ABSTRACT
Translationally controlled tumour protein (TCTP) may play an important role in the establishment or maintenance of parasitemia in a malarial infection. In this study, the potential of TCTP as a malaria vaccine was investigated in two trials. In the initial vaccine trial, Plasmodium falciparum TCTP (PfTCTP) was expressed in Saccharomyces cerevisiae and used to immunize BALB/c mice. Following challenge with Plasmodium yoelii YM, parasitemia was significantly reduced during the early stages of infection. In the second vaccine trial, the TCTP from P. yoelii and P. berghei was expressed in Escherichia coli and used in several mouse malaria models. A significant reduction in parasitemia in the early stages of infection was observed in BALB/c mice challenged with P. yoelii YM. A significantly reduced parasitemia at each day leading up to a delayed and reduced peak parasitemia was also observed in BALB/c mice challenged with the nonlethal Plasmodium chabaudi (P.c.) chabaudi AS. These results suggest that TCTP has an important role for parasite establishment and may be important for pathogenesis.
Subject(s)
Antibodies, Protozoan/blood , Malaria Vaccines/immunology , Malaria/prevention & control , Protozoan Proteins/immunology , Protozoan Proteins/physiology , Animals , Biomarkers, Tumor/chemistry , Biomarkers, Tumor/immunology , Female , Malaria/immunology , Malaria/parasitology , Malaria Vaccines/chemistry , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Parasitemia/immunology , Parasitemia/prevention & control , Plasmodium/immunology , Plasmodium chabaudi/immunology , Plasmodium chabaudi/physiology , Plasmodium falciparum/immunology , Plasmodium yoelii/immunology , Plasmodium yoelii/physiology , Protozoan Proteins/chemistry , Tumor Protein, Translationally-Controlled 1 , Vaccination , Vaccines, Synthetic/chemistry , Vaccines, Synthetic/immunologyABSTRACT
The statins, simvastatin and atorvastatin are the most widely prescribed drugs. Statins lower cholesterol levels through their action on HMG-CoA (3-hydroxy-3-methylglutaryl-CoA) reductase, an essential enzyme for the biosynthesis of cholesterol. Fungal HMG-CoA reductases are also inhibited by statins, resulting in reduced levels of ergosterol (the fungal equivalent of cholesterol) and concomitant growth inhibition. This effect occurs in a range of fungal species and possibly affects fungal colonization of people on statin therapy. Furthermore, it may suggest that statins could have a role in new antifungal therapies. Possibly associated with the reduction in ergosterol levels, statins also inhibit respiratory growth. In the yeast, Candida glabrata, passage with statins dramatically increased the frequencies of petite mutants that were devoid of mitochondrial DNA, suggesting that statins caused a defect in the maintenance of mitochondrial DNA. These observations in C. glabrata may provide further insights into side effects of statins in humans undergoing treatment for hypercholesterolaemia. In addition, C. glabrata may be highly useful for the preliminary screening of agents to reduce statin side effects.
Subject(s)
Antifungal Agents/pharmacology , Candida glabrata/classification , Candida glabrata/drug effects , DNA, Mitochondrial/drug effects , Heptanoic Acids/pharmacology , Pyrroles/pharmacology , Simvastatin/pharmacology , Atorvastatin , Candida glabrata/growth & development , DNA, Mitochondrial/physiology , Humans , Hydroxymethylglutaryl CoA Reductases/drug effectsABSTRACT
Saccharomyces cerevisiae mutants deficient in folate synthesis have been constructed and employed to study the utilisation of exogenous folates in yeast. One mutant specifically lacked dihydropteroate synthase while the second lacked dihydrofolate synthase. Exogenous folinic acid restored optimal growth to both strains. Folic acid did not generally rescue growth but spontaneous isolates capable of utilising folic acid were selected. The folic acid synthesis pathway in the folate utilising isolates was restored via transformation with FOL1 or FOL3 expression plasmids and transformants were tested for resistance to sulfamethoxazole (SMX). The presence of elevated levels of folic acid led to greatly reduced SMX sensitivity regardless of whether strains were folate utilisers or not.
Subject(s)
Anti-Infective Agents/pharmacology , Drug Resistance, Fungal , Folic Acid/metabolism , Saccharomyces cerevisiae/drug effects , Sulfamethoxazole/pharmacology , Microbial Sensitivity Tests , Mutation , Saccharomyces cerevisiae/enzymology , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/growth & developmentABSTRACT
Sulfa drugs have been used as antimicrobials for decades but resistance is now a problem. For major eukaryotic pathogens, including Plasmodium and Pneumocystis, sulfa drug testing is difficult or impossible. We have shown that the eukaryote yeast can be used as a model for the study of sulfa drugs within certain parameters. Fifteen sulfa drugs inhibited yeast growth in a manner indicating competition with p-aminobenzoate (pABA). Such competition resulted from direct addition of pABA or through increased expression of the pABA synthase gene (ABZ1). The model system predicts that overexpression of the pABA synthase gene can lead to drug resistance.
Subject(s)
4-Aminobenzoic Acid/pharmacology , Anti-Infective Agents/pharmacology , Saccharomyces cerevisiae/drug effects , Sulfonamides/pharmacology , Sulfones/pharmacology , Cloning, Molecular , Genetic Complementation Test , Microbial Sensitivity Tests , Saccharomyces cerevisiae/enzymology , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/growth & development , Transaminases/genetics , Transaminases/metabolismABSTRACT
HIV-1 Vpr is a virion-associated protein that can cause growth arrest when produced inside the cell but when added externally it can cause cell death. Employing the yeast model system, the C-terminal domain, in particular the sequence HFRIGCRHSRIG (Vpr(71-82)), is essential for both the growth arrest and cytocidal activities. Conservation of this sequence in HIV-2 and SIV suggests that these residues may be functionally important. Using site-directed mutagenesis we show that the most highly conserved aa residues, His71 and Gly75, were important for the cell cycle inhibitory effects. In contrast, we show that the wild-type Vpr(71-82) peptide and three variants of this peptide with Gly75 changed to Ser, Ala, and Ile all exhibited the same cytocidal activity suggesting that the intracellular and extracellular effects are unrelated.
Subject(s)
Cell Division , Gene Products, vpr/physiology , HIV-1/physiology , Amino Acid Sequence , Amino Acid Substitution , Base Sequence , Cell Death , Cloning, Molecular , DNA, Viral/analysis , Escherichia coli , Gene Products, vpr/chemistry , Gene Products, vpr/genetics , Glycine/physiology , HIV-1/pathogenicity , Histidine/physiology , Models, Biological , Molecular Sequence Data , Mutagenesis, Site-Directed , Protein Conformation , Saccharomyces cerevisiae , Sequence Homology, Amino Acid , vpr Gene Products, Human Immunodeficiency VirusABSTRACT
We have found that the hemolytic and cytotoxic activities of myristoylated Nef N-terminal peptides require a net positive charge in the first seven amino residues of the sequence. The activities are considerably less dependent on the secondary structure of the peptides. Film balance studies showed that both active and inactive peptides interacted with neutral phospholipid monolayers, suggesting that binding to neutral lipids was not a sufficient condition for lytic activity. It was also found that nonmyristoylated N-terminal peptide did not interact to the same extent with the monolayer, indicating that myristoylation was essential for lipid interaction. It is considered that the positively charged residues of the proximate N terminus of Nef interact with acidic lipids of biological membranes, reinforcing the weak membrane-targeting properties of the myristyl chain. Parallels are drawn between this mode of interaction with membranes and that of members of the Src family of proteins, which are also myristoylated and have positively charged residues in their proximate N termini. In particular, these proteins and Nef also have serine residues in their proximal N-terminal regions, which when phosphorylated could neutralize the positive charge and thus provide a mechanism for modulating membrane interaction.
Subject(s)
Gene Products, nef/chemistry , Gene Products, nef/physiology , HIV-1/physiology , Protein Structure, Secondary , Amino Acid Sequence , Animals , Hemolysis , Humans , Membrane Fusion , Molecular Sequence Data , Myristic Acid , Peptides/chemistry , Peptides/physiology , Sheep , Structure-Activity Relationship , nef Gene Products, Human Immunodeficiency VirusABSTRACT
Vpr, one of the accessory gene products encoded by HIV-1, is a 96-residue protein with a number of functions, including targeting of the viral pre-integration complex to the nucleus and inducing growth arrest of dividing cells. We have characterized by 2D NMR the solution conformations of bioactive synthetic peptide fragments of Vpr encompassing a pair of H(F/S)RIG sequence motifs (residues 71-75 and 78-82 of HIV-1 Vpr) that cause cell membrane permeabilization and death in yeast and mammalian cells. Due to limited solubility of the peptides in water, their structures were studied in aqueous trifluoroethanol. Peptide Vpr59-86 (residues 59-86 of Vpr) formed an alpha-helix encompassing residues 60-77, with a kink in the vicinity of residue 62. The first of the repeated sequence motifs (HFRIG) participated in the well-defined alpha-helical domain whereas the second (HSRIG) lay outside the helical domain and formed a reverse turn followed by a less ordered region. On the other hand, peptides Vpr71-82 and Vpr71-96, in which the sequence motifs were located at the N-terminus, were largely unstructured under similar conditions, as judged by their C(alpha)H chemical shifts. Thus, the HFRIG and HSRIG motifs adopt alpha-helical and turn structures, respectively, when preceded by a helical structure, but are largely unstructured in isolation. The implications of these findings for interpretation of the structure-function relationships of synthetic peptides containing these motifs are discussed.
Subject(s)
Gene Products, vpr/chemistry , Peptide Fragments/chemistry , Amino Acid Sequence , Cell Membrane Permeability , Magnetic Resonance Spectroscopy , Models, Molecular , Molecular Sequence Data , Protein ConformationABSTRACT
The human immunodeficiency virus type 1 (HIV-1) Nef protein is essential for AIDS pathogenesis, but its function remains highly controversial. During stresses such as growth in the presence of copper or at elevated temperature, myristylated Nef is released from yeast cells and, after extended culture in stationary phase, it accumulates in the supernatant as a dense membranous material that can be centrifuged into a discrete layer above the cell pellet. This material is unique to Nef-producing cells and represents a convenient source of Nef that may have application in further biological studies. Within the yeast cell, electron microscopic examination shows that Nef localises in novel, membrane-bound bodies. These data support the evidence for a role of Nef in membrane perturbation and suggest that there may be a similar localisation for myristylated Nef in HIV-1 infected cells.
Subject(s)
Cell Membrane/metabolism , Gene Products, nef/metabolism , Myristic Acids/metabolism , Cell Membrane/ultrastructure , Cell Nucleus , Centrifugation , Chromatography, Gel , Gene Products, nef/genetics , Inclusion Bodies/ultrastructure , Microscopy, Immunoelectron , Recombinant Proteins/metabolism , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/metabolism , Saccharomyces cerevisiae/ultrastructure , TransfectionABSTRACT
Myristoylated 21- and 25-residue N-terminal peptides of the Nef protein of HIV-1 lysed human erythrocytes and were cytotoxic toward a human CD4+ T cell line, CEM, and primary human peripheral blood mononuclear cells (PBMCs). The corresponding nonmyristoylated N-terminal peptides were only very weakly hemolytic and cytotoxic. A myristoylated peptide consisting of residues 31-50 of Nef was neither hemolytic nor cytotoxic. Alteration of the tryptophan residue at position 13 to a serine did not change the hemolytic and cytotoxic activity. Studies of the ultraviolet fluorescence of the tryptophan at position 5 in the peptide, using an artificial membrane system and fluorescence-quenching agents that inserted into the bilayer at different levels, suggested that myristoylation results in this residue being brought into contact with the upper hydrocarbon region of the lipid bilayer of the cell membrane. This tryptophan is flanked by a number of polar residues that would maintain it in this position, resulting in a considerable increase in disorder in the upper regions of the lipid bilayer, leading to its destabilization and to lysis. The cytotoxic activity of the myristoylated Nef fragments may, in part, explain the killing and deletion of cells, especially in lymphoid tissues, during HIV infection.
Subject(s)
Cytotoxicity, Immunologic/drug effects , Gene Products, nef/pharmacology , HIV-1 , Oligopeptides/pharmacology , Amino Acid Sequence , Cell Line/drug effects , Cytotoxicity Tests, Immunologic , HIV-1/chemistry , Hemolysis/drug effects , Humans , Lipid Bilayers/chemistry , Liposomes , Molecular Sequence Data , Myristic Acids/metabolism , Oligopeptides/chemical synthesis , Spectrometry, Fluorescence , nef Gene Products, Human Immunodeficiency VirusABSTRACT
The biological effects of the HIV-1 accessory protein, Vpr, have been studied in yeast expression systems. In our previous study [1], employing the pCUP1-vpr copper-inducible expression cassette, Vpr was shown to cause growth arrest and structural defects. In this study yeast constitutively expressing vpr, through elevated copy number and/or elevated transcription levels, displayed no growth arrest in fermentative growth conditions while Vpr was produced at much lower levels than in the inducible expression system. However, such cells were respiratory deficient and unable to utilise ethanol or glycerol as the sole carbon source. They exhibited gross mitochondrial dysfunction displayed in the loss of respiratory chain complex I, II, III, IV and citrate synthase activities. The effects on mitochondria required a C-terminal domain of Vpr that contains a conserved amino acid sequence motif HFRIGCRHSRIG. These results suggest that the widely observed phenomenon of 'Vpr-induced growth arrest' in human cells could be due to mitochondrial dysfunction.
Subject(s)
Gene Products, vpr/physiology , HIV-1 , Mitochondria/metabolism , Saccharomyces cerevisiae/growth & development , Gene Products, vpr/biosynthesis , Gene Products, vpr/genetics , Glutathione Transferase/genetics , Humans , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/pharmacology , Saccharomyces cerevisiae/metabolism , Transfection , vpr Gene Products, Human Immunodeficiency VirusABSTRACT
The Nef protein of human immunodeficiency type 1 (HIV-1) has been implicated in diverse intracellular functions; however, extracellular functions have been less studied. Nef and the N-terminus of Nef possess membrane-perturbing and fusogenic activities in artificial membranes that also cause cytotoxicity to human cells, including lymphocytes. The present study investigates the toxicity of HIV-1 Nef peptides employing yeast and bacterial cells. The N-terminal portion of Nef was found to cause cell killing in Escherichia coli and in a variety of yeast cells. This activity was enhanced by myristylation of the Nef N-terminus, a modification that did not lead to toxicity in a control peptide. Cell death in yeast was due to permeabilization of the cell membrane as determined by the propidium iodide uptake of peptide-treated cells. Extracellular Nef, or its breakdown products, may have effects similar to the Nef peptides described here and could be responsible, at least in part, for the death of cells in lymphoid tissues during AIDS. Assays using yeast or bacteria are convenient, inexpensive, and robust and should be useful in further analysis and screening of inhibitors of this activity associated with HIV-1 Nef.
Subject(s)
Gene Products, nef/pharmacology , HIV-1/pathogenicity , Peptide Fragments/pharmacology , Amino Acid Sequence , Candida/drug effects , Candida albicans/drug effects , Cell Membrane Permeability/drug effects , Escherichia coli/drug effects , Gene Products, nef/genetics , HIV-1/genetics , Humans , Kluyveromyces/genetics , Molecular Sequence Data , Peptide Fragments/genetics , Saccharomyces cerevisiae/drug effects , nef Gene Products, Human Immunodeficiency VirusABSTRACT
The gene encoding phenylethanolamine N-methyltransferase (PNMT) has been amplified from a human adrenal medulla cDNA library. Following ligation of the gene into a pET3a-derived expression vector and transformation into Escherichia coli BL21(DE3)pLysS, PNMT was expressed, yielding about 10% of the soluble protein. The enzyme was purified to homogeneity by ammonium sulfate fractionation followed by ion-exchange chromatography and gel filtration. The Km for phenylethanolamine and S-adenosyl-L-methionine were determined to be 130 and 16 microM, respectively. The enzyme could be inhibited by reagents expected to modify cysteine, arginine, tyrosine, and histidine residues, but not by methyl acetimidate, a reagent expected to modify lysine residues.
Subject(s)
Phenylethanolamine N-Methyltransferase/genetics , Recombinant Proteins/genetics , Amino Acid Sequence , Chromatography, Ion Exchange , Cloning, Molecular , DNA Primers , Diethyl Pyrocarbonate/pharmacology , Electrophoresis, Polyacrylamide Gel , Escherichia coli/genetics , Gene Expression/genetics , Humans , Imidoesters/pharmacology , Iodoacetamide/pharmacology , Kinetics , Methyl Methanesulfonate/analogs & derivatives , Methyl Methanesulfonate/pharmacology , Molecular Sequence Data , Phenylethanolamine N-Methyltransferase/chemistry , Phenylethanolamine N-Methyltransferase/isolation & purification , Phenylglyoxal/pharmacology , Plasmids/genetics , Recombinant Proteins/isolation & purification , S-Adenosylmethionine/metabolism , Tetranitromethane/pharmacologyABSTRACT
Vpr is a virion-associated protein of human immunodeficiency virus type 1 (HIV-1) whose function in acquired immune deficiency syndrome (AIDS) has been uncertain. We previously employed yeast as a model to examine the effects of Vpr on basic cellular functions; intracellular Vpr was shown to cause cell-growth arrest and structural defects, and these effects were caused by a region of Vpr containing the sequence HFRIGCRHSRIG. Here we show that peptides containing the H(S/F)RIG amino acid sequence motif cause death when added externally to a variety of yeast including Saccharomyces cerevisiae, Kluyveromyces lactis, Candida glabrata, Candida albicans and Schizosaccharomyces pombe. Such peptides rapidly entered the cell from the time of addition, resulting in cell death. Elevated levels of ions, particularly magnesium and calcium ions, abrogated the cytotoxic effect by preventing the Vpr peptides from entering the cells. Extracellular Vpr found in the serum, or breakdown products of extracellular Vpr, may have similar effects to the Vpr peptides described here and could explain the death of uninfected bystander cells during AIDS.
Subject(s)
Gene Products, vpr/genetics , HIV-1/genetics , Amino Acid Sequence , Cell Division/drug effects , Cell Membrane Permeability/drug effects , Gene Products, vpr/physiology , HIV-1/pathogenicity , HIV-1/physiology , Humans , Hydrogen-Ion Concentration , Molecular Sequence Data , Peptide Fragments/genetics , Peptide Fragments/pharmacology , Yeasts/cytology , Yeasts/drug effects , Yeasts/metabolism , vpr Gene Products, Human Immunodeficiency VirusABSTRACT
We have produced human immunodeficiency virus type 1 (HIV-1) Nef (a myristylated 206-amino-acid protein) in Saccharomyces cerevisaie and shown that, while Nef is normally found as a predominantly intracellular protein, amounts up to 40 micrograms/ml of Nef are also released into the extracellular medium during stress. By electrophoretic (SDS-PAGE) analysis the extracellular Nef is indistinguishable from intracellular Nef. Conditions of stress that lead to the release of Nef include elevated levels of copper or magnesium ions or growth at elevated temperatures. This release appears to be dependent upon the N-terminal sequences of Nef, including the presence of a myristylation site. Our observations concerning Nef release in yeast suggest new ways in which the behaviour of Nef should be examined in order to gain further insights into the development of AIDS. If the release of Nef is important in the development of AIDS, our work reveals that Nef-associated symptoms may be reduced or delayed by reducing stresses, such as fevers.
Subject(s)
Gene Products, nef/metabolism , HIV-1/chemistry , Recombinant Proteins/metabolism , Saccharomyces cerevisiae/metabolism , Amino Acid Sequence , Base Sequence , Copper/pharmacology , Gene Products, nef/chemistry , Hot Temperature , Magnesium/pharmacology , Molecular Sequence Data , Myristic Acid , Myristic Acids/metabolism , Protein Processing, Post-Translational , nef Gene Products, Human Immunodeficiency VirusABSTRACT
Vpr is a virion-associated protein of human immunodeficiency type 1 (HIV-1) whose function in acquired immunodeficiency syndrome (AIDS) has been uncertain. Employing the yeast Saccharomyces cerevisiae as a model to examine the effects of HIV-1 auxiliary proteins on basic cellular functions, we found that the vpr gene caused cell growth arrest and structural defects indicated by osmotic sensitivity and gross cell enlargement. Production of various domains by gene expression showed that this effect arose from within the carboxyl-terminal third of the Vpr protein and implicated the sequence HFRIGCRHSRIG, containing two H(S/F)RIG motifs. Electroporation with a series of peptides containing these motifs caused structural defects in yeast that resulted in osmotic sensitivity. A protein with functions relating to the yeast cytoskeleton, Sac1p [Cleves, A. E., Novick, P.J. & Bankaitis, V.A. (1989) J. Cell Biol. 109, 2939-2950], shows sequence similarity to Vpr, and Vpr's effect in yeast may be to disrupt normal Sac1p functions. The Sac1p equivalent has not yet been described in mammalian cells, but in rhabdomyosarcoma and osteosarcoma cell lines Vpr also caused gross cell enlargement and replication arrest [Levy, D.N., Fernandes, L.S., Williams, W.V. & Weiner, D.B. (1993) Cell 72, 541-550]. We note that there is a correlation between the region containing the H(S/F)RIG motifs and the pathogenicity of primate lentiviruses and we suggest that the function of Vpr may be to bring about cell growth arrest and/or cytoskeletal changes as an early step in HIV-1 infection.
Subject(s)
Gene Products, vpr/chemistry , Gene Products, vpr/pharmacology , HIV-1/genetics , Saccharomyces cerevisiae/growth & development , Acquired Immunodeficiency Syndrome/virology , Amino Acid Sequence , Base Sequence , Cell Division/drug effects , Cloning, Molecular , Flow Cytometry , Gene Products, vpr/biosynthesis , Genes, vpr , HIV-1/metabolism , HIV-1/pathogenicity , Humans , Molecular Sequence Data , Peptide Fragments/chemical synthesis , Peptide Fragments/chemistry , Peptide Fragments/pharmacology , Polymerase Chain Reaction , Saccharomyces cerevisiae/cytology , Sequence Homology, Amino Acid , vpr Gene Products, Human Immunodeficiency VirusABSTRACT
Influenza NS2 protein was expressed in Saccharomyces cerevisiae using a copper-inducible promoter. The protein produced had a molecular weight of 13 kDa, was reactive with anti-NS2 antiserum and was localised to the yeast cell nucleus. Two-hybrid analysis identified a direct protein-protein interaction between NS2 and the M2 protein of the virus, involving the C-terminal 163 residues of M1. A filter-binding assay localised the M1 binding region to the C-terminal 70 amino acids of NS2.
Subject(s)
Influenza A virus/metabolism , Viral Matrix Proteins/metabolism , Viral Nonstructural Proteins/metabolism , Amino Acid Sequence , Base Sequence , Cloning, Molecular , DNA Primers/chemistry , Influenza A virus/genetics , Molecular Sequence Data , Protein Binding , Recombinant Proteins , Saccharomyces cerevisiae , Viral Nonstructural Proteins/geneticsABSTRACT
We have examined heterologous protein secretion from Candida glabrata with the aid of a stable C. glabrata vector and a secretion reporter cassette comprising the Saccharomyces cerevisiae PGK-gene promoter and a Kluveromyces Iactis secretion signal to drive secretion of Escherichia coli beta-lactamase. Abundant secretion of beta-lactamase from C. glabrata indicates that the S. cerevisiae PGK promoter functions in C. glabrata. Furthermore, we show that C. glabrata processes the secreted beta-lactamase in a manner similar to, but not identical with, S. cerevisiae and K. lactis. C. glabrata may be a suitable new host for the expression of foreign genes.
Subject(s)
Candida/genetics , Recombinant Proteins/genetics , Amino Acid Sequence , Base Sequence , Candida/metabolism , Cloning, Molecular , DNA, Recombinant , Molecular Sequence Data , Phosphoglycerate Kinase/genetics , Plasmids , Promoter Regions, Genetic , Recombinant Proteins/metabolism , Saccharomyces cerevisiae/genetics , beta-Lactamases/metabolismABSTRACT
We have sought to obtain a convenient system for the high-level production of secreted proteins in yeast. With the aid of a secretion reporter cassette we examined the secretion of beta-lactamase (Bla) as a model protein and found the highest expression in Saccharomyces cerevisiae using a high-copy-number plasmid. We further developed the high-copy-number plasmid introducing a secretion cassette that has a convenient cloning site coinciding with the sequence encoding the KEX2 cleavage site. Large quantities of correctly-processed product can therefore be obtained. We show that 0.3 g/l of correctly processed beta-lactamase can be obtained in fed-batch cultures without the need for selective media or significant loss of the plasmid.
Subject(s)
Genetic Vectors , Plasmids , Protein Processing, Post-Translational , Saccharomyces cerevisiae/enzymology , beta-Lactamases/metabolism , Amino Acid Sequence , Base Sequence , Cloning, Molecular , Culture Media , DNA, Fungal , Molecular Sequence Data , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/growth & development , beta-Lactamases/geneticsABSTRACT
We describe six new yeast episomal vectors which encode glutathione S-transferase (GST) affinity tags. These allow for the production of GST-fusion proteins in Saccharomyces cerevisiae under the control of the CUP1 promoter. Affinity chromatography with glutathione-Sepharose permits convenient purification of the fusion protein from a yeast lysate. The presence of a protease cleavage site facilitates subsequent removal of the GST tag. The expression and single-step purification of both GST and a functional GST-metallothionein fusion from yeast are shown as an example of the application of these vectors.
Subject(s)
Chromatography, Affinity , Copper/pharmacology , Genetic Vectors , Glutathione Transferase/isolation & purification , Promoter Regions, Genetic , Protozoan Proteins/genetics , Recombinant Fusion Proteins/isolation & purification , Saccharomyces cerevisiae/metabolism , Schistosoma japonicum/enzymology , Amino Acid Sequence , Animals , Base Sequence , Enzyme Induction/drug effects , Escherichia coli/metabolism , Factor Xa/genetics , Gene Expression Regulation, Fungal/drug effects , Glutathione Transferase/biosynthesis , Glutathione Transferase/genetics , Metallothionein/genetics , Molecular Sequence Data , Recombinant Fusion Proteins/biosynthesis , Recombinant Fusion Proteins/genetics , Schistosoma japonicum/genetics , Thrombin/geneticsABSTRACT
The influenza A virus non-structural protein NS1 was produced using a copper-inducible expression system in the yeast Saccharomyces cerevisiae. The protein produced had a molecular weight of 26 kDa by SDS-PAGE and was reactive with anti-NS1 antisera. The recombinant NS1 protein was targetted to the nucleolus/nuclear envelope fraction of the yeast cell nucleus, showing that its localisation signals remain functional in yeast. In addition, immune-electron microscopy detected cytoplasmic inclusions reminiscent of those seen in cells infected with some influenza strains. The NS1 protein was shown to be capable of in vivo self-interaction which probably forms the basis of its propensity to form inclusions. Expression of the protein was found to be toxic to yeast cells expressing it, supporting a role for the protein in the shutdown of influenza virus-infected cells. Deletion mapping of NS1 pointed to 2 regions of the molecule being important for this toxicity: a basic C-terminal stretch which has been shown to act as a nuclear localisation signal, and an N-terminal region implicated in RNA binding.