ABSTRACT
ABA-insensitive 5 (ABI5) belongs to the basic leucine zipper class of transcription factors and is named for being the fifth identified Arabidopsis mutant unresponsive to ABA. To understand the influence of ABI5 in its active state on downstream gene expression and plant growth and development, we overexpressed the full-length ABI5 (A.t.MX-4) and the active forms of ABI5 with deleted transcriptional repression domains (A.t.MX-1, A.t.MX-2, and A.t.MX-3). Compared with the wild type, A.t.MX-1, A.t.MX-2, and A.t.MX-3 exhibited an increase in rosette leaf number and size, earlier flowering, increased thousand-seed weight, and significantly enhanced drought resistance. Thirty-five upregulated/downregulated proteins in the A.t.MX-1 were identified by proteomic analysis, and these proteins were involved in ABA biosynthesis and degradation, abiotic stress, fatty acid synthesis, and energy metabolism. These proteins participate in the regulation of plant drought resistance, flowering timing, and seed size at the levels of transcription and post-translational modification.
ABSTRACT
There is a known genetic susceptibility to anthracycline-induced cardiac dysfunction in childhood cancer survivors, but this has not been adequately shown in adolescent and young adult (AYA) patients. Our aim was to determine if the previously identified variants associated with cardiac dysfunction in childhood cancer patients affect AYA cancer patients similarly. Forty-five variants were selected for analysis in 253 AYAs previously treated with anthracyclines. We identified four variants that were associated with cardiac dysfunction: SLC10A2:rs7319981 (p = 0.017), SLC22A17:rs4982753 (p = 0.019), HAS3:rs2232228 (p = 0.023), and RARG:rs2229774 (p = 0.050). HAS3:rs2232228 and SLC10A2:rs7319981 displayed significant effects in our AYA cancer survivor population that were in the opposite direction than that reported in childhood cancer survivors. Genetic variants in the host genes were further analyzed for additional associations with cardiotoxicity in AYA cancer survivors. The host genes were then evaluated in a panel of induced pluripotent stem cell-derived cardiomyocytes to assess changes in levels of expression when treated with doxorubicin. Significant upregulation of HAS3 and SLC22A17 expression was observed (p < 0.05), with non-significant anthracycline-responsivity observed for RARG. Our study demonstrates that there is a genetic influence on cardiac dysfunction in AYA cancer patients, but there may be a difference in the role of genetics between childhood and AYA cancer survivors.
Subject(s)
Anthracyclines , Cancer Survivors , Cardiotoxicity , Genetic Predisposition to Disease , Humans , Adolescent , Anthracyclines/adverse effects , Young Adult , Male , Female , Cardiotoxicity/genetics , Adult , Myocytes, Cardiac/drug effects , Myocytes, Cardiac/metabolism , Polymorphism, Single Nucleotide/genetics , Neoplasms/drug therapy , Neoplasms/genetics , Heart Diseases/chemically induced , Heart Diseases/genetics , Antibiotics, Antineoplastic/adverse effects , Risk FactorsABSTRACT
Subject to environmental influences such as temperature, humidity, light, and microorganisms, leather-based relics are easily be deteriorated, which will damage their historical value. In this work, gelatin/tannic acid composites (Gel/TA) was prepared and characterized to investigate their potential as a novel approach for reinforcing and protecting leather-based relics. According to the characterization, it was found that hydrogen bonds was formed between gelatin and TA, and Gel/TA presented increases in the antioxidant capacity(82.12 %), compared to the gelatin. After reinforcing the aged leather with Gel/TA, the cross-linking between leather collagen fibers is more closely interconnected. The mechanical properties and antibacterial activity of leather-based relics are significantly improved. The oxidation resistance of leather-based relics increased from 20.72 % to 63.03 %. Under the condition of accelerated simulated aging, the time to reduce the mechanical properties of reinforced leather by 50 % was extended from 7.6 days to 10 days. At the same time, the color of leather-based relics changed slightly. This result suggested that the Gel/TA composites play an important role in the filling and reinforcement protection of leather artifacts. This work will put forward a feasible way to producing sustainable multifunctional materials for leather-based relics.
Subject(s)
Gelatin , Tannins , Gelatin/chemistry , Tannins/chemistry , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/pharmacology , Antioxidants/chemistry , Animals , Mechanical Phenomena , PolyphenolsABSTRACT
Designing and developing high-performance shielding materials against electromagnetic interference is of utmost importance due to the rapid advancement of wireless telecommunication technologies. Such materials hold both fundamental and technological significance. A three-stage process is presented for creating ultralight, flexible aerogels from biomass to shield against electromagnetic interference. Collagen fibers sourced from leather solid waste are used for: (i) freeze-drying preparation of collagen fibers/poly(vinyl alcohol) (PVA) aerogels, (ii) adsorption of silver nanowires (AgNWs) onto collagen fiber/PVA aerogels, and (iii) Hydrophobic modification of collagen fiber/PVA/AgNWs aerogels with 1H, 1H, 2H, 2H-perfluorodecyltriethoxysilane (POTS). Scanning electron microscopy studies reveal that an interweaving of AgNWs and collagen fiber/PVA porous network has formed a conductive network, exhibiting an electrical conductivity of 103 S·m-1. The electromagnetic interference shielding effectiveness reached more than 62 dB, while the density was merely 5.8 mg/cm3. The collagen fiber/PVA/AgNWs/POTS aerogel displayed an even better electromagnetic shielding efficiency of 73 dB and water contact angle of 147°. The study results emphasize the distinctive capacity of leather solid waste to generate cost-effective, ecofriendly, and highly efficient electromagnetic interference shielding materials.
ABSTRACT
The development of functional textiles combining conventional apparel with advanced technologies for personal health management (PHM) has garnered widespread attention. However, the current PHM textiles often achieve multifunctionality by stacking functional modules, leading to poor durability and scalability. Herein, a scalable and robust PHM textile is designed by integrating electrical, radiative, and solar heating, electromagnetic interference (EMI) shielding, and piezoresistive sensing performance onto cotton fabric. This is achieved through an uncomplicated screen-printing process using silver paste. The conductivity of the PHM textile is ≈1.6â × 104 S m-1, ensuring an electric heating temperature of ≈134 °C with a low voltage of 1.7 V, as well as an EMI shielding effectiveness of ≈56 dB, and human motion monitoring performance. Surprisingly, the radiative/solar heating capability of the PHM textile surpasses that of traditional warm leather. Even after undergoing rigorous physical and chemical treatments, the PHM textile maintains terrific durability. Additionally, the PHM textile possesses maneuverable scalability and comfortable wearability. This innovative work opens up new avenues for the strategic design of PHM textiles and provides an advantageous guarantee of mass production.
ABSTRACT
Hydroxypropyl chitosan-Cs3Bi2Cl9 perovskite quantum dots (HPCS-PQDs) were synthesized by a simple ligand-assisted reprecipitation method via green hydroxypropyl chitosan as the ligand and used as the specific signal of a fluorescence probe to achieve the highly sensitive detection of hexavalent chromium (Cr(VI)) and compared with chitosan-Cs3Bi2Cl9 QDs (CS-PQDs). HPCS-PQDs with multiple active hydroxyl passivations were found to enhance the photoluminescence quantum yield (PLQY) by 90%. After being placed in aqueous solution and irradiated with ultraviolet light for 96 h the fluorescence intensity of HPCS-PQDs remained above 60%. The blue emission of HPCS-PQDs has a good selectivity and short response time (30 s) for Cr(VI). A good linear relationship is established between the fluorescence quenching rate of the HPCS-PQDs and concentration of Cr(VI) from 0.8 to 400 µM, with a limit of detection (LOD) of 0.27 µM. The fluorescence quenching mechanism is the static quenching and internal filtration effect caused by HPCS-PQDs forming a non-fluorescent ground-state complex with Cr(VI). The sensor can not only be used to detect Cr(VI) in water samples with high accuracy but can also be prepared as a test paper for the detection for Cr(VI).
ABSTRACT
BACKGROUND: Older adults have markedly increased risks of heart failure (HF), specifically HF with preserved ejection fraction (HFpEF). Identifying novel biomarkers can help in understanding HF pathogenesis and improve at-risk population identification. This study aimed to identify metabolites associated with incident HF, HFpEF, and HF with reduced ejection fraction and examine risk prediction in older adults. METHODS: Untargeted metabolomic profiling was performed in Black and White adults from the ARIC study (Atherosclerosis Risk in Communities) visit 5 (n=3719; mean age, 75 years). We applied Cox regressions to identify metabolites associated with incident HF and its subtypes. The metabolite risk score (MRS) was constructed and examined for associations with HF, echocardiographic measures, and HF risk prediction. Independent samples from visit 3 (n=1929; mean age, 58 years) were used for replication. RESULTS: Sixty metabolites (hazard ratios range, 0.79-1.49; false discovery rate, <0.05) were associated with incident HF after adjusting for clinical risk factors, eGFR, and NT-proBNP (N-terminal pro-B-type natriuretic peptide). Mannonate, a hydroxy acid, was replicated (hazard ratio, 1.36 [95% CI, 1.19-1.56]) with full adjustments. MRS was associated with an 80% increased risk of HF per SD increment, and the highest MRS quartile had 8.7× the risk of developing HFpEF than the lowest quartile. High MRS was also associated with unfavorable values of cardiac structure and function. Adding MRS over clinical risk factors and NT-proBNP improved 5-year HF risk prediction C statistics from 0.817 to 0.850 (∆C, 0.033 [95% CI, 0.017-0.047]). The association between MRS and incident HF was replicated after accounting for clinical risk factors (P<0.05). CONCLUSIONS: Novel metabolites associated with HF risk were identified, elucidating disease pathways, specifically HFpEF. An MRS was associated with HF risk and improved 5-year risk prediction in older adults, which may assist at at-risk population identification.
Subject(s)
Heart Failure , Humans , Aged , Middle Aged , Heart Failure/diagnosis , Heart Failure/epidemiology , Heart Failure/etiology , Stroke Volume , Prospective Studies , Biomarkers , Risk Factors , Peptide Fragments , Natriuretic Peptide, Brain , PrognosisABSTRACT
Casein-based hydrogels (Casein Gels) possess advantageous properties, including mechanical strength, stability, biocompatibility, and even adhesion, conductivity, sensing capabilities, as well as controlled-releasing behavior of drugs. These features are attributed to their gelation methods and functionalization with various polymers. Casein Gels is an important protein-based material in the food industry, in terms of dairy and functional foods, biological and medicine, in terms of carrier for bioactive and sensitive drugs, wound healing, and flexible sensors and wearable devices. Herein, this review aims to highlight the importance of the features mentioned above via a comprehensive investigation of Casein Gels through multiple directions and dimensional applications. Firstly, the composition, structure, and properties of casein, along with the gelation methods employed to create Casein Gels are elaborated, which serves as a foundation for further exploration. Then, the application progresses of Casein Gels in dairy products, functional foods, medicine, flexible sensors and wearable devices, are thoroughly discussed to provide insights into the diverse fields where Casein Gels have shown promise and utility. Lastly, the existing challenges and future research trends are highlighted from an interdisciplinary perspective. We present the latest research advances of Casein Gels and provide references for the development of multifunctional biomass-based hydrogels.
Subject(s)
Caseins , Hydrogels , Biomass , Electric Conductivity , Functional FoodABSTRACT
Heart failure (HF) causes substantial morbidity and mortality but its pathobiology is incompletely understood. The proteome is a promising intermediate phenotype for discovery of novel mechanisms. We measured 4877 plasma proteins in 13,900 HF-free individuals across three analysis sets with diverse age, geography, and HF ascertainment to identify circulating proteins and protein networks associated with HF development. Parallel analyses in Atherosclerosis Risk in Communities study participants in mid-life and late-life and in Trøndelag Health Study participants identified 37 proteins consistently associated with incident HF independent of traditional risk factors. Mendelian randomization supported causal effects of 10 on HF, HF risk factors, or left ventricular size and function, including matricellular (e.g. SPON1, MFAP4), senescence-associated (FSTL3, IGFBP7), and inflammatory (SVEP1, CCL15, ITIH3) proteins. Protein co-regulation network analyses identified 5 modules associated with HF risk, two of which were influenced by genetic variants that implicated trans hotspots within the VTN and CFH genes.
Subject(s)
Atherosclerosis , Heart Failure , Humans , Proteomics , Risk Factors , Phenotype , Carrier Proteins/genetics , Glycoproteins/genetics , Extracellular Matrix Proteins/geneticsABSTRACT
Hepatic venous pressure gradient (HVPG) is the gold standard for evaluating clinically significant portal hypertension (CSPH). However, reliable noninvasive methods are limited. Our study aims to investigate the diagnostic value of serum Golgi protein 73 (GP73) for CSPH in patients with compensated cirrhosis. The study enrolled 262 consecutive patients with compensated cirrhosis from three centers in China from February 2021 to September 2023, who underwent both serum GP73 tests and HVPG measurements. CSPH was defined as HVPG ≥ 10 mmHg. Diagnostic accuracy was evaluated using the areas under the receiver operating characteristic curve (AUC). The prevalence of CSPH was 56.9% (n = 149). There were significant differences between the CSPH and non-CSPH groups in the median serum GP73 level (126.8 vs. 73.1 ng/mL, p < 0.001). GP73 level showed a significant positive linear correlation with HVPG (r = 0.459, p < 0.001). The AUC for the diagnosis of CSPH using serum GP73 alone was 0.75 (95% confidence interval [CI] 0.68-0.81). Multivariate logistic regression analysis determined that the levels of GP73, platelets and international normalized ratio were independently associated with CSPH. The combination of these three markers was termed "IP73" score with an AUC value of 0.85 (95% CI 0.80-0.89) for CSPH. Using 0 as a cut-off value, the specificity and sensitivity of IP73 score were 77.9% and 81.9%, respectively. The IP73 score offers a novel, simple and noninvasive method of assessing CSPH in patients with compensated cirrhosis. A cut-off value of the IP73 score at 0 can distinguish patients with or without CSPH.
Subject(s)
Elasticity Imaging Techniques , Hypertension, Portal , Humans , Biomarkers , Hypertension, Portal/complications , Hypertension, Portal/diagnosis , Liver , Liver Cirrhosis/complications , Liver Cirrhosis/diagnosis , ROC Curve , Time FactorsABSTRACT
Passive heating textiles (PHTs) have drawn increasing attention due to the advantages of energy-conservation heating. However, the heating capabilities of current PHTs are typically static and non-tunable, presenting poor adaptation to dynamic winter. Herein, a novel Janus textile with tunable heating modes is developed by constructing a customized structure with asymmetric optical properties. This Janus textile is created by coating one side of a cotton fabric with silver nanowires (AgNWs) and then applying transition metal carbides/nitrides (MXene) to the other side. The MXene side exhibits high solar absorptivity and low mid-infrared emissivity, while the AgNWs side has moderate solar absorptivity and mid-infrared emissivity. This structure ensures that the solar and radiative heating temperatures of the MXene side are 16 °C and 1.7 °C higher than those of the AgNWs side. This distinction allows for on-demand, accurate adjustments in solar and radiative heating capabilities by flipping the textile according to ambient temperature. Furthermore, this innovative design also features desired electric heating, thermal camouflage, self-cleaning and antibacterial properties, electromagnetic interference shielding, durability, and wearability. The Janus textile enables precise thermoregulation of the human body to adapt to variable cold weather, making it essential for optimal personal thermal management and climate change mitigation.
ABSTRACT
Highly robust flexible multifunctional film with excellent electromagnetic interference shielding and electrothermal/photothermal characteristics are highly desirable for aerospace, military, and wearable devices. Herein, an asymmetric gradient multilayer structured bacterial cellulose@Fe3O4/carbon nanotube/Ti3C2Tx (BC@Fe3O4/CNT/Ti3C2Tx) multifunctional composite film is fabricated with simultaneously demonstrating fast Joule response, excellent EMI shielding effectiveness (EMI SE) and photothermal conversion properties. The asymmetric gradient 6-layer composite film with 40% of Ti3C2Tx possesses excellent mechanical performance with exceptional tensile strength (76.1 MPa), large strain (14.7%), and good flexibility. This is attributed to the asymmetric gradient multilayer structure designed based on the hydrogen bonding self-assembly strategy between Ti3C2Tx and BC. It achieved an EMI SE of up to 71.3 dB, which is attributed to the gradient "absorption-reflection-reabsorption" mechanism. Furthermore, this composite film also exhibits excellent low-voltage-driven Joule heating (up to 80.3 °C at 2.5 V within 15 s) and fast-response photothermal performance (up to 101.5 °C at 1.0 W cm-2 within 10 s), which is attributed to the synergistic effect of heterostructure. This work demonstrates the fabrication of multifunctional bacterial cellulose@Fe3O4/carbon nanotube/Ti3C2Tx composite film has promising potentials for next-generation wearable electronic devices in energy conversion, aerospace, and artificial intelligence.
Subject(s)
Cellulose , Nanotubes, Carbon , Wearable Electronic Devices , Cellulose/chemistry , Nanotubes, Carbon/chemistry , Titanium/chemistry , Bacteria , Nanocomposites/chemistryABSTRACT
The enzyme nitric oxide synthase 2 or inducible NOS (NOS2), reactive oxygen species (ROS) and nitric oxide (NO) are important participants in various inflammatory and immune responses. However, the functional significances of the correlations among piscine NOS2, ROS and NO during pathogen infection remain unclear. In teleost, there are two nos2 genes (nos2a and nos2b). It has been previously reported that zebrafish nos2a behaves as a classical inducible NOS, and nos2b exerts some functions similar to mammalian NOS3. In the present study, we reported the functional characterization of zebrafish nos2a during bacterial infection. We found that zebrafish nos2a promoted bacterial proliferation, accompanied by an increased susceptibility to Edwardsiella piscicida infection. The nagative regulation of zebrafish nos2a during E. piscicida infection was characterized by the impaired ROS levels, the induced NO production and the decreased expressions of proinflammatory cytokines, antibacterial genes and oxidant factors. Furthermore, although both inducing ROS and inhibiting NO production significantly inhibited bacterial proliferation, only inhibiting NO production but not inducing ROS significantly increased resistance to E. piscicida infection. More importantly, ROS supplementation and inhibition of NO completely abolished this detrimental consequence mediated by zebrafish nos2a during E. piscicida infection. All together, these results firstly demonstrate that the innate response mediated by zebrafish nos2a in promoting bacterial proliferation is dependent on the lower ROS level and higher NO production. The present study also reveals that inhibition of NO can be effective in the protection against E. piscicida infection.
Subject(s)
Edwardsiella , Enterobacteriaceae Infections , Animals , Cytokines , Zebrafish , Nitric Oxide/metabolism , Reactive Oxygen Species/metabolism , Nitric Oxide Synthase Type II/genetics , Nitric Oxide Synthase Type II/metabolism , Cell Proliferation , Edwardsiella/physiology , Mammals/metabolismABSTRACT
Self-propelled separation materials, that is, motor, are one of the keys to realizing smart oil-water separation. Although three-dimensional sponges such as commercial melamine sponge (MS) exhibit excellent oil-water separation ability, they cannot move by themselves on water. Aiming at solving this problem, a polydimethylsiloxane (PDMS) and molybdenum disulfide (MoS2) modified MS motor (PDMS@MS/MoS2) with an asymmetric multilayer structure was prepared, in which the photothermal layer MoS2 provided the propelling force for the motor under infrared light irradiation, and the middle layer PDMS was used as the superhydrophobic modified agent and adhesive agent between commercial MS and MoS2 powder. PDMS coated MS (PDMS@MS) as the superhydrophobic layer showed good superhydrophobic ability (153.1°) and oil-water separation capacity (52.33 g/g to liquid paraffin). Furthermore, the introduction of MoS2 made the speed of the sponge motor reach 8.27 mm s-1 with a removal quantity of 12.20 g/g for cyclohexane. After recycling 8 times, the contact angle, cyclohexane capturing amount, and average velocity of the motor were 150.3°, 11.40 g/g, and 8.41 mm/s, respectively. Meanwhile, PDMS@MS/MoS2 kept a similar light-propelling velocity (â¼8 mm) at different pH values and in simulated seawater, demonstrating that the light-propelling motor possessed a good cycle and practical performance, which provides a possibility for the directional light propulsion of a sponge motor in oil-water separation.
ABSTRACT
The non-degradable traditional polyethylene (PE) mulch film has caused great harm to both the ecological environment as well as human health. Therefore, the biodegradable bone gelatin (B-Gel) was innovatively selected to build the mulch film. To further enhance the toughness of the B-Gel mulch films, a POSS star-shaped polymer/bone gelatin (P(POSS-AGE-HEA)/B-Gel) composite was prepared by introducing POSS star-shaped polymer into B-Gel via in situ polymerization using polyhedral oligomeric silsesquioxane (POSS), allyl glycidyl ether (AGE) and hydroxyethyl acrylate (HEA) as raw material, and then was cast to obtain the P(POSS-AGE-HEA)/B-Gel mulch film. The epoxy group of POSS star-shaped polymer with the -COOH and -NH2 of B-Gel forms a covalent bond, and the hydroxyl group with the active groups of B-Gel forms hydrogen bonds. Meanwhile, the multiple side chains of POSS star-shaped polymer are intertwined with B-Gel. These covalent and hydrogen bonds as sacrificial bonds for effective energy dissipation giving the bone gelatin-based film excellent mechanical properties with a tensile strength of 7.56 ± 0.64 MPa and elongation at break of 197.49 ± 17.63 %. Additionally, it also demonstrated sound water vapor barrier, surface hydrophobicity, light transmittance and the effect of facilitating the growth and germination ratio (93.75 %) of wheat.
Subject(s)
Gelatin , Polymers , Humans , Polymers/chemistry , Tensile StrengthABSTRACT
Heavy ion beam (HIB) is an effective physical mutagen that has been widely used in plant mutational breeding. Systemic knowledge of the effects caused by different HIB doses at developmental and genomic levels will facilitate efficient breeding for crops. Here we examined the effects of HIB systematically. Kitaake rice seeds were irradiated by ten doses of carbon ion beams (CIB, 25 - 300 Gy), which is the most widely used HIB. We initially examined the growth, development and photosynthetic parameters of the M1 population and found that doses exceeding 125 Gy caused significant physiological damages to rice. Subsequently, we analyzed the genomic variations in 179 M2 individuals from six treatments (25 - 150 Gy) via whole-genome sequencing (WGS). The mutation rate peaks at 100 Gy (2.66×10-7/bp). Importantly, we found that mutations shared among different panicles of the same M1 individual are at low ratios, validating the hypothesis that different panicles may be derived from different progenitor cells. Furthermore, we isolated 129 mutants with distinct phenotypic variations, including changes in agronomic traits, from 11,720 M2 plants, accounting for a 1.1% mutation rate. Among them, about 50% possess stable inheritance in M3. WGS data of 11 stable M4 mutants, including three lines with higher yields, reveal their genomic mutational profiles and candidate genes. Our results demonstrate that HIB is an effective tool that facilitates breeding, that the optimal dose range for rice is 67 - 90% median lethal dose (LD50), and that the mutants isolated here can be further used for functional genomic research, genetic analysis, and breeding.
ABSTRACT
Flexible hydrogels have emerged as highly-desirable materials for wearable strain sensors. However, pristine biomass hydrogel systems are limited by their lack of stretchability, self-adhesion, and sensitivity. Here, a novel CA/MWCNT/PAAm double-network conductive hydrogel was developed through integrating casein (CA) micelles and multi-walled carbon nanotubes (MWCNT) into the polyacrylamide (PAAm) network. The resulting hydrogel displayed desired properties such as adhesiveness, toughness, self-healing, and near-infrared photothermal response. In this hybrid system, MWCNT were uniformly dispersed in the presence of casein micelles through hydrogen bonding and electrostatic interactions, favoring its role of nano reinforcement. Moreover, based on the "casein micelle-nanoparticle double cross-linking" mechanism and its double network structure, the prepared hydrogel showed high extensibility (2288 % ± 63 %), fast responsiveness (273 ± 5.13 ms), high sensitivity (GF = 12.46 ± 0.35), and a wide strain range (1-1000 %). Through consistent and repeated electrical inputs, this hydrogel was able to detect including large and small human movements, such as hand, leg, and swallowing motions. The results from this study provide a new way to fabricate bio-based hydrogel sensors with excellent mechanical and electrical properties.
Subject(s)
Nanotubes, Carbon , Wearable Electronic Devices , Humans , Caseins , Micelles , Adhesives/chemistry , Electric Conductivity , Hydrogels/chemistryABSTRACT
The vertical profiles of nitrogen dioxide (NO2) and formaldehyde (HCHO) in the troposphere at the Longfengshan (LFS) regional atmospheric background station (127°36' E, 44°44' N, 330.5 m above sea level) from 24 October 2020 to 13 October 2021 were retrieved from solar scattering spectra by multi-axis differential optical absorption spectroscopy (MAX-DOAS). We analyzed the temporal variations of NO2 and HCHO as well as the sensitivity of ozone (O3) production to the concentration ratio of HCHO to NO2. The largest NO2 volume mixing ratios (VMRs) occur in the near-surface layer for each month, with high values concentrated in the morning and evening. HCHO has an elevated layer around the altitude of 1.4 km consistently. The means ± standard deviations of vertical column densities (VCDs) and near-surface VMRs were 4.69 ± 3.72 ×1015 molecule·cm-2 and 1.22 ± 1.09 ppb for NO2, and they were 1.19 ± 8.35 × 1016 molecule·cm-2 and 2.41 ± 3.26 ppb for HCHO. The VCDs and near-surface VMRs for NO2 were high in the cold months and low in the warm months, while HCHO presented the opposite. The larger near-surface NO2 VMRs appeared in the condition associated with lower temperature and higher humidity, but this relationship was not found between HCHO and temperature. We also found the O3 production at the Longfengshan station was mainly in the NOx-limited regime. This is the first study presenting the vertical distributions of NO2 and HCHO in the regional background atmosphere of northeastern China, which are significant to enhancing the understanding of background atmospheric chemistry and regional ozone pollution processes.
ABSTRACT
The R-spondin protein family comprises four members (RSPO1-4), which are agonists of the canonical Wnt/ß-catenin pathway. Emerging evidence revealed that RSPOs should not only be viewed as agonists of the Wnt/ß-catenin pathway but also as regulators for tumor development and progression. Aberrant expression of RSPOs is related to tumorigenesis and tumor development in multiple cancers and their expression of RSPOs has also been correlated with anticancer immune cell signatures. More importantly, the role of RSPOs as potential target therapies and their implication in cancer progressions has been studied in the preclinical and clinical settings. These findings highlight the possible therapeutic value of RSPOs in cancer medicine. However, the expression pattern, effects, and mechanisms of RSPO proteins in cancer remain elusive. Investigating the many roles of RSPOs is likely to expand and improve our understanding of the oncogenic mechanisms mediated by RSPOs. Here, we reviewed the recent advances in the functions and underlying molecular mechanisms of RSPOs in tumor development, cancer microenvironment regulation, and immunity, and discussed the therapeutic potential of targeting RSPOs for cancer treatment. In addition, we also explored the biological feature and clinical relevance of RSPOs in cancer mutagenesis, transcriptional regulation, and immune correlation by bioinformatics analysis.KEY MESSAGESAberrant expressions of RSPOs are detected in various human malignancies and are always correlated with oncogenesis.Although extensive studies of RSPOs have been conducted, their precise molecular mechanism remains poorly understood.Bioinformatic analysis revealed that RSPOs may play a part in the development of the immune composition of the tumor microenvironment.