ABSTRACT
Huntington's disease (HD) is caused by an expanded CAG trinucleotide repeat in exon 1 of the huntingtin (HTT) gene. We report the design of a series of HTT pre-mRNA splicing modulators that lower huntingtin (HTT) protein, including the toxic mutant huntingtin (mHTT), by promoting insertion of a pseudoexon containing a premature termination codon at the exon 49-50 junction. The resulting transcript undergoes nonsense-mediated decay, leading to a reduction of HTT mRNA transcripts and protein levels. The starting benzamide core was modified to pyrazine amide and further optimized to give a potent, CNS-penetrant, and orally bioavailable HTT-splicing modulator 27. This compound reduced canonical splicing of the HTT RNA exon 49-50 and demonstrated significant HTT-lowering in both human HD stem cells and mouse BACHD models. Compound 27 is a structurally diverse HTT-splicing modulator that may help understand the mechanism of adverse effects such as peripheral neuropathy associated with branaplam.
ABSTRACT
Pathological hallmarks of amyotrophic lateral sclerosis (ALS), including protein misfolding, are well established in oligodendrocytes. More recently, an RNA trafficking deficit of key myelin proteins has been suggested in oligodendrocytes in ALS but the extent to which this affects myelination and the relative contribution of this to disease pathogenesis is unclear. ALS autopsy research findings showing demyelination contrasts with the routine clinical-pathological workup of ALS cases where it is rare to see white matter abnormalities other than simple Wallerian degeneration secondary to widespread neuronal loss. To begin to address this apparent variance, we undertook a comprehensive evaluation of myelination at an RNA, protein and structural level using human pathological material from sporadic ALS patients, genetic ALS patients (harboring C9orf72 mutation) and age- and sex-matched non-neurological controls. We performed (i) quantitative spatial profiling of the mRNA transcript encoding myelin basic protein (MBP), (ii) quantification of MBP protein and (iii) the first quantitative structural assessment of myelination in ALS post-mortem specimens by electron microscopy. We show no differences in MBP protein levels or ultrastructural myelination, despite a significant dysregulation in the subcellular trafficking of MBP mRNA in ALS patients compared to controls. We therefore confirm that whilst there are cell autonomous mRNA trafficking deficits affecting oligodendrocytes in ALS, this has no effect on myelin structure.
ABSTRACT
Myelination is essential for central nervous system (CNS) formation, health, and function. Emerging evidence of oligodendrocyte heterogeneity in health and disease and divergent CNS gene expression profiles between mice and humans supports the development of experimentally tractable human myelination systems. Here, we developed human iPSC-derived myelinating organoids ("myelinoids") and quantitative tools to study myelination from oligodendrogenesis through to compact myelin formation and myelinated axon organization. Using patient-derived cells, we modeled a monogenetic disease of myelinated axons (Nfasc155 deficiency), recapitulating impaired paranodal axo-glial junction formation. We also validated the use of myelinoids for pharmacological assessment of myelination-both at the level of individual oligodendrocytes and globally across whole myelinoids-and demonstrated reduced myelination in response to suppressed synaptic vesicle release. Our study provides a platform to investigate human myelin development, disease, and adaptive myelination.
Subject(s)
Induced Pluripotent Stem Cells/cytology , Myelin Sheath/physiology , Organoids/physiology , Axons/metabolism , Axons/ultrastructure , Humans , Myelin Sheath/ultrastructure , Nerve Growth Factors/deficiency , Nerve Growth Factors/metabolism , Organoids/ultrastructure , Tetanus Toxin/pharmacology , Time FactorsABSTRACT
Oligodendrocytes are implicated in amyotrophic lateral sclerosis pathogenesis and display transactive response DNA-binding protein-43 (TDP-43) pathological inclusions. To investigate the cell autonomous consequences of TDP-43 mutations on human oligodendrocytes, we generated oligodendrocytes from patient-derived induced pluripotent stem cell lines harbouring mutations in the TARDBP gene, namely G298S and M337V. Through a combination of immunocytochemistry, electrophysiological assessment via whole-cell patch clamping, and three-dimensional cultures, no differences in oligodendrocyte differentiation, maturation or myelination were identified. Furthermore, expression analysis for monocarboxylate transporter 1 (a lactate transporter) coupled with a glycolytic stress test showed no deficit in lactate export. However, using confocal microscopy, we report TDP-43 mutation-dependent pathological mis-accumulation of TDP-43. Furthermore, using in vitro patch-clamp recordings, we identified functional Ca2+-permeable α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor dysregulation in oligodendrocytes. Together, these findings establish a platform for further interrogation of the role of oligodendrocytes and cellular autonomy in TDP-43 proteinopathy.
ABSTRACT
Neuroglial precursor cells (NPC) possess immune-modulatory properties by which they prevent immune-mediated injury in experimental autoimmune encephalomyelitis (EAE). It is unclear whether cell transplantation in a clinical-relevant setup induces ongoing therapeutic effects in a chronic-active model of progressive multiple sclerosis (MS). We examined whether human embryonic stem cell (hESC)-derived NPCs inhibit progressive EAE in Biozzi AB/H mice, manifesting with chronic-active neuroinflammation and demyelinated plaques. hESC-derived NPCs were propagated for 6-8 weeks as spheres enriched for Olig2+ cells to switch from neuronal to glial commitment and to enrich for oligodendrocyte progenitor cells. NPC were transplanted intracerebroventricularly at 30 days post-EAE induction, after the acute relapse. We evaluated effects of cell transplantation on clinical parameters, neuroinflammation, myelination, and axonal loss. Transplanted animals exhibited a significantly milder disease, reduced neuroinflammation, reduced demyelination, and reduced axonal loss as compared to control EAE mice. Toluidine-blue semi-thin staining showed a bystander neuroprotective effect of human precursor cells preventing the loss of myelinated fibers in superficial layer of the cervical dorsal funiculus. Human Olig2+ cells were detected along spinal cord meninges after 65 days of follow-up. In co-cultures in vitro, Olig2+ human precursors inhibited Concanavalin A-induced murine T cell activation and proliferation. To conclude, glial-committed human NPC induce ongoing immune-regulatory and neuroprotective effects, following transplantation into mice with a clinical-relevant model of chronic-active MS and during established disease, entering the chronic phase. These properties highlight the therapeutic potential of human NPC transplantation in chronic MS and their delivery via the cerebrospinal fluid.
Subject(s)
Multiple Sclerosis/immunology , Multiple Sclerosis/therapy , Neural Stem Cells/transplantation , Oligodendrocyte Precursor Cells/cytology , Animals , Encephalomyelitis, Autoimmune, Experimental/immunology , Humans , Mice , Myelin Sheath/immunology , Neurons/cytology , Oligodendrocyte Transcription Factor 2/metabolism , Stem Cell Transplantation/methodsABSTRACT
Although the underlying neurobiology of major mental illness (MMI) remains unknown, emerging evidence implicates a role for oligodendrocyte-myelin abnormalities. Here, we took advantage of a large family carrying a balanced t(1;11) translocation, which substantially increases risk of MMI, to undertake both diffusion tensor imaging and cellular studies to evaluate the consequences of the t(1;11) translocation on white matter structural integrity and oligodendrocyte-myelin biology. This translocation disrupts among others the DISC1 gene which plays a crucial role in brain development. We show that translocation-carrying patients display significant disruption of white matter integrity compared with familial controls. At a cellular level, we observe dysregulation of key pathways controlling oligodendrocyte development and morphogenesis in induced pluripotent stem cell (iPSC) derived case oligodendrocytes. This is associated with reduced proliferation and a stunted morphology in vitro. Further, myelin internodes in a humanized mouse model that recapitulates the human translocation as well as after transplantation of t(1;11) oligodendrocyte progenitors were significantly reduced when compared with controls. Thus we provide evidence that the t(1;11) translocation has biological effects at both the systems and cellular level that together suggest oligodendrocyte-myelin dysfunction.
Subject(s)
Myelin Sheath/metabolism , Oligodendroglia/metabolism , Translocation, Genetic/genetics , Adult , Animals , Chromosomes, Human, Pair 1/genetics , Chromosomes, Human, Pair 11/genetics , Diffusion Tensor Imaging/methods , Female , Humans , Induced Pluripotent Stem Cells/metabolism , Male , Mental Disorders/genetics , Mice , Middle Aged , Nerve Tissue Proteins/genetics , Nerve Tissue Proteins/metabolism , White Matter/metabolism , White Matter/physiologyABSTRACT
The in vitro generation of defined cellular populations from induced human pluripotent stem cells (iPSCs) provides the opportunity to work routinely with human material and, importantly, allows examination of material derived from patients with clinically and genetically diagnosed disorders. In this regard, the ability to derive oligodendrocytes in vitro represents an important resource to examine human oligodendrocyte-lineage cell biology in normal and disease contexts. Oligodendrocytes undergo characteristic physiological maturation during differentiation in vitro, and patch-clamp electrophysiology allows a detailed examination of maturation state and, potentially, pathologically related variations of ion channel expression and regulation. Here, we detail our methodology to generate oligodendrocyte precursor cells and oligodendrocytes and characterize them electrophysiologically.
Subject(s)
Cell Culture Techniques/methods , Induced Pluripotent Stem Cells/cytology , Oligodendroglia/cytology , Cell Differentiation , Cell Lineage , Cells, Cultured , Electrophysiological Phenomena , Humans , Patch-Clamp TechniquesABSTRACT
Mutations in C9ORF72 are the most common cause of familial amyotrophic lateral sclerosis (ALS). Here, through a combination of RNA-Seq and electrophysiological studies on induced pluripotent stem cell (iPSC)-derived motor neurons (MNs), we show that increased expression of GluA1 AMPA receptor (AMPAR) subunit occurs in MNs with C9ORF72 mutations that leads to increased Ca2+-permeable AMPAR expression and results in enhanced selective MN vulnerability to excitotoxicity. These deficits are not found in iPSC-derived cortical neurons and are abolished by CRISPR/Cas9-mediated correction of the C9ORF72 repeat expansion in MNs. We also demonstrate that MN-specific dysregulation of AMPAR expression is also present in C9ORF72 patient post-mortem material. We therefore present multiple lines of evidence for the specific upregulation of GluA1 subunits in human mutant C9ORF72 MNs that could lead to a potential pathogenic excitotoxic mechanism in ALS.
Subject(s)
C9orf72 Protein/genetics , Motor Neurons/pathology , Receptors, AMPA/metabolism , Amyotrophic Lateral Sclerosis/genetics , C9orf72 Protein/metabolism , CRISPR-Cas Systems , Calcium/metabolism , DNA Repeat Expansion , Gene Targeting , Humans , Receptors, AMPA/genetics , Spinal Cord/metabolism , Spinal Cord/physiopathologyABSTRACT
Evolutionary differences in gene regulation between humans and lower mammalian experimental systems are incompletely understood, a potential translational obstacle that is challenging to surmount in neurons, where primary tissue availability is poor. Rodent-based studies show that activity-dependent transcriptional programs mediate myriad functions in neuronal development, but the extent of their conservation in human neurons is unknown. We compared activity-dependent transcriptional responses in developing human stem cell-derived cortical neurons with those induced in developing primary- or stem cell-derived mouse cortical neurons. While activity-dependent gene-responsiveness showed little dependence on developmental stage or origin (primary tissue vs. stem cell), notable species-dependent differences were observed. Moreover, differential species-specific gene ortholog regulation was recapitulated in aneuploid mouse neurons carrying human chromosome-21, implicating promoter/enhancer sequence divergence as a factor, including human-specific activity-responsive AP-1 sites. These findings support the use of human neuronal systems for probing transcriptional responses to physiological stimuli or indeed pharmaceutical agents.
Subject(s)
Biological Evolution , Gene Expression Regulation, Developmental , Neural Stem Cells/physiology , Neurons/physiology , Transcription, Genetic , Animals , Cells, Cultured , Humans , MiceABSTRACT
Rodent-based studies have shown that the membrane properties of oligodendrocytes play prominent roles in their physiology and shift markedly during their maturation from the oligodendrocyte precursor cell (OPC) stage. However, the conservation of these properties and maturation processes in human oligodendrocytes remains unknown, despite their dysfunction being implicated in human neurodegenerative diseases such as multiple sclerosis (MS) and amyotrophic lateral sclerosis (ALS). Here, we have defined the membrane properties of human oligodendrocytes derived from pluripotent stem cells as they mature from the OPC stage, and have identified strong conservation of maturation-specific physiological characteristics reported in rodent systems. We find that as human oligodendrocytes develop and express maturation markers, they exhibit a progressive decrease in voltage-gated sodium and potassium channels and a loss of tetrodotoxin-sensitive spiking activity. Concomitant with this is an increase in inwardly rectifying potassium channel activity, as well as a characteristic switch in AMPA receptor composition. All these steps mirror the developmental trajectory observed in rodent systems. Oligodendrocytes derived from mutant C9ORF72-carryng ALS patient induced pluripotent stem cells did not exhibit impairment to maturation and maintain viability with respect to control lines despite the presence of RNA foci, suggesting that maturation defects may not be a primary feature of this mutation. Thus, we have established that the development of human oligodendroglia membrane properties closely resemble those found in rodent cells and have generated a platform to enable the impact of human neurodegenerative disease-causing mutations on oligodendrocyte maturation to be studied.
Subject(s)
Amyotrophic Lateral Sclerosis/physiopathology , Cell Differentiation/genetics , Multiple Sclerosis/physiopathology , Oligodendroglia/physiology , Pluripotent Stem Cells/physiology , Amyotrophic Lateral Sclerosis/genetics , Amyotrophic Lateral Sclerosis/pathology , Animals , Electrophysiological Phenomena , Female , Humans , Male , Multiple Sclerosis/genetics , Mutation , Neurogenesis/genetics , Neurogenesis/physiology , Oligodendroglia/pathology , Pluripotent Stem Cells/pathology , Potassium Channels, Voltage-Gated/genetics , Voltage-Gated Sodium Channels/geneticsABSTRACT
The mammalian eminentia thalami (EmT) (or thalamic eminence) is an embryonic forebrain structure of unknown function. Here, we examined the molecular and cellular properties of the mouse EmT. We first studied mRNA expression of signalling molecules and found that the EmT is a structure, rich in expression of secreted factors, with Wnts being the most abundantly detected. We then examined whether EmT tissue could induce cell fate changes when grafted ectopically. For this, we transplanted EmT tissue from a tau-GFP mouse to the ventral telencephalon of a wild type host, a telencephalic region where Wnt signalling is not normally active but which we showed in culture experiments is competent to respond to Wnts. We observed that the EmT was able to induce in adjacent ventral telencephalic cells ectopic expression of Lef1, a transcriptional activator and a target gene of the Wnt/ß-catenin pathway. These Lef1-positive;GFP-negative cells expressed the telencephalic marker Foxg1 but not Ascl1, which is normally expressed by ventral telencephalic cells. These results suggest that the EmT has the capacity to activate Wnt/ß-catenin signalling in the ventral telencephalon and to suppress ventral telencephalic gene expression. Altogether, our data support a role of the EmT as a signalling centre in the developing mouse forebrain.
Subject(s)
Gene Expression Regulation, Developmental , Prosencephalon/embryology , Prosencephalon/metabolism , Thalamus/embryology , Thalamus/metabolism , Wnt Signaling Pathway , Animals , Basic Helix-Loop-Helix Transcription Factors/metabolism , Bone Morphogenetic Protein 4 , Bone Morphogenetic Protein 6 , Cells, Cultured , Fibroblast Growth Factor 8 , Fibroblast Growth Factors , Mice , RNA, Messenger/metabolism , Signal TransductionABSTRACT
The in vitro derivation of regionally defined human neuron types from patient-derived stem cells is now established as a resource to investigate human development and disease. Characterization of such neurons initially focused on the expression of developmentally regulated transcription factors and neural markers, in conjunction with the development of protocols to direct and chart the fate of differentiated neurons. However, crucial to the understanding and exploitation of this technology is to determine the degree to which neurons recapitulate the key functional features exhibited by their native counterparts, essential for determining their usefulness in modelling human physiology and disease in vitro. Here, we review the emerging data concerning functional properties of human pluripotent stem cell-derived excitatory cortical neurons, in the context of both maturation and regional specificity.
Subject(s)
Action Potentials/physiology , Pluripotent Stem Cells/physiology , Animals , Cell Differentiation/physiology , Humans , Neurons , Pluripotent Stem Cells/metabolism , Transcription Factors/metabolismABSTRACT
Primary cilia are complex subcellular structures that play key roles during embryogenesis by controlling the cellular response to several signaling pathways. Defects in the function and/or structure of primary cilia underlie a large number of human syndromes collectively referred to as ciliopathies. Often, ciliopathies are associated with mental retardation (MR) and malformation of the corpus callosum. However, the possibility of defects in other forebrain axon tracts, which could contribute to the cognitive disorders of these patients, has not been explored. Here, we investigate the formation of the corticothalamic/thalamocortical tracts in mice mutant for Rfx3, which regulates the expression of many genes involved in ciliogenesis and cilia function. Using DiI axon tracing and immunohistochemistry experiments, we show that some Rfx3(-/-) corticothalamic axons abnormally migrate toward the pial surface of the ventral telencephalon (VT). Some thalamocortical axons (TCAs) also fail to leave the diencephalon or abnormally project toward the amygdala. Moreover, the Rfx3(-/-) VT displays heterotopias containing attractive guidance cues and expressing the guidance molecules Slit1 and Netrin1. Finally, the abnormal projection of TCAs toward the amygdala is also present in mice carrying a mutation in the Inpp5e gene, which is mutated in Joubert Syndrome and which controls cilia signaling and stability. The presence of identical thalamocortical malformations in two independent ciliary mutants indicates a novel role for primary cilia in the formation of the corticothalamic/thalamocortical tracts by establishing the correct cellular environment necessary for its development.
Subject(s)
Body Patterning/genetics , Cerebral Cortex/metabolism , DNA-Binding Proteins/genetics , Telencephalon/metabolism , Thalamus/metabolism , Transcription Factors/genetics , Animals , Embryo, Mammalian , Homozygote , Immunohistochemistry , Kruppel-Like Transcription Factors/metabolism , Mice , Mutation , Nerve Tissue Proteins/metabolism , Neural Pathways , Neurons/metabolism , Phosphoric Monoester Hydrolases/genetics , Regulatory Factor X Transcription Factors , Telencephalon/embryology , Telencephalon/pathology , Thalamus/embryology , Thalamus/pathology , Zinc Finger Protein Gli3ABSTRACT
The corticothalamic and thalamocortical tracts play essential roles in the communication between the cortex and thalamus. During development, axons forming these tracts have to follow a complex path to reach their target areas. While much attention has been paid to the mechanisms regulating their passage through the ventral telencephalon, very little is known about how the developing cortex contributes to corticothalamic/thalamocortical tract formation. Gli3 encodes a zinc finger transcription factor widely expressed in telencephalic progenitors which has important roles in corticothalamic and thalamocortical pathfinding. Here, we conditionally inactivated Gli3 in dorsal telencephalic progenitors to determine its role in corticothalamic tract formation. In Emx1Cre;Gli3(fl/fl) mutants, only a few corticothalamic axons enter the striatum in a restricted dorsal domain. This restricted entry correlates with a medial expansion of the piriform cortex. Transplantation experiments showed that the expanded piriform cortex repels corticofugal axons. Moreover, expression of Sema5B, a chemorepellent for corticofugal axons produced by the piriform cortex, is similarly expanded. Finally, time course analysis revealed an expansion of the ventral pallial progenitor domain which gives rise to the piriform cortex. Hence, control of lateral cortical development by Gli3 at the progenitor level is crucial for corticothalamic pathfinding.
Subject(s)
Axons/physiology , Kruppel-Like Transcription Factors/metabolism , Nerve Tissue Proteins/metabolism , Piriform Cortex/embryology , Piriform Cortex/physiopathology , Thalamus/embryology , Thalamus/physiopathology , Animals , Axons/pathology , Corpus Striatum/embryology , Corpus Striatum/pathology , Corpus Striatum/physiopathology , Immunohistochemistry , In Situ Hybridization , Kruppel-Like Transcription Factors/genetics , Mice, Transgenic , Mutation , Nerve Tissue Proteins/genetics , Neural Pathways/embryology , Neural Pathways/pathology , Neural Pathways/physiopathology , Piriform Cortex/pathology , Semaphorins/metabolism , Thalamus/pathology , Tissue Culture Techniques , Zinc Finger Protein Gli3ABSTRACT
Lizards are soil surface animals that represent an important link between invertebrates and higher predators. Being part of wild fauna, they can be affected by contamination from anthropic activities and in particular, pesticides and chemical substances of various nature that reach the soil surface directly or through fall out. Among these substances, heavy metals such as cadmium may exert particularly marked toxic effect on both adult and embryos. In lizards, recent studies show that cadmium may cause developmental defects, including alteration of eye development, with appearance of unilateral microphthalmia and retinal folding. In the present study, the effects of cadmium incubation on retinal development were investigated demonstrating that cadmium interferes with cell cycle regulation by increasing proliferation. An increased expression of Otx2 and Pax6 genes, markers of retinal differentiation, was also found. However, the cellular localization of Pax6 and Otx2 transcripts did not change in treated embryos: in the early stages of retinogenesis, the two genes were expressed in all retinal cells; in the differentiated retina, Otx2 remained in the cellular bodies of retinal cells forming the nuclear and the ganglion layers, whereas Pax6 was expressed only in the cells of the inner nuclear and the ganglion layers. Data suggest that the increased expression of Pax6 and Otx2 could be ascribed to the hyperproliferation of retinal cells rather than to an effective gene overexpression.
Subject(s)
Cadmium/toxicity , Retina/growth & development , Soil Pollutants/toxicity , Animals , Cell Differentiation/drug effects , Gene Expression Regulation, Developmental/drug effects , Lizards/embryology , Neurons/drug effects , RNA, Messenger/biosynthesis , Retina/drug effects , Retina/embryologyABSTRACT
The corpus callosum (CC) represents the major forebrain commissure connecting the 2 cerebral hemispheres. Midline crossing of callosal axons is controlled by several glial and neuronal guideposts specifically located along the callosal path, but it remains unknown how these cells acquire their position. Here, we show that the Gli3 hypomorphic mouse mutant Polydactyly Nagoya (Pdn) displays agenesis of the CC and mislocation of the glial and neuronal guidepost cells. Using transplantation experiments, we demonstrate that agenesis of the CC is primarily caused by midline defects. These defects originate during telencephalic patterning and involve an up-regulation of Slit2 expression and altered Fgf and Wnt/ß-catenin signaling. Mutations in sprouty1/2 which mimic the changes in these signaling pathways cause a disorganization of midline guideposts and CC agenesis. Moreover, a partial recovery of midline abnormalities in Pdn/Pdn;Slit2(-/-) embryos mutants confirms the functional importance of correct Slit2 expression levels for callosal development. Hence, Gli3 controlled restriction of Fgf and Wnt/ß-catenin signaling and of Slit2 expression is crucial for positioning midline guideposts and callosal development.
Subject(s)
Corpus Callosum/growth & development , Kruppel-Like Transcription Factors/physiology , Nerve Tissue Proteins/physiology , Telencephalon/growth & development , Agenesis of Corpus Callosum/genetics , Agenesis of Corpus Callosum/physiopathology , Animals , Brain/growth & development , Cluster Analysis , Corpus Callosum/embryology , Female , Immunohistochemistry , In Situ Hybridization , Intercellular Signaling Peptides and Proteins/biosynthesis , Intercellular Signaling Peptides and Proteins/physiology , Kruppel-Like Transcription Factors/genetics , Mice , Mutation/physiology , Nerve Tissue Proteins/biosynthesis , Nerve Tissue Proteins/genetics , Organ Culture Techniques , Polydactyly/genetics , Pregnancy , Real-Time Polymerase Chain Reaction , Receptors, Fibroblast Growth Factor/physiology , Telencephalon/embryology , Up-Regulation/physiology , Wnt Signaling Pathway/physiology , Zinc Finger Protein Gli3 , beta Catenin/physiologyABSTRACT
The corpus callosum (CC) is the largest commissure in the forebrain and mediates the transfer of sensory, motor and cognitive information between the cerebral hemispheres. During CC development, a number of strategically located glial and neuronal guidepost structures serve to guide callosal axons across the midline at the corticoseptal boundary (CSB). Correct positioning of these guideposts requires the Gli3 gene, mutations of which result in callosal defects in humans and mice. However, as Gli3 is widely expressed during critical stages of forebrain development, the precise temporal and spatial requirements for Gli3 function in callosal development remain unclear. Here, we used a conditional mouse mutant approach to inactivate Gli3 in specific regions of the developing telencephalon in order to delineate the domain(s) in which Gli3 is required for normal development of the corpus callosum. Inactivation of Gli3 in the septum or in the medial ganglionic eminence had no effect on CC formation, however Gli3 inactivation in the developing cerebral cortex led to the formation of a severely hypoplastic CC at E18.5 due to a severe disorganization of midline guideposts. Glial wedge cells translocate prematurely and Slit1/2 are ectopically expressed in the septum. These changes coincide with altered Fgf and Wnt/ß-catenin signalling during CSB formation. Collectively, these data demonstrate a crucial role for Gli3 in cortical progenitors to control CC formation and indicate how defects in CSB formation affect the positioning of callosal guidepost cells.
Subject(s)
Corpus Callosum/embryology , Gene Expression Regulation, Developmental , Homeodomain Proteins/genetics , Homeodomain Proteins/physiology , Kruppel-Like Transcription Factors/genetics , Kruppel-Like Transcription Factors/physiology , Nerve Tissue Proteins/genetics , Nerve Tissue Proteins/physiology , Transcription Factors/genetics , Transcription Factors/physiology , Animals , Cell Movement , Crosses, Genetic , Female , Immunohistochemistry/methods , In Situ Hybridization , Male , Mice , Mutation , Signal Transduction , Time Factors , Transgenes , Zinc Finger Protein Gli3ABSTRACT
The formation of a functional cortical circuitry requires the coordinated growth of cortical axons to their target areas. While the mechanisms guiding cortical axons to their targets have extensively been studied, very little is known about the processes which promote their growth in vivo. Gli3 encodes a zinc finger transcription factor which is expressed in cortical progenitor cells and has crucial roles in cortical development. Here, we characterize the Gli3 compound mutant Gli3(Xt/Pdn), which largely lacks Neurofilament(+) fibers in the rostral and intermediate neocortex. DiI labeling and Golli-τGFP immunofluorescence indicate that Gli3(Xt/Pdn) cortical neurons form short and stunted axons. Using transplantation experiments we demonstrate that this axon growth defect is primarily caused by a nonpermissive cortical environment. Furthermore, in Emx1Cre;Gli3(Pdn/fl) conditional mutants, which mimic the reduction of Gli3 expression in the dorsal telencephalon of Gli3(Xt/Pdn) embryos, the growth of cortical axons is not impaired, suggesting that Gli3 controls this process early in telencephalic development. In contrast to cortical plate neurons, Gli3(Xt/Pdn) embryos largely lack subplate (SP) neurons which normally pioneer cortical projections. Collectively, these findings show that Gli3 specifies a cortical environment permissive to the growth of cortical axons at the progenitor level by controlling the formation of SP neurons.
Subject(s)
Axons/physiology , Kruppel-Like Transcription Factors/metabolism , Neocortex/embryology , Nerve Tissue Proteins/metabolism , Neurons/physiology , Animals , Kruppel-Like Transcription Factors/genetics , Mice , Mice, Inbred C57BL , Mutation , Neocortex/metabolism , Nerve Tissue Proteins/genetics , Zinc Finger Protein Gli3ABSTRACT
Regional patterning of the cerebral cortex is initiated by morphogens secreted by patterning centers that establish graded expression of transcription factors within cortical progenitors. Here, we show that Dmrt5 is expressed in cortical progenitors in a high-caudomedial to low-rostrolateral gradient. In its absence, the cortex is strongly reduced and exhibits severe abnormalities, including agenesis of the hippocampus and choroid plexus and defects in commissural and thalamocortical tracts. Loss of Dmrt5 results in decreased Wnt and Bmp in one of the major telencephalic patterning centers, the dorsomedial telencephalon, and in a reduction of Cajal-Retzius cells. Expression of the dorsal midline signaling center-dependent transcription factors is downregulated, including Emx2, which promotes caudomedial fates, while the rostral determinant Pax6, which is inhibited by midline signals, is upregulated. Consistently, Dmrt5(-/-) brains exhibit patterning defects with a dramatic reduction of the caudomedial cortex. Dmrt5 is increased upon the activation of Wnt signaling and downregulated in Gli3(xt/xt) mutants. We conclude that Dmrt5 is a novel Wnt-dependent transcription factor required for early cortical development and that it may regulate initial cortical patterning by promoting dorsal midline signaling center formation and thereby helping to establish the graded expression of the other transcription regulators of cortical identity.