Exploring atmospheric optical turbulence: observations across zenith angles.

We present measurements of the atmospheric optical turbulence as a function of zenith angle using two identical instruments, Shack-Hartmann Image Motion Monitors (SHIMMs), to measure atmospheric parameters concurrently. One instrument was pointed near zenith, while the other collected data by tracking a single star until it set and thus sampling zenith angles continuously to the horizon. By comparing these measurements, we can attribute changes in the atmospheric parameters to the changing zenith angle rather than variations in local turbulence conditions. The primary purpose of this experiment is to make comparisons between the measurements of the scintillation index, σ I2, and Fried parameter, r 0, with current theories. In this demonstration, we find that there is a strong agreement between the models and the instrument up until zenith angles of 70∘, above which model and measurements begin to deviate. We discuss various ways in which limitations in models and our instrument may cause these deviations.

Paleoecology and paleoceanography of the Athel silicilyte, Ediacaran-Cambrian boundary, Sultanate of Oman.

The Athel silicilyte is an enigmatic, hundreds of meters thick, finely laminated quartz deposit, in which silica precipitated in deep water (>~100-200 m) at the Ediacaran-Cambrian boundary in the South Oman Salt Basin. In contrast, Meso-Neoproterozoic sinks for marine silica were dominantly restricted to peritidal settings. The silicilyte is known to contain sterane biomarkers for demosponges, which today are benthic, obligately aerobic organisms. However, the basin has previously been described as permanently sulfidic and time-equivalent shallow-water carbonate platform and evaporitic facies lack silica. The Athel silicilyte thus represents a unique and poorly understood depositional system with implications for late Ediacaran marine chemistry and paleoecology. To address these issues, we made petrographic observations, analyzed biomarkers in the solvent-extractable bitumen, and measured whole-rock iron speciation and oxygen and silicon isotopes. These data indicate that the silicilyte is a distinct rock type both in its sedimentology and geochemistry and in the original biology present as compared to other facies from the same time period in Oman. The depositional environment of the silicilyte, as compared to the bounding shales, appears to have been more reducing at depth in sediments and possibly bottom waters with a significantly different biological community contributing to the preserved biomarkers. We propose a conceptual model for this system in which deeper, nutrient-rich waters mixed with surface seawater via episodic mixing, which stimulated primary production. The silica nucleated on this organic matter and then sank to the seafloor, forming the silicilyte in a sediment-starved system. We propose that the silicilyte may represent a type of environment that existed elsewhere during the Neoproterozoic. These environments may have represented an important locus for silica removal from the oceans.

Methane sources in arctic thermokarst lake sediments on the North Slope of Alaska.

The permafrost on the North Slope of Alaska is densely populated by shallow lakes that result from thermokarst erosion. These lakes release methane (CH4 ) derived from a combination of ancient thermogenic pools and contemporary biogenic production. Despite the potential importance of CH4 as a greenhouse gas, the contribution of biogenic CH4 production in arctic thermokarst lakes in Alaska is not currently well understood. To further advance our knowledge of CH4 dynamics in these lakes, we focused our study on (i) the potential for microbial CH4 production in lake sediments, (ii) the role of sediment geochemistry in controlling biogenic CH4 production, and (iii) the temperature dependence of this process. Sediment cores were collected from one site in Siqlukaq Lake and two sites in Sukok Lake in late October to early November. Analyses of pore water geochemistry, sedimentary organic matter and lipid biomarkers, stable carbon isotopes, results from CH4 production experiments, and copy number of a methanogenic pathway-specific gene (mcrA) indicated the existence of different sources of CH4 in each of the lakes chosen for the study. Analysis of this integrated data set revealed that there is biological CH4 production in Siqlukaq at moderate levels, while the very low levels of CH4 detected in Sukok had a mixed origin, with little to no biological CH4 production. Furthermore, methanogenic archaea exhibited temperature-dependent use of in situ substrates for methanogenesis, and the amount of CH4 produced was directly related to the amount of labile organic matter in the sediments. This study constitutes an important first step in better understanding the actual contribution of biogenic CH4 from thermokarst lakes on the coastal plain of Alaska to the current CH4 budgets.

Quantitative high dynamic range beam profiling for fluorescence microscopy.

Modern developmental biology relies on optically sectioning fluorescence microscope techniques to produce non-destructive in vivo images of developing specimens at high resolution in three dimensions. As optimal performance of these techniques is reliant on the three-dimensional (3D) intensity profile of the illumination employed, the ability to directly record and analyze these profiles is of great use to the fluorescence microscopist or instrument builder. Though excitation beam profiles can be measured indirectly using a sample of fluorescent beads and recording the emission along the microscope detection path, we demonstrate an alternative approach where a miniature camera sensor is used directly within the illumination beam. Measurements taken using our approach are solely concerned with the illumination optics as the detection optics are not involved. We present a miniature beam profiling device and high dynamic range flux reconstruction algorithm that together are capable of accurately reproducing quantitative 3D flux maps over a large focal volume. Performance of this beam profiling system is verified within an optical test bench and demonstrated for fluorescence microscopy by profiling the low NA illumination beam of a single plane illumination microscope. The generality and success of this approach showcases a widely flexible beam amplitude diagnostic tool for use within the life sciences.

Traceable interferometry using binary reconfigurable holograms.

We describe the characterization of a ferroelectric-liquid-crystal-on-silicon (FLCOS) spatial light modulator (SLM) in the production of holograms for use in interferometric metrology. It has already been shown that such a device can be used in producing small-amplitude arbitrary reference surfaces with small but appreciable errors due to the contaminating effect of higher-order structures being propagated through the spatial filter. Here we further quantify the size of these residuals for increasingly large aberrations up to nine waves rms Zernike astigmatism, showing a Zernike-corrected rms wavefront error of roughly 0.06 waves with high vibrational stability. We also present measurements of a vacuum window element using the FLCOS device to drastically reduce interferometric fringe density, showing a residual wavefront error of 0.046 waves rms with dominant components originating from test piece structure rather than holographic errors.

Modal liquid crystal array of optical elements.

In this study, a novel liquid crystal array based on modal control principle is proposed and demonstrated. The advanced device comprises a six striped electrode structure that forms a configurable 2D matrix of optical elements. A simulation program based on the Frank-Oseen equations and modal control theory has been developed to predict the device electrooptic response, that is, voltage distribution, interference pattern and unwrapped phase. A low-power electronics circuit, that generates complex waveforms, has been built for driving the device. A combined variation of the waveform amplitude and phase has provided a high tuning versatility to the device. Thus, the simulations have demonstrated the generation of a liquid crystal prism array with tunable slope. The proposed device has also been configured as an axicon array. Test measurements have allowed us to demonstrate that electrooptic responses, simulated and empirical, are fairly in agreement.

Carbon isotopes and lipid biomarkers from organic-rich facies of the Shuram Formation, Sultanate of Oman.

The largest recorded carbon isotopic excursion in Earth history is observed globally in carbonate rocks of middle Ediacaran age. Known from the Sultanate of Oman as the 'Shuram excursion', this event records a dramatic, systematic shift in δ(13) Ccarbonate values to ca. -12‰. Attempts to explain the nature, magnitude and origin of this excursion include (i) a primary signal resulting from the protracted oxidation of a large dissolved organic carbon reservoir in seawater, release of methane from sediment-hosted clathrates, or water column stratification; and (ii) a secondary signal from diagenetic processes. The compositions and isotope ratios of organic carbon phases during the excursion are critical to evaluating these ideas; however, previous work has focused on localities that are low in organic carbon, hindering straightforward interpretation of the observed time-series trends. We report carbon isotope data from bulk organic carbon, extracted bitumen and kerogen, in addition to lipid biomarker data, from a subsurface well drilled on the eastern flank of the South Oman Salt Basin, Sultanate of Oman. This section captures Nafun Group strata through the Ediacaran-Cambrian boundary in the Ara Group and includes an organic-rich, deeper-water facies of the Shuram Formation. Despite the high organic matter contents, the carbon isotopic compositions of carbonates - which record a negative δ(13) C isotope excursion similar in shape and magnitude to sections elsewhere in Oman - do not covary with those of organic phases (bulk TOC, bitumen and kerogen). Paired inorganic and organic δ(13) C data only display coupled behaviour during the latter part of the excursion's recovery. Furthermore, lipid biomarker data reveal that organic matter composition and source inputs varied stratigraphically, reflecting biological community shifts in non-migrated, syngenetic organic matter deposited during this interval.

Optical binding mechanisms: a conceptual model for Gaussian beam traps.

Optical binding interactions between laser-trapped spherical microparticles are familiar in a wide range of trapping configurations. Recently it has been demonstrated that these experiments can be accurately modeled using Mie scattering or coupled dipole models. This can help confirm the physical phenomena underlying the inter-particle interactions, but does not necessarily develop a conceptual understanding of the effects that can lead to future predictions. Here we interpret results from a Mie scattering model to obtain a physical description which predict the behavior and trends for chains of trapped particles in Gaussian beam traps. In particular, it describes the non-uniform particle spacing and how it changes with the number of particles. We go further than simply demonstrating agreement, by showing that the mechanisms "hidden" within a mathematically and computationally demanding Mie scattering description can be explained in easily-understood terms.

Everybody's talking: using entertainment-education video to reduce barriers to discussion of cervical cancer screening among Thai women.

Although Southeast Asian women are at exceedingly high risk for cervical cancer, low rates of the Pap testing necessary for early detection and successful treatment continue among this group. Previous research suggests that discussions about Pap testing with important people in a woman's life, particularly her doctor, may increase the likelihood of screening; therefore increasing women's discussions about cancer screenings is an important step toward behavior change. The purpose of this study was to determine the effectiveness of a culturally sensitive, seven-minute video intervention in reducing barriers to discussions about Pap tests among Thai women. This unique video presented Thai actors, speaking in Thai, in a soap opera format. Participants completed a self-report questionnaire at baseline, immediately after the intervention and at 3-month follow-up. The comparison group received an educational pamphlet. Although the results indicated that both groups experienced reductions in barriers to communicating with others about Pap tests, the intervention group had significantly stronger outcomes than the comparison group for communicating about Pap tests in general as well as to doctors. These findings suggest that intermediate communication effects such as self-efficacy, collective efficacy and perhaps interpersonal communication may reduce barriers to discussion and positive decision making regarding Pap tests.

Multipole expansion of Bessel and Gaussian beams for Mie scattering calculations.

Multipole expansions of Bessel and Gaussian beams, suitable for use in Mie scattering calculations, are derived. These results allow Mie scattering calculations to be carried out considerably faster than existing methods, something that is of particular interest for time evolution simulations where large numbers of scattering calculations must be performed. An analytic result is derived for the Bessel beam that improves on a previously published expression requiring the evaluation of an integral. An analogous expression containing a single integral, similar to existing results quoted, but not derived, in literature, is derived for a Gaussian beam, valid from the paraxial limit all the way to arbitrarily high numerical apertures.

Emergent properties in optically bound matter.

Sub-micron particles have been observed to spontaneously form regular two-dimensional structures in counterpropagating evanescent laser fields. We show that collective properties of large numbers of optically-trapped particles can be qualitatively different to the properties of small numbers. This is demonstrated both with a computer model and with experimental results. As the number of particles in the structure is increased, optical binding forces can be sufficiently large to overcome the optical landscape imposed by the interference fringes of the laser beams and impose a different, competing structure.

Colloidal interactions and transport in nematic liquid crystals.

We describe a new nematic liquid-crystal colloid system which is characterized by both charge stabilization of the particles and an interaction force. We estimate the effective charge of the particles by electrophoretic measurements and find that in such systems the director anchoring energy W is very low and the particles have little director distortion around them. The interaction force is created by producing a radial distribution of the nematic order parameter around a locally isotropic region created by ir laser heating. We theoretically describe this as being due to the induced flexoelectric polarization, the quadrupolar symmetry of which provides the required long-range force acting on charged particles.

Eigenmodes of a hydrodynamically coupled micron-size multiple-particle ring.

We use a continuous acquisition, high-speed camera with integrated centroid tracking to simultaneously measure the positions of a ring of micron-sized particles held in holographic optical tweezers. Hydrodynamic coupling between the particles gives a set of eigenmodes, each one independently relaxing with a characteristic decay rate (eigenvalue) that can be measured using our positional data. Despite the finite particle size, we find an excellent agreement between the measured eigenvalues and those numerically predicted by Oseen theory applied to the two-dimensional (2D) ring geometry. Particle motions are also analyzed in terms of the alternative eigenmode set obtained by wrapping onto the ring the eigenmodes of a 1D periodic chain. We identify the modes for which the periodic chain is a good approximation to the ring and those for which it is not.

Tuneable planar integrated optical systems.

Planar integrated free-space optical systems are well suited for a variety of applications, such as optical interconnects and security devices. Here, we demonstrate for the first time dynamic functionality of such microoptical systems by the integration of adaptive liquid-crystal-devices.

Laser beacon wave-front sensing without focal anisoplanatism.

Wave-front sensing from artificial beacons is normally performed by formation of a focused spot in the atmosphere and sensing of the wave-front distortions produced during the beam's return passage. We propose an alternative method that senses the distortions produced during the outgoing path by forming an intensity pattern in the atmosphere that is then viewed from the ground. A key advantage of this method is that a parallel beam is used, and therefore the wave-front measurements will not suffer from the effects of focal anisoplanatism. We also envisage other geometries, all based on the concept of projecting a pupil pattern onto the atmosphere.

White-light performance of a polarization-independent liquid-crystal phase modulator.

The broadband performance of a polarization-insensitive liquid-crystal phase modulator is analyzed, and its effect on an adaptive optics system is quantified.

Real-time optical aberration correction with a ferroelectric liquid-crystal spatial light modulator.

Real-time correction of an optically aberrated wave front by use of a 10 x 10 ferroelectric liquid-crystal spatial light modulator as the correction device and a point-diffraction interferometer as the wave-front sensor is demonstrated. This type of interferometer requires no reference arm and so can be used, in theory, in an astronomical adaptive-optics system. We discuss some of the unusual features of the point-diffraction interferometer for wave-front sensing.

Wave-front correction and production of Zernike modes with a liquid-crystal spatial light modulator.

I describe a nematic liquid-crystal spatial light modulator that can be used as a high-precision wave-front control device. I present results showing the open-loop correction of wave-front aberrations and demonstrate wave-front shaping by the production and quantification of the first 15 significant Zernike terms.

Intensity-only modulation for atmospheric scintillation correction by liquid-crystal spatial light modulators.

We consider the use of a ferroelectric liquid-crystal spatial light modulator (FLC SLM) to control the transmittance of a telescope pupil to compensate for the effects of scintillation. Our aim here is to prove the necessary and physically interesting result that it is possible to control the intensity of light by use of FLC SLM without inducing further phase aberrations. Furthermore, we show that system errors have only a small effect on the phase of the transmitted beam.