The imbalance of pulmonary Th17/Treg cells in BALB/c suckling mice infected with respiratory syncytial virus-mediated intestinal immune damage and gut microbiota changes.

The immune response induced by respiratory syncytial virus (RSV) infection is closely related to changes in the composition and function of gastrointestinal microorganisms. However, the specific mechanism remains unknown and the pulmonary-intestinal axis deserves further study. In this study, the mRNA levels of ROR-γt and Foxp3 in the lung and intestine increased first and then decreased. IL-17 and IL-22 reached the maximum on the third day after infection in the lung, and on the second day after infection in the small intestine and colon, respectively. RegⅢγ in intestinal tissue reached the maximum on the third day after RSV infection. Moreover, the genus enriched in the RSV group was Aggregatibacter, and Proteus was reduced. RSV infection not only causes Th17/Treg cell imbalance in the lungs of mice but also leads to the release of excessive IL-22 from the lungs through blood circulation which binds to IL-22 receptors on the intestinal surface, inducing RegⅢγ overexpression, impaired intestinal Th17/Treg development, and altered gut microbiota composition. Our research reveals a significant link between the pulmonary and intestinal axis after RSV infection. IMPORTANCE: RSV is the most common pathogen causing acute lower respiratory tract infections in infants and young children, but the complex interactions between the immune system and gut microbiota induced by RSV infection still requires further research. In this study, it was suggested that RSV infection in 7-day-old BALB/c suckling mice caused lung inflammation and disruption of Th17/Treg cells development, and altered the composition of gut microbiota through IL-22 induced overexpression of RegⅢγ, leading to intestinal immune injury and disruption of gut microbiota. This research reveals that IL-22 may be the link between the lung and gut. This study may provide a new insight into the intestinal symptoms caused by RSV and other respiratory viruses and the connection between the lung and gut axis, as well as new therapeutic ideas for the treatment of RSV-infected children.

Expectancy-Value Motivation and Physical Activity- and Health-Related Outcomes among At-Risk Children and Adolescents.

Despite a large amount of research having been done to examine and promote physical activity and health among adolescents and children, relatively little attention has been paid attention to underrepresented populations. In this study, we investigated the relationships between expectancy-value motivation and physical activity- and health-related outcomes among a group of at-risk boys at a summer sports camp. The total participants included 107 boys (Mage = 11.78 years, SD = 1.20). The boys' perceived expectancy beliefs (EXP), importance (IMP), interest (INT), usefulness (USE), effort (EFT), and intention for future participation (IFP) were assessed using established questions on a five-point Likert scale, and a PACER test was performed to estimate their cardiovascular fitness (CVF). Through a path analysis, we found that EXP positively predicted CVF (ß = 0.19, p < 0.01), IMP positively predicted EFT (ß = 0.26, p < 0.01), and INT positively predicted both EFT (ß = 0.34, p < 0.01) and IFP (ß = 0.28, p < 0.01), while USE had no statistically significant effect on either EFT, IFP, or CVF. We discussed the limitations and implications of the present study. We recommend including a diverse sample and employing the expectancy-value model in future research, and advocating expectancy beliefs and task values, especially importance and interest, among participants during physical activity promotion.

Global Accelerometer-derived Physical Activity Levels from Preschoolers to Adolescents: A Multilevel Meta-analysis and Meta-regression.

BACKGROUND: Global level physical activity surveillance studies were primarily based on self-report data that could generate inaccurate results. PURPOSE: To investigate global accelerometer-measured daily moderate to vigorous physical activity (MVPA) changes from preschool age to adolescence as well as gender differences in MVPA while adjusting for the geographic regions and major MVPA cut points. METHODS: A comprehensive search was conducted through August 2020 that includes 30 databases such as Academic Search Ultimate, Child Development & Adolescent Studies, Education Full Text, ERIC, General Science, PsycINFO, ScienceDirect, and SPORTDiscuss. We included both cross-sectional and longitudinal MVPA tracking studies with daily MVPA being measured by waist-worn accelerometers and determined by either Freedson 3 METs, 4 METs, or Evenson cut points for preschoolers, children, and adolescents. RESULTS: Researchers analyzed 84 studies reporting on 124 effect sizes with a total of 57,587 participants. The combined data showed significant MVPA differences among various continents of participants (p < .001) or cut points (p < .05-.001) for both preschoolers, children, and adolescents. Globally, when continents and cut points were controlled, individuals' daily MVPA time decreased every year by an average of 7.88, 10.37, and 6.68 min from preschool age to adolescence, preschool age to children, and children to adolescence, respectively. When cut points and continents were controlled, boys had significantly higher daily MVPA than girls for all three age groups (p < .001). CONCLUSIONS: Globally, individuals' daily MVPA starts to decline dramatically as early as the beginning of preschool age. Early intervention is needed to counteract the high decline rate in MVPA.

Metabolomics-based investigation of SARS-CoV-2 vaccination (Sinovac) reveals an immune-dependent metabolite biomarker.

SARS-CoV-2 and its mutant strains continue to rapidly spread with high infection and fatality. Large-scale SARS-CoV-2 vaccination provides an important guarantee for effective resistance to existing or mutated SARS-CoV-2 virus infection. However, whether the host metabolite levels respond to SARS-CoV-2 vaccine-influenced host immunity remains unclear. To help delineate the serum metabolome profile of SARS-CoV-2 vaccinated volunteers and determine that the metabolites tightly respond to host immune antibodies and cytokines, in this study, a total of 59 sera samples were collected from 30 individuals before SARS-CoV-2 vaccination and from 29 COVID-19 vaccines 2 weeks after the two-dose vaccination. Next, untargeted metabolomics was performed and a distinct metabolic composition was revealed between the pre-vaccination (VB) group and two-dose vaccination (SV) group by partial least squares-discriminant and principal component analyses. Based on the criteria: FDR < 0.05, absolute log2 fold change greater than 0.25, and VIP >1, we found that L-glutamic acid, gamma-aminobutyric acid (GABA), succinic acid, and taurine showed increasing trends from SV to VB. Furthermore, SV-associated metabolites were mainly annotated to butanoate metabolism and glutamate metabolism pathways. Moreover, two metabolite biomarkers classified SV from VB individuals with an area under the curve (AUC) of 0.96. Correlation analysis identified a positive association between four metabolites enriched in glutamate metabolism and serum antibodies in relation to IgG, IgM, and IgA. These results suggest that the contents of gamma-aminobutyric acid and indole in serum could be applied as biomarkers in distinguishing vaccinated volunteers from the unvaccinated. What's more, metabolites such as GABA and taurine may serve as a metabolic target for adjuvant vaccines to boost the ability of the individuals to improve immunity.

The 3 × 2 achievement goal model and its relation to students' persistence/effort in college physical activity classes.

Objective: This study examined (1) the validity and reliability of the 3 × 2 achievement goal questionnaire (3 × 2 AGQ) in a college physical activity (PA) setting, and (2) relationships between achievement goals and students' persistence/effort. Methods: Participants were 556 students (M = 20.31 years, SD = 1.34; 305 males; 251 females) enrolled in PA classes at a major university in the southwest U.S. Results: Results indicated that the 3 × 2 AGQ failed to assess six achievement goals as construed in the 3 × 2 model of achievement goals in academic settings. However, it served as a reliable and valid measure assessing task/self-approach, task/self-avoidance, other-approach, and other-avoidance goals in a college PA setting. All except other-avoidance goals were found motivationally beneficial and positively predicted persistence/effort. Conclusions: Instructors are encouraged to figure out how to use their influences and peers' influences to help students endorse achievement goals that contribute to positive motivation, such as task/self-approach goals.

Longitudinal Stability and Change in Young Students' Mastery-Approach Goals for Running.

In the present study, we examined the longitudinal stability and change in mastery-approach goals for running as offered in regularly scheduled physical education (PE) and athletics classes in the United States. There were five waves of data collection from 806 students (431 boys; 375 girls) who were tracked from fourth to eighth grade while participating in running activities in PE or athletics classes. We assessed the participants' mastery-approach goals using four items on a 5-point scale. We found acceptable longitudinal construct validity, measurement invariance, and scale reliability for the scores of mastery-approach goals, and we found these goals to have moderate stability across this 5-year period. Latent growth modeling revealed a linear decline in mean mastery-approach goal scores over the five school years. We concluded that the assessment of mastery-approach goals over time was both valid and reliable and that these running mastery-approach goals were stable in most respects but became less intense over time.

Downregulated NLRP3 and NLRP1 inflammasomes signaling pathways in the development and progression of type 1 diabetes mellitus.

BACKGROUND: NLRP3 and NLRP1 inflammasomes signaling pathways may play an important role in the development and progression of type 1 diabetes mellitus (T1DM). However, relative research is rare. METHODS: The blood mononuclear cells (PBMCs) and granulocytes (GCs) were collected from T1DM patients. The mRNA and protein expression levels were detected by qRT-PCR and Western blotting, respectively. The NLRP3/NLRP1was knocked down by transfected with siRNA, or ovexpressed by infected with lentiviral vectors in PBMCs and GCs from non obese diabetic (NOD) mice, respectively. The occurrence of diabetes was evaluated and the intraperitoneal glucose tolerance was tested in NOD mice with IL-1 receptor-associated kinase-M deficiency (IRAK-M-/-). The pancreas and lymphonodus of IRAK-M-/- NOD mice were also collected for detection of NLRP3/NLRP1 expression. RESULTS: NLRP3, NLRP1, Caspase-1and IL-1ß were significantly downregulated in the PBMCs and GCs of patients with T1DM, and NLRP3 and NLRP1 were markedly downregulated in T1DM patients with DKA compared to that with ND and CC. Further study indicated that IL-1ß mRNA expression level was positively correlated with the expression levels of NLRP3, NLRP1 and Caspase-1. Besides, NLRP3/NLRP1 knockdown decreased the expression levels of Caspase-1 and IL-1ß; whereas NLRP3/NLRP1 overexpression increased the expression levels of Caspase-1 and IL-1ß in vitro. IRAK-M-/- NOD mice had early onset and rapid progression of T1DM, and weak glucose tolerance, which was regarded as an early T1D mouse model. The mRNA expression levels of NLRP3, NLRP1, Caspase-1and IL-1ß in the pancreas and lymphonodus of IRAK-M-/- NOD mice were significantly higher compared to that of IRAK-M+/+ NOD mice. CONCLUSION: NLRP3 and NLRP1 inflammasomes signaling pathways were associated with the development and progression of T1DM, which response as protective factors in the early stage of T1DM.

Procalcitonin and C-reactive protein in urinary tract infection diagnosis.

BACKGROUND: Urinary infections are a common type of pediatric disease, and their treatment and prognosis are closely correlated with infection location. Common clinical manifestations and laboratory tests are insufficient to differentiate between acute pyelonephritis and lower urinary tract infection. This study was conducted to explore a diagnostic method for upper and lower urinary tract infection differentiation. METHODS: The diagnostic values of procalcitonin (PCT) and C-reactive protein (CRP) were analyzed using the receiver operating characteristic curve method for upper and lower urinary tract infection differentiation. PCT was determined using chemiluminescent immunoassay. RESULTS: The PCT and CRP values in children with acute pyelonephritis were significantly higher than those in children with lower urinary tract infection (3.90 ± 3.51 ng/ml and 68.17 ± 39.42 mg/l vs. 0.48 ± 0.39 ng/ml and 21.39 ± 14.92 mg/l). The PCT values were correlated with the degree of renal involvement, whereas the CRP values failed to show such a significant correlation. PCT had a sensitivity of 90.47% and a specificity of 88% in predicting nephropathia, whereas CRP had sensitivity of 85.71% and a specificity of 48%. CONCLUSIONS: Both PCT and CRP can be used for upper and lower urinary tract infection differentiation, but PCT has higher sensitivity and specificity in predicting pyelonephritis than CRP. PCT showed better results than CRP. PCT values were also correlated with the degree of renal involvement.