Marine diatom algae cultivation in simulated dairy wastewater and biomass valorization.

Liquid byproducts and organic wastes generated from dairy processing units contribute as the largest source of industrial food wastewater. Though bacteria-mediated treatment strategies are largely implemented, a more effective and innovative management system is needed of the hour. Thus, the current study involves the cultivation of centric diatoms, Chaetoceros gracilis, and Thalassiosira weissflogii in simulated dairy wastewater (SDWW) formulated using varying amounts of milk powder with artificial seawater f/2 media (ASW). The results revealed that cell density and biomass productivity were highest in the 2.5% SDWW treatment cultures of both the strains, the maximum being in C. gracilis (7.5 × 106 cells mL - 1; 21.1 mg L-1 day-1). Conversely, the total carotenoid, chrysolaminarin, and phenol content were negatively impacted by SDWW. However, a considerable enhancement in the total lipid content was reported in the 2.5% SDWW culture of both species. Furthermore, the fatty acid profiling revealed that though the total polyunsaturated fatty acid (PUFA) content was highest in the control setups, the total mono polyunsaturated fatty acid (MUFA) content was higher in the 5% SDWW setups (30.66% in C. gracilis and 33.21% in T. weissflogii). In addition to it, in the cultures utilizing energy from external carbon sources provided by SDWW, the biodiesel produced was also enhanced owing to the heightened cetane number. Thus, the current study evidently highlights the organic carbon acquisition potential of marine diatoms with the scope of providing sustainable biorefinery.

Therapeutic Potential of Seaweed-Derived Laminaran: Attenuation of Clinical Drug Cytotoxicity and Reactive Oxygen Species Scavenging.

ß-glucan has been shown to be effective for several diseases such as immune regulation and blood pressure suppression. Seaweed contains a ß-1,3/1,6-glucan called laminaran. The present commercial source of ß-glucan is black yeast; however, a fermentation process using organic carbon substrates makes production unsustainable, whereas macroalgae provide a sustainable alternative with the use of CO2 and seawater as growth substrates. However, bioactivity studies on laminaran are limited. We aimed to evaluate whether laminaran can scavenge reactive oxygen species (ROS) and attenuate cytotoxicity caused by clinical drugs such as indomethacin (Ind) and dabigatran (Dab). Electron spin resonance assay revealed that laminaran scavenged singlet oxygen (1O2) and superoxide anions (O2•-) directly but did not scavenge hydroxyl radicals (•OH). Mitochondrial ROS detection dye showed that laminaran scavenged mitochondrial O2•- produced upon administration of Ind or Dab. Moreover, significant reductions in •OH and peroxynitrate (ONOO-) levels were observed. Since •OH and ONOO- are generated from O2•- in the cells, laminaran could indirectly suppress the generation of •OH and ONOO- via the removal of O2•-. Both Ind and Dab induce cell injury via ROS production. Laminaran attenuated the cytotoxicity derived from these drugs and may represent a functional food with anti-aging and disease prevention properties.

Treating agricultural non-point source pollutants using periphyton biofilms and biomass volarization.

Untreated domestic wastewater and agricultural runoff are emerging as a potent cause of non-point source (NPS) pollutants which are a major threat to aquatic ecosystems. Periphyton biofilm-based technologies due to their high growth rate, energy efficiency and low input costs offer promising solutions for controlling nutrient pollution in agricultural systems. In this study we employed periphyton floway to treat NPS pollution from the agricultural watershed. The process performance of outdoor single pass algae floway (AFW) was evaluated. Steady state average biomass concentration of 11.73 g m-2 d-1 and removal rate of nitrogen: 0.60 g m-2 d-1, phosphorus: 0.27 g m-2d-1, arsenic: 9.26 mg m-2 d-1, chromium: 255.3 mg m-2 d-1 and lead: 238.6 mg m-2 d-1 was achieved. In addition, the microalgae and their associated bacterial diversity and dynamics were analyzed. The results revealed a high diversity and rapid variations in the microbiome structure with diatom and cyanobacteria dominance combined with high N fixing and P solubilizing bacteria during most of the operational period. Elemental analysis of periphyton biomass was done for its safe use as slow-release fertilizer. Biofuel feedstock potential and nanoparticle generation potential of the biomass were analyzed. This work highlights the potential use of periphyton biofilms in remediation and recycling of NPS pollutants with simultaneous resource recovery.

Deciphering functional biomolecule potential of marine diatoms through complex network approach.

Marine diatoms are unique reservoirs of bioactive compounds having enormous applications in therapeutics. But high-throughput screening methods are needed to elucidate the interaction between numerous biomolecules and their targets, facilitating rapid screening for novel drug molecules. So, in the present study chemical constituents were extracted from five marine diatoms using un-targeted metabolite profiling and in-silico virtual screening bioinformatics was employed to predict their bioactivity and molecular targets. A total of 17 chemical constituents out of 51 showed interactions with 76 protein targets associated with 213 pathways. Ingredient-target-pathway network revealed oleic acid, linoleic acid and cholest-5-en-3-ol as major active constituents. Core subnetwork and protein association network showed involvement of these compounds in key metabolic pathways related to cell signaling, cell growth and metabolism of xenobiotics. Thus, the present study for the first time revealed the main active ingredients and their associated pathways from marine diatoms using complex network approach.

Impact of organic carbon acquisition on growth and functional biomolecule production in diatoms.

Diatoms are unicellular photosynthetic protists which constitute one of the most successful microalgae contributing enormously to global primary productivity and nutrient cycles in marine and freshwater habitats. Though they possess the ability to biosynthesize high value compounds like eicosatetraenoic acid (EPA), fucoxanthin (Fx) and chrysolaminarin (Chrl) the major bottle neck in commercialization is their inability to attain high density growth. However, their unique potential of acquiring diverse carbon sources via varied mechanisms enables them to adapt and grow under phototrophic, mixotrophic as well as heterotrophic modes. Growth on organic carbon substrates promotes higher biomass, lipid, and carbohydrate productivity, which further triggers the yield of various biomolecules. Since, the current mass culture practices primarily employ open pond and tubular photobioreactors for phototrophic growth, they become cost intensive and economically non-viable. Therefore, in this review we attempt to explore and compare the mechanisms involved in organic carbon acquisition in diatoms and its implications on mixotrophic and heterotrophic growth and biomolecule production and validate how these strategies could pave a way for future exploration and establishment of sustainable diatom biorefineries for novel biomolecules.

Indoor mass cultivation of marine diatoms for biodiesel production using induction plasma synthesized nanosilica.

In this work, two benthic marine diatoms Chaetoceros sp. and Thalassiosira sp. were grown in modified f/2 medium in which normal silica was replaced with inductively coupled plasma (ICP) nanosilica for indoor mass cultivation and its impact on growth, lipid content, lipid quality and metabolite production were monitored. Results indicate thatunder mass cultivation using ICP nano silica medium, Thalassiosirasp. reached 3.6 and Chaetoceros sp. reached 3.2-fold higher cell density compared to normal Si medium. The primary metabolite production and total lipid content was higher in Chaetoceros sp. (44.33 ± 2.51% DCW) compared to Thalassiosira sp. (29.66 ± 1.52% DCW). In mass cultivation, ICP synthesized nanosilica powder was effective in enhancing the cell density, production of metabolites, pigments, and lipids in the marine diatoms studied. This is the first report on the use of ICP nanosilica in carrying out indoor mass cultivation of marine diatom isolates as potential biodiesel and biomolecule feedstocks.

New paradigm in diatom omics and genetic manipulation.

Diatoms are one of the most heterogeneous eukaryotic plankton known for regulating earth's biogeochemical cycles and maintaining the marine ecosystems ever since the late Eocene epoch. The advent of multidisciplinary omics approach has both epitomized and revolutionized the nature of their chimeric genetic toolkit, ecophysiology, and metabolic adaptability as well as their interaction with other communities. In addition, advanced functional annotation of transcriptomic and proteomic data using cutting edge bioinformatics tools together with high-resolution genome-scale mathematical modeling has effectively proven as the catapult in solving genetic bottlenecks in microbial as well as diatom exploration. In this review, a corroborative summation of the robust work done in manipulating, engineering, and sequencing of the diatom genomes besides underpinning the holistic application of omics in transcription and translation has been discussed in order to shrewd their multifarious novel potential in the field of biotechnology and provide an insight into their dynamic evolutionary relevance.

Wealth from waste: Diatoms as tools for phycoremediation of wastewater and for obtaining value from the biomass.

Diatoms are a type of microalgae with diverse capabilities which make them useful for multiple applications. The abundance of diatoms in water bodies facilitates the removal of pollutants from wastewater originating from different industries, such as agriculture and other anthropogenic sources. The unique photosynthetic, cellular and metabolic characteristics of diatoms allows them to utilize pollutants like nitrate, iron, phosphate, molybdenum, silica, and heavy metals, such as copper, cadmium, chromium, lead, etc., which make diatoms a good option for wastewater treatment. In addition, the biomass produced by diatoms growth on wastewaters has diverse applications and can, therefore, be valuable. This review focusses on the unique capabilities of diatoms for wastewater remediation and the capture of carbon dioxide, concomitant with the generation of valuable products. Diatom biorefinery can be a sustainable solution to wastewater management, and the biomass obtained from treatment can be turned into biofuels, biofertilizers, nutritional supplements for animal production, and used for pharmaceutical applications containing bioactive compounds like EPA, DHA and pigments such as fucoxanthin.

Marine diatom Thalassiosira weissflogii based biorefinery for co-production of eicosapentaenoic acid and fucoxanthin.

Diatom algae can produce bioactive compounds like fucoxanthin (FX) and ecosapentaenoic acid (EPA) which are of high demand in pharmaceutical and nutraceutical industries. Here, the influence of different light regimes in combination with major nutrients on growth, FX and EPA production by marine diatom Thalassiosira weissflogii were investigated. Batch cultures of T. weissflogii were illuminated under blue (BL), red (RL) and white (WL) light at two intensities. BL regime resulted in higher cell density with a specific growth rate of 2.49µ. Lipid productivity and lipid % as dry cell weight (DCW) was considerably higher in BL with EPA productivity of 33.4 mg L-1d-1. Fucoxanthin content as % DCW reached 0.95 (BL), 0.75 (RL) and 0.81 (WL) at mid exponential growth phase. The results further prove the plasticity of diatoms and provide a way for future metabolic engineering of T. weissflogii for potential microalgal bio-refinery for combined EPA and FX production.

Biodiesel production through algal cultivation in urban wastewater using algal floway.

The aim of this work was to study algal floway (AFW) to treat urban wastewater and to evaluate biomass productivity, lipid contents and biodiesel production. The results indicated the seasonal average algae productivity of 34.83 g dry weight m2 d-1 with a nutrient removal rate of 2.52 g m2 d-1N and 1.25 g m2 d-1P while the lipid content ranged between 14 and 22% of dry cell weight with the highest lipid productivity of 9.29 g m-2 d-1 during summer. Biodiesel quality was superior during summer with high centane number and cold filter plugging point values. High eicosapentaenoic acid content was found during winter growth cycles. AFW algae community was dominated by pennate diatoms during all growing seasons. This study is one of its kinds in Indian wastewaters and it provides fundamental information for further optimization and use of AFW to treat domestic wastewater and to produce algae biofuel feedstock.

The application of ceramsite ecological floating bed in aquaculture: its effects on water quality, phytoplankton, bacteria and fish production.

In recent years, biological floating bed technology has been applied increasingly in aquaculture ponds. In this study we developed a novel floating bed made from ceramsite and studied its effect on water quality, phytoplankton, bacteria and fish growth. Water quality was effectively regulated and controlled in ceramsite floating bed (CFB) ponds with an average transparency of 23.18 cm, ammonia nitrogen (NH4+-N) of 2.30 mg L-1, total nitrogen (TN) of 5.09 mg L-1 and total phosphate (TP) of 1.32 mg L-1 which are lower than in control ponds without CFB. Increased phytoplankton species diversity, bacterial number, metabolic activity and microbial diversity was observed with CFB. At the end of growth stage, feed conversion ratio (FCR) was reduced with a total fish yield of 14,838 kg ha-1 at a survival rate of 77.2% in CFB ponds, which is significantly higher than control (P < 0.05). These results emphasize the potential of ecological floating bed to improve water quality, microalgal diversity, reduce the risk of harmful algal blooms and increase the number, activity and diversity of microorganisms as well as fish yield.

Potential of diatom consortium developed by nutrient enrichment for biodiesel production and simultaneous nutrient removal from waste water.

Because of the decreasing fossil fuel supply and increasing greenhouse gas (GHG) emissions, microalgae have been identified as a viable and sustainable feedstock for biofuel production. The major effect of the release of wastewater rich in organic compounds has led to the eutrophication of freshwater ecosystems. A combined approach of freshwater diatom cultivation with urban sewage water treatment is a promising solution for nutrient removal and biofuel production. In this study, urban wastewater from eutrophic Hussain Sagar Lake was used to cultivate a diatom algae consortium, and the effects of silica and trace metal enrichment on growth, nutrient removal, and lipid production were evaluated. The nano-silica-based micronutrient mixture Nualgi containing Si, Fe, and metal ions was used to optimize diatom growth. Respectively, N and P reductions of 95.1% and 88.9%, COD and BOD reductions of 91% and 51% with a biomass yield of 122.5 mg L-1 day-1 and lipid productivity of 37 mg L-1 day-1 were observed for cultures grown in waste water using Nualgi. Fatty acid profiles revealed 13 different fatty acids with slight differences in their percentage of dry cell weight (DCW) depending on enrichment level. These results demonstrate the potential of diatom algae grown in wastewater to produce feedstock for renewable biodiesel production. Enhanced carbon and excess nutrient utilization makes diatoms ideal candidates for co-processes such as CO2 sequestration, biodiesel production, and wastewater phycoremediation.

A novel growth method for diatom algae in aquaculture waste water for natural food development and nutrient removal.

Diatom algae are known to play an important role as primary producers in many diverse ecosystems, including artificial aquaculture ponds where they also aid in maintaining water quality by consuming excess nutrients. But factors influencing their growth are still poorly understood. In the present study the effect of micronutrients, N:P ratio and silica concentration on benthic diatom Synedra sp. grown in fish pond waste water was studied along with nutrient removal efficiency. We have studied nine different treatments, of which addition of micronutrient mixture Nualgi along with adjusted N:P to 6:1 resulted in highest cell density, followed by silicate enrichment, whereas only N:P adjustment and Nualgi addition had no significant effect on diatom growth. At the end of the growth experiment, the N removal efficiencies of treatment groups (50.23%-65.44%) were significantly higher (P < 0.05) than that of the control group (43.56%), whereas phosphate removal efficiency was significantly higher (P < 0.05) with Nualgi and N:P adjustment (53.37%-68.98%). The silicate consumption was significantly higher in the control group, at 63.87%, than in other experimental groups. These results will give us a new insight into important factors influencing beneficial algae growth and simultaneous nutrient removal from aquaculture waste water.