.3 million years ago. The two reported Echinochloa chloroplast genome sequences contribute to better understanding of the diversification of this genus.
Subject(s)
Echinochloa/genetics , Genome, Chloroplast , Base Sequence , DNA, Plant/genetics , Echinochloa/classification , Evolution, Molecular , INDEL Mutation , Phylogeny , Plant Leaves/genetics , Sequence AlignmentABSTRACT
MAIN CONCLUSION: Whole-genome re-sequencing of weedy rice from southern China reveals that weedy rice can originate from hybridization of domesticated indica and japonica rice. Weedy rice (Oryza sativa f. spontanea Rosh.), which harbors phenotypes of both wild and domesticated rice, has become one of the most notorious weeds in rice fields worldwide. While its formation is poorly understood, massive amounts of rice genomic data may provide new insights into this issue. In this study, we determined genomes of three weedy rice samples from the lower Yangtze region, China, and investigated their phylogenetics, population structure and chromosomal admixture patterns. The phylogenetic tree and principle component analysis based on 46,005 SNPs with 126 other Oryza accessions suggested that the three weedy rice accessions were intermediate between japonica and indica rice. An ancestry inference study further demonstrated that weedy rice had two dominant genomic components (temperate japonica and indica). This strongly suggests that weedy rice originated from indica-japonica hybridization. Furthermore, 22,443 novel fixed single nucleotide polymorphisms were detected in the weedy genomes and could have been generated after indica-japonica hybridization for environmental adaptation.
Subject(s)
Agriculture , Genome, Plant , Hybridization, Genetic , Oryza/genetics , Plant Weeds/genetics , Sequence Analysis, DNA , Alleles , China , Chromosome Mapping , Chromosomes, Plant/genetics , Genes, Plant , Genetics, Population , Phenotype , Polymorphism, GeneticABSTRACT
The present paper reports photocatalytic degradation of bovine serum albumin (BSA) by TiO2 under near UV (365 nm) irradiation. Ultraviolet-Visible spectrometry, FTIR spectrometry and HPLC were used to study the degradation and characterize the final degradation products. It was found that under near UV irradiation, BSA (0.1 g x L(-1)) can be totally degraded to small fragments by TiQ2 in 5 h. Total mineralization of BSA (93.5%) is reached after reacting for 40 h. C, N and S in BSA are mineralized to give CO2, NO3- and SO4(2-). It is suggested that the degradation of BSA happens in two steps: first to small fragments, then further mineralized to small inorganic molecule. The absence of UV irradiation or TiQ2 can only lead to partial degradation of BSA (30%) in 5 h and no CO2 is observed even in longer reaction time.