Subject(s)
Colorectal Neoplasms/genetics , Interferon-gamma/genetics , Interleukin-1/genetics , Polymorphism, Genetic , Adult , Aged , Alleles , Case-Control Studies , China , Colorectal Neoplasms/pathology , Female , Genetic Association Studies , Genotype , Humans , Male , Middle Aged , Neoplasm Grading , Neoplasm Staging , Receptors, Interleukin-1/genetics , Sequence Analysis, DNAABSTRACT
OBJECTIVES: To investigate the microorganisms in expressed prostatic secretions (EPS) of both chronic prostatitis patients and normal young male adults and to determine which microorganisms are associated with the degree of intraprostatic inflammation. METHODS: Specific polymerase chain reaction (PCR) technology was performed to confirm Neisseria gonorrhoeae, Mycobacterium tuberculosis, Mycoplasma and Chlamydia trachomatis as well as human papilloma virus (HPV), herpes simplex virus type 2 (HSV-2) and cytomegalovirus (CMV). Universal primer PCR technology was carried out to detect 16S bacteria rDNA, followed by cloning and sequencing of the entire 16S rDNA product. RESULTS: The total number of bacteria and/or viruses detected by PCR assays was significantly associated with disease severity (p < 0.001). The white blood cell count and lecithin level was significantly correlated with the number of detected bacteria and/or viruses (p = 0.001 and p = 0.046, respectively). 17 bacterial isolates were identified from 14 EPS samples by 16S rDNA sequencing. CONCLUSIONS: Various microorganisms including Ureaplasma urealyticum, C. trachomatis, CMV, HPV and HSV-2 were identified in the EPS from patients with type III prostatitis. HPV infection may be associated with the degree of intraprostatic inflammation.
Subject(s)
Prostate/metabolism , Prostate/microbiology , Prostatitis/microbiology , Adolescent , Adult , Alphapapillomavirus/isolation & purification , Case-Control Studies , Chlamydia trachomatis/isolation & purification , Chronic Pain , Cytomegalovirus/isolation & purification , Herpesvirus 2, Human/isolation & purification , Humans , Male , Middle Aged , Mycobacterium tuberculosis/isolation & purification , Mycoplasma/isolation & purification , Neisseria gonorrhoeae/isolation & purification , Polymerase Chain Reaction , Prospective Studies , Sequence Analysis, DNA , Young AdultABSTRACT
AIM: To study whether macrophage migration inhibitory factor (MIF)-173 gene polymorphism correlates with inflammatory bowel disease (IBD) in Chinese Han population. METHODS: MIF-173 single nucleotide polymorphism (SNP) was genotyped by tetra-primer amplification refractory mutation system (ARMS) and restriction fragment length polymorphisms (RFLP)-PCR in 142 healthy subjects and 98 patients with inflammatory bowel disease (IBD). RESULTS: There were no discrepancies between the results obtained by tetra-primer ARMS and RFLP-PCR. The frequency of MIF-173 CC genotype was significantly higher in patients with ulcerative colitis (UC) 15.5% than in healthy individuals 5.6% (chi(2)=6.066, P=0.018, OR=3.067 and 95% CI=1.257-7.482). There was a trend towards a higher frequency of CC genotype among CD patients compared with healthy controls, however this did not attain the statistical significance (P=0.245). CONCLUSION: MIF-173 CC genotype may be associated with susceptibility to UC.
