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1.
Oncogene ; 30(16): 1923-35, 2011 Apr 21.
Article in English | MEDLINE | ID: mdl-21217778

ABSTRACT

The critical 8p22 tumor suppressor deleted in liver cancer 1 (DLC1) is frequently inactivated by aberrant CpG methylation and/or genetic deletion and implicated in tumorigeneses of multiple tumor types. Here, we report the identification and characterization of its new isoform, DLC1 isoform 4 (DLC1-i4). This novel isoform encodes an 1125-aa (amino acid) protein with distinct N-terminus as compared with other known DLC1 isoforms. Similar to other isoforms, DLC1-i4 is expressed ubiquitously in normal tissues and immortalized normal epithelial cells, suggesting a role as a major DLC1 transcript. However, differential expression of the four DLC1 isoforms is found in tumor cell lines: Isoform 1 (longest) and 3 (short thus probably nonfunctional) share a promoter and are silenced in almost all cancer and immortalized cell lines, whereas isoform 2 and 4 utilize different promoters and are frequently downregulated. DLC1-i4 is significantly downregulated in multiple carcinoma cell lines, including 2/4 nasopharyngeal, 8/16 (50%) esophageal, 4/16 (25%) gastric, 6/9 (67%) breast, 3/4 colorectal, 4/4 cervical and 2/8(25%) lung carcinoma cell lines. The functional DLC1-i4 promoter is within a CpG island and is activated by wild-type p53. CpG methylation of the DLC1-i4 promoter is associated with its silencing in tumor cells and was detected in 38-100% of multiple primary tumors. Treatment with 5-aza-2'-deoxycytidine or genetic double knockout of DNMT1 and DNMT3B led to demethylation of the promoter and reactivation of its expression, indicating a predominantly epigenetic mechanism of silencing. Ectopic expression of DLC1-i4 in silenced tumor cells strongly inhibited their growth and colony formation. Thus, we identified a new isoform of DLC1 with tumor suppressive function. The differential expression of various DLC1 isoforms suggests interplay in modulating the complex activities of DLC1 during carcinogenesis.


Subject(s)
Chromosomes, Human, Pair 8 , GTPase-Activating Proteins/genetics , Genes, Tumor Suppressor , Neoplasms/pathology , Tumor Suppressor Proteins/genetics , Base Sequence , DNA Methylation , DNA Primers , Gene Silencing , Humans , Molecular Sequence Data , Neoplasms/genetics
2.
Oncogene ; 26(6): 934-44, 2007 Feb 08.
Article in English | MEDLINE | ID: mdl-16862168

ABSTRACT

Identification of tumor suppressor genes (TSG) silenced by methylation uncovers mechanisms of tumorigenesis and identifies new epigenetic tumor markers for early cancer detection. Both nasopharyngeal carcinoma (NPC) and esophageal carcinoma are major tumors in Southern China and Southeast Asia. Through expression subtraction of NPC, we identified Deleted in Liver Cancer 1 (DLC1)/ARHGAP7 (NM_006094)--an 8p22 TSG as a major downregulated gene. Although expressed in all normal tissues, DLC1 was silenced or downregulated in 11/12 (91%) NPC, 6/15 (40%) esophageal, 5/8 (63%) cervical and 3/9 (33%) breast carcinoma cell lines. No genetic deletion of DLC1 was detected in NPC although a hemizygous deletion at 8p22-11 was found by 1-Mb array-CGH in some cell lines. We then located the functional DLC1 promoter by 5'-RACE and promoter activity assays. This promoter was frequently methylated in all downregulated cell lines and in a large collection of primary tumors including 89% (64/72) NPC (endemic and sporadic types), 51% (48/94) esophageal, 87% (7/8) cervical and 36% (5/14) breast carcinomas, but seldom in paired surgical marginal tissues and not in any normal epithelial tissue. The transcriptional silencing of DLC1 could be reversed by 5-aza-2'-deoxycytidine or genetic double knock-out of DNMT1 and DNMT3B. Furthermore, ectopic expression of DLC1 in NPC and esophageal carcinoma cells strongly inhibited their colony formation. We thus found frequent epigenetic silencing of DLC1 in NPC, esophageal and cervical carcinomas, and a high correlation of methylation with its downregulation, suggesting a predominant role of epigenetic inactivation. DLC1 appears to be a major TSG implicated in the pathogenesis of these tumors, and should be further tested as a molecular biomarker in patients with these cancers.


Subject(s)
DNA Methylation , Esophageal Neoplasms/genetics , Gene Expression Regulation, Neoplastic , Gene Silencing , Nasopharyngeal Neoplasms/genetics , Nasopharyngeal Neoplasms/pathology , Tumor Suppressor Proteins/genetics , Uterine Cervical Neoplasms/genetics , Base Sequence , Cell Line , Cell Line, Tumor , Cell Proliferation , Chromosomes, Human, Pair 8/genetics , Esophageal Neoplasms/metabolism , Female , GTPase-Activating Proteins , Humans , Molecular Sequence Data , Nasopharyngeal Neoplasms/metabolism , Promoter Regions, Genetic/genetics , Tumor Suppressor Proteins/metabolism , Uterine Cervical Neoplasms/metabolism
3.
Ann Oncol ; 17(11): 1625-30, 2006 Nov.
Article in English | MEDLINE | ID: mdl-17008411

ABSTRACT

BACKGROUND: Celecoxib is a selective cyclooxygenase-2 inhibitor with antitumor and antiangiogenic activity. We sought to determine pharmacodynamic change in tumors of patients with nasopharyngeal carcinoma (NPC) treated with celecoxib. METHODS: Tumor biopsies were obtained before and after treatment with celecoxib 400 mg b.i.d. for 14 days in patients with newly diagnosed, untreated NPC. Tumor angiogenesis and cell proliferation were assessed by immunohistochemistry and gene expression by microarray analysis. Plasma celecoxib concentrations were obtained on days 8 and 14. RESULTS: Paired samples were analyzed in 15 patients. Microvessel density was reduced in post-treatment samples and mean celecoxib levels reached therapeutic levels. Thirty-five genes (27 down-regulated, eight up-regulated) were differentially expressed on microarray analysis (p < 0.001). Down-regulated genes included cell cycle regulation-related (cyclin-dependent kinase 2, YES1), transcription factor (TRIP-Br2), whereas the antigen processing and presentation-related gene HLA-DM B was up-regulated. CONCLUSION: Celecoxib reduced angiogenesis and induced tumor transcriptional changes. Further characterization of these transcriptional changes in vivo is needed to provide further insights into the effects of celecoxib in neoplastic tissue. Our findings provide a rationale for clinical studies aimed at assessing the efficacy of celecoxib in the treatment of NPC.


Subject(s)
Antineoplastic Agents/pharmacology , Blood Vessels/drug effects , Gene Expression Regulation, Neoplastic/drug effects , Nasopharyngeal Neoplasms/drug therapy , Pyrazoles/pharmacology , Sulfonamides/pharmacology , Adult , Aged , Antineoplastic Agents/blood , Antineoplastic Agents/therapeutic use , Celecoxib , Cyclooxygenase 2/metabolism , Female , Genes, Neoplasm , Humans , Immunohistochemistry , Ki-67 Antigen/metabolism , Male , Middle Aged , Nasopharyngeal Neoplasms/genetics , Pyrazoles/blood , Pyrazoles/therapeutic use , Sulfonamides/blood , Sulfonamides/therapeutic use , Transcription, Genetic/drug effects
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