Correction to: Stem Cells from Human Exfoliated Deciduous Teeth Modulate Early Astrocyte Response after Spinal Cord Contusion.

The authors hereby declare that the Figure 4 in page eight of the paper "Stem cells from human exfoliated deciduous teeth modulate early astrocyte response after spinal cord contusion" authored by Fabrício Nicola and colleagues (DOI: 10.1007/s12035-018-1127-4) was mistakenly included.

Stem Cells from Human Exfoliated Deciduous Teeth Modulate Early Astrocyte Response after Spinal Cord Contusion.

The transplantation of stem cells from human exfoliated deciduous teeth (SHED) has been studied as a possible treatment strategy for spinal cord injuries (SCIs) due to its potential for promoting tissue protection and functional recovery. The aim of the present study was to investigate the effects of the early transplantation of SHED on glial scar formation and astrocytic reaction after an experimental model of SCI. Wistar rats were spinalized using the NYU Impactor. Animals were randomly distributed into three groups: control (naive) (animal with no manipulation); SCI (receiving laminectomy followed by SCI and treated with vehicle), and SHED (SCI rat treated with intraspinal SHED transplantation, 1 h after SCI). In vitro investigation demonstrated that SHED were able to express mesenchymal stem cells, vimentin and S100B markers, related with neural progenitor and glial cells, respectively. The acute SHED transplantation promoted functional recovery, measured as from the first week after spinal cord contusion by Basso, Beattie, and Bresnahan scale. Twenty-four and 48 h after lesion, flow cytometry revealed a spinal cord vimentin+ cells increment in the SHED group. The increase of vimentin+ cells was confirmed by immunofluorescence. Moreover, the bioavailability of astrocytic proteins such as S100B and Kir4.1 shown to be increased in the spinal cord of SHED group, whereas there was a glial scar reduction, as indicated by ELISA and Western blot techniques. The presented results support that SHED act as a neuroprotector agent after transplantation, probably through paracrine signaling to reduce glial scar formation, inducing tissue plasticity and functional recovery.

Synaptophysin and caspase-3 expression on lumbar segments of spinal cord after sensorimotor restriction during early postnatal period and treadmill training.

The purpose of the current study was to investigate whether locomotor stimulation training could have beneficial effects on spinal cord plasticity consequent to sensorimotor restriction (SR). Male Wistar rats were exposed to SR from postnatal day 2 (P2) to P28. Control and experimental rats underwent locomotor stimulation training in a treadmill from P31 to P52. The intensity of the synaptophysin and caspase-3 immunoreaction was determined on ventral horn of spinal cord. The synaptophysin immunoreactivity was lower in the ventral horn of sensorimotor restricted rats compared to controls animals and was accompanied by an increased caspase-3 immunoreactivity. Those alterations were reversed at the end of the training period. Our results suggest that immobility affects the normal developmental process that spinal cord undergoes in early postnatal life influencing both pro-apoptotic and synapse markers. Also, we demonstrated that this phenomenon was reversed by 3 weeks of treadmill training.

Neuroprotector effect of stem cells from human exfoliated deciduous teeth transplanted after traumatic spinal cord injury involves inhibition of early neuronal apoptosis.

Stem cells from human exfoliated deciduous teeth (SHED) transplants have been investigated as a possible treatment strategy for spinal cord injuries (SCI) due to their potential for promoting functional recovery. The aim of present study was to investigate the effects of SHED on neuronal death after an experimental model of SCI. METHODS: Wistar rats were spinalized using NYU impactor®. Animals were randomly distributed into 4 groups: Control (Naive) or Surgical control, Sham (laminectomy with no SCI); SCI (laminectomy followed by SCI, treated with vehicle); SHED (SCI treated with intraspinal transplantation of 3×105 SHED, 1h after SCI). Functional evaluations and morphological analysis were performed to confirm the spinal injury and the benefit of SHED transplantation on behavior, tissue protection and motor neuron survival. Flow cytometry of neurons, astrocytes, macrophages/microglia and T cells of spinal cord tissue were run at six, twenty-four, forty-eight and seventy-two hours after lesion. Six hours after SCI, ELISA and Western Blot were run to assess pro- and anti-apoptotic factors. The SHED group showed a significant functional improvement in comparison to the SCI animals, as from the first week until the end of the experiment. This behavioral protection was associated with less tissue impairment and greater motor neuron preservation. SHED reduced neuronal loss over time, as well as the overexpression of pro-apoptotic factor TNF-α, while maintained basal levels of the anti-apoptotic BCL-XL six hours after lesion. Data here presented show that SHED transplantation one hour after SCI interferes with the balance between pro- and anti-apoptotic factors and reduces early neuronal apoptosis, what contributes to tissue and motor neuron preservation and hind limbs functional recovery.

Beneficial effects of early environmental enrichment on motor development and spinal cord plasticity in a rat model of cerebral palsy.

Cerebral palsy (CP) results from nonprogressive lesions in the immature brain generating changes on the neuromuscular system. Environmental enrichment (EE) is a combination of stimuli that provides greater motivation and interest in novel movement exploration through the provision of various devices associated to enhanced social stimulation that would mimic the physiotherapy approach. The aim of this study was to verify whether EE is able to prevent the establishment of motor impairment in a CP rat model. The animals were divided in two groups: control animals (healthy) and animals submitted to a CP model. After this, the pups were exposed to two environments: enriched or standard, totaling four groups: Control group (without CP in a standard environment), CP group (CP model in a standard environment), EE group (without CP in an enriched environment) and CP-EE (CP model in an enriched environment). The experimental model was induced in pregnant Wistar rats by the association of maternal exposure to bacterial endotoxin, perinatal anoxia and sensorimotor restriction of the pups. The assessment of motor skills was held using the following tests: open field, rotarod, horizontal ladder, narrow suspended bar and stride length. The histological analysis evaluated the mean cross-sectional area (CSA) of the soleus muscle fibers, the mean CSA of motoneuronal somata and expression of synaptophysin in the ventral horn of the spinal cord. EE was able to prevent the motor deficits, however, it did not reverse the muscle atrophy observed in CP animals. Furthermore, there was an average increase in the mean area of motoneurons and an increase in the expression of synaptophysin in the ventral horn of the spinal cord of the CP-EE group in relation to CP animals reared in a standard environment. Hereupon, the stimulus increment provided by EE can prevent the onset of motor deficits and histological changes in a CP rat model.

Capacidade pulmonar em idosos praticantes de hidroginástica / Lung capacity inelderly practitioners of hydrogymnastics

Objetivo: avaliar a capacidade pulmonar de um grupo de idosospraticantes de hidroginástica. Materiais e Métodos: estudo do tipolevantamento, desenvolvido com 132 indivíduos pertencentes a umgrupo da terceira idade praticante de hidroginástica na UniversidadeFederal de Santa Maria, de maio a junho de 2008. Foram utilizados,para a avaliação respiratória, uma ficha com dados gerais, espirometriae manovacuometria, sendo os dados analisados descritivamente,e após, para análise estatística, foi realizado o Teste Exato deFischer (p< 0,05). Resultados: Na avaliação espirométrica, 46,97%dos idosos apresentaram-se dentro dos parâmetros da normalidade.Já, em relação à força muscular respiratória, a maioria dosvalores encontrados de Pressão Inspiratória Máxima (92,42%) e dePressão Expiratória Máxima (59,09%) estava abaixo dos valores previstos.Porém, salienta-se que, o grupo com mais tempo de práticade hidroginástica obteve 66,67% de Pressões Expiratórias Máximasdentro dos valores previstos. No grupo de idosos que não declarouter patologias respiratórias 46,30% apresentou alteração no exameespirométrico. Conclusão: os resultados encontrados apontam paraum perfil respiratório característico do processo de envelhecimento,com perdas em capacidade pulmonar e força de músculos respiratórios,porém minimizadas pela prática de atividade física regular.(AU)

Objective: to evaluate the lung capacity of an elderly practitionergroup of hydrogymnastics. Materials and Methods: survey study,developed with 132 individuals belonging to an elderly practitionergroup of hydrogymnastics at Universidade Federal de Santa Maria,from May to June of 2008. It was used for the respiratory evaluationa sheet for general data, spirometry and manovacuometry, being thedata analyzed descriptively, and later, for statistical analysis, it wasused Fischer’s Exact Test (p <0.05). Results: In the spirometric evaluation,46.97% of the elderly showed normal parameters. However,in relation to the respiratory muscle strength, most of the found valuesof maximum inspiratory pressure (92.42%) and maximum expiratorypressure (59.09%) were below the predicted levels. However,the group with longer practice of hydrogymnastics obtained 66.67%of maximum expiratory pressure in these predicted values. In theelderly group that declared not to have any respiratory diseases,46.30% showed alteration in the spirometry exam. Conclusion: thefinding results indicate a typical respiratory profile characterized by the aging process, with losses in lung capacity and strength of respiratorymuscles, but minimized by the practice of regular physicalactivity.(AU)

Capacidade pulmonar em idosos praticantes de hidroginástica / Lung capacity inelderly practitioners of hydrogymnastics

Objetivo: avaliar a capacidade pulmonar de um grupo de idosospraticantes de hidroginástica. Materiais e Métodos: estudo do tipolevantamento, desenvolvido com 132 indivíduos pertencentes a umgrupo da terceira idade praticante de hidroginástica na UniversidadeFederal de Santa Maria, de maio a junho de 2008. Foram utilizados,para a avaliação respiratória, uma ficha com dados gerais, espirometriae manovacuometria, sendo os dados analisados descritivamente,e após, para análise estatística, foi realizado o Teste Exato deFischer (p< 0,05). Resultados: Na avaliação espirométrica, 46,97%dos idosos apresentaram-se dentro dos parâmetros da normalidade.Já, em relação à força muscular respiratória, a maioria dosvalores encontrados de Pressão Inspiratória Máxima (92,42%) e dePressão Expiratória Máxima (59,09%) estava abaixo dos valores previstos.Porém, salienta-se que, o grupo com mais tempo de práticade hidroginástica obteve 66,67% de Pressões Expiratórias Máximasdentro dos valores previstos. No grupo de idosos que não declarouter patologias respiratórias 46,30% apresentou alteração no exameespirométrico. Conclusão: os resultados encontrados apontam paraum perfil respiratório característico do processo de envelhecimento,com perdas em capacidade pulmonar e força de músculos respiratórios,porém minimizadas pela prática de atividade física regular.

Objective: to evaluate the lung capacity of an elderly practitionergroup of hydrogymnastics. Materials and Methods: survey study,developed with 132 individuals belonging to an elderly practitionergroup of hydrogymnastics at Universidade Federal de Santa Maria,from May to June of 2008. It was used for the respiratory evaluationa sheet for general data, spirometry and manovacuometry, being thedata analyzed descriptively, and later, for statistical analysis, it wasused Fischer’s Exact Test (p <0.05). Results: In the spirometric evaluation,46.97% of the elderly showed normal parameters. However,in relation to the respiratory muscle strength, most of the found valuesof maximum inspiratory pressure (92.42%) and maximum expiratorypressure (59.09%) were below the predicted levels. However,the group with longer practice of hydrogymnastics obtained 66.67%of maximum expiratory pressure in these predicted values. In theelderly group that declared not to have any respiratory diseases,46.30% showed alteration in the spirometry exam. Conclusion: thefinding results indicate a typical respiratory profile characterized by the aging process, with losses in lung capacity and strength of respiratorymuscles, but minimized by the practice of regular physicalactivity.