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Arkh Patol ; 76(2): 46-7, 2014.
Article in Russian | MEDLINE | ID: mdl-25051727

ABSTRACT

Optimal conditions were defined for DNA isolation from formalin-fixed and paraffin-embedded (FFPE) archived autopsy lung tissue slices. The quality of DNA preparations isolated from the FFPE archived slices was assessed and their suitability for further molecular genetic analysis estimated. DNA isolated from the FFPE slices stored less and more than 10 years was suitable for molecular genetic studies in 100 and 66.7% of cases, respectively.


Subject(s)
DNA/genetics , DNA/isolation & purification , Paraffin Embedding , Autopsy , Formaldehyde , Humans , Lung/chemistry , Lung/pathology , Tissue Fixation
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