Subject(s)
Cell Movement , Cell Proliferation , Lung Neoplasms , Neoplasm Invasiveness , Tripartite Motif Proteins , Ubiquitin-Protein Ligases , Humans , Lung Neoplasms/genetics , Lung Neoplasms/pathology , Lung Neoplasms/metabolism , Cell Proliferation/genetics , Cell Movement/genetics , Tripartite Motif Proteins/genetics , Tripartite Motif Proteins/metabolism , Ubiquitin-Protein Ligases/genetics , Ubiquitin-Protein Ligases/metabolism , Gene Knockdown Techniques , Cell Line, TumorABSTRACT
Lung cancer is the most commonly diagnosed type of cancer worldwide. Although TRIM65 is an important protein involved in white matter lesion, the role of TRIM65 in human cancer remains less understood. Here, we reported that TRIM65 was significantly overexpressed in lung cancer tissues compared with adjacent normal lung tissues. Furthermore, TRIM65 expression was closely related to overall survival of patients with lung cancer. Knock down of TRIM65 in two lung cancer cell lines, SPC-A-1 and NCI-H358, resulted in a significant reduction in cell proliferation, migration, invasion and adhesion and a dramatic increase in G0-G1 phase arrest and apoptosis. In vivo tumorigenesis experiment also revealed that depletion of TRIM65 expression inhibited NCI-H358 cell growth. Moreover, based on gene set enrichment analysis (GSEA) with The Cancer Genome Atlas (TCGA) dataset, we found that TRIM65 was positive related to cell cycle, metastasis up and RHOA-REG pathways, which was further validated by RT-PCR and Western blot in TRIM65 knockdown lung cancer cells and indicated a possible mechanism underlying its effects on lung cancer. In summary, our study suggests that TRIM65 may work as an oncogene and a new effective therapeutic target for lung cancer treatment.
Subject(s)
Cell Movement , Cell Proliferation , Gene Knockdown Techniques , Lung Neoplasms/genetics , Tripartite Motif Proteins/genetics , Ubiquitin-Protein Ligases/genetics , A549 Cells , Apoptosis , Cell Adhesion , Cell Cycle Checkpoints , Computational Biology , Databases, Genetic , Gene Expression Regulation, Neoplastic , Gene Regulatory Networks , Humans , Lung Neoplasms/metabolism , Lung Neoplasms/pathology , Lung Neoplasms/therapy , Neoplasm Invasiveness , RNA Interference , Signal Transduction , Time Factors , Transfection , Tripartite Motif Proteins/metabolism , Ubiquitin-Protein Ligases/metabolism , rhoA GTP-Binding Protein/genetics , rhoA GTP-Binding Protein/metabolismABSTRACT
A 3D porous aluminum(III) trisphosphonate, constructed from 1D inorganic aluminum phosphate chains and tripodal organic linkers, contains large hexagonal channels (1.24 nm in diameter) and a highly accessible void (50.3%) which allow it to have a fast and relatively high uptake of H2, N2 and CO2.
Subject(s)
Aluminum/chemistry , Coordination Complexes/chemistry , Organophosphorus Compounds/chemistry , Adsorption , Carbon Dioxide/chemistry , Hydrogen/chemistry , Nitrogen/chemistry , PorosityABSTRACT
Simultaneous determination of 9 (fluoro)quinolone antibiotics (FQs) was accomplished by capillary electrophoresis-ultraviolet (CE-UV) based on poly(methacrylic acid-co-ethylene glycol dimethacrylate) (MAA-EGDMA) monolith microextraction (PMME) coupled with on-line preconcentration technique of field-amplified sample stacking (FASS). The effects of composition of the acid and organic solvent in the sample solution, sampling time, and voltage on the efficiency of the sample stacking have been systematically investigated. Several parameters that influence extraction efficiency for PMME such as pH of sample solution, extraction volume, and wash and desorption conditions were optimized. In the proposed method, a substantial increase in sensitivity for all the FQs tested was achieved by the combination of PMME procedure with on-line preconcentration of FASS prior to CE analysis. Good linearities were obtained for the 9 tested FQs with the correlation coefficients (R) above 0.9954. The limits of detection (S/N=3) were found to be 2.4-34.0 ng g(-1) and the recoveries ranged from 81.2 to 100% with relative standard deviations less than 11.3%. The proposed PMME-FASS-CE method was applied to the determination of FQs residues in chicken samples.
