Enhancing remediation efficiency of hyperaccumulators through earthworm addition: Evidence from a pot study on cadmium-contaminated soil.

Soil cadmium (Cd) contamination is an urgent environmental problem, which endangers human health through the food chain. Bioremediation attracted extensive attention around the world due to the high cost-efficiency. However, the remediation efficiency of different plant and earthworm species of soil Cd pollution is still unclear, it is thus of great significance to explore the combined effects of different remediation plants and earthworm species to improve the bioremediation capacity. In the present study, we consequently selected three species of Cd hyperaccumulator plants (vetiver, P. vittata and S. emarginatum) and three species of earthworms (E. fetida P1, E. fetida P2, and P. guillelmi) to compare the differences in Cd accumulation among various earthworm-plant combinations. Results indicated that the changes of soil pH and SOM in plant-animal combined application induced the higher soil Cd removal efficiency. The Cd removal efficiency showed highest in combination groups P. vittata-E. fetida P2 and P. vittata-P. guillelmi. Meanwhile, the improvements of biomass of plants and animals also were consistent with the increasing of Cd concentration in both plants and earthworms after combined application. It showed that the Cd concentrations in P. vittata were the highest while the TFs of Cd in S. emarginatum displays significantly more than that in others. In conclusion, the recommended combined system of earthworm-plant (P. vittata-E. fetida P2 and P. vittata-P. guillelmi) to provide reference for soil Cd bioremediation system in practice.

miR-940 modulates CD47 to suppress biological functions of lung adenocarcinoma cells.

OBJECTIVE: mir-940 and CD47 play regulatory and immunoregulatory roles in lung cancer. While previous study found that the expression of mir-940 decreased, associated with the increasing of CD47 in lung adenocarcinoma. However, their inherent correlations remain elusive. Herein, this experiment intends to search for the relevant molecular mechanisms regulating the biological function of non-small cell lung cancer. METHODS: The cancer and adjacent tissue samples were collected from 20 pairs of newly diagnosed non-small cell lung cancer patients without applying radiotherapy and chemotherapy. We performed immunohistochemistry containing 45 lung adenocarcinoma tissues to investigate the relationship between the clinicopathological features and CD47 expression. The expressions of mir-940 and CD47 were detected by real-time quantitative polymerase chain reaction (qRT-PCR). Lung epithelial and lung adenocarcinoma (A549, H1299, GLC-82, PC-9) cell lines were cultured to detect the expression of mir-940 and CD47 molecules in each cell line. According to the expression situation, 2 cell lines were selected for mimic and siRNA transfection, and the transfection efficiency was also verified by qRT-PCR and western blot. CCK-8, transwell migration, transwell invasion, and colony formation assays were used to detect the changes in biological functions of lung adenocarcinoma cells after transfection, such as enhanced proliferation, migration, invasion, and cloning. The changes of related protein molecules after transfection were detected by western blot. The dual-luciferase experiment verified the targeting regulation relationship between mir-940 and CD47. Finally, flow cytometry analysis of apoptosis and cell cycle were carried out to detect apoptosis cells and change phase of cell cycle distribution. RESULTS: CD47 expression was not associated with clinicopathologic factors in lung adenocarcinoma. The proliferation, migration, invasion, and cloning abilities of lung adenocarcinoma cells were weakened after transfection with mir-940 mimic and siRNA-CD47. Overexpression of CD47 could promote proliferation, migration, invasion, cloning abilities, reduce apoptosis rate and attenuate the antitumor effect of mir-940 on lung adenocarcinoma. Dual luciferase experiments confirmed that mir-940 can target CD47 molecules. CONCLUSION: mir-940 can inhibit the biological function of lung adenocarcinoma cells by targeting CD47.

Fate, abundance and ecological risks of microcystins in aquatic environment: The implication of microplastics.

Microcystins are highly toxic cyanotoxins and have been produced worldwide with the global expansion of harmful cyanobacterial blooms (HABs), posing serious threats to human health and ecosystem safety. Yet little knowledge is available on the underlying process occurring in the aquatic environment with microcystins. Microplastics as vectors for pollutants has received growing attention and are widely found co-existing with microcystins. On the one hand, microplastics could react with microcystins by adsorption, altering their environmental behavior and ecological risks. On the other hand, particular attention should be given to microplastics due to their implications on the outbreak of HABs and the generation and release of microcystins. However, limited reviews have been undertaken to link the co-existing microcystins and microplastics in natural water. This study aims to provide a comprehensive understanding on the environmental relevance of microcystins and microplastics and their potential interactions, with particular emphasis on the adsorption, transport, sources, ecotoxicity and environmental transformation of microcystins affected by microplastics. In addition, current knowledge gaps and future research directions on the microcystins and microplastics are presented. Overall, this review will provide novel insights into the ecological risk of microcystins associated with microplastics in real water environment and lay foundation for the effective management of HABs and microplastic pollution.

The synergistic triad of graphene quantum dots, polymer, and ferrites for the photodegradation of dyes in aqueous solution.

This work aimed to investigate photocatalytic properties of GQDs@PEG@Mg-ZnFe2O4 nanocomposite, composed of graphene quantum dots (GQDs), polyethylene glycol (PEG), and Mg-ZnFe2O4, for the degradation of methylene blue (MB) and crystal violet (CV). This nanocomposite was synthesized using facile ultrasonics-assisted methodology. XRD analysis confirmed the formation of the spinel structure of the Mg-ZnFe2O4 in the nanocomposite, whereas the presence of GQDs and PEG was confirmed by Fourier transform infrared spectroscopy. Scanning electron microscopy (SEM) revealed a reduction in agglomeration and particle size in the ternary nanocomposite. The GQDs@PEG@Mg-ZnFe2O4 nanocomposite demonstrates a remarkable degradation efficiency of 98 % for CV and MB dyes in the presence of sunlight in 120 min, indicating its potential as an efficient photocatalyst. Vibrating sample magnetometer (VSM) analysis confirmed the superparamagnetic behavior of the GQDs@PEG@Mg-ZnFe2O4 nanocomposite which enables magnetic recovery of the photocatalyst after the degradation process. Overall, this study emphasizes the utilization of an environmentally friendly approach to effectively eliminate organic pollutants from wastewater, addressing a crucial environmental concern.

Natural infochemical DMSP stimulates the transfer of microplastics from freshwater zooplankton to fish: An olfactory trap.

Natural infochemicals may largely affect the trophic transfer of microplastics (MPs) in ecosystems but such infochemical effect and mechanisms are poorly understood. Here, a daphnids-zebrafish freshwater microcosm was designed to elucidate whether and how an algae-derived infochemical, dimethylsulfoniopropionate (DMSP), affects the ingestion and transfer of MPs. Daphnids fast accumulated DMSP and MPs from water, and DMSP in daphnids was mainly enriched from the DMSP in water but not from MPs. DMSP did not change the MP ingestion by daphnids. A low concentration of DMSP (0.5 nM) increased predation of daphnids by zebrafish, while high concentrations of DMSP (50, 100 and 200 nM) did not increase predation rates. The concentration of DMSP in daphnids and the MP predation by zebrafish showed a unimodal relationship. The predation for MP by zebrafish in the 0.5 and 5 nM DMSP treatments was 1.89 and 1.56 times that of the control, respectively. The concentrations of DMSP in freshwater samples were lower than 50 nM. This suggests DMSP at environmentally relevant concentrations may promote the trophic transfer of MPs in freshwater ecosystems via olfactory traps.

Research progress on using biological cathodes in microbial fuel cells for the treatment of wastewater containing heavy metals.

Various types of electroactive microorganisms can be enriched to form biocathodes that reduce charge-transfer resistance, thereby accelerating electron transfer to heavy metal ions with high redox potentials in microbial fuel cells. Microorganisms acting as biocatalysts on a biocathode can reduce the energy required for heavy metal reduction, thereby enabling the biocathode to achieve a lower reduction onset potential. Thus, when such heavy metals replace oxygen as the electron acceptor, the valence state and morphology of the heavy metals change under the reduction effect of the biocathode, realizing the high-efficiency treatment of heavy metal wastewater. This study reviews the mechanisms, primary influencing factors (e.g., electrode material, initial concentration of heavy metals, pH, and electrode potential), and characteristics of the microbial community of biocathodes and discusses the electron distribution and competition between microbial electrodes and heavy metals (electron acceptors) in biocathodes. Biocathodes reduce the electrochemical overpotential in heavy metal reduction, permitting more electrons to be used. Our study will advance the scientific understanding of the electron transport mechanism of biocathodes and provide theoretical support for the use of biocathodes to purify heavy metal wastewater.

Erratum: Multivariate landscapes constructed by Bayesian estimation over five hundred microbial electrochemical time profiles.

[This corrects the article DOI: 10.1016/j.patter.2022.100610.].

Electroactive membrane fusion-liposome for increased electron transfer to enhance radiodynamic therapy.

Dynamic therapies have potential in cancer treatments but have limitations in efficiency and penetration depth. Here a membrane-integrated liposome (MIL) is created to coat titanium dioxide (TiO2) nanoparticles to enhance electron transfer and increase radical production under low-dose X-ray irradiation. The exoelectrogenic Shewanella oneidensis MR-1 microorganism presents an innate capability for extracellular electron transfer (EET). An EET-mimicking photocatalytic system is created by coating the TiO2 nanoparticles with the MIL, which significantly enhances superoxide anions generation under low-dose (1 Gy) X-ray activation. The c-type cytochromes-constructed electron channel in the membrane mimics electron transfer to surrounding oxygen. Moreover, the hole transport in the valence band is also observed for water oxidation to produce hydroxyl radicals. The TiO2@MIL system is demonstrated against orthotopic liver tumours in vivo.

Mechano-control of Extracellular Electron Transport Rate via Modification of Inter-heme Coupling in Bacterial Surface Cytochrome.

Bacterial outer-membrane multi-heme cytochromes (OMCs) mediate extracellular electron transport (EET). While heme alignment dictates the rate of EET, control of inter-heme coupling in a single OMC remains challenging, especially in intact cells. Given that OMCs diffuse and collide without aggregation on the cell surface, the overexpression of OMCs could increase such mechanical stress to impact the OMCs' protein structure. Here, the heme coupling is modified via mechanical interactions among OMCs by controlling their concentrations. Employment of whole-cell circular dichroism (CD) spectra of genetically engineered Escherichia coli reveals that the OMC concentration significantly impacts the molar CD and redox property of OMCs, resulting in a 4-fold change of microbial current production. The overexpression of OMCs increased the conductive current across the biofilm on an interdigitated electrode, indicating that a higher concentration of OMCs causes more lateral inter-protein electron hopping via collision on the cell surface. The present study would open a novel strategy to increase microbial current production by mechanically enhancing the inter-heme coupling.

Stimulation of atrazine degradation by activated carbon and cathodic effect in soil microbial fuel cell.

Soil microbial fuel cells (MFCs) have been increasingly studied in recent years and have attracted significant attention as an environmentally sustainable bioelectrochemical technology. However, the poor conductivity of the soil matrix and the neglect of the cathodic function have limited its application. In this study, quartz sand and activated carbon were subjected to investigation on their influence on atrazine degradation. Atrazine was introduced in different layers (cathode, upper layer) to explore the cathodic effect on atrazine removal. The results revealed that activated carbon could reduce the internal resistance (693 Ω) and generate the highest power density (25.51 mW/m2) of the soil MFCs, and thus increase the removal efficiency (97.92%) of atrazine. The dynamic degradation profiles of atrazine were different for different adding layers. The cathode electrode acted as an electron donor could increase the distance of the effective influence of the soil MFCs' cathode from the middle to the cathode layer. The cathode (region) and the region close to the cathode could degrade atrazine with the atrazine removal efficiencies ranging from 60.67% to 92.79%, and the degradation ability of the cathode was stronger than that of other layers. The degradation effect followed the order: cathode > upper > lower > middle). Geobacter, Desulfobulbus, and Desulfuromonas belonging to the δ-Proteobacteria class were identified as the dominant electroactive microorganisms in the anode layer, while their relative abundances are quite low in the upper and cathode layers. Pseudomonas is an atrazine-degrading bacterium, but its relative abundance was only 0.13-0.51%. Thus, bioelectrochemistry rather than microbial degradation was the primary driving force.

Reusable and Practical Biocomposite Based on Sphingopyxis sp. YF1 and Polyacrylonitrile-Based Carbon Fiber for the Efficient Bioremediation of Microcystin-LR-Contaminated Water.

Microbial degradation is a cost-effective and environmentally friendly method for removing microcystin-LR (MC-LR). However, the application of free bacteria has limitations due to low operational stability and difficulties in recovery. In a previous study, our group successfully isolated a highly efficient MC-LR-degrading bacterium, Sphingopyxis sp. YF1, from Taihu. To enhance its practical potential in addressing MC-LR-contaminated water pollution, a novel biological material named polyacrylonitrile-based carbon fiber @Sphingopyxis sp. YF1 (PAN-CF@YF1) was synthesized. The immobilization conditions of strain Sphingopyxis sp. YF1 on PAN-CF surfaces were optimized using Box-Behnken design and response surface methodology (RSM), which turned out to be an optimal pH of 7.6 for the culture medium, a ratio of 0.038 g of supporting materials per 100 mL of culture media, and an incubation time of 53.4 h. The resultant PAN-CF@YF1 showed a great degradation effect both for low and high concentrations of MC-LR and exhibited satisfactory cyclic stability (85.75% after six cycles). Moreover, the application of PAN-CF@YF1 in the bioreactors demonstrated effective and sustainable MC-LR removal, with a removal efficiency of 78.83% after three consecutive treatments. Therefore, PAN-CF@YF1 with high degradation activity, environmental compatibility, straightforward preparation, and recyclability shows significant application potential for the bioremediation of MC-LR-contaminated water bodies.

Multivariate landscapes constructed by Bayesian estimation over five hundred microbial electrochemical time profiles.

Data science emerges as a promising approach for studying and optimizing complex multivariable phenomena, such as the interaction between microorganisms and electrodes. However, there have been limited reports on a bioelectrochemical system that can produce a reliable database until date. Herein, we developed a high-throughput platform with low deviation to apply two-dimensional (2D) Bayesian estimation for electrode potential and redox-active additive concentration to optimize microbial current production (I c ). A 96-channel potentiostat represents <10% SD for maximum I c . 576 time-I c profiles were obtained in 120 different electrolyte and potentiostatic conditions with two model electrogenic bacteria, Shewanella and Geobacter. Acquisition functions showed the highest performance per concentration for riboflavin over a wide potential range in Shewanella. The underlying mechanism was validated by electrochemical analysis with mutant strains lacking outer-membrane redox enzymes. We anticipate that the combination of data science and high-throughput electrochemistry will greatly accelerate a breakthrough for bioelectrochemical technologies.

Advance in remediated of heavy metals by soil microbial fuel cells: Mechanism and application.

In the past decade, studies on the remediation of heavy metals contaminated soil by microbial fuel cells (MFCs) have attracted broad attention because of the self-generated power and their multifield principles such as the extracellular electron transfer (EET) reduction, electromigration for heavy metals removal. However, given the bio electro-motive power from soil MFCs is weak and fluctuated during the remediation, we need to comprehensively understand the origination of driving force in MFC based on the analysis of the fundamental rationale of ion moving in cells and improve the performance via the appropriate configurations and operations. In this review, we first described the structures of soil MFCs for heavy metals remediation and compared the advantages of different types of configurations. Then, based on the theoretical models of heavy metal migration, enrichment, and reduction in soil MFCs, the optimization of soil MFCs including the length of the remediation area, soil conductivity, control of electrode reaction, and modification of electrodes were proposed. Accordingly, this review contributes to the application of bioelectrochemistry to efficiently remove heavy metals from soils.

Riboflavin-rich agar enhances the rate of extracellular electron transfer from electrogenic bacteria inside a thin-layer system.

Numerous bacteria owe extracellular electron transport (EET) ability, and the rate enhancement of EET is critical for the emerging sensor technology to detect metabolically active pathogens. Here, the considerable enhancement of microbial current signal was firstly demonstrated in a thin layer electrolyte sandwiched between an agar substrate (AS) containing high concentration riboflavin (RF) and a screen-printed electrode. Covering cells with this AS showed a sharply current increase from 0.033 µA to 1.59 µA (47.7-folds) in EET-capable bacteria Shewanella oneidensis MR-1. Differential pulse voltammograms using gene-deletion mutant strains of S. oneidensis MR-1 revealed thin electrolyte between RF-loaded AS and electrode enhanced the rate of electron transfer via complexes between riboflavin and outer membrane c-type cytochrome. A similar effect in Streptococcus mutans UA159, a biofilm-forming pathogen, was also explored. Moreover, capturing and quantifying both metabolically active microbes from the dry solid surface are demonstrated with RF-loaded AS successfully. The considerable enhancement of the EET in the thin layer electrolyte provides a new direction for designing whole-cell biosensors and understanding a microbe/electrode interaction in a micro-sized space.

Electrochemical impedance spectroscopy applied to microbial fuel cells: A review.

Electrochemical impedance spectroscopy (EIS) is an efficient and non-destructive test for analyzing the bioelectrochemical processes of microbial fuel cells (MFCs). The key factors limiting the output performance of an MFC can be identified by quantifying the contribution of its various internal parts to the total impedance. However, little attention has been paid to the measurement conditions and diagrammatic processes of the EIS for MFC. This review, starting with the analysis of admittance of bioelectrode, introduces conditions for the EIS measurement and summarizes the representative equivalent circuit plots for MFC. Despite the impedance from electron transfer and diffusion process, the effect of unnoticeable capacitance obtained from the Nyquist plot on MFCs performance is evaluated. Furthermore, given that distribution of relaxation times (DRT) is an emerging method for deconvoluting EIS data in the field of fuel cell, the application of DRT-analysis to MFC is reviewed here to get insight into bioelectrode reactions and monitor the biofilm formation. Generally, EIS measurement is expected to optimize the construction and compositions of MFCs to overcome the low power generation.

Current Production Capability of Drug-Resistant Pathogen Enables Its Rapid Label-Free Detection Applicable to Wastewater-Based Epidemiology.

A rapid and label-free method for the detection of drug-resistant pathogens is in high demand for wastewater-based epidemiology. As recently shown, the extent of electrical current production (Ic) is a useful indicator of a pathogen's metabolic activity. Therefore, if drug-resistant bacteria have extracellular electron transport (EET) capability, a simple electric sensor may be able to detect not only the growth as a conventional plating technique but also metabolic activity specific for drug-resistant bacteria in the presence of antibiotics. Here, one of the multidrug-resistant pathogens in wastewater, Klebsiella pneumoniae, was shown to generate Ic, and the extent of Ic was unaffected by the microbial growth inhibitor, kanamycin, while the current was markedly decreased in environmental EET bacteria Shewanella oneidensis. Kanamycin differentiated Ic in K. pneumonia and S. oneidensis within 3 h. Furthermore, the detection of K. pneumoniae was successful in the presence of S. oneidensis in the electrochemical cell. These results clarify the advantage of detecting drug-resistant bacteria using whole-cell electrochemistry as a simple and rapid method to detect on-site drug-resistant pathogens in wastewater, compared with conventional colony counting, which takes a few days.

Outer membrane compositions enhance the rate of extracellular electron transport via cell-surface MtrC protein in Shewanella oneidensis MR-1.

While cell membrane composition is critical for the function of membrane proteins, membrane modification has not been used to control the rate of extracellular electron transfer (EET) via the outer membrane protein complexes. Here, the rate of electron flow via the cell-surface redox protein, MtrC, was highly enhanced upon change in the outer membrane composition in Shewanella oneidensis MR-1. The MR-1 strain was pre-cultured at 4 °C and 30 °C to initiate differentiation of membrane composition. The whole-cell difference electrochemical assay of wild-type and mutant strains lacking MtrC suggested that the rate of EET via MtrC increased approximately 18 times at 4 °C than 30 °C. Circular dichroism spectroscopy showed that the molar exciton coupling coefficient for inter-heme interaction in MtrC increased in MR-1 at 4 °C than 30 °C. Results suggest that membrane modification may be a novel strategy for improving the efficiency of EET-based technologies.

Limitation of voltage reversal in the degradation of azo dye by a stacked double-anode microbial fuel cell and characterization of the microbial community structure.

In this study, two double-anode microbial fuel cells (MFCs) were connected in series for degradation of the azo dye reactive brilliant red X-3B. After the series connection, the electricity generation of one of the MFCs decreased, and the other was not affected too much. Due to the special structure in the double-anode MFC reduced the imbalanced performance between the MFC units, the occurrence of voltage reversal was limited. The removal efficiencies in two MFC reactors were not consistent after the series connection, the results showed that the MFC with the reduced electricity generation had the higher removal efficiencies, it was 12.90, 11.66, and 40.05% higher than in the MFC in which the power generation capacity was not affected after the series connection, the MFC without serial connection, and the control group, respectively. Meanwhile, the microbial communities related to the degradation of refractory organic compounds increased and related to electricity generation decreased in the MFC with the reduced electricity generation, the changes of the microbial communities were consistent with its electricity generation and the removal efficiencies. The degradation products in the effluent from two MFC units showed that had the products generated from the MFC with the reduced electricity generation had simpler structures comparing the other MFC unit.

Simultaneous enhancement of heavy metal removal and electricity generation in soil microbial fuel cell.

As an environmentally sustainable bioelectrochemical technology, the microbial fuel cell (MFC) has attracted great attention. In this study, a three-chamber MFC (TC-MFC) was enhanced with different auxiliary reagents to remove heavy metals from soil. The results showed that the removal efficiency of heavy metals from soil increased with increasing auxiliary reagent concentration. When 1 mol/L citric acid, HCl, or acetic acid were used as an auxiliary reagent, the total copper (500 mg/kg) removal efficiency after 74 days of TC-MFC treatment was 3.89, 5.01 and 2.01 times that of the control group, respectively. The highest soil electrical conductivity (15.29 ms/cm), ionic heavy metal content (94.78%), electricity generation performance (363.04 mW h), and desorption stability of heavy metals were obtained when using 1 mol/L HCl as an auxiliary reagent, indicating that HCl was more suitable for the remediation of heavy metals in soil using a TC-MFC. Correlation analysis showed that the electricity generation of the TC-MFC was linearly related to the removal efficiency of heavy metals from soil (R2 = 0.9296). At the same time, higher content of ionic heavy metals in the soil led to better migration of heavy metals under the internal electric field of the TC-MFC.

The synergistic effect of biophoto anode for the enhancement of current generation and degradation.

The demand for removal of refractory organic pollutants limits the application of microbial fuel cells. In this study, the synergistic effects of bioelectrochemical and photocatalysis methods were captured by constructing a biophoto anode from a combination of WO3/TiO2 and carbon felt. This biophoto electrode was able to decrease the aniline concentration from 63.3 ± 6.2 to 9.3 ± 5.5 mg/L. The structure of the benzene ring was broken through strong oxidation by photocatalysis. Electrochemical analysis showed that photocatalysis also enhanced the extracellular electron transfer of microorganisms and reduced the resistance of the anode from 136.9â€…Ω to 69.9 Ω. In addition, the maximum current output increased by 28.5% under the composite biophoto electrode. Further analysis of the microbial community indicated that the biophoto electrode promoted the enrichment of Geobacter in the anode. This biophoto electrode provided a method for overcoming the disadvantages of anaerobic degradation of refractory organics.