DNA Polymorphisms and mRNA Levels of Immune Biomarkers as Candidates for Inflammatory Postpartum Disorders Susceptibility in Italian Buffaloes.

The immunological genes that may interact with inflammatory postpartum diseases in Italian buffaloes were examined in this study. A total number of 120 female Italian buffaloes (60 normal and 60 with inflammatory reproductive diseases) were employed. Each buffalo's jugular vein was pierced to get five milliliters of blood. To obtain whole blood and extract DNA and RNA, the blood was placed within tubes containing sodium fluoride or EDTA anticoagulants. The immunological (IKBKG, LGALS, IL1B, CCL2, RANTES, MASP2, HMGB1, and S-LZ) genes' nucleotide sequence differences between healthy buffaloes and buffaloes affected by inflammatory reproductive diseases were found by employing PCR-DNA sequencing. According to Fisher's exact test (p ˂ 0.01), there were noticeably different probabilities of all major nucleotide changes spreading among buffalo groups with and without reproductive problems. Buffaloes were significantly more likely to express the examined genes when they had inflammatory reproductive diseases. The outcomes might support the significance of these markers' nucleotide variations and gene expression patterns as indicators of the prevalence of inflammatory reproductive disorders and provide a workable buffalo management policy.

Performance, Blood Lipid Profile, and the Expression of Growth Hormone Receptor (GHR) and Insulin-like Growth Factor-1 (IGF-1) Genes in Purebred and Crossbred Quail Lines.

The aim was to evaluate the performance, blood lipid profile, and the relative expression of growth-related genes in purebred white and brown quail lines and their crossbred lines. A total of 240 one-day-old Japanese quail chicks of white and brown line, their crossbred line (WBQ: male white × female brown), and reciprocal crossbred line (BWQ: male brown × female white) were divided into four equal groups (60 birds each). The white quail line showed significantly higher final body weight, daily gain, and feed intake compared with the other quail lines (p < 0.001). Meanwhile, both crossbred quail lines (WBQ and BWQ) showed significantly lower FCR compared with both purebred quail lines (p = 0.001). Both crossbred quail lines showed greater dressing percentages compared with both purebred quail lines (p = 0.038). The brown quail line showed significantly (p = 0.05) higher levels of serum triglycerides and VLDL compared with the white and BWQ lines. The WBQ crossbred line exhibited significantly higher mRNA expression of GHR and IGF-1 genes compared with other quail lines (p < 0.001). Both crossbred lines (WBQ and BWQ) exhibited negative heterosis percentages for body weight (−4.39 and −3.90%, respectively) and feed intake (−10.87 and −14.59%, respectively). Meanwhile, heterosis percentages for FCR (−6.46 and −9.25%, respectively) and dressing percentage (7.54 and 6.38%, respectively) were improved in both crossbred lines. The WBQ line showed high heterosis percentages for the expression of GHR and IGF-1 genes (52.28 and 88.81%, respectively). In conclusion, the WBQ line exhibited significantly greater dressing percentage and better FCR, as well as higher mRNA expression of GHR and IGF-1 genes. These results may be helpful to improve breeding programs and to develop commercial lines of meat-type Japanese quail.

Response of two strains of growing Japanese quail (Coturnix Coturnix Japonica) to diet containing pomegranate peel powder.

Four hundred and eighty 1-day-old Japanese quail chicks from white and brown strains, 240 birds from each strain, were divided into 4 treatment groups with 6 replicates each (10 birds/replicate). Both strains of quail were fed diets containing 0%, 3%, 6%, and 9% pomegranate peel (PP) powder for a period of 42 days. Results revealed that the final body weight (BW), total feed intake (TFI), body weight gain (BWG), gizzard percentage, and eviscerated carcass weight of white growing quails were significantly (P < 0.05) higher than the brown strain. Quail group fed with 6% PP powder had the highest (P < 0.001) average final BW and BWG (P < 0.001). The dietary PP powder at different levels significantly (P = 0.032) decreased TFI, but had no significant effect on carcass traits except for the liver percentage. Quails fed diet 3% and 9% PP powder had significant (P < 0.001) increased count of immune cells, and improved antioxidant potency. White quails fed diet 3% and 6% PP powder had greater expression of hepatic GHR gene. The expression of hepatic IGF-1 gene was significantly (P < 0.05) higher for brown quails fed diet 6% and 9% PP powder. White quails recorded the highest value of total return (TR). Quails fed diet 6% and 9% PP powder significantly (P < 0.001) recorded higher net return (NR) values. In conclusion, dietary inclusion of PP powder at levels up to 9% for Japanese quail strains improved the growth performance, increased antioxidant properties, enhanced hepatic gene expression, and did not show any adverse influence on carcass quality and blood indices of Japanese quail. Moreover, it increased the NR.

Effect of dietary pomegranate peel powder on productive traits, blood chemistry, economic efficiency and the expression of FSHR and LH-ß genes in two strains of laying Japanese quail.

The current experiment was designed to study the response of two strains of laying Japanese quail to diet containing different levels (0%, 3%, 6%, and 9%) of pomegranate peel (PP) powder for a period of 6 weeks, on egg production, blood chemistry, carcass traits, expression of FSHR and LH-ß genes, and economic efficiency. A total of 576 6-week-old Japanese quails were made up of two strains: white quails (n=288) and brown quails (n=288). Four treatment groups were randomly distributed for each strain. Each group was subdivided into 6 replicates of 10 birds each. Results revealed that the white strain showed significant (P<0.05) higher values in final body weight (BW), egg quality parameters, weights of dressed carcass, and total return (TR) compared with the brown strain. Brown strain had higher hen day egg production % (HDEP%). A significant (P<0.05) interaction effects between genetic type and the dietary PP powder levels was recorded in growth traits, some egg production traits, and mRNA expression of FSHR and LH-ß genes. Results concluded that all the dietary levels of PP powder up to 9% improved growth traits, egg production traits, fertility% (P=0.001), and hatchability% (P=0.007). Moreover, they have a safely biochemical effect on the level of urea (P=0.002) and the concentration of aspartate amino transferase (AST) (P<0.001). It also enhanced mRNA expression of FSHR and LH-ß genes, but showed non-significant (P>0.05) influence on carcass traits. Economically, the net return (NR) was increased in quails fed on 6% and 9% PP powder diets.