ABSTRACT
This investigation was undertaken to study the associations of the polymorphic variants of the HLA-DRBI and HLA-DQB1 loci with the development of cystic echinococcosis in children. The material for the investigation was collected from 57 children admitted for surgery to the clinic of the Department of Pediatric Surgery, Orthopedics, and Anesthesiology, Bashkir State Medical University (Ufa). The PROTRANS kit (Germany) was used to isolate DNA samples from peripheral venous blood served as an object of the investigations. HLA specificities were typed by polymerase chain reaction. Molecular genetic studies established the association of DRB1*07, DQB1*0.9, DQB1*02 specificities with the increased risk of cystic echinococcosis in children. The echinococcosis cyst suppuration-complicated course of the disease was found to be more frequently encountered in DQB1*02 and DRB1*03 allele carriers.
Subject(s)
Echinococcosis/genetics , Echinococcus/immunology , Epitopes/genetics , HLA-DQ beta-Chains/genetics , HLA-DRB1 Chains/genetics , Alleles , Animals , Antigenic Variation/genetics , Antigenic Variation/immunology , Bashkiria , Case-Control Studies , Child , Child, Preschool , Cysts , Echinococcosis/blood , Echinococcosis/immunology , Epitopes/immunology , Female , Gene Frequency , Genetic Predisposition to Disease , HLA-DQ beta-Chains/blood , HLA-DQ beta-Chains/immunology , HLA-DRB1 Chains/blood , HLA-DRB1 Chains/immunology , Haplotypes , Heterozygote , Humans , Immunophenotyping , Male , Polymerase Chain Reaction , SuppurationABSTRACT
DNA samples isolated from peripheral venous blood lymphocytes in 73 children with hydatid disease were studied. The polymorphism of exon 7 (A4889G) of the CYP1A1 gene was analyzed by polymerase chain reaction, followed by hydrolysis with restriction endonuclease HincII. The material for E. granulosus genotypes to be studied was obtained from the germinal layer of larvocysts. The fragment of the mitochondrial gene encoding for the first subunit of cytochome-C-oxidase was as a DNA marker. The amplified E. granulosus DNA fragments underwent direct sequencing and a genotype was identified. The findings have led to the conclusion that carriage of polymorphic allele Val of exon 7 (A4889G) of the CYP1A1 gene in those infested with E. granulosus genotype G1 (common, sheep strain) is a risk factor of the development of the clinical form of echinococcosis granulosus.