Spatial patterns of intrinsic brain activity in rats with capsular stroke.

BACKGROUND: To explore the neural changes of brain activity in rats with circumscribed capsular infarcts to find a new therapeutic target for promoting the functional recovery. METHODS: A total of 18 capsular infarct rats and 18 normal rats were conducted in this study. All animal use procedures were strictly in accordance with the guide for the care and use of laboratory animals. After establishing the photothrombotic capsular infarct model, the functional magnetic resonance imaging (fMRI) data were collected and analyzed. RESULTS: The fMRI results indicated that the passive movement would induce strong activation in caudate, putamen, frontal association somatosensory cortex, thalamus dorsolateral, and thalamus midline dorsal in control group, and the passive movement would only induce limited activation mostly in somatosensory cortex, thalamus dorsolateral, and thalamus midline dorsal in capsular infarct models. Capsular infarct makes the cortical activity weaken in sensory-related cortex and subcortical nuclei, including capsular area and thalamus. CONCLUSIONS: Such findings imply that the posterior limb of internal capsule (PLIC) is connected to these structures in function, interacts together with them, and, accordingly, the lesion of PLIC manifests the related symptoms.

Genome-wide association analysis for grain moisture content and dehydration rate on maize hybrids.

For mechanized maize production, a low grain water content (GWC) at harvest is necessary. However, as a complex quantitative trait, understand the genetic mechanism of GWC remains a large gap, especially in hybrids. In this study, a hybrid population through two environments including 442 F1 was used for genome-wide association analysis of GWC and the grain dehydration rate (GDR), using the area under the dry down curve (AUDDC) as the index. Then, we identified 19 and 17 associated SNPs for GWC and AUDDC, including 10 co-localized SNPs, along with 64 and 77 pairs of epistatic SNPs for GWC and AUDDC, respectively. These loci could explain 11.39-68.2% of the total phenotypic variation for GWC and 41.07-67.02% for AUDDC at different stages, whose major effect was the additive and epistatic effect. By exploring the candidate genes around the significant sites, a total of 398 and 457 possible protein-coding genes were screened, including autophagy pathway and auxin regulation-related genes, and five inbred lines with the potential to reduce GWC in the combined F1 hybrid were identified. Our research not only provides a certain reference for the genetic mechanism analysis of GWC in hybrids but also provides an added reference for breeding low-GWC materials. Supplementary Information: The online version contains supplementary material available at 10.1007/s11032-022-01349-x.

Preparation and Characterization of Nano-Fe3O4 and Its Application for C18-Functionalized Magnetic Nanomaterials Used as Chromatographic Packing Materials.

A new type of magnetic nanomaterial with Fe3O4 as the core and organic polymer as the shell was synthesized by seed emulsion polymerization. This material not only overcomes the problem of insufficient mechanical strength of the organic polymer, it also solves the problem that Fe3O4 is prone to oxidation and agglomeration. In order to make the particle size of Fe3O4 meet the requirement of the seed, the solvothermal method was used to prepare Fe3O4. The effects of the reaction time, amount of solvent, pH value, and polyethylene glycol (PEG) on the particle size of Fe3O4 were investigated. In addition, in order to accelerate the reaction rate, the feasibility of preparing Fe3O4 by microwave was studied. The results showed that under the optimum conditions, the particle size of Fe3O4 could reach 400 nm and had good magnetic properties. After three stages of oleic acid coating, seed emulsion polymerization, and C18 modification, the obtained C18-functionalized magnetic nanomaterials were used for the preparation of the chromatographic column. Under optimal conditions, stepwise elution significantly shortened the elution time of sulfamethyldiazine, sulfamethazine, sulfamethoxypyridazine, and sulfamethoxazole while still achieving a baseline separation.

Low-dose nifedipine rescues impaired endothelial progenitor cell-mediated angiogenesis in diabetic mice.

It is of great clinical significance to develop potential novel strategies to prevent diabetic cardiovascular complications. Endothelial progenitor cell (EPC) dysfunction is a key contributor to diabetic vascular complications. In the present study we evaluated whether low-dose nifedipine could rescue impaired EPC-mediated angiogenesis and prevent cardiovascular complications in diabetic mice. Diabetes was induced in mice by five consecutive injections of streptozotocin (STZ, 60 mg·kg-1·d-1, i.p.). Diabetic mice were treated with low-dose nifedipine (1.5 mg·kg-1·d-1, i.g.) for six weeks. Then, circulating EPCs in the peripheral blood were quantified, and bone marrow-derived EPCs (BM-EPCs) were prepared. We showed that administration of low-dose nifedipine significantly increased circulating EPCs, improved BM-EPCs function, promoted angiogenesis, and reduced the cerebral ischemic injury in diabetic mice. Furthermore, we found that low-dose nifedipine significantly increased endothelial nitric oxide synthase (eNOS) expression and intracellular NO levels, and decreased the levels of intracellular O2.- and thrombospondin-1/2 (TSP-1/2, a potent angiogenesis inhibitor) in BM-EPCs of diabetic mice. In cultured BM-EPCs, co-treatment with nifedipine (0.1, 1 µM) dose-dependently protected against high-glucose-induced impairment of migration, and suppressed high-glucose-induced TSP-1 secretion and superoxide overproduction. In mice with middle cerebral artery occlusion, intravenous injection of diabetic BM-EPCs treated with nifedipine displayed a greater ability to promote local angiogenesis and reduce cerebral ischemic injury compared to injection of diabetic BM-EPCs treated with vehicle, and the donor-derived BM-EPCs homed to the recipient ischemic brain. In conclusion, low-dose nifedipine can enhance EPCs' angiogenic potential and protect against cerebral ischemic injury in diabetic mice. It is implied that chronic treatment with low-dose nifedipine may be a safe and economic manner to prevent ischemic diseases (including stroke) in diabetes.

Investigation and molecular identification of Anisakis infection in marine fish sold in Fuxin, Liaoning Province / 中国热带医学

@#Abstract: Objective　To investigate the infection of Anisakis in marine fish sold in Fuxin, and conduct molecular identification and evolutionary tracing of third-stage larvae to determine Anisakis species. Methods　From 2018 to 2021, marine fish sold in the market were collected randomly, and the third stage larvae of Anisakis were detected in marine fish sold in the market by direct dissection, and the morphological characteristics were used to preliminarily identify species by microscopy; the total DNA was extracted, the internal transcribed spacer sequence of the ribosomal DNA of Anisakis was amplified, and the sequence alignment and evolution analysis were carried out. Results　A total of 289 market-sold sea fish samples of marine fish sold in the market were dissected and 84 samples of Anisakis were detected with a detection rate of 29.1%, of which the infection rates of hairtail and small yellow croaker were higher, at 41.4% and 41.2%, respectively. BLAST comparison of 28 sequences revealed eight species of anisakids, including Anisakis pegreffii, Anisakis simplex, Anisakis typical, Raphidascaris trichiurid, Contracaecum muraenesoxi, Hysterothylcaium zhoushanensis, Hysterothylacium amoyense and Hysterothylcaium fabri,belonging to the genera Anisakis and Hysterothylacium. The phylogenetic tree constructed from 28 sequences generally formed two topological branches, with Anisakis pegreffii, Anisakis simplex, and Anisakis typical forming three separate clusters as the topology branch of Anisakis genus. However, meanwhile, Hysterothylacium, Contracaecum, and Raphidascaris formed a separate topological branch. Conclusions　The marine fish sold in Fuxin City have severe anisakid infection, with a wide variety of anisakid species, the dominant species being Anisakis pegreffii.

Nanomaterials with Excellent Adsorption Characteristics for Sample Pretreatment: A Review.

Sample pretreatment in analytical chemistry is critical, and the selection of materials for sample pretreatment is a key factor for high enrichment ability, good practicality, and satisfactory recoveries. In this review, the recent progress of the sample pretreatment methods based on various nanomaterials (i.e., carbon nanomaterials, porous nanomaterials, and magnetic nanomaterials) with excellent adsorption efficiency, selectivity, and reproducibility, as well as their applications, are presented. Due to the unique nanoscale physical-chemical properties, magnetic nanomaterials have been used for the extraction of target analytes by easy-to-handle magnetic separation under a magnetic field, which can avoid cumbersome centrifugation and filtration steps. This review also highlights the preparation process and reaction mechanism of nanomaterials used in the sample pretreatment methods, which have been applied for the extraction organophosphorus pesticides, fluoroquinolone antibiotics, phenoxy carboxylic acids, tetracycline antibiotics, hazardous metal ions, and rosmarinic acid. In addition, the remaining challenges and future directions for nanomaterials used as sorbents in the sample pretreatment are discussed.

[Influence of Different Soil Conditioner on the Transfer and Transformation of Cadmium and Phthalate Esters in Soil].

This study examines the existing common form of soil pollution, combined organic and inorganic pollution. Cadmium (Cd) is the most important inorganic element in soil pollution. Due to the widespread use of plastic film, phthalates have become the main organic pollutants in soil. Pot experiments were conducted with purple soil from southwest China, and Chinese cabbage was used as a biological indicator. Different concentration gradients of Cd and diethylhexyl phthalate (DEHP) was used as foreign pollutants. The soil was treated with one of the six common soil conditioners, namely potassium feldspar powder, oyster shell powder, biological carbon powder (biochar), calcium, potassium carbonate, and calcium phosphate, to examine the effect of conditioners on cadmium morphology, DEHP content in contaminated soil, and cadmium and DEHP absorption in Chinese cabbage. The results showed that biochar is the optimal soil conditioner for the remediation of cadmium-phthalate composite pollution in purple soil. Subsequently, the effects of soil biochar content on cadmium pollution and phthalate ester migration were studied. Uncontaminated control soil, Cd-contaminated soil, and DEHP-contaminated soil were examined by pot experiments, and biochar treatments with mass fraction of 0%, 0.5%, 1%, 3%, and 5% added to cadmium contaminated soil were used to determine its influence on Cd morphology and DEHP content of contaminated soil.

Outcome prediction using the Mortality in Emergency Department Sepsis score combined with procalcitonin for influenza patients / Predicción del resultado utilizando la puntuación de mortalidad por sepsis en la unidad de urgencias combinada con procalcitonina en los pacientes con gripe

Background: Severe influenza is often associated with bacterial coinfection and can trigger sepsis, which increases the severity, complexity and mortality of the disease. To determine an effective method for predicting 28-day mortality of emergency department (ED) patients with influenza, we investigated the Mortality in Emergency Department Sepsis (MEDS) score, procalcitonin (PCT) and other relevant biomarkers. Methods: We conducted a retrospective, observational, monocentric study, and the endpoint was 28-day mortality. Independent predictors were identified and a new combination predictive model was created both by logistic regression, and the model was evaluated by a receiver operating characteristic (ROC) curve. Results: A total of 364 consecutive ED admitted patients with influenza were enrolled and 45 patients died within 28 days. For predicting 28-day mortality, the MEDS score and PCT were independent predictors with adjusted odds ratio of 1.318 (95% CI 1.206-1.439) and 1.038 (95% CI 1.010-1.065), and with AUCs of 0.817 (95% CI 0.756-0.878) and 0.793 (95% CI 0.725-0.861), respectively. The new combination of the MEDS score with PCT significantly improved the efficacy for predicting 28-day mortality with an AUC of 0.857 (95% CI 0.809-0.905), and was superior to the SOFA score with an AUC of 0.837 (95% CI 0.779-0.894). Conclusion: The MEDS score and PCT, especially when combined, perform well for predicting mortality of ED admitted patients with influenza

Antecedentes: La gripe severa se asocia a menudo a la coinfección bacteriana, pudiendo desencadenar sepsis, lo cual incrementa la gravedad, la complejidad y la mortalidad de la enfermedad. Para determinar un método efectivo de predecir la mortalidad a 28 días de los pacientes con gripe en la unidad de urgencias (ED), investigamos la puntuación de mortalidad por sepsis en la unidad de urgencias (MEDS), procalcitonina (PCT) y otros biomarcadores relevantes. Métodos: Realizamos un estudio retrospectivo, observacional y unicéntrico, cuya evaluación clínica fue la mortalidad a 28 días. Identificamos factores predictivos independientes, creamos un nuevo modelo predictivo combinado por regresión logística, y evaluamos el modelo mediante una curva ROC. Resultados: Incluimos a un total de 364 pacientes consecutivos ingresados en la ED, de los cuales 45 fallecieron en el plazo de 28 días. Para predecir la mortalidad a 28 días, la puntuación MEDS y PCT fueron factores predictivos independientes con odds ratio ajustados de 1,318 (IC 95%: 1,206-1,439) y 1,038 (IC 95%: 1,010-1;065), y ABC de 0,817 (IC 95%: 0,756-0,878) y 0,793 (IC 95%: 0,725-0,861), respectivamente. La nueva combinación de la puntuación MEDS y PCT mejoró significativamente la eficacia para predecir la mortalidad a 28 días con ABC de 0,857 (IC 95%: 0,809-0,905), siendo superior a la puntuación SOFA con ABC de 0,837 (IC 95%: 0,779-0,894). Conclusión: La puntuación MEDS y PCT, especialmente cuanto se combinan, constituyen una buena predicción de la mortalidad de los pacientes ingresados en la ED con gripe

[Spatial-temporal evolution characteristics and influencing factors of county-scale environmental pollution in Jilin Province, Northeast China]. / å‰æž—çœåŽ¿åŸŸçŽ¯å¢ƒæ±¡æŸ“çš„æ—¶ç©ºæ¼”å˜ç‰¹å¾ä¸Žå½±å“å› ç´ .

Based on pollutant emissions and social-economic data during 2011 and 2015, we selec-ted the total emission index of four pollutants, including the industrial source, agricultural source, and urban living source. The comprehensive index of environmental pollution and sub-source pollution index were built to reflect the pollution of Jilin Province at county scale. Furthermore, the Moran's I, spatial-temporal transition analysis, and standard deviation ellipse analysis were used to quantitatively examine the spatial-temporal evolutionary characteristics of environmental pollution in Jilin Province. A spatial econometric model was built to analyze the influencing factors of environmental pollution. The results showed that environmental pollution in Jilin Province presented spatial agglomeration characteristics, which was mainly concentrating in central areas such as Changchun and Jilin cities. There were obvious regional differences in the spatial distribution of environmental pollution from different sources. There were spatial correlation of the total environmental pollution and agricultural source pollution separately at county scale in Jilin Province. The correlation structure stability of the spatial distribution of environmental pollution was high, which had path locking characteristic. The spatial distribution of environmental pollution showed a northwest-southeast distribution pattern and a spread from the center to the surroundings. The barycenter of environmental pollution moved in the area between 43.65-43.66° N and 125.83-125.84° E. The level of regional economic development, urbanization level and agricultural production were main driving factors for county-scale environmental pollution and industrial source pollution. The adjustment and optimization of industrial structure had improved the overall environmental pollution and industrial source pollution situation. Overuse of chemical fertilizers significantly increased agricultural source pollution. The level of economic development and urbanization were the main drivers of urban living source pollution. The overall environmental pollution and agricultural source pollution had spatial diffusion effects, and their pollution status was closely related to the overall environmental pollution and agricultural source pollution status in adjacent areas, respectively.

Outcome prediction using the Mortality in Emergency Department Sepsis score combined with procalcitonin for influenza patients.

BACKGROUND: Severe influenza is often associated with bacterial coinfection and can trigger sepsis, which increases the severity, complexity and mortality of the disease. To determine an effective method for predicting 28-day mortality of emergency department (ED) patients with influenza, we investigated the Mortality in Emergency Department Sepsis (MEDS) score, procalcitonin (PCT) and other relevant biomarkers. METHODS: We conducted a retrospective, observational, monocentric study, and the endpoint was 28-day mortality. Independent predictors were identified and a new combination predictive model was created both by logistic regression, and the model was evaluated by a receiver operating characteristic (ROC) curve. RESULTS: A total of 364 consecutive ED admitted patients with influenza were enrolled and 45 patients died within 28 days. For predicting 28-day mortality, the MEDS score and PCT were independent predictors with adjusted odds ratio of 1.318 (95% CI 1.206-1.439) and 1.038 (95% CI 1.010-1.065), and with AUCs of 0.817 (95% CI 0.756-0.878) and 0.793 (95% CI 0.725-0.861), respectively. The new combination of the MEDS score with PCT significantly improved the efficacy for predicting 28-day mortality with an AUC of 0.857 (95% CI 0.809-0.905), and was superior to the SOFA score with an AUC of 0.837 (95% CI 0.779-0.894). CONCLUSION: The MEDS score and PCT, especially when combined, perform well for predicting mortality of ED admitted patients with influenza.

Recipient-Derived Allo-iTregs Induced by Donor DCs Effectively Inhibit the Proliferation of Donor T Cells and Reduce GVHD.

To compare the potency of recipient-derived, antigen-specific regulatory T cells induced by different dendritic cells (DCs; iTregs) and freshly isolated natural regulatory T cells (nTregs) in preventing mouse graft-versus-host disease (GVHD) after allogeneic bone marrow transplantation (BMT). CD4+ T cells from recipient BALB/c mice were stimulated with DCs from recipient BALB/c (syn-DCs), donor B6 (allo-DCs), and third-party C3H (third-party-DCs) mice to induce different iTregs. In parallel, nTregs were isolated from spleen cells of recipient BALB/c (syn-nTregs) and donor B6 (allo-nTregs) mice using magnetic-activated cell sorting. Mixed lymphocyte reaction (MLR) assays were performed to evaluate the suppressive ability of these various regulatory T cells (Tregs). Both the iTregs and nTregs were transfused to GVHD mice on Days 0, 1, 3, and 5. Body weight, GVHD score, and survival time were monitored. Peripheral Tregs were subsequently examined on Days 7, 14, 21, and 28 after BMT, while chimerism was evaluated on Days 14 and 60. Histopathology of colon, liver, and spleen were also performed. DCs markedly induced CD25+ and Foxp3+ expression on CD4+ T cells. The allo-DC-induced Tregs (allo-iTregs) suppressed the proliferation of alloreactive T cells better than the other iTregs/nTregs in MLR assays (P < 0.05). Meanwhile, transfusion of the allo-iTregs reduced the severity of GVHD (P < 0.05), increased survival time compared with the GVHD group (P < 0.05), and enhanced the chimerism proportion. On Day 28 after BMT, the allo-iTregs group had the highest frequency of peripheral Tregs (P < 0.05). Recipient-derived allo-iTregs induced by donor DCs included predominant clones that specifically recognized donor antigens. These allo-iTregs not only prevented GVHD by suppressing the proliferation of donor-alloreactive T cells, but also promoted engraftment, and prolonged the survival of GVHD mice. Anat Rec, 2018. © 2018 Wiley Periodicals, Inc. Anat Rec, 302:825-836, 2019. © 2018 Wiley Periodicals, Inc.

Organochlorine pesticides in ambient air from the littoral cities of northern China: Spatial distribution, seasonal variation, source apportionment and cancer risk assessment.

Concentrations, composition and seasonal variations of organochlorine pesticides (OCPs) in the atmosphere (particulate phase and gaseous phase) at coastal cities in northern China were determined. OCP transport from emission source areas and lifetime excess cancer risks by inhalation exposure to specific OCPs were also investigated. The annual average concentration of total OCPs in gaseous phase ranged from 1.0ng/m3 to 6.3ng/m3, with the peak observed in summer at most sites. Particulate phase concentrations ranged from 29.9pg/m3 to 103.3pg/m3, with the maximum found in the local heating period at most locations. The detection rates of gaseous samples were considerably higher than those of particulate ones. The dominant components included endosulfan (I and II), (α- and γ-) chlordane, pentachlorobiphenyl (PeCB), hexachlorobenzene (HCB), heptachlor, (α-, ß- and γ-) hexachlorocyclohexane (HCH), dichloro-diphenyl-trichloroethane (DDT) and their metabolic products. The specific ratios indicated different applications of DDT, technical HCH and endosulfan at most sites. Large differences in compositional profiles occurred in January (typical heating period) and July (representative non-heating period), and diurnal changes in component concentrations may have been influenced by local emission pattern. The potential source contribution function (PSCF) manifested seasonal concentrations of airborne OCPs affected by the input of potential sources in different regions. The emission sources with higher contribution probabilities to the sites were primarily distributed in the surrounding areas. The lifetime excess cancer risks for the local residents by inhalation exposure to specific components were not high, though the potential threat of α-HCH and HCB should be concerned. CAPSULE: Gaseous OCPs reached peak values in summer and dominated relative to particulate (PM10) values; meanwhile, surrounding sources affected air OCP concentrations, and cancer risks of OCPs by inhalation exposure were not high.

Liver-targeted delivery of liposome-encapsulated curcumol using galactosylated-stearate.

Liver-targeted drug delivery improves the efficacy of anti-liver cancer agents and reduces systemic toxicity by limiting the bioavailability of these drugs to within tumors. Liver targeting reagents with galactose residues, which selectively combine to asialoglyco protein receptors, have previously been used to improve liposome-encapsulated drug accumulation within liver cells. They lead to a reduction in liver cancer cell growth and have been used to cure certain hepatic diseases. In the present study, curcumol, which is the primary active component of Chinese traditional medicine Rhizoma zedoariae, was encapsulated in galactosylated-liposomes to enhance its anti-liver cancer efficacy. Galactosylated-liposomes and normal liposomes were labeled with propidium iodide. Galactosylated-liposomes with increasing concentrations of galactosylated-stearate (Gal-s) had a notably increased level of uptake in HepG2 cells (hepatoblastoma) compared with SGC-7901 (gastric cancer) and A549 (non-small cell lung cancer) cells. When the percentage of Gal-s reached 20%, liposome uptake plateaued. In the in vitro anti-liver cancer experiment, the anti-liver cancer efficacy of galactosylated-curcumol-liposomes increased significantly more compared with normal curcumol liposomes and free curcumol as indicated by cell survival rate and lactate dehydrogenase release rate. Collectively, these results demonstrate that galactosylated-liposomes are able to enhance the in vitro liver-targeting effect and anti-liver cancer efficacy of curcumol.

Improved quality of life and joint functions in patients with knee rheumatoid arthritis who underwent five portal arthroscopic synovectomy.

OBJECTIVES: To evaluate the outcomes of patients with rheumatoid arthritis (RA) of the knee who underwent five portal arthroscopic synovectomy, with or without post-operative negative pressure drainage (NPD). MATERIAL AND METHODS: A prospective clinical trial was performed. Patients with class I, II, and III RA of the knee were enrolled. They underwent five portal arthroscopic synovectomy. Post-operatively, they received either NPD (group A) or non-NPD (group B). Health assessment questionnaire (HAQ), disease activity score 28 (DAS 28), and Lysholm knee joint score were evaluated before the operations, and at six weeks, three months, and one year after the operations. RESULTS: A total of 36 patients were enrolled into the study, with 63.9% (23) female patients and mean age of 47.2 years old. All of the patients had clinical symptoms (joint swelling, pain, and dysfunction) for at least six months with poor responses to the traditional pharmaceutical therapy. There were 12, 16, and eight patients in class I, II, and III RA groups, respectively (six IA, six IB, eight IIA, eight IIB, four IIIA, and four IIIB). One year after the operation, patients had statistically significant improvements on HAQ, DAS 28, and Lysholm knee joint scores. More improvements were observed in patients with class I diseases. There were no statistically significant differences between group A and B. CONCLUSION: Five portal arthroscopic synovectomy could increase the quality of life, decrease disease activities, and improve joint functions in patients with RA. More benefits were observed in patients with early disease developments. Patients in the NPD group did not show more improvements compared to the patients in the non-NPD group.

VEGF and SEMA4D have synergistic effects on the promotion of angiogenesis in epithelial ovarian cancer.

BACKGROUND: Anti-angiogenesis therapy that targets VEGF is one of the important treatment strategies in advanced ovarian cancer. However, depending on the pharmaceutical agent, treatment can have undesirable side effects. SEMA4D has recently gained interest for its role in promoting angiogenesis. Here, we try to further understand the mechanism by which SEMA4D promotes angiogenesis in ovarian cancer. METHODS: Correlation and western blot assaya were used to detect the relationship between VEGF and SEMA4D in clinical tissues and cells. Vasculogenic mimicry and transwell migration analyses were used to detect the roles of VEGF, SEMA4D and plexin-B1 on vasculogenic mimicry and migration. Vascular density and SEMA4D expression was determined using immunofluorescence staining in clinical tissues of EOC. Western blot was used to detect the expressions of CD31, MMP2 and VE-cadherin. We also analyzed the relationship between VEGF-SEMA4D and malignant tumor prognosis. RESULTS: We found that knockdown of VEGF could suppress SEMA4D expression and that the expressions of VEGF and SEMA4D have a positive correlation in EOC cancer tissues. Vasculogenic mimicry and transwell migration analyses showed that SEMA4D and VEGF have a synergistic effect on the promotion of angiogenesis in A2780 and HUVEC cells. Soluble SEMA4D (sSEMA4D) could promote VM and migration in A2780 and HUVEC cells via the SEMA4D/plexin-B1 pathway, but the effect was not noted in stably transfected shR-plexin-B1 cells. In clinical tissues of EOC, the vascular density and SEMA4D/plexin-B1 expression were higher. When VEGF, SEMA4D and plexin-B1 was knocked down, the expression of CD31, MMP2 and VE-cadherin, which are the markers and initiators of angiogenesis and the epithelial-mesenchymal transition (EMT) process were reduced. VEGF and SEMA4D had a positive correlation with the malignant degree of ovarian cancer, and SEMA4D can serve as an independent prognostic factor. CONCLUSIONS: VEGF and SEMA4D have synergistic effects on the promotion of angiogenesis in epithelial ovarian cancer. Targeting VEGF and the SEMA4D signaling pathway could be important for the therapy for EOC.

Loss of Calreticulin Uncovers a Critical Role for Calcium in Regulating Cellular Lipid Homeostasis.

A direct link between Ca2+ and lipid homeostasis has not been definitively demonstrated. In this study, we show that manipulation of ER Ca2+ causes the re-distribution of a portion of the intracellular unesterified cholesterol to a pool that is not available to the SCAP-SREBP complex. The SREBP processing pathway in ER Ca2+ depleted cells remained fully functional and responsive to changes in cellular cholesterol status but differed unexpectedly in basal activity. These findings establish the role of Ca2+ in determining the reference set-point for controlling cellular lipid homeostasis. We propose that ER Ca2+ status is an important determinant of the basal sensitivity of the sterol sensing mechanism inherent to the SREBP processing pathway.

HLA-DRB1 shared epitope allele polymorphisms and rheumatoid arthritis: a systemic review and meta-analysis.

PURPOSE: A meta-analysis was preformed to determine which HLA-DRB1 alleles are associated with increased risk of rheumatoid arthritis (RA) in Asian patients. METHODS: Medline, PubMed, Central, EMBASE, Cochrane, and Google Scholar databases were searched until November 3, 2015 using the following keywords: rheumatoid arthritis; RA, HLA-DRB1; severity; treatment; and, prognosis. Randomized-controlled-trials, prospective, retrospective, cohort and case-controlled studies that examined the HLA-DRB1 allelic association with RA in Asians were included. The frequencies of allelic types and the shared epitope (SE) were compared between patients with or without RA. RESULTS: A total of 331 articles were identified after duplicates removed, and 40 studies, with 5470 RA patients and 5837 control patients, were included in the analysis. Pooled odds ratios (ORs) revealed the frequencies of HLA-DRB1*04 and *10 were higher in the RA group, and the frequency of *14 was lower in the RA group as compared to controls (*04: OR = 3.35, 95% CI: 2.28-3.99, p < .001; *10: OR = 2.08, 95% CI: 1.55-2.78, p < .001; *14: OR = 0.70, 95% CI: 0.54-0.90, p = .006). No associations were noted for *01 and *09. Pooled ORs revealed associations of *0101 (OR = 1.58), *0401 (OR = 2.17), *0404 (OR = 1.91), *0405 (OR = 3.73), *0410 (OR = 2.24), *1001 (OR = 1.78) and SE positive (OR = 2.38) with RA. HLA-DRB1 *14 subtypes did not show associations with RA. CONCLUSIONS: HLA-DRB1 allelic variations are associated with RA in Asian patients.

[Concentrations and Component Profiles PAHs in Surface Soils and Wheat Grains from the Cornfields Close to the Steel Smelting Industry in Handan, Hebei Province].

In this study, paired surface soil and mature wheat grain samples were collected in the cornfields near the large Handan Steel Manufacturer; and the total concentrations and compositional profiles of the parent PAHs were measured, then the spatial distribution characteristics and correlation with total organic carbon fractions in soil were determined. Accordingly, a preliminary source identification was performed, and the association between PAHs in surface soil and wheat grain was briefly discussed. The median concentration of total PAHs in surface soils from the cornfields of Handan was 398.9 ng x g(-1) (ranged from 123.4 ng x g(-1) to 1626.4 ng x g(-1), where around 18% and 10% of all the studied soil samples were over the corresponding quality criteria for total PAHs and B [a] P in soils, respectively. The MMW and HMW species were the main components in the compositional profiles of surface soils. Based on the specific isomeric ratios of PAHs species, coal/biomass combustion and transportation fuel (tail gas) were the dominant mixed sources for the local PAHs emission. The fractions of surface soil TOC had significant positive correlations with the total PAHs and also with the individual components with different rings. In addition, the median concentration of total PAHs in wheat grains collected in the cornfields near the Handan Steel Manufacture was 27.0 ng x g(-1) (ranged from 19.0-34.0 ng x g(-1)). The levels in wheat grains were not high, and lower than the related hygienic standards of food proposed by EU and China. The LMW and MMW PAHs with 2 to 4 rings occupied a larger proportion, more than 84% of the total PAHs, which was largely different from the component profiles in surface soils. This situation suggested that the local sources of PAHs in wheat grains may originate not only from surface soil via root absorption and internal transportation, but also from ambient air through dry and wet deposition on the leaf surface (stoma).

[Recent Distribution and Sources of Polycyclic Aromatic Hydrocarbons in Surface Soils from Yangtze River Delta].

A total of 243 surface soil samples collected from 11 cities in the Yangtze River Delta region were analyzed for the concentrations, spatial distribution, component profiles and emission sources of 29 PAH species. The analytical results indicated the total concentrations of PAHs in Yangtze River Delta fell in the range from 21. 0 ng x g(-1) to 3 578.5 ng x g(-1) with an arithmetic mean and standard deviation of 310.6 ng x g(-1) and 459.1 ng x g(-1), respectively. Our data showed spatial distribution of PAHs concentrations varied greatly in the region. In addition, the contents of PAHs were positively correlated with the total organic carbon fractions in topsoil. The sites with the highest levels of PAHs in the 11 cities studied were located in Suzhou with 759.0 ng x g(-1) +/- 132.9 ng x g(-1) ollowed by the areas of Wuxi and Shanghai, with the total PAHs concentrations of 565. 3 ng x g(-1) +/- 705.5 ng x g(-1) and 349.4 ng g(-1) 220. 1 ng-g(-1) respectively. The profiles of different components pointed to a predominant role of the species with 2-4 rings, and especially for the low molecular weight components with 2-3 rings. A preliminary identification on emission sources of local PAHs was performed by the specific ratios of isomeric species and principal component analysis (PCA). The results designated industrial coal and biomass combustion as the main mixed emission sources of PAHs in surface soils from Yangtze River Delta, and tail gas from transport as another major source in some areas.

AtKC1 and CIPK23 Synergistically Modulate AKT1-Mediated Low-Potassium Stress Responses in Arabidopsis.

In Arabidopsis (Arabidopsis thaliana), the Shaker K(+) channel AKT1 conducts K(+) uptake in root cells, and its activity is regulated by CBL1/9-CIPK23 complexes as well as by the AtKC1 channel subunit. CIPK23 and AtKC1 are both involved in the AKT1-mediated low-K(+) (LK) response; however, the relationship between them remains unclear. In this study, we screened suppressors of low-K(+) sensitive [lks1 (cipk23)] and isolated the suppressor of lks1 (sls1) mutant, which suppressed the leaf chlorosis phenotype of lks1 under LK conditions. Map-based cloning revealed a point mutation in AtKC1 of sls1 that led to an amino acid substitution (G322D) in the S6 region of AtKC1. The G322D substitution generated a gain-of-function mutation, AtKC1(D), that enhanced K(+) uptake capacity and LK tolerance in Arabidopsis. Structural prediction suggested that glycine-322 is highly conserved in K(+) channels and may function as the gating hinge of plant Shaker K(+) channels. Electrophysiological analyses revealed that, compared with wild-type AtKC1, AtKC1(D) showed enhanced inhibition of AKT1 activity and strongly reduced K(+) leakage through AKT1 under LK conditions. In addition, phenotype analysis revealed distinct phenotypes of lks1 and atkc1 mutants in different LK assays, but the lks1 atkc1 double mutant always showed a LK-sensitive phenotype similar to that of akt1 This study revealed a link between CIPK-mediated activation and AtKC1-mediated modification in AKT1 regulation. CIPK23 and AtKC1 exhibit distinct effects; however, they act synergistically and balance K(+) uptake/leakage to modulate AKT1-mediated LK responses in Arabidopsis.