Increasing the efficiency of dental disease prevention in children of younger school age through participation in a stomatological performance.

OBJECTIVE: Aim: To study the awareness of children and their parents in the prevention of dental diseases and to propose educational measures to increase it. PATIENTS AND METHODS: Materials and Methods: To achieve the goal of the study, a survey of 628 children, aged 7-11 years, younger schoolchildren of Poltava and 479 of their parents was conducted using the previously developed "Dental Questionnaire for Children and Parents". The questionnaire was based on the main indicators of the dental health of children and adolescents in accordance with the EGOHID II project (2008) and the WHO dental questionnaire (2013), and it took into account the proposals of dental organizations that regulate the assessment of oral health in children. Based on the results of the survey, a script for oral hygiene lesson for junior schoolchildren was developed using theatrical and game elements. RESULTS: Results: The results of the conducted research indicate the low sanitary and hygienic awareness of parents, the paternalism of their thinking, which leads to the lack of control over the hygiene of the oral cavity of children. In order to solve this problem, a scenario of informational explanatory conversation was created with the involvement of modern methods of communication and intern doctors who have acquired relevant knowledge and communication skills. CONCLUSION: Conclusions: Informing of children about the prevention of dental diseases in the form of a dental performance is a relevant sanitary and educational event that motivates children to observe the rules of oral hygiene.

FUNCTIONING OF NO-CYCLE IN THE ORAL FLUID IN CHILDREN OF PRIMARY SCHOOL AGE WITH TYPE 1 DIABETES MELLITUS IN THE TREATMENT OF CHRONIC CATARRHAL GINGIVITIS.

OBJECTIVE: The aim: To determine the activity of NO-synthase and arginase in oral fluid in children with type 1 diabetes mellitus and to evaluate the efficacy of the treatment scheme we elaborated in the treatment of chronic catarrhal gingivitis. PATIENTS AND METHODS: Materials and methods: 82 children were examined, they were divided into groups by presence of gingivitis and diabetes mellitus. NO-synthase (NOS) activity was determined in oral fluid by the difference in nitrite concentration before and after incubation. The arginase activity was determined in oral fluid by the difference in the concentration of L-ornithine before and after incubation. RESULTS: Results: Use our treatment scheme in children with chronic catarrhal gingivitis and type 1 diabetes mellitus lead to a change in the polarization of oral macrophages towards the predominance of M2 polarization in 1 month. The polarization of macrophages changed to the predominance of M1 polarization activity in 1 year. CONCLUSION: Conclusions: We have elaborated a scheme for the treatment of chronic catarrhal gingivitis in children with type 1 diabetes mellitus. It normalizes the polarization of oral macrophages caused by exposure to chronic catarrhal gingivitis as a local pathogenetic factor.

THE LEVEL OF INTERLEUKIN-18 IN THE ORAL FLUID IN PRIMARY SCHOOL CHILDREN WITH CHRONIC CATARRHAL GINGIVITIS AND TYPE I DIABETES MELLITUS.

OBJECTIVE: The aim: Of our research work was to study the level of proinflammatory interleukin-18 (IL-18) in the oral fluid of children with type I diabetes mellitus (DM), and to determine their periodontal status and the level of oral hygiene. PATIENTS AND METHODS: Materials and methods: 82 children were examined, they were divided into groups by presence of gingivitis and diabetes mellitus. The level of interleukin-18 in oral fluid was determined by immunoassay. RESULTS: Results: In patients with chronic catarrhal gingivitis and type I diabetes mellitus the level of interleukin-18 in oral fluid is the highest (70.91±7.48 pg / ml); the level of interleukin-18 in children with diabetes mellitus and healthy gums is high enough too, it is 14.87±1.11 pg / ml. Interleukin-18 is 3.41±0.25 pg / ml in healthy children with healthy gums. It is 5.74±0.27 pg / ml in somatically healthy children with chronic catarrhal gingivitis. CONCLUSION: Conclusions: We indicated that an increase in the value of interleukin-18 in oral fluid is associated with the presence of diabetes mellitus in children. Moreover, this cytokine can be considered as a potential biomarker of gum inflammation in children with diabetes mellitus.

INFLUENCE OF MICROBIOTA ON THE CLINICAL AND IMMUNO-HISTOCHEMICAL CHARACTERISTICS OF CHRONIC GENERALIZED CATARRHAL GINGIVITIS IN CHILDREN.

OBJECTIVE: The aim: The purpose of the study is to characterize the influence of quantitative and qualitative composition of gingival microbiota on the status of the main immune system cells, localized in the gums, in chronic generalized catarrhal gingivitis in children. PATIENTS AND METHODS: Materials and methods: The study involved 26 children aged 9 to 16 years, patients with chronic generalized catarrhal gingivitis mild to moderate severity (CGCG) and 18 children with intact gums were comparison group. We determined the hygienic indices Fedorov, has been received, Silness-Loe, PMA, bleeding index for Myuleman and intensity of caries index CFD + cf, CFD. Histological and immunohistochemical studies were performed on serial sections kriostatnyh who made biopsy of gingival papillae. Microbiological study gingival part of crown plaque was performed by multiplexed PCR in real time. RESULTS: Results: Value hygienic indices in children with CGCG higher than in healthy, indicating the difficulty of care in the presence of periodontal inflammation. As a result of immunohistochemical studies revealed that HLA-DR + cells under conditions of active disease migrate to mucosal lamina propria epithelium. Number of CD3 + cells in the epithelium CGCG was significantly higher than the number in the intact epithelium and was the most numerous of population. In the biopsy of affected children significantly reduced the number of CD4 + cells. When CGCG quantitative total bacterial mass, Lactobacillus spp., Enterobacteriaceae, Gardnerella vaginalis / Prevotella bivia / Porphyromonas spp. in the sample CROWN dental plaque was significantly higher than rates under physiological conditions, and may serve as diagnostic criteria of dysbiosis. CONCLUSION: Conclusions: So, CGCG is a disease in the etiology of which is one of the leading roles played by microbial factor, namely, the value of the quantitative ratios of certain types of microorganisms of dental plaque compared to the total bacterial mass of plaque. Therefore, it is reasonable to include comprehensive treatment CGCG drugs in children, leading to natural immunostimulation which causes restoration of local immunity in the gum tissue and drugs to restore quantitative and qualitative composition of normal microflora of the child, thus providing a high therapeutic effect and serve as justification their choice.