Corrigendum: Cloning and expression of cockroach α7 nicotinic acetylcholine receptor subunit.

[This corrects the article DOI: 10.3389/fphys.2020.00418.].

Genitourinary syndrome of menopause in patients with breast cancer treated by hormonotherapy: women's perception in ITAC, a French web-based survey.

OBJECTIVE: To describe frequency, intensity and impact of genito-urinary syndrome of menopause (GUSM) in breast cancer (BC) survivors receiving hormonotherapy (HT). METHODS: Web-based survey hosted on the Seintinelles website (database of patients and caregivers). Specific questionnaire of 43 questions, including sociodemographic characteristics, history of BC, characteristics of HT, side effects other than GUSM, symptoms of GUSM (frequency, intensity, treatment, and impact) and overall expectations regarding treatment. RESULTS: Among 1157 participants, 96.4% had at least one GUSM symptom. Percentages with at least one urinary, gynecological, or sexual symptom were 56.0%, 85.6% and 86.1% respectively while 70.3% and 10% declared at least 5 and 10 symptoms respectively. Mean (SD) and median (range) numbers of symptoms were 5.9 (2.8) and 6 (0-14) respectively. Most frequently reported symptoms were decreased desire (77.8%), decreased arousal (71.4%), and vaginal dryness 68.4%). On a scale from 0 (no impact) to 10 (maximal impact), the most important impact was reported for sexual life (mean: 6.6±3.5) followed by psychological condition/self-image (mean 5.4±3.1), and relations with partners (mean: 5.1±3.4). Only 13.6% of participants had received information on GUSM prior to the survey. CONCLUSIONS: GUSM remains underdiagnosed and underestimated in BC survivors who receive HT, although it is among most frequent and disabling side effects of HT. Awareness should be increased among physicians, along with information to women. Early detection and treatment of symptoms and prophylaxis of GUSM in at-risk women should be implemented.

Cloning and Expression of Cockroach α7 Nicotinic Acetylcholine Receptor Subunit.

Understanding insect nicotinic acetylcholine receptor (nAChR) subtypes is of major interest because they are the main target of several insecticides. In this study, we have cloned a cockroach Pameα7 subunit that encodes a 518 amino acid protein with futures typical of nAChR subunit, and sequence homology to α7 subunit. Pameα7 is differently expressed in the cockroach nervous system, in particular in the antennal lobes, optical lobes and the mushroom bodies where specific expression was found in the non-compact Kenyon cells. In addition, we found that cockroach Pameα7 subunits expressed in Xenopus laevis oocytes can assemble to form homomeric receptors. Electrophysiological recordings using the two-electrode voltage clamp method demonstrated that nicotine induced an I max current of -92 ± 27 nA at 1 mM. Despite that currents are low with the endogenous ligand, ACh, this study provides information on the first expression of cockroach α7 homomeric receptor.

Neonicotinoid insecticides differently modulate acetycholine-induced currents on mammalian α7 nicotinic acetylcholine receptors.

BACKGROUND AND PURPOSE: Neonicotinoid insecticides are described as poor agonists of mammalian nicotinic ACh receptors. In this paper, we show that their effects on mammalian nicotinic receptors differ between compounds. EXPERIMENTAL APPROACH: Two-electrode voltage-clamp electrophysiology was used to characterize the pharmacology of three neonicotinoid insecticides on nicotinic α7 receptors expressed in Xenopus oocytes. Single and combined application of clothianidin, acetamiprid and thiamethoxam were tested. RESULTS: Two neonicotinoid insecticides, clothianidin and acetamiprid, were partial agonists of mammalian neuronal α7 nicotinic receptors, whereas another neonicotinoid insecticide, thiamethoxam, which is converted to clothianidin in insect and plant tissues, had no effect. Pretreatment with clothianidin and acetamiprid (10 µM) ACh significantly enhanced the subsequent currents evoked by ACh (100 µM ) whereas pretreatment with thiamethoxam (10 µM) reduced ACh-induced current amplitudes.A combination of the three neonicotinoids decreased the ACh-evoked currents. CONCLUSIONS AND IMPLICATIONS: The present findings suggest that neonicotinoid insecticides differ markedly in their direct effects on mammalian α7 nicotinic ACh receptors and can also modulate ACh-induced currents. Furthermore, our data indicate a previously unknown modulation of mammalian α7 nicotinic receptors by a combination of clothianidin, acetamiprid and thiamethoxam. LINKED ARTICLES: This article is part of a themed section on Nicotinic Acetylcholine Receptors. To view the other articles in this section visit http://onlinelibrary.wiley.com/doi/10.1111/bph.v175.11/issuetoc.

Psychometric Evaluation of a Shortened Version of the 40-item Defense Style Questionnaire

The 40-item version of the Defense Style Questionnaire (DSQ-40) is a widely used self-report measure of defense mechanisms. A previous study, however, has suggested that 12 items out of 40 are lacking in face validity. The aim of the present study was to evaluate the psychometric properties of a shortened version of the DSQ-40, deleting the items with insufficient face validity (DSQ-28), in a non-clinical sample of university students. Exploratory factor analysis extracted a five-factor structure. The pattern of relationships for the five factors along with coping strategies assessed by the Ways of Coping Checklist, short version (WCCL), general psychopathological symptoms, evaluated by the Symptom Checklist 90-Revised (SCL-90 R), and personality disorder symptoms, explored through the Personality Diagnostic Questionnaire, Fourth Edition (PDQ-4), suggests that this 28 item-version of the DSQ may have better discriminant and criterion validity than the DSQ-40 (AU)

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Psychopathic traits and suicidal ideation in high-school students.

The aim of the study was to evaluate the contribution of psychopathic traits in the prediction of suicidal ideation among non-clinical non-forensic adolescents. A sample of 312 high-school students completed questionnaires assessing suicidal ideation, depressive symptoms, borderline personality traits, and cannabis use and the Youth Psychopathic traits Inventory (YPI, Andershed, Kerr, Stattin et al., 2002), which consists of three dimensions, the Interpersonal subscale (grandiosity, manipulation), the Affective subscale (callousness, unemotionality), and the Behavioral subscale (impulsiveness, irresponsibility). A multiple regression analysis showed that the affective component of psychopathic traits was an independent predictor of suicidal ideation. Our results suggest that clinicians should not assume that the presence of psychopathic traits in adolescent is a protection against suicidal ideation.

Cannabis use and delinquent behaviors in high-school students.

The aim of the study was to evaluate the relative contributions of cannabis use, alcohol use, psychopathic and borderline personality traits, and depressive symptoms in the prediction of delinquent behaviors. Participants were 312 high-school students who completed self-report questionnaires. Hierarchical multiple regression analyses were conducted to investigate the contribution of cannabis use and potential confounding variables to delinquent behaviors in the total sample and in cannabis users. Cannabis use was no more a significant independent predictor of delinquent behaviors after adjustment for alcohol use and psychopathological variables. However, among users, frequency of use remained a significant predictor of delinquency after adjustment for those confounders.