Modulation of innate immune responses in the flame scallop Ctenoides scaber (Born, 1778) caused by exposure to used automobile crankcase oils.

The used automobile crankcase oils are potential sources of contaminant elements for the coastal-marine ecosystems, affecting mainly the immunological system of organisms that feed by filtration, e. g. scallops. This study examined the effects of a water-soluble fraction of used automobile crankcase oils (WSF-UACO) on innate cellular- and humoral immune responses of the flame scallop Ctenoides scaber. The scallops were exposed to ascending concentrations of 0, 0.001, 0.01, and 0.1 of WSF-UACO under a static system of aquaria during 7 and 13 d. The viability, haemocyte total count (HTC), lysosomal membrane destabilization (LMD), phagocytosis, and protein concentration in hemolymph samples withdrawn taken from the blood sinus as well as lysozyme activity of the digestive gland were measured as immune endpoints. A decrease in cellular immune competence in scallops exposed to WSF-UACO was observed, with significant impairment of viability, HTC, and phagocytosis. LMD index increased about exposure concentrations, and plasma protein concentrations augmented to 0.01 and 0.1% during 13 d. Lysozyme activity increased in scallops exposed to WSF-UVCO during 7 d, to level off in the chronic period. Lysozyme activity and enhanced plasma proteins could act as compensatory responses when cell parameters tend to fall, helping to the regulation of microbial microflora and possible invasion of pathogenic microbes as well as defense against xenobiotics. The results demonstrate that the immunological responses of C. scaber are highly sensitive to the complex chemical mixture of contaminants, and it could be used for evaluating biological risks of hazardous xenobiotics in tropical marine environments. Republic of Ecuador.

Colorless spherule cells and lysozyme contribute to innate immunological responses in the sea urchin Lytechinus variegatus, exposed to bacterial challenge.

The sea urchin Lytechinus variegatus is considered a good candidate for aquaculture, but bacterial diseases are a major challenge in culture conditions. The innate immunological defenses of L. variegatus to bacterial challenges were assessed through hematology parameters, in vitro phagocytosis, lysozyme activity and total plasma protein concentrations in cell-free coelomic fluid. Adult sea urchins were inoculated with Microccocus lysodeikticus, Escherichia coli and Vibrio parahaemolyticus in the cavity coelomic. Filtrated and sterile seawater (FSW) injected and non-injected sea urchins were used as control groups. Righting time, external aspects and behavior of sea urchins were evaluated. Twenty-four hours post-inoculation, we found an increase in the population of colorless spherule cells (CLS), phagocytosis, and humoral responses in sea urchins challenged by bacterial inoculations. Righting time was not affected by the treatments and apparent external signs of disease were not observed at least during 96h post-inoculation. The immunological system of L. variegatus quickly eliminated pathogenic microorganisms. CLS and lysozyme activity cooperate in the immune defenses of L. variegatus, showing an extraordinary efficiency for adjusting the immune defenses under stress caused by microbes. We recommend that the cellular and humoral markers serve as routine tests to monitor health status in sea urchins.