ABSTRACT
Antibody detection is the basis of large-scale sheep brucellosis diagnosis because of its sensitivity and specificity. In contrast, information on the cellular mediated immune (CMI) response triggered after Brucella melitensis infection, a cornerstone in the protection against this pathogen, is more limited, particularly regarding the effect of the virulence of the infecting strain in the induced CMI reaction. Here, the interferon-gamma (IFN-γ) profiles evoked after exposure by different routes to virulent (H38) and attenuated (Rev.1) B. melitensis strains in 14 pregnant sheep and 87 ewe lambs, respectively, were characterized accounting for different host-related factors, and compared with their serological response and with the basal IFN-γ responses observed in 155 animals non exposed to Brucella. No significant differences in the IFN-γ response of Rev.1 vaccinated animals depending on the inoculation route was observed, in contrast with their serological results. Response in H38-challenged followed a similar trend although peaked later, and an effect of the abortion on the IFN-γ response was detected. This information could help to understand the interaction bacteria-host that leads to its intracellular survival and could be useful for the design of new diagnostic approaches.
Subject(s)
Brucella melitensis/pathogenicity , Brucellosis/veterinary , Interferon-gamma/metabolism , Sheep Diseases/immunology , Animals , Brucella melitensis/immunology , Brucellosis/immunology , Brucellosis/microbiology , Case-Control Studies , Female , Gene Expression Regulation/immunology , Interferon-gamma/blood , Interferon-gamma/genetics , Sheep , Sheep Diseases/microbiology , VirulenceABSTRACT
La esclerodermia es una enfermedad sistémica-crónica que afecta la piel y órganos como el esófago, en un 80%; se inicia en el tercer o cuarto decenio de vida y es dos veces más frecuente en mujeres. El diagnóstico de esclerodermia esofágica se establece mediante estudios manométricos por la presencia de peristalsis normal en la parte proximal del esófago estriada, con ausencia de la misma en la parte distal de músculo liso.En el esófago se manifiesta una esofagitis péptica crónica que raramente produce complicaciones letales como el sangrado masivo ocurrido en el presente caso clínico.
The scleroderma is a systemic chronic disease that affects skin and the rest of the organs like the esophagus in 80% of the cases. It usually affects woman in the third or fourth decade of life. The diagnosis of scleroderma in the esophagus can be determined by performing and esohageal manometry to study the muscular contraction of proximal part of the esophagus and its abscense of distal part.A chronic peptic esophagitis is the clinical manifestation of scleroderma. It rarely produces lethal complications like massive hemorrhage which occurred in this clinical case.
