ABSTRACT
Five new diterpenes (1-5) and a megastigmane derivative (6) were isolated from the aerial parts of Euphorbia laurifolia, along with several known compounds. Their structures were elucidated by NMR, MS, and ECD and by chemical methods. A chemical proteomics drug affinity responsive target stability (DARTS) approach to investigate the lathyrane diterpene 1, laurifolioside, on its putative cellular target(s) was performed. Clathrin heavy chain 1, a protein mainly involved in selective uptake of proteins, viruses, and other macromolecules at the plasma membrane of cells, was identified as the major interaction partner of compound 1. The modulation of clathrin activity by 1 was studied through microscopy, molecular docking, and molecular dynamics studies, suggesting a new activity of lathyrane diterpenes in the modulation of trafficking pathways.
Subject(s)
Clathrin Heavy Chains/metabolism , Diterpenes/isolation & purification , Diterpenes/pharmacology , Euphorbia/chemistry , Cyclohexanones/chemistry , Cyclohexanones/isolation & purification , Cyclohexanones/pharmacology , Diterpenes/chemistry , Ecuador , Glucosides/chemistry , Glucosides/isolation & purification , Glucosides/pharmacology , Humans , Molecular Docking Simulation , Molecular Structure , Norisoprenoids/chemistry , Norisoprenoids/isolation & purification , Norisoprenoids/pharmacology , Nuclear Magnetic Resonance, Biomolecular , Plant Components, Aerial/chemistry , Plant Proteins/analysis , Plant Proteins/chemistryABSTRACT
A phytochemical analysis of Lantana fucata dried leaves led to the isolation of three new phenylethanoid glycosides, fucatosides A-C, along with parvifloroside A and six known methoxyflavones. Their structures were established by NMR and ESIMS experiments. In vitro assays showed that the alcoholic extract and fucatoside C have significant anti-inflammatory effects, inhibiting NO release in the LPS-induced J774.A1 murine macrophage cell line.