ABSTRACT
We describe here a very simple modification of the auramine staining procedure based on preparation of a UV-fixed thick blotch which allowed us to reach an overall sensitivity of 0.82 (592 acid-fast bacillus [AFB]-positive specimens/722 initial respiratory specimens with positive mycobacterial culture) and sensitivities of 0.93 (526 AFB-positive specimens/564 culture-positive specimens) for Mycobacterium tuberculosis complex and 0.42 (66 AFB-positive specimens/158 culture-positive specimens) for nontuberculous mycobacteria.
Subject(s)
Bacteriological Techniques/methods , Mycobacterium/isolation & purification , Specimen Handling/methods , Sputum/microbiology , Staining and Labeling/methods , Tuberculosis/diagnosis , Benzophenoneidum/metabolism , Humans , Sensitivity and SpecificityABSTRACT
In the course of an ethnobotanical research project carried out in the Province of Granada (Southern Spain), 267 taxa have been catalogued as being used in folk medicine. Based on the literature consulted, phytotherapeutic use for 34 of these was recorded for the first time.