Potential availability of metals in anaerobic mono- and co-digestion of pig manure and maize.

This study aims to analyse the potential availability of essential metals including as Co, Fe, Ni, Zn, Mn, and Cu and non-essential metals such as Pb, Cr, and Cd within anaerobic mono- and co-digestion of pig manure and maize. The metals partitioning was determined using the Modified BCR (European Community Bureau of Reference) sequential extraction at defined intervals over a 45-days period, correlating changes in metals speciation with key digestion variables. The findings revealed that Cr, Cu, Fe, and Pb were predominantly associated with the oxidisable fraction, while Zn, Mn, and Cd were potentially available in both processes. Notably, NH4+-N and the VFAs, except propionic acid, correlated significantly with the available fractions of Co, Mn, Ni, Zn, Cr, and Pb during mono-digestion of pig manure. The wider pH range and the chemical properties of the feedstock in co-digestion resulted in varied correlations between the metals availability and the digestion variables.

Development of conversion factors to estimate the concentrations of heavy metals in manure-derived digestates.

During biogas production, a residual by-product rich in organic matter, nutrients, and trace elements - called digestate - is generated. Due to the nature of the anaerobic digestion process (i.e., conversion of organic matter into biogas) and the non-digestibility of trace elements, metal concentrations are higher in digestate than initially in the treated feedstock, resulting in a detrimental effect on the environment when directly applied as fertiliser on the soil. This study aims to predict the concentration of heavy metals in digestate through four different process parameters (Biogas yield - M1, Biodegradable fraction - M2, Dry matter - M3 and Power generation - M4) in full-scale biogas plants. For the validation of the process parameters, the predictions were compared against laboratory analyses of feedstocks and digestates samples from mono- and co-digestion processes. The convergence between the conversion factors based on laboratory data and process parameters (CLD and CFA, respectively) ranged in the following order: M3 > M2 > M1 > M4. Based on laboratory analyses, better predictions were obtained for Al, Cr, Cu, Fe, Mn, and Zn employing M3. Moreover, a robust convergence was achieved between the CLD and CFA conversion factors for the mono-digestion process. Further assessment of a diverse range of feedstocks is needed to increase the convergence between the conversion factors based on process parameters and laboratory data, specifically for the co-digestion process M3. The concentrations of Cd, Co, Ni, and Pb elements were below the detection limits, whereas Cr, Cu, and Zn did not exceed the legal threshold limits of the legislations.