Gas Identification by Simultaneous Permeation through Parallel Membranes: Proof of Concept.

This paper describes an experimental system for simultaneous permeation of a pressurized test gas through different gas permeable membranes and provides a proof of concept for a novel approach for gas identification/fingerprinting for potential construction of electronic noses. The design, construction, and use of a six-channel system which allows simultaneous gas permeation from a single pressurized gas compartment through six different parallel membranes are presented. The permeated gas is accumulated in confined spaces behind the respective membranes. The rate of gas pressure accumulation behind each membrane is recorded and used as a measure of the gas permeation rate through the membrane. The utilized gas permeable membranes include Teflon AF, silicone rubber, track-etch hydrophilic polycarbonate, track-etch hydrophobic polycarbonate, track-etch polyimide, nanoporous anodic aluminum oxide, zeolite ZSM-5, and zeolite NaY. An analogy between the rate of pressure accumulation of the permeating gas behind the membrane and the charging of an electric capacitor in a single series RC circuit is proposed and thoroughly validated. The simultaneous permeation rates through different membranes demonstrated a very promising potential as characteristic fingerprints for 10 test gases, that is, helium, neon, argon, hydrogen, nitrogen, carbon dioxide, methane, ethane, propane, and ethylene, which are selected as representative examples of mono-, di-, tri-, and polyatomic gases and to include some homologous series as well as to allow testing the potential of the proposed system to discriminate between closely related gases such as ethane and ethylene or carbon dioxide and propane which have almost identical molecular masses. Finally, a preliminary investigation of the possibility of applying the developed gas permeation system for semiquantitative analysis of the CO2-N2 binary mixture is also presented.

An Online Management System for Streamlining and Enhancing the Quality of Learning Outcomes Assessment.

Learning outcomes assessment is an effective academic quality assurance tool that enables educators to review and enhance the alignment between planned, delivered, and experienced curricula. Accurately assessing what students know and are able to do after completing a learning module is the first step to decide on the strategies to implement and the proper actions to take in order to ensure the continuous improvement of the student learning experience. Nonetheless, learning outcomes assessment processes in higher education are still facing major challenges that affect their proper and effective implementation. Hence, faculty do not usually experience noticeable improvement in the students' performance over several assessment cycles, which causes their frustration and reluctance to continue participating in the assessment process. This paper discusses the main issues that affect the implementation of the assessment process and prevent the closure of the assessment loop. It also introduces a unified assessment process and an online management system that have been developed recently to address the discussed issues. The online management system streamlines the assessment process, while providing administrators and quality assurance officers with valuable infographics and reports to effectively oversee the implementation of the assessment process. The system has been deployed at the United Arab Emirates University since fall 2018, and has been successfully used by faculty to assess the learning outcomes for more than 1000 courses each semester. Moreover, collected statistics showed that the online features provided by the system allowed faculty to continue their assessment tasks seamlessly during the COVID-19 pandemic.

Development and Characterization of Novel Flow Injection, Thin-Layer, and Batch Cells for Electroanalytical Applications Using Screen-Printed Electrodes.

In the present paper, the design, fabrication, and analytical applications of three novel cells for flow injection, thin-layer, and batch electrochemical measurements using screen-printed electrode chips (SPECs) are described. Each cell consisted of an acrylic base and a transparent acrylic cover. The essential construction feature of each cell base was a cavity to accommodate the SPEC, whereas the construction features of the clear acrylic cover determined the cell shape and its function. The presented cells offered several common advantages, which include (i) convenient electrical connection of the SPEC to any potentiostat without the need for special cables, (ii) the SPEC was completely contained within the cell body, which eliminated the risk of its breakage, (iii) suitable for use with a large number of commercially available SPECs, and (iv) excellent SPEC sealing. The flow cell offered additional advantages of convenient customization of the cell dead volume and convenient visual inspection of the surface and the vicinity of SPEs. The presented thin-layer cell is the first report on a dedicated cell which realized a near-ideal thin-layer steady-state voltammetry using SPECs. The universal batch cell (UBC) offered extreme versatility and proved suitable for all batch applications in sample volumes ranging from 25 µL to 40 mL with an optional controlled temperature and atmosphere. Moreover, a novel way to achieve stirred-solution chronoamperometry and hydrodynamic voltammetry using SPECs (with superior signal-to-noise ratios) using the UBC is described. Electrochemical measurements to demonstrate the merits and the applicability of all cells are also presented.

Vitamin D Is Necessary for Murine Gastric Epithelial Homeostasis.

Unlike other organs, the importance of VD in a normal stomach is unknown. This study focuses on understanding the physiological role of vitamin D in gastric epithelial homeostasis. C57BL/6J mice were divided into three groups that were either fed a standard diet and kept in normal light/dark cycles (SDL), fed a standard diet but kept in the dark (SDD) or fed a vitamin D-deficient diet and kept in the dark (VDD). After 3 months, sera were collected to measure vitamin D levels by LC-MS/MS, gastric tissues were collected for immunohistochemical and gene expression analyses and gastric contents were collected to measure acid levels. The VDD group showed a significant decrease in the acid-secreting parietal cell-specific genes Atp4a and Atp4b when compared with the controls. This reduction was associated with an increased expression of an antral gastrin hormone. VDD gastric tissues also showed a high proliferation rate compared with SDL and SDD using an anti-BrdU antibody. This study indicates the requirement for normal vitamin D levels for proper parietal cell functions.

Prototype amperometric biosensor for sialic acid determination.

This paper describes the first report on the development, characterization, and applications of a prototype amperometric biosensor for free sialic acid (SA). The sensor was constructed by the coimmobilization of two enzymes, i.e., N-acetylneuraminic acid aldolase and pyruvate oxidase, on a polyester microporous membrane, which was then mounted on top of a platinum disk electrode. The SA biosensor operation was based on the sequential action of the two enzymes to ultimately produce hydrogen peroxide, which was then detected by anodic amperometry at the platinum electrode. The surface of the platinum electrode was coated with an electropolymeric layer to enhance the biosensor selectivity in the presence of interfering oxidizable species. Optimization of the enzyme layer composition resulted in a fast and steady current response in phosphate buffer pH 7.2 at 37 degrees C. The limit of detection was 10 microM, and the response was linear to 3.5 mM (r = 0.9987). The prepared SA biosensors retained approximately 85% of their initial sensitivity after 8 days and showed excellent response reproducibility (CV = 2.3%). Utilization of a third enzyme, sialidase, expanded the scope of the present SA biosensor to determine bound sialic acid as well. The merits of the described biosensor allowed its successful application in determining SA in biological and pharmaceutical samples. The obtained results indicated that the presented SA biosensor should be a useful bioanalytical tool in several biological and clinical applications such as screening of SA as a nonspecific tumor marker as well as monitoring of tumor therapy.

A novel 1,10-phenanthroline-sensitive membrane sensor for potentiometric determination of Hg(II) and Cu(II) cations.

Preparation, characterization, and applications of a 1,10-phenanthrolinium cation (phenH(+))-sensitive potentiometric sensor are described. The sensor incorporates a liquid polymeric membrane consisting of phenH-tetraphenylborate, nitrophenyloctyl ether, and poly(vinyl chloride) as ion exchanger, plasticizer, and polymeric support, respectively. The sensor exhibits a fast and Nernstian response to phenH(+) over the concentration range of 6 x 10(-6)-2 x 10(-4) M with a monovalent cationic slope of 58.0+/-0.5 mV/log[phenH(+)] in acetate buffer of pH 4.2. The sensor is successfully applied to the monitoring of the potentiometric titration of Hg(II) and Cu(II) ions with phen solution in the presence of citrate and acetate buffers of pH 4.2, respectively. Sharp inflection breaks (90-180 mV) at 1:1 (metal:phen reaction) are obtained in the presence of chloride and thiocyanate background. This stoichiometry is explained by the formation of insoluble [HgCl(2)(phen)], [Hg(SCN)(2)(phen)], and [Cu(SCN)(2)(phen)] complexes. Optimization of each titration and the effect of foreign ions are evaluated. The method offers the advantages of adequate sensitivity, accuracy, and selectivity for the determination of mercury and copper in pharmaceutical, rock, and tea samples. The results are in good agreement with those obtained using the standard atomic absorption spectrometric and United States Pharmacopeial methods.

Improved electrodeposited iridium oxide pH sensor fabricated on etched titanium substrates.

In the present paper, the preparation and characterization of an improved solid-state pH sensor are described. The sensor is based on anodically electrodeposited iridium oxide film, as a pH-sensing layer. Merits of the present sensor include (i) excellent adhesion of the pH sensitive layer to the substrate, (ii) excellent reproducibility of sensor fabrication, (iii) faster preparation procedure, and (iv) low cost of the titanium substrate. These advantages are realized by combining acid-etched titanium as the electrode substrate with an optimized electrodeposition solution consisting of IrCl4 as an iridium source, hydrogen peroxide, potassium oxalate, and potassium carbonate. Heating the electrodeposition solution to 90 degrees C reduced the time required for solution development from approximately 3 days to 10 min. The pH-sensing layer is protected with a layer of Nafion and a microporous polyester membrane. The improved sensor showed a super-Nernstian response (-73.7 +/- 1.2 mV/pH unit) in the pH range of 1.5-11.5. The present pH sensor, fabricated in a tubular form, is used as a detector in a flow injection analysis (FIA) system for pH measurements. Optimization of the FIA experimental parameters resulted in a linear dependence of peak heights on the pH of the injected samples in the pH range of 2-11.

Serum fluoride levels in a group of Egyptian infants and children from Cairo city.

In this study, the authors investigated fluoride levels in the serum of infants and children (n = 296) and in the breast milk from nursing mothers (n = 60) in Cairo city. Their goal was to evaluate the necessity and safety of implementing a fluoride supplementation program. The authors used an ion-selective electrode to assay fluoride by direct potentiometry. Also, 2- to 12-yr-old participants underwent clinical dental examinations to detect caries and/or fluorosis. The serum fluoride levels of infants were significantly lower than levels found in preschoolers and school-age children. Serum fluoride correlated positively with age; it was significantly lower during the 1st than 2nd yr of infancy (p = 0.005). Breast or formula feeding did not influence serum fluoride status; the fluoride levels in mothers' milk reflected the serum levels of their own infants. Dental examinations revealed that 81% of the children had caries, whereas there was no evidence of fluorosis. Serum fluoride levels did not vary with the presence or absence of dental caries and did not correlate with the number of decayed, missing, or filled teeth. Gender did not influence serum fluoride expression, and the percentile values were unrelated to height, weight, or head circumference. These findings suggest the necessity and safety of improving the fluoride consumption levels of infants and children in Cairo city. Wider-scale studies are needed to obtain better insight into the problem.

Selective potentiometric determination of nitrite ion using a novel (4-sulphophenylazo-)1-naphthylamine membrane sensor.

A novel polymeric membrane sensor sensitive to (4-sulphophenylazo-)1-naphthylamine (SPAN) based on the use of tris(bathophenanthroline) Ni(II)-SPAN ion pair as an ion exchanger in plasticised PVC membrane is described. The sensor exhibits a linear calibration plot with near-Nernstian anionic slope of -55.0+/-0.3 mV log[SPAN](-1) over the concentration range 10(-6)-10(-2) mol l(-1) at pH 7. The sensor shows working range over the pH 6-8, response time of 20 s for 10(-5) mol l(-1) and operational lifetime of 8 weeks. The sensor is used for quantification of micro quantities of nitrite ion by a prior conversion into the more lipophilic SPAN ion, which is measured with adequate sensitivity, and high selectivity using SPAN sensor. Validation of the method according to the quality assurance standards shows good performance characteristics. The sensor is satisfactory utilised for potentiometric determination of nitrite ion in wastewater samples and meat products. The results are favourably compared with data obtained using the standard spectrophotometric procedure involving the same reaction.

Measurement of extracellular pH, K(+), and lactate in ischemic heart.

Simultaneous and continuous measurements of extracellular pH, potassium (K(+)), and lactate (L(-)) in ischemic rabbit papillary muscle are presented for the first time. Potentiometric pH and K(+) sensors and an amperometric lactate biosensor were used. These miniature electrodes were previously developed and individually tested for this purpose. The pH sensor was based on an iridium oxide layer electrodeposited on a planar platinum electrode fabricated on a flexible substrate. The potentiometric K(+) sensor was based on a polymeric membrane and valinomycin ionophore. The L(-) biosensor was based on lactate oxidase and an organic conducting salt polarized at 0.15V vs Ag/AgCl reference electrode. The utility of this novel analytical system to cardiovascular research was demonstrated by using the system to study the interrelationship of cellular K(+) and lactate loss in ischemic myocardium, and the role of extracellular pH and buffer capacity on this relationship. The results indicated: (i) sequential brief episodes of ischemia produced reproducible trends of L(-), pH, and K(+) changes during the first three episodes, (ii) extracellular L(-) increased with increasing buffer capacity of extracellular compartment, (iii) the patterns of extracellular L(-) and K(+) changes were not related directly, and (iv) L(-) transport and lactic acid diffusion were not the primary cause of extracellular acidosis during ischemia.