1.
Biochem Soc Trans
; 24(1): 86S, 1996 Feb.
Article
in English
| MEDLINE
| ID: mdl-8674768
2.
Br J Biomed Sci
; 52(3): 230-1, 1995 Sep.
Article
in English
| MEDLINE
| ID: mdl-8528001
3.
Biochem Biophys Res Commun
; 207(1): 432-7, 1995 Feb 06.
Article
in English
| MEDLINE
| ID: mdl-7857300
ABSTRACT
Ribonuclease B has become a paradigm as a simple example of an N-linked glycoprotein. We have found that certain affinity-purified preparations of this enzyme demonstrated a pronounced tendency to lose activity if stored as dilute aqueous solutions. Such inactivation is accelerated by the presence of NaCl, but can be counteracted by inclusion of high (1 mol/l) concentrations of xylose. Enzyme activity cannot be restored by addition of xylose after storage of the enzyme. In marked contrast to alpha-methyl-mannoside, xylose does not prevent ribonuclease B from binding to concanavalin A and so may be used to stabilize the enzyme during purification by lectin affinity chromatography.
Subject(s)
Ribonucleases/chemistry , Ribonucleases/metabolism , Xylose/pharmacology , Chromatography, Affinity , Enzyme Stability , Kinetics , Methylmannosides/pharmacology , Ribonucleases/isolation & purification , Solutions , Spectrophotometry, Ultraviolet
4.
Biochem Soc Trans
; 20(1): 71S, 1992 Feb.
Article
in English
| MEDLINE
| ID: mdl-1633993
Subject(s)
Concanavalin A/metabolism , Glycoside Hydrolases/analysis , Hexosaminidases/analysis , Ribonucleases/metabolism , Chromatography, Affinity , Glycoside Hydrolases/metabolism , Hexosaminidases/metabolism , Kinetics , Molecular Weight , Protein Binding , Ribonucleases/isolation & purification
5.
Biochem Soc Trans
; 20(1): 72S, 1992 Feb.
Article
in English
| MEDLINE
| ID: mdl-1633994
6.
Biochem Soc Trans
; 20(1): 73S, 1992 Feb.
Article
in English
| MEDLINE
| ID: mdl-1633995