1.
Res Vet Sci
; 43(3): 407-9, 1987 Nov.
Article
in English
| MEDLINE
| ID: mdl-3444989
ABSTRACT
An efficient and reproducible technique is described for the isolation of transformed sheep pulmonary adenomatosis cells. It includes three basic steps: prolonged trypsinisation to kill fibroblasts, magnetic removal of macrophages and adherence to remove the rapidly adherent cells. The resultant preparations of lung cells were enriched to 96.6 per cent type 2 pneumocytes.