[Study of enzymes of xenobiotic metabolism in the evaluation of quality of protein-containing wheat germ flakes and wallpaper flour]. / Issledovanie fermentov metabolizma ksenobiotikov dlia otsenki kachestva belka pshenichnykh zarodyshevykh khlop'ev i oboinoi muki.

Intake of wheat upholstery meal by growing rats was attended by a sharp decrease in the content and activity of xenobiotic metabolism enzymes in the hepatic microsomes, that was caused by the low biological value of the meal proteins. Hepatic microsomes of the rats that were fed with wheat germ flakes showed increased specific content of cytochromes P-450 and b5, but the total blood protein content per 100 g of body mass was lower than during casein consumption. No significant changes were detected in hydroxylation rate of benz(a)pyrene, aniline and ethylmorphine. During consumption of wheat germ flakes induction of UDP-glucuronide-transferase was detected in hepatic microsomes. Wheat germ flakes induced a 5-fold increase of Se-dependent glutathione peroxidase activity. Wheat germ flakes produced no significant effect on glutathione-S-aryltransferase and glutathione reductase activity.

[Effect of dimethylformamide on the microsomal hydroxylation system in the liver of rats fed diets with different fat contents]. / Vliianie dimetilformamida na sistemu mikrosomal'nogo gidroksilirovaniia pecheni krys, potrebliavshikh ratsionu s kachestvenno razlichnymi zhirami.

The authors studied the effect of the universal aprotonic solvent dimethylformamide (DFA) on liver microsomal hydroxylation of male rats fed diets with different fats (sunflower oil or lard). DMFA given in a dose of 200 mg/kg bw per os for 4 weeks produced a considerable reduction in cytochrome P-450 content in liver homogenates and microsomes and a lowering of the activity of N-demethylation in microsomes with amidopyrine, ethylmorphine and DMFA being used as substrates. The degree of inhibition of the demethylase reactions and liver cytochrome P-450 content was more demonstrable in rats fed the diet containing sunflower oil as compared with animals whose diet contained lard. During DMFA intoxication, the rate of spontaneous formation of malonic dialdehyde in liver homogenates of rats given sunflower oil was considerably higher than in rats fed the diet with lard. Remarkable coproporphyrinuria was revealed during DMFA intoxication which was more pronounced in rats fed the diet containing sunflower oil. It was demonstrated that the lard-containing diet was marked by a higher vitamin E/polyunsaturated fatty acids ratio (mg/100 g) with regard to alpha-tocopherol acetate added to the diet as compared to the sunflower oil-containing diet. The role of antioxidant properties of the diets in modification of the hepatotoxic action of DMFA is discussed.

[Ascorbic acid content in the blood serum and lymphoid organs in antigenic exposure]. / Soderzhanie askorbinovoi kisloty v syvorotke krovi i limfoidnykh organov pri antigennom vozdeistvii.

Seven and 14 days following immunization with sheep red blood cells, the guinea-pigs incapable of synthesizing ascorbic acid showed its pronounced decrease in blood serum accompanied by elevation of ascorbate content in the thymus and spleen, central organs responsible for immunogenesis. Immunization of rats capable of synthesizing ascorbic acid did not lead to its changes in the blood serum, thymus or spleen.