Chronic consumption of imbalance diets high in sucrose or fat induces abdominal obesity with different pattern of metabolic disturbances and lost in Langerhans cells population.

AIM: Obesity is a worldwide health issue, associated with development of type 2 Diabetes Mellitus. The aim of this study is to analyze the effect of consumption of two hypercaloric diets on metabolic disturbance and beta cells damage. MAIN METHODS: Male Wistar rats were subjected to twelve months consumption of three diets: a Control balanced diet (CTD, carbohydrates 58 %, proteins 29 %, lipids 13 %) and two hypercaloric diets, high in sucrose (HSD, carbohydrates 68 %, proteins 22 %, lipids 10 %) or high in fat (HFD, carbohydrates 31 %, proteins 14 %, lipids 55 %). Serum levels of glucose, triglycerides and free fatty acids were measured after zoometric parameters determination. Antioxidant enzymes activity and oxidative stress-marker were measured in pancreas tissue among histological analysis of Langerhans islets. KEY FINDINGS: Although diets were hypercaloric, the amount of food consumed by rats decreased, resulting in an equal caloric consumption. The HSD induced hypertriglyceridemia and hyperglycemia with higher levels in free fatty acids (FFA, lipotoxicity); whereas HFD did not increased neither the triglycerides nor FFA, nevertheless the loss of islets' cell was larger. Both diets induced obesity with hyperglycemia and significant reduction in Langerhans islets size. SIGNIFICANCE: Our results demonstrate that consumption of HSD induces more significant metabolic disturbances that HFD, although both generated pancreas damage; as well hypercaloric diet consumption is not indispensable to becoming obese; the chronic consumption of unbalanced diets (rich in carbohydrates or lipids) may lead to abdominal obesity with metabolic and functional disturbances, although the total amount of calories are similar.

Oxidative stress in animal models of obesity caused by hypercaloric diets: A systematic review.

Obesity is a global health difficulty characterized by an excessive accumulation of fat that increases body weight. Obesity has been studied in multiple animal models, of which those in which it is induced by diet stand out. Due to the increase in this condition, other mechanisms have been addressed that are triggered by states of overweight or obesity, such as the appearance of oxidative stress. These models aim to relate obesity caused by diet and how it influences the development of oxidative stress. In this study, a systematic review of the literature of 39 articles that studied obesity due to the consumption of hypercaloric diets and the appearance of oxidative stress in different animal models was carried out. This review identified the models with the most excellent use and the characteristics of the most appropriate diets to characterize states of oxidative stress due to obesity. In addition, the advantages and disadvantages of each model used are provided, as well as the techniques used for the assessment of obesity, and oxidative stress, providing the information in such a way that there is a general overview of the existing models of the parameters that allow to adequately establish both variables studied, providing information that allows the researcher to choose the appropriate model and factors according to the interest and objectives of the present research.