ABSTRACT
Food allergy is becoming more frequent, with 6% of asthmatics reporting an isolated food allergy, and 5 to 6% of atopic dermatitis patients also have either a single or multiple true food allergy. There is value in immuno-biological diagnosis by: Measurement of total serum IgE. Measurement of mono-allergen-specific IgE, following a measurement by a multi-allergen of the Trophatope type. A study of elimination of foods for 2 or 3 months followed by their re-introduction. Oral provocation tests in a hospital environment under clinical control and subsequent measurement of the mediators:-Plasma histamine, tryptase, and urinary methylhistamine to give proof of responsibility of the food allergen. Nowadays, it is perfectly possible to include in diagnosis the new technologies of the test of activation of basophils/or lymphocytes by means of flow cytometry.
Subject(s)
Food Hypersensitivity/diagnosis , Adolescent , Adult , Aged , Animals , Antibody Specificity , Asthma, Exercise-Induced/etiology , Basophil Degranulation Test , Child , Child, Preschool , Dermatitis, Allergic Contact/complications , Diet , Eggs/adverse effects , Female , Food Hypersensitivity/complications , Food Hypersensitivity/diet therapy , Food Hypersensitivity/immunology , Humans , Immunoglobulin E/blood , Immunoglobulin E/immunology , Lymphocyte Activation , Male , Meat/adverse effects , Middle Aged , Nephrosis, Lipoid/etiology , Respiratory Hypersensitivity/complications , Skin Tests , Solanum tuberosum/adverse effects , SwineABSTRACT
With regard to an observation of anaphylaxis after a mosquito bite, we have shown by new immunobiological parameters and clinical investigations: Skin tests, specific IgE, Tests of Activation of basophils by flow cytometry (on basophils and total blood) and measurement of Leucotriene LTC4, the objective reality of the mechanism of IgE-dependent hypersensitivity (HS). This observation is correlated with rare work in support of true IgE-mediated allergy to mosquito 9 authors having used skin tests, PK in 1984 and immunoblot.
Subject(s)
Anaphylaxis/immunology , Culicidae/immunology , Insect Bites and Stings/immunology , Adult , Animals , Basophils/immunology , Humans , Immunoglobulin E/blood , Immunoglobulin E/immunology , Leukotriene C4/blood , Mice , Skin TestsABSTRACT
Food allergy is becoming more frequent, with 6% of asthmatics reporting an isolated food allergy, and 5 to 6% of atopic dermatitis patients also have either a single or multiple true food allergy. There is value in immuno-biological diagnosis by: Measurement of total serum IgE. Measurement of mono-allergen-specific IgE, following a measurement by a multi-allergen of the Trophatope type. A study of elimination of foods for 2 or 3 months followed by their re-introduction. Oral provocation tests in a hospital environment under clinical control and subsequent measurement of the mediators: Plasma histamine, tryptase, and urinary methylhistamine, to give proof of responsibility of the food allergen. Nowadays, it is perfectly possible to include in diagnosis the new technologies of the test of activation of basophils/or lymphocytes by means of flow cytometry.