Effect of an Intranasal Corticosteroid on Quality of Life and Local Microbiome in Young Children With Chronic Rhinosinusitis: A Randomized Clinical Trial.

Importance: Intranasal corticosteroids (INCs) remain the first-line treatment of chronic rhinosinusitis (CRS) in both adults and children, despite the lack of evidence regarding their efficacy in the pediatric population. Similarly, their effect on the sinonasal microbiome has not been well documented. Objective: To assess the clinical, immunological, and microbiological effects of 12 weeks of an INC in young children with CRS. Design, Setting, and Participants: This open-label randomized clinical trial was performed in a pediatric allergy outpatient clinic in 2017 and 2018. Children aged 4 to 8 years with CRS diagnosed by a specialist were included. Data were analyzed from January 2022 to June 2022. Interventions: Patients were randomized to receive intranasal mometasone in an atomizer for 12 weeks (1 application per nostril, once per day) and supplemental 3-mL sodium chloride (NaCl), 0.9%, solution in a nasal nebulizer once a day for 12 weeks (INC group) or 3-mL NaCl, 0.9%, solution in a nasal nebulizer once a day for 12 weeks (control group). Main Outcomes and Measures: Measures taken both before and after treatment included the Sinus and Nasal Quality of Life Survey (SN-5), a nasopharynx swab for microbiome analysis by next-generation sequencing methods, and nasal mucosa sampling for occurrence of innate lymphoid cells (ILCs). Results: Of the 66 children enrolled, 63 completed the study. The mean (SD) age of the cohort was 6.1 (1.3) years; 38 participants (60.3%) were male and 25 (39.7%) were female. The clinical improvement reflected by reduction in SN-5 score was significantly higher in the INC group compared with the control group (INC group score before and after treatment, 3.6 and 3.1, respectively; control group score before and after treatment, 3.4 and 3.8, respectively; mean between-group difference, -0.58; 95% CI, -1.31 to -0.19; P = .009). The INC group had a greater increase in nasopharyngeal microbiome richness and larger decrease in nasal ILC3 abundance compared with the control group. A significant interaction was observed between change in microbiome richness and the INC intervention on the prediction of significant clinical improvement (odds ratio, 1.09; 95% CI, 1.01-1.19; P = .03). Conclusions and Relevance: This randomized clinical trial demonstrated that treatment with an INC improved the quality of life of children with CRS and had a significant effect on increasing sinonasal biodiversity. Although further investigation is needed of the long-term efficacy and safety of INCs, these data may reinforce the recommendation of using INCs as a first-line treatment of CRS in children. Trial Registration: ClinicalTrials.gov Identifier: NCT03011632.

Upper-airway dysbiosis related to frequent sweets consumption increases the risk of asthma in children with chronic rhinosinusitis.

BACKGROUND: Innate immunity response to local dysbiosis seems to be one of the most important immunologic backgrounds of chronic rhinosinusitis (CRS) and concomitant asthma. We aimed to assess clinical determinants of upper-airway dysbiosis and its effect on nasal inflammatory profile and asthma risk in young children with CRS. METHODS: We recruited one hundred and thirty-three children, aged 4-8 years with doctor-diagnosed CRS with or without asthma. The following procedures were performed in all participants: face-to-face standardized Sinus and Nasal Quality of Life questionnaire, skin prick test, taste perception testing, nasopharynx swab, and sampling of the nasal mucosa. Upper-airway dysbiosis was defined separately by asthma-specific microbiome composition and reduced biodiversity. Multivariate methods were used to define the risk factors for asthma and upper-airway dysbiosis and their specific inflammatory profile of nasal mucosa. RESULTS: The asthma-specific upper-airway microbiome composition reflected by the decreased ratio of Patescibacteria/Actinobacteria independently of atopy increased the risk of asthma (OR:8.32; 95%CI: 2.93-23.6). This asthma-specific microbiome composition was associated with ≥ 7/week sweet consumption (OR:2.64; 95%C:1.11-6.28), reduced biodiversity (OR:3.83; 95%CI:1.65-8.87), the presence of Staphylococcus strains in the nasopharynx (OR:4.25; 95%CI:1.12-16.1), and lower expression of beta-defensin 2, IL-5, and IL-13 in the nasal mucosa. The reduced biodiversity was associated with frequent antibiotic use and with a higher nasal expression of IL-17 and T1R3 (sweet taste receptor). In asthmatic children, reduced sweet taste perception was observed. CONCLUSIONS: Specific upper-airway dysbiosis related to frequent sweet consumption, frequent antibiotic courses, and altered nasal immune function increases the risk of asthma in young children with CRS.

Cervico-ocular reflex upregulation in dizzy patients with asymmetric neck pathology.

OBJECTIVES: The aim of this study was to investigate whether the restriction in neck rotation and increased neck muscle tension could be causally related to vertigo and dizziness. MATERIAL AND METHODS: Seventy-one patients reporting vertigo and/or imbalance were divided into 2 groups: 45 subjects with unilateral restriction (R+) and 26 without restriction (R-) of cervical rotation and muscle tension in the clinical flexion-rotation test. The normal caloric test was the inclusion criterion. The control group comprised 36 healthy volunteers with no history of vertigo. The vestibulo-ocular reflex (VOR) and the cervico-occular reflex (COR) were measured through the videonystagmography (VNG) sinusoidal pendular kinetic test in the conditions of not inactivated head and immobilized head, respectively. The VNG-head torsion test (VNG-HTT) nystagmus was recorded. RESULTS: Among the reported complaints, neck stiffness, headaches and blurred vision were more frequent in the R+ group than in both the R- group and the control group. VNG revealed an increased COR gain and the presence of VNG-HTT nystagmus in the R+ group only. Similarly, only in the R+ group a positive relationship between COR and VOR was observed. CONCLUSIONS: Patients with asymmetric restriction in neck rotation and increased neck muscle tension reveal the tendency to have an increased response of the vestibular system, along with co-existing COR upregulation. Further research is needed to investigate the relationships between the activation of cervical mechanoreceptors and dizziness pathomechanisms. Int J Occup Med Environ Health. 2019;32(5):723-33.

AUDITORY TEMPORAL PROCESSING TESTS - NORMATIVE DATA FOR POLISH-SPEAKING ADULTS.

BACKGROUND: Several subjects exposed to neurotoxins in the workplace need to be assessed for central auditory deficit. Although central auditory processing tests are widely used in other countries, they have not been standardized for the Polish population. The aim of the study has been to evaluate the range of reference values for 3 temporal processing tests: the duration pattern test (DPT), the frequency pattern test (FPT) and the gaps in noise test (GIN). MATERIAL AND METHODS: The study included 76 normal hearing individuals (38 women, 38 men) at the age of 18 to 54 years old (mean ± standard deviation: 39.4±9.1). All study participants had no history of any chronic disease and underwent a standard ENT examination. RESULTS: The reference range for the DPT was established at 55.3% or more of correct answers, while for the FPT it stood at 56.7% or more of correct answers. The mean threshold for both cars in the GIN test was defined as 6 ms. In this study there were no significant associations between the DPT, FPT and GIN results and age or gender. Symmetry between the ears in the case of the DPT, FPT and GIN was found. CONCLUSIONS: Reference ranges obtained in this study for the DPT and FPT in the Polish population are lower than reference ranges previously published for other nations while the GIN test results correspond to those published in the related literature. Further investigations are needed to explain the discrepancies between normative values in Poland and other countries and adapt tests for occupational medicine purposes.

Hearing threshold shift measured by otoacoustic emissions after shooting noise exposure in soldiers using hearing protectors.

OBJECTIVE: The aim of the study was to assess the influence of short-term impulse noise to temporary threshold shift in soldiers using hearing protectors. STUDY DESIGN AND SETTING: The study included 80 subjects with correct tympanic membrane and thresholds measured by pure-tone audiometry less than 20 dB. There were two groups: 40 soldiers protected during shooting and 40 young males who didn't shoot. TEOAE were performed by ILO 292 Echoport Otodynamics device 3 to 5 minutes before shooting and 2 minutes and 1, 2, and 3 hours after shooting. RESULTS: Short-term exposure to impulse noise generated by five gunshots from the rifle kbk AKMS hasn't induced temporary threshold shift of hearing for chosen frequencies in soldiers using hearing protectors. Spectral analysis for chosen frequencies revealed that measurement reproducibility, stimuli level, and probe stability appeared to be comparable and repeatable. CONCLUSION: Our results show that the use of hearing protectors safeguarded against impulse noise. SIGNIFICANCE: The use of earmuffs is strongly recommended because they seem to sufficiently attenuate shooting noise.

The association between positional vertebral and basilar artery flow lesion and prevalence of vertigo in patients with cervical spondylosis.

OBJECTIVE: The aim of our investigations was to characterize the relationship between basilar artery (BA) flow velocity in patients with cervical spondylosis and age, grade of radiological changes, prevalence of vertigo, and decreased blood flow velocity through vertebral arteries (VA) after head rotation. STUDY DESIGN AND SETTING: A prospective, controlled study in academic neurotology was conducted. We examined 80 patients with radiological evidence of cervical spondylosis. Forty patients complained of positional vertigo lasting more than 6 months. Patients were examined by transcranial Doppler ultrasound with head rotations. RESULTS: We showed significant association between velocity flow in basilar artery after neck rotation and age, prevalence of vertigo, grade of radiological changes, and positional vertebral artery flow lesion. CONCLUSION: Spondylotic-induced VA compression may be the reason for decreased blood flow velocity in the basilar artery during head rotation, which may be of particular importance for older patients, especially complaining of vertigo. SIGNIFICANCE: Every patient with high-grade cervical spondylosis, especially complaining of vertigo, should be examined by using TCD (transcranial Doppler) with head rotations. In case of positional blood flow reduction during head rotation, further angiographic examination should be undertaken so as to plan suitable treatment. EBM RATING: C-4.

[Analysis of select diagnostic examination results and their connection with cervical vertigo diagnosis]. / Analiza wyników wybranych badan diagnostycznych i ich zwiazek z rozpoznawaniem zawrotów glowy pochodzenia szyjnego.

Cervical spine spondylotic changes are known to cause vertigo, but diagnostic criteria are not established. The aim of present study was to asses diagnostic value of classical radiologic, electronystagmographic (ENG) and transcranial doppler ultrasonographic (TCD) examinations in cervical vertigo diagnostic process. Forty patients with vertigo lasting more than 6 months with radiological evidence of cervical spondylosis participated in the study. All patients had ENG and TCD with neck rotation test. Cervical vertigo was diagnosed in patients with positive neck rotation test in: TCD examination (15% decrease of vertebral artery flow) and/or ENG examination (cervical nystagmus). We diagnosed cervical vertigo in 65% of patients. Multivariate model of logistic regression analysis showed that the best statistical predictors of cervical vertigo were: asymmetry of vertebral artery flow in TCD (OR: 92.2; 95% CI: 6,2-1381) and presence of osteophytes and discopathy in radiological examination (OR: 64.1; 95% CI: 1,3-1107). The transcranial doppler ultrasonographic examinations seems to be useful for diagnosing cervical vertigo.

[Kinesitherapy treatment for patients with cervical vertigo (preliminary report)]. / Wczesna ocena leczenia chorych z zawrotami glowy pochodzenia szyjnego za pomoca kinezyterapii (doniesienie wstepne).

The aim of the study was to assess the effectiveness of kinesitherapy in cervical vertigo treatment. Thirty two patients with cervical vertigo, aged 20 to 75, were examined. The patients performed kinesitherapy exercises three times a day for four weeks. The effects of therapy were assessed by everyday task self-control cards and ENG examinations. In the present study, objective improvement was noticed in 18,7% patients. Subjective improvement assessed by the patients everyday task self-control cards reached 62.5%. Kinesitherapy seems to be the good method of treating neck-related vertigo, but it should be confirmed in further study on the large group of patients.

[Influence of positional blood flow disorders in the vertebral and basilar arteries on the prevalence of vertigo in patients with cervical spondylosis]. / Ocena wplywu czynnosciowych zaburzen predkosci przeplywu w tetnicach kregowych i w tetnicy podstawnej na wystepowanie zawrotów glowy u pacjentów ze zmianami zwyrodnieniowymi odcinka szyjnego kregoslupa.

The aim of present study was to characterize the association between prevalence of vertigo and age, grade of radiological changes and positional vertebral (VA) and basilar artery (BA) flow lesion in patients with cervical spondylosis. We examined 80 patients with radiological evidence of cervical spondylosis. Forty patients complained positional vertigo. Patients with neurological symptoms were excluded from the study. ENG was used to exclude patients with another causes of vertigo. TCD evaluations of the distal part of VA and the proximal part of BA were performed with a 2-MHz probe via the suboccipital window. We showed significant and independent association between prevalence of vertigo and age, grade of radiological changes, and positional vertebral and basilar artery flow lesion velocity in multivariate logistic regression model. Spondylotic-induced VA compression may be the reason of decreased blood flow velocity in the basilar artery during head rotation, causing positional vertigo.

Temporary hearing threshold shift measured by otoacoustic emissions in subjects exposed to short-term impulse noise.

OBJECTIVES: The aim of the study was to assess the influence of short-term impulse noise on the size and dynamics of temporary threshold shift, which precedes permanent threshold shift, i.e. noise-induced hearing loss. It was hoped to use the findings for preventive activities. MATERIALS AND METHODS: The study included 80 healthy subjects (160 ears), aged 19-23 years, divided into two groups: group I comprised 40 recruit soldiers put to the shooting training, and group II consisted of 40 young male controls. All subjects had to show normal hearing with pure tone audiometric thresholds between 10-15 dB. Transient evoked otoacoustic emission (TOAE) measurements were performed by ILO 292 Echoport Otodynamics device 3-5 min before shooting and then 2 min, 1, 2 and 3 h, respectively after shooting. In group II the time intervals were similar. RESULTS: It was found that the gunshot impulse noise from the kbk AKMS rifle caused temporary hearing threshold shift (TTS) at 1, 2, 3, 4 and 5 kHz frequencies of 1.07, 0.96, 1.41, 0.88 and 1.25 dB SPL, respectively. TIS turned out to be maximum at 4 and 5 kHz and minimum at 1 and 2 kHz. CONCLUSIONS: Short-term impulse noise generated by the rifle gunshots induces rather small temporary threshold shift of hearing. Anyhow, considering possibilities of different weapon noises in the military environment as well as various sources of industrial impulse noise, the usage of hearing protectors should be highly recommended.