ABSTRACT
A novel regulatory protein, tescalcin (TSC), recently isolated from mouse embryonic testes, has been implicated in gonadal differentiation. Employing the yeast two-hybrid system with the Na(+)/H(+) exchanger type-1 (NHE1) carboxyterminal domain as a bait we have identified a novel NHE1-associated protein of 214 amino acid residues representing the human homolog of mouse TSC (96.7% identity). Co-precipitation experiments demonstrated the interaction of human TSC with NHE1 in vitro and in vivo, and 45Ca(2+) overlay assay revealed that TSC binds Ca(2+). Immunofluorescence studies indicated that TSC is prominent in cellular lamellipodia where it colocalizes with NHE1. Abundant expression of TSC mRNA in the heart suggests that TSC may play important role(s) in concert with NHE1 in cardiac tissues.
Subject(s)
Calcium-Binding Proteins/genetics , Calcium-Binding Proteins/metabolism , Sodium-Hydrogen Exchangers/metabolism , Amino Acid Sequence , Animals , Blotting, Northern , Calcium/metabolism , Calcium-Binding Proteins/immunology , Humans , Mice , Molecular Sequence Data , Myocardium/metabolism , Sequence Homology, Amino Acid , Sodium-Hydrogen Exchangers/genetics , Sodium-Hydrogen Exchangers/immunology , Two-Hybrid System TechniquesABSTRACT
Paget's disease of bone can occur in up to 4% of the population over forty. Most of these patients are asymptomatic, and will have more than one bone involved. The monostotic presentation of the disease is much less common, but not rare. Monostotic Paget's disease involving the mandible, however, is very unusual. Only six cases have been reported since 1945, and bone imaging did not play a significant role in the diagnoses of these reports. A patient with proven Paget's disease of the mandible presented with a rather dramatic "bearded" image on a bone scan.
Subject(s)
Mandibular Diseases/diagnostic imaging , Osteitis Deformans/diagnostic imaging , Female , Humans , Middle Aged , Radionuclide Imaging , Technetium Tc 99m Medronate/analogs & derivativesABSTRACT
Metastatic neoplasms to the thyroid that become clinically apparent are rare, but a patient that presents with a thyroid nodule and a history of a prior malignancy elsewhere (especially a renal neoplasm) should be thought to have a metastatic nodule first and a nodule of thyroid origin second. This report describes a 66-year-old woman who presented with a large symptomatic thyroid nodule and a history of a right nephrectomy for renal carcinoma two years previously.