Inter-software and inter-threshold reliability of quantitative paraspinal muscle segmentation.

PURPOSE: Changes in the cross-sectional area (CSA) and functional cross-sectional area (FCSA) of the lumbar multifidus (MF) and erector spinae muscles (ES) are factors that can contribute to low back pain. For the assessment of muscle CSA and composition there are various software and threshold methods used for tissue segmentation in quantitative analysis. However, there is currently no gold standard for software as well as muscle segmentation. This study aims to analyze the measurement error between different image processing software and different threshold methods for muscle segmentation. METHODS: Magnetic resonance images (MRI) of 60 patients were evaluated. Muscle CSA and FCSA measurements were acquired from axial T2-weighted MRI of the MF and ES at L4/L5 and L5/S1. CSA, FCSA, and FCSA/CSA ratio were measured independently by two observers. The MRI images were measured using two different software programs (ImageJ and Amira) and with two threshold methods (Circle/Overlap method) for each software to evaluate FCSA and FCSA/CSA ratio. RESULTS: Inter-software comparisons revealed high inter-rater reliability. However, poor inter-rater reliability were obtained with different threshold methods. CSA, FCSA, and FCSA/CSA showed excellent inter-software agreement of 0.75-0.99 regardless of the threshold segmentation method. The inter-rater reliability between the two observers ranged between 0.75 and 0.99. Comparison of the two segmentation methods revealed agreement between 0.19 and 0.84. FCSA and FCSA/CSA measured via the Overlap method were significantly higher than those measured via the Circle method (P < 0.01). CONCLUSION: The present study showed a high degree of reliability with very good agreement between the two software programs. However, study results based on different threshold methods should not be directly compared.

Seasonal distribution of water masses and their impacts on nutrient supply in the southern Beibu Gulf.

Dual water isotopes were investigated to reveal the seasonal distribution of water masses and their impacts on nutrient supply in southern Beibu Gulf. In summer and winter, the South China Sea (SCS) water (61-69%) contributed the most to the seawater in the southern Beibu Gulf, followed by the diluted water (24-34%), and the west-Guangdong coastal current (WGCC) (5-7%) had the minimum contribution. However, the major nutrient source shifted from the diluted water in summer (39-73%) to the SCS water (57-90%) in winter. The WGCC's impact on nutrient loads was relatively small (2-10% in summer, 4-34% in winter). Our results highlight the control of nutrient supply was the SCS water (winter) and diluted water (summer), with limited influence from the WGCC, providing new insights into the impact of water mass transportation and its nutrient supply in the Beibu Gulf.

A review of the marine biogeochemical response to typhoons.

Typhoons are extreme weather events that can not only affect marine dynamics, but also change marine biogeochemistry, considerably impacting the climate. Based on the satellite remote sensing data, the upwelling of abundant nutrients induced by typhoons from deeper eutrophic water to the upper oligotrophic layer triggers phytoplankton blooms in the upper oceans, thereby increasing new productivity (as a carbon sink). However, field observations have shown that organic matter decomposition (as a carbon source) is the dominant process regardless of whether phytoplankton blooms occur after typhoons, resulting in oxygen consumption in the water column. Therefore, it is particularly important to comprehensively study the coupling mechanisms of biogeochemistry and dynamics in the ocean after typhoons. Here, we present a systematic overview summarizing the effects of typhoons on marine dynamics and biogeochemistry and elaborating on the characteristics and mechanisms of organic matter decomposition induced by typhoons.

Funeral arrangements requested by suicide decedents in Shanghai and Wuhan, China.

This qualitative study analyzed suicide notes left by 591 decedents in Shanghai and Wuhan, in China. General guidelines and detailed requests with regard to funeral service were coded. In general, suicide decedents preferred untraditional funerals, which were simple and speedy rather than grand and costly funeral arrangements. These choices suggested that suicide decedents could fear stigmatization and discrimination. Some suicide decedents regarded suicides as vicious deaths, and thus wished their families to dispose of the cursed bodily remains and tombs as swiftly as possible.

Self-Stigma and Its Relationship With Rates of Depression Diagnosis.

Background: Self-stigma about suicide might impede people seeking help from mental health professionals. There is little research about self-stigma expressed by suicide decedents. Aims: We aimed to explore (a) self-stigma about suicide through examination of suicide notes; and (b) whether the expression of self-stigma was associated with the formal diagnoses of depression. Method: Data were extracted from notes left by people who died by suicide in two major Chinese cities (Shanghai, 2004-2017; Wuhan, 2005-2019). Note content was examined and self-stigma items were coded. Demographics associated with self-stigma were reported. Rates of depression were compared between note-leavers who expressed self-stigma, and those who did not. Results: Notes were left by 567 suicide decedents (representing about 19% all suicides). Approximately 25% notes contained at least one self-stigma item. Older people made fewer self-stigma references, as did people from Wuhan. Depression was not associated with self-stigma. Limitations: Not all people dying from suicide leave notes, and suicide notes variably report self-stigma, thus self-stigma about suicide may be underestimated. Conclusion: Self-stigma items varied across regions and age groups, but not with depression. Therefore, self-stigma expressed by suicide decedents may not reflect help-seeking behaviours from professional mental health services.

Plant growth, salt removal capacity, and forage nutritive value of the annual euhalophyte Suaeda salsa irrigated with saline water.

Sustainable agricultural development in semiarid and arid regions is severely restricted by soil and water salinization. Cultivation of the representative halophyte Suaeda salsa, which can be irrigated with saline water and cultivated on saline soils, is considered to be a potential solution to the issues of freshwater scarcity, soil salinization, and fodder shortage. However, the salt removal capacity and differences in the forage nutritive value of S. salsa under different saline water treatments remain unknown. Using the methods of field trials and randomized blocks design, we quantified salt accumulation in the aboveground biomass, and the biochemical and nutritive value of field-cultivated S. salsa in arid northwestern China under irrigation with water of different salinities [i.e., freshwater or water containing10, 20, 30, or 40 g/L NaCl). The fresh and dry weights of S. salsa increased, then decreased, with increase in salinity. The salt content of the plant's aboveground biomass increased to a constant range and, thus, the salt extraction of S. salsa was relatively stable under different salinities of irrigation water. Under the experimental conditions, the crude protein content significantly increased to 9.45% dry weight (DW) and then decreased to 6.85% DW, with an increase in salinity (p < 0.05). The neutral detergent fiber (42.93%-50.00% DW) and acid detergent fiber (34.76%-39.70% DW) contents were suitable for forage. The contents of trace elements, such as copper and zinc, were significantly increased after irrigation with saline water (p < 0.05). The forage of S. salsa is of high nutritive value for livestock, and contains low concentrations of anti-nutrients. Therefore, S. salsa can be considered for cultivation in saline soils irrigated with saline water. In addition, it provides a viable additional source of fodder in arid regions, where the availability of freshwater and non-saline arable land is limited.

3D in vivo Magnetic Particle Imaging of Human Stem Cell-Derived Islet Organoid Transplantation Using a Machine Learning Algorithm.

Stem cell-derived islet organoids constitute a promising treatment of type 1 diabetes. A major hurdle in the field is the lack of appropriate in vivo method to determine graft outcome. Here, we investigate the feasibility of in vivo tracking of transplanted stem cell-derived islet organoids using magnetic particle imaging (MPI) in a mouse model. Human induced pluripotent stem cells-L1 were differentiated to islet organoids and labeled with superparamagnetic iron oxide nanoparticles. The phantoms comprising of different numbers of labeled islet organoids were imaged using an MPI system. Labeled islet organoids were transplanted into NOD/scid mice under the left kidney capsule and were then scanned using 3D MPI at 1, 7, and 28 days post transplantation. Quantitative assessment of the islet organoids was performed using the K-means++ algorithm analysis of 3D MPI. The left kidney was collected and processed for immunofluorescence staining of C-peptide and dextran. Islet organoids expressed islet cell markers including insulin and glucagon. Image analysis of labeled islet organoids phantoms revealed a direct linear correlation between the iron content and the number of islet organoids. The K-means++ algorithm showed that during the course of the study the signal from labeled islet organoids under the left kidney capsule decreased. Immunofluorescence staining of the kidney sections showed the presence of islet organoid grafts as confirmed by double staining for dextran and C-peptide. This study demonstrates that MPI with machine learning algorithm analysis can monitor islet organoids grafts labeled with super-paramagnetic iron oxide nanoparticles and provide quantitative information of their presence in vivo.

Circ_WWC3 overexpression decelerates the progression of osteosarcoma by regulating miR-421/PDE7B axis.

BACKGROUND: Emerging evidence has shown that circular RNAs (circRNAs) are vital regulators in osteosarcoma (OS) progression. However, the effects of circ_WWC3 in OS have not been explored. In this research, the functions and mechanisms of circ_WWC3 in OS were investigated. METHODS: Quantitative reverse trancription polymerase chain reaction (qRT-PCR) was adopted to determine the levels of circ_WWC3, WW and WWC3 mRNA, miR-421, and phosphodiesterase 7B (PDE7B) mRNA. RNase R assay was used to determine the characteristic of circ_WWC3. Colony formation assay and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay were applied for cell growth. Transwell assay was performed for cell migration and invasion. Flow cytometry analysis was utilized for cell apoptosis. Western blot assay was conducted for the levels of apoptosis-related proteins and PDE7B protein. Dual-luciferase reporter assay was carried out to analyze the targeting relationship between miR-421 and circ_WWC3 or PDE7B. The murine xenograft model was established to explore the effect of circ_WWC3 in vivo. RESULTS: Compared to normal tissues and cells, circ_WWC3 and PDE7B were downregulated in OS tissues and cells. Overexpression of circ_WWC3 or PDE7B suppressed OS cell growth, migration, and invasion and promoted apoptosis in vitro. Regarding the mechanism analysis, circ_WWC3 positively modulated PDE7B expression by targeting miR-421. MiR-421 overexpression restored the impacts of circ_WWC3 on OS cell growth, metastasis, and apoptosis. Inhibition of miR-421 repressed the malignant behaviors of OS cells by targeting PDE7B. In addition, circ_WWC3 inhibited the tumorigenicity of OS in vivo. CONCLUSION: Circ_WWC3 overexpression slowed the development of OS by elevating PDE7B via sponging miR-421.

Artificial Intelligence Analysis of Magnetic Particle Imaging for Islet Transplantation in a Mouse Model.

PURPOSE: Current approaches to quantification of magnetic particle imaging (MPI) for cell-based therapy are thwarted by the lack of reliable, standardized methods of segmenting the signal from background in images. This calls for the development of artificial intelligence (AI) systems for MPI analysis. PROCEDURES: We utilize a canonical algorithm in the domain of unsupervised machine learning, known as K-means++, to segment the regions of interest (ROI) of images and perform iron quantification analysis using a standard curve model. We generated in vitro, in vivo, and ex vivo data using islets and mouse models and applied the AI algorithm to gain insight into segmentation and iron prediction on these MPI data. In vitro models included imaging the VivoTrax-labeled islets in varying numbers. In vivo mouse models were generated through transplantation of increasing numbers of the labeled islets under the kidney capsule of mice. Ex vivo data were obtained from the MPI images of excised kidney grafts. RESULTS: The K-means++ algorithms segmented the ROI of in vitro phantoms with minimal noise. A linear correlation between the islet numbers and the increasing prediction of total iron value (TIV) in the islets was observed. Segmentation results of the ROI of the in vivo MPI scans showed that with increasing number of transplanted islets, the signal intensity increased with linear trend. Upon segmenting the ROI of ex vivo data, a linear trend was observed in which increasing intensity of the ROI yielded increasing TIV of the islets. Through statistical evaluation of the algorithm performance via intraclass correlation coefficient validation, we observed excellent performance of K-means++-based model on segmentation and quantification analysis of MPI data. CONCLUSIONS: We have demonstrated the ability of the K-means++-based model to provide a standardized method of segmentation and quantification of MPI scans in an islet transplantation mouse model.

Putting the Blame on Others in Suicide Notes From Shanghai and Wuhan, China.

Background: Suicide note leavers sometimes blame others for their death. The blame could reveal extrinsic suicide risk factors and thus countermeasures may be ascertained. Aims: This study included suicide note leavers in Shanghai and Wuhan (n = 555) to examine (a) who was inclined to put the blame (b) on whom and (c) for what reason(s). Method: Logistic regressions were used to compare the note leavers who blamed others with those who did not. Cramer's V tests were used to examine the correlations between the note leavers' demographics and the targets of the blame. Results: Note leavers who used poisoning and cutting were more likely to blame others compared with those who used jumping, drowning, and hanging. Non-native note leavers tended to more frequently blame social problems and their workplaces compared with the natives. The common reasons for the blame on nonfamily members, children, and lovers/spouses were being mistakenly blamed for something, being disobedient, and having conflicts/hatred, respectively. Limitations: Some blame could have been made under the influence of psychiatric disorder/substances, and thus potentially deviated from the facts. Conclusion: Emotional/marriage consultations and family-therapy services should be made available to females experiencing love/family crises. Mental health services in the workplace could help reduce suicide risks.

Mortalities of methamphetamine, opioid, and ketamine abusers in Shanghai and Wuhan, China.

Studies on the mortalities of drug abusers in China are scarce. This study explores the deaths of methamphetamine, opioid, and ketamine abusers in Shanghai (2004-2017) and Wuhan (2005-2017). Chi-square/Fisher's exact tests were used to compare the differences in terms of region, gender, age, cause of death, and the method used in the last drug abuse. Poisson regression models were used to estimate the rate ratios ("RRs") and annual percentage changes ("APCs"). 314 heroin, 43 methamphetamine, and 4 ketamine abusers were included. Furthermore, simultaneously, 6 abusers used heroin and methamphetamine, and 7 abusers used methamphetamine and ketamine. Heroin-related deaths have declined in Shanghai (APC, -16.1; 95 % CI, -18.4 to -11.3) and Wuhan (APC, -16.0; 95 % CI, -18.9 to -10.6), whereas methamphetamine-related deaths have increased in Wuhan (APC, 12.8; 95 % CI, 0.0 to 29.2). On the whole, in the two cities, males were more frequently observed than females in heroin-related deaths (4.4, 230/52). However, the gender ratios for methamphetamine- (1.8, 34/19) and ketamine-related deaths (1.2, 6/5) were close to one. In view of the mortality rates of the drug abusers in most Chinese cities were still unclear, it is thus important to improve mortality surveillance of the drug abusers at the national level.

The primary motivation of sexual homicide offenders in China: Was it for sex, power and control, anger, or money?

BACKGROUND: Information on sexual homicides in China is scarce, particularly with respect to the offenders' primary motivation for the offence. AIMS: Our aim was to examine how offence characteristics of sexual murderers relate to different primary motivations. METHODS: The offender, victim, and offence characteristics of 67 sexual homicides, derived from police data of three regions and published case reports (1994-2017), were explored and compared according to offenders' primary motivation. RESULTS: Heterosexual sexual homicides were committed by offenders who were mainly driven by power and control, sex, and anger, compared with slightly over half of those who motivated primarily by financial gain. Sexual murderers whose killing was mainly driven by sex, money, and anger were more likely to target strangers, whereas offenders who motivated primarily by power and control were more likely to target an intimate partner. Victim abduction was more frequently seen in offenders who were motivated predominantly by money than other motives. Relative to financial gain as primary motivation, sexual killers who were mainly driven by sex, power and control, and anger were more likely to mutilate their victim. IMPLICATIONS FOR PRACTICE: This study adds data to a field rarely studied anywhere and hardly at all in China. Findings may offer some help for criminal investigators who need to prioritise their investigative strategies. Once the individual is convicted and sentenced, they may help direct management and/or therapeutic strategies.

Sexual Homicides in China: Exploring the Offender, Victim, and Offense Characteristics.

Nearly all information available on sexual homicides are limited to studies conducted in the West. Little is known about sexual homicides that occurred in China. The current study is arguably the first to explore the offender, victim, and offense characteristics of Chinese sexual homicides. Over a period of 23 years (1994 to 2016), the data of 59 cases collected from two data sources (i.e., published Chinese case reports and police data) are examined. Findings indicate that heterosexual assaults are far more prevalent in Chinese sexual homicides. Sexual murderers who averagely aged 32.44 years are mostly males (97%), single (67%), secondary school educated (68%), and with no prior overall (80%) and sexual (88%) convictions. The victims are mostly females (83%) with mean age of 35.35 years. The frequently observed offending patterns include strangers as victim choice (63%), con tactics as victim approach method (57%), sexual pleasure as primary motivation (49%), and personal weapons as murder weapon of choice (41%). Vaginal penetration is a commonly observed sexual assault (88%) and victim body mutilation is also not uncommon (47%). Three case examples are also presented to illustrate the diverse nature and offending patterns of Chinese sexual homicide offenders.

Application of the injury scales in homicides.

Injury scales have two main applications on homicide investigations, namely, to evaluate the severity of the victims' injuries and to identify the profiles of the offenders. However, few studies have examined the quality of the various injury scales in serving the two purposes. In this study, homicides from Shanghai and Wuhan, China (n=439) have been used to examine nine injury scales. The results showed that seven out of the nine scales were useful. Compared to one-to-one homicides, offenders who killed two or more people tended to inflict more fatal injuries and made fewer number of attacks on the victims' heads and necks. Among all homicide cases, victims of stranger homicides tended to have fewer total number of wounds, as well as less severity of wounds on the heads, necks, and faces compared to those of intimate partner homicides. As to one-to-one homicides, only the severity of wounds on the face could assist to distinguish stranger homicides from intimate partner homicides. When a male victim died in a one-to-one homicide, the high number of total wounds along with the high number and severity of wounds on the head and neck could indicate that the offender was a female.

SOX10 induced Nestin expression regulates cancer stem cell properties of TNBC cells.

The mechanisms modulating the cancer stem cell (CSC) properties of triple negative breast cancer (TNBC) cells were not fully understood. In this study, we performed data mining in Breast Cancer Gene-Expression Miner v4.0 and found that TNBC tumors had significantly higher NES mRNA expression than other breast cancer subtypes. Pooled data suggested that NES mRNA expression is associated worse metastatic relapse (MR) free survival and also worse any event (AE) free survival in TNBC patients. Following data mining in multiple big data databases confirmed a positive correlation between SOX10 mRNA expression and NES mRNA expression in breast cancer tissues. In addition, the expression of SOX10 mRNA is significantly higher in TNBC tissues than in other breast cancer subtypes. SOX10 overexpression resulted in Nestin upregulation at both mRNA and protein levels. Bioinformatic analysis predicted a SOX10 binding site in NES promoter and the following dual luciferase assay verified the binding site. Functionally, SOX10 overexpression substantially increased CSC properties of TNBC cells, while SOX10 knockdown decreased the CSC properties, in terms of CD24-/CD44+ cell ratio and tumorsphere-forming capabilities. Enforced Nestin expression partly counteracted the effect of SOX10 knockdown on reducing the CSC properties. Based on these findings, we infer that SOX10 regulates cancer stem cell properties of TNBC cells via inducing Nestin expression.

Association between pulmonary fibrosis and osteoporosis in the elderly people: A case-control study.

In this case-control study, we evaluated the association between osteoporosis and pulmonary fibrosis in the elderly. Participants were divided into a test group and a control group depending on bone mineral density and grid pattern changes of pulmonary fibrosis. We measured general conditions, related risk factors, serum biochemical index, grid pattern changes of double lungs, pulmonary function, arterial oxygen pressure (PO2), and bone mineral density of participates, and investigated the data through statistical analysis on SPSS 17.0 (SPSS Inc, Chicago, Illinois, USA). Significant differences were observed between groups in all collected indices except PO4. The ratio of pulmonary function disorder was higher in the test group versus the control group (12.0% vs 4.3%). Logistic regression shows that pulmonary fibrosis is a risk factor of osteoporosis, independent of age, sex, body mass index, smoking status, diabetes mellitus, alkaline phosphatase, glycosylated hemoglobin, Ca, PO4, tumor necrosis factor-α, vitamin D total, ventilation disorder, diffusive dysfunction, and hypoxemia. Senile osteoporosis is closely associated with pulmonary fibrosis, diabetes mellitus, smoking, sexuality, age, and body mass index. Pulmonary fibrosis modestly affects the incidence of osteoporosis and thus is a more promising predictor of osteoporosis.

Downregulation of AATK mediates microRNA-558-induced resistance of A549 cells to radiotherapy.

The deregulation of microRNAs (miRNAs) is often implicated in the control of sensitivity to radiotherapy. The objective of the present study was to identify the association between miR­558 and apoptosis­associated tyrosine kinase (AATK), and their importance in regulating the development of resistance to radiotherapy. The current study demonstrated that AATK, a radiosensitization-associated gene, is a target of miR­558 in lung cancer cells, using in silico analysis and a luciferase reporter system. Furthermore, it was determined that transfection of 30 or 50 nM miR­558 mimics and AATK specific siRNA markedly suppressed the mRNA and protein expression of AATK. To determine whether miR­558 was required for lung cancer cell radioresistance, A549 cells were treated with different doses of ionizing radiation, from 0 to 10 Gy, following transfection with miR­558 mimics or AATK specific siRNA. It was determined that the administration of miR­558 mimics or AATK specific siRNA alone did not significantly alter the survival rate of the cells. By contrast, in the cells exposed to 4, 6 or 8 Gy, the administration of miR­558 mimics or AATK specific siRNA significantly promoted cell survival rate and overexpression of AATK reversed this effect. In conclusion, these data demonstrate that the miR­558/AATK cascade is important for the radiosensitization of lung cancer cells and may be a potential radiotherapy target.

Association of p16 gene methylation with prostate cancer risk: a meta-analysis.

PURPOSE: The tumor suppressor gene p16 is frequently silenced and inactivated by hypermethylation in human cancers, including prostate cancer. However, the association between the methylation status of p16 and prostate cancer risk remains ambiguous. This study aimed to assess the association of p16 methylation with prostate cancer risk by a comprehensive metaanalysis. METHODS: Relevant studies were identified by searching PubMed, Embase and Web of Science databases before October 2014 with no restrictions. The strength of the association between p16 methylation and prostate cancer risk was assessed by combined odds ratio (OR) and 95 % confidence interval (CI). The between-study heterogeneity and the contributions of single studies to the final results were tested by chi-square-based Q test and sensitivity analyses, respectively. Publication bias was evaluated by funnel plots. RESULTS: A total of 1,296 samples from 12 independent studies were enrolled in the present metaanalysis. Overall, a significant association was observed between p16 methylation and prostate cancer risk (OR=3.06; 95% CI:1.34- 6.98;p=0.008). Stratified analyses by ethnic groups further revealed that prostate cancer risk was increased for individuals carrying the methylated p16 compared with those with unmethylated p16 in Caucasian populations (OR=2.51;95% CI:1.01-6.26;p=0.047) and Asian populations (OR=9.50;95% CI:1.78-50.61;p=0.008). CONCLUSIONS: This study identified a strong association of p16 methylation with prostate cancer risk and suggested that p16 methylation might be a potential biomarker for prostate cancer.

Carbon monoxide poisoning as a cause of death in Wuhan, China: A retrospective six-year epidemiological study (2009-2014).

Carbon monoxide (CO) poisoning is a common cause of death globally, and case reports and empirical studies on CO poisoning are widely examined. However, to the authors' knowledge, CO poisoning deaths in the mainland China are scarcely studied. Therefore, this study aims to explore the incidence trend of CO poisoning deaths that occurred in Wuhan - a mega city in Central China - for a six-year period (2009-2014). This arguably is the first comprehensive study to provide an overall analysis of CO poisoning deaths that sampled the mainland Chinese population. Using the data provided by the legal physicians who are employed in nine districts of Wuhan, a total of 131 cases of CO poisoning that resulted in the death of 156 victims are collected. Out of the total, 76 cases (97 deaths) are classified as accidents, 49 cases (51 deaths) are suicides, three cases (four deaths) are homicides, one case (three deaths) is homicide-suicide, and one case (one death) is classified as undetermined. Male victims are found to be the dominant sex group (53.5%; N=83); with a mean age of 44.9 years, while female victims averagely aged 46.1 years. The highest death occurring month is in January, and followed by February and December. Coal or charcoal burning is found to be the major cause of suicide CO poisoning death (66.7%), while fire accident is the major cause of accidental CO poisoning death (60.8%) in Wuhan during the six-year period.

Inflammatory cytokine expression in the quadriceps of rats with posttraumatic knee stiffness: A preliminary study.

The primary purpose of this study was to investigate cytokine expression in the quadriceps of rats with posttraumatic knee stiffness (PTKS) and to determine the effect of exercise training on these cytokines at different follow-up time points. The PTKS rats were randomly assigned into two even groups. The treatment group received exercise training, while the control group received no treatment. Quadriceps specimens were harvested randomly from each group at 8, 12, 16, and 20 weeks. RT-qPCR and immunohistochemical analyses were used to assess the protein and mRNA expression levels of the cytokines IL-1, IL-2, TNF-α, COX-1, and COX-2. TNF-α immunostaining did not differ between the treated and control group tissues, whereas weak immunostaining was observed for all other cytokines in the specimens from the treatment group compared with those from the control group at approximately 12 and 20 weeks. The cytokine levels decreased at approximately 8 weeks in the treatment group, whereas these levels remained elevated or plateaued in the control group. These differences were statistically significant (p<0.05). This study demonstrated that the expression of cytokines IL-1, IL-2, COX-1, and COX-2 increased in the quadriceps of rats with PTKS and that exercise training affected the observed profile trends of these cytokines.