Treatment and prevention of pathological mitochondrial dysfunction in retinal degeneration and in photoreceptor injury.

Pathological deterioration of mitochondrial function is increasingly linked with multiple degenerative illnesses as a mediator of a wide range of neurologic and age-related chronic diseases, including those of genetic origin. Several of these diseases are rare, typically defined in the United States as an illness affecting fewer than 200,000 people in the U.S. population, or about one in 1600 individuals. Vision impairment due to mitochondrial dysfunction in the eye is a prominent feature evident in numerous primary mitochondrial diseases and is common to the pathophysiology of many of the familiar ophthalmic disorders, including age-related macular degeneration, diabetic retinopathy, glaucoma and retinopathy of prematurity - a collection of syndromes, diseases and disorders with significant unmet medical needs. Focusing on metabolic mitochondrial pathway mechanisms, including the possible roles of cuproptosis and ferroptosis in retinal mitochondrial dysfunction, we shed light on the potential of α-lipoyl-L-carnitine in treating eye diseases. α-Lipoyl-L-carnitine is a bioavailable mitochondria-targeting lipoic acid prodrug that has shown potential in protecting against retinal degeneration and photoreceptor cell loss in ophthalmic indications.

Pathogenic mitochondrial dysfunction and metabolic abnormalities.

Herein we trace links between biochemical pathways, pathogenesis, and metabolic diseases to set the stage for new therapeutic advances. Cellular and acellular microorganisms including bacteria and viruses are primary pathogenic drivers that cause disease. Missing from this statement are subcellular compartments, importantly mitochondria, which can be pathogenic by themselves, also serving as key metabolic disease intermediaries. The breakdown of food molecules provides chemical energy to power cellular processes, with mitochondria as powerhouses and ATP as the principal energy carrying molecule. Most animal cell ATP is produced by mitochondrial synthase; its central role in metabolism has been known for >80 years. Metabolic disorders involving many organ systems are prevalent in all age groups. Progressive pathogenic mitochondrial dysfunction is a hallmark of genetic mitochondrial diseases, the most common phenotypic expression of inherited metabolic disorders. Confluent genetic, metabolic, and mitochondrial axes surface in diabetes, heart failure, neurodegenerative disease, and even in the ongoing coronavirus pandemic.

Meteorological patterns and the evolution of West Nile virus in an environmentally stressed Mediterranean area.

The present work investigates the increase of confirmed cases of West Nile virus and the relationship between weather-related patterns and the geographical expansion of West Nile virus in Greece, with a special focus on West Attica, Central Greece, a semi-arid, ecologically fragile Mediterranean area. Using data from the European Environment Agency, European Drought Observatory of Joint Research Centre, the pairwise relationship between surface air temperature anomalies, precipitation anomalies, soil moisture index anomalies, and the fraction of absorbed photosynthetically active radiation anomalies (fAPAR) was evaluated during summer time of 2018, a particularly intense virus outbreak. The empirical results of this study indicate that total precipitation during 2018 was extremely high, nearly 500% above the average. These conditions contributed to the increase of soil moisture index anomaly and fAPAR, creating an ideal microenvironment (wet soils and green pastures) for mosquito breeding. This phenomenon was directly associated with a drastic outbreak of West Nile virus cases in the area, compared with earlier years. Our results indicate how unusually high values of summer precipitation may have contributed (both through direct and indirect ecological channels) to the rapid spread of the West Nile virus in West Attica, causing a significant number of confirmed cases and fatalities. Climate change may bring forth other issues aside from natural disasters, including-but not limited to-virus expansion.

Perception of biohazards: a focus on schools in Western Attica, Greece.

Biological disasters endanger the lives of teachers and students, causing serious disturbances to schools, and forcing them to shut down for a short or long period of time. Over the last few decades, the Greater Athens area and Attica Prefecture in Greece have experienced several natural disasters. These events have highlighted problems and weaknesses in emergency planning for school communities at both local and regional level, and have shown the need for new precautionary measures and effective risk management for modern society and school communities. The present study reports an investigation of the perception of the risk posed by biological hazards to teacher safety. The investigation utilized a questionnaire survey of teachers working in the secondary education directorate in Western Attica, which was carried out between May and December 2019. The teachers' perceptions of the extent to which their safety was affected by biohazards (i.e., infectious diseases and weather-related diseases) were investigated using a 5-point scale ranging from affected a lot (- 2) to not affected at all (+ 2). A multivariate statistical technique-principal component analysis-was used to explore the results of the survey. The results showed that the teachers' feelings of safety were affected a lot (- 2) or affected enough (- 1) by biohazards. 61.5% (n = 72) of the participants reported that their feelings of safety were affected a lot or enough by infectious diseases, and 55.6% (n = 65) of the participants noted that their feelings of safety were affected a lot or enough by weather-related diseases. Compared to other natural, technological, or social hazards, biological hazards seem to have a greater impact on teachers' feelings of safety. These results could have implications for the design of risk management plans for school communities.

Air quality and meteorological patterns of an early spring heatwave event in an industrialized area of Attica, Greece.

Heatwaves-excessively hot ambient conditions that are considered a serious threat to human health-are often associated with poor air quality. The aim of this study was to examine the impact of an early heatwave episode in an industrialized plain in the eastern Mediterranean region (Thriasio, Greece) on human thermal discomfort and urban air quality. The heatwave occurred in mid (15-20) May 2020, shortly after some of the restrictions that were improsed to halt the spread of coronavirus disease 2019 (COVID-19) in Greece were lifted (on 4 May). The discomfort index (DI) and the daily air quality index (DAQI) were calculated on an hourly basis throughout spring 2020 (March, April, May) using data from two stations that measure meteorological parameters and air pollutant concentrations in the Thriasio Plain. The analysis showed that the air temperature increased during 7-17 May to levels that were more than 10 °C above the monthly average value (25.8 °C). The maximum measured air temperature was 38 °C (on 17 May). The results showed a high level of thermal discomfort. The DI exceeded the threshold of 24 °C for several hours during 13-20 May. Increased air pollution levels were also identified. The average DAQI was estimated as 0.83 ± 0.1 and 1.14 ± 0.2 at two monitoring stations in the region of interest during the heatwave. Particulate matter (diameter < 10 µm) appeared to contribute significantly to the poor air quality. Significant correlations between the air temperature, DI, and AQSI were also identified.

Klotho Pathways, Myelination Disorders, Neurodegenerative Diseases, and Epigenetic Drugs.

In this review we outline a rationale for identifying neuroprotectants aimed at inducing endogenous Klotho activity and expression, which is epigenetic action, by definition. Such an approach should promote remyelination and/or stimulate myelin repair by acting on mitochondrial function, thereby heralding a life-saving path forward for patients suffering from neuroinflammatory diseases. Disorders of myelin in the nervous system damage the transmission of signals, resulting in loss of vision, motion, sensation, and other functions depending on the affected nerves, currently with no effective treatment. Klotho genes and their single-pass transmembrane Klotho proteins are powerful governors of the threads of life and death, true to the origin of their name, Fates, in Greek mythology. Among its many important functions, Klotho is an obligatory co-receptor that binds, activates, and/or potentiates critical fibroblast growth factor activity. Since the discovery of Klotho a little over two decades ago, it has become ever more apparent that when Klotho pathways go awry, oxidative stress and mitochondrial dysfunction take over, and age-related chronic disorders are likely to follow. The physiological consequences can be wide ranging, potentially wreaking havoc on the brain, eye, kidney, muscle, and more. Central nervous system disorders, neurodegenerative in nature, and especially those affecting the myelin sheath, represent worthy targets for advancing therapies that act upon Klotho pathways. Current drugs for these diseases, even therapeutics that are disease modifying rather than treating only the symptoms, leave much room for improvement. It is thus no wonder that this topic has caught the attention of biomedical researchers around the world.

The 2007 crisis and Greek wildfires: a multivariate analysis of suppression times.

Shifts in government priorities in response to the 2007 global recession have affected wildfire management and natural disaster funding arrangements, leading to a reduced effectiveness of fire suppression actions and increasing fire vulnerability. Our study investigates the role of local socioeconomic contexts on fire suppression effectiveness under economic expansion and recession in a Mediterranean region (Attica, Greece) strongly affected by 2007 crisis and displaying a persistently high density of peri-urban wildfires. Basic characteristics of wildfires (spatial distribution, intensity, and land use preferences) were investigated in the study area over two consecutive 8-year time intervals characterized by economic expansion (2000-2007) and recession (2008-2015). An integrated approach based on multivariate statistics and artificial neural networks was implemented to evaluate latent relationships between fire suppression time, wildfire characteristics, and socioeconomic dynamics. Controlling for wildfires' characteristics over the two time intervals, fire time length increased under crisis-mainly for small and medium-sized fires-possibly as an indirect response to reduced effectiveness of forest land management. Local contexts and political decisions influenced by economic downturns are relevant factors shaping wildfires' severity in the Mediterranean region. With recession, local contexts vulnerable to wildfires require more effective fire prevention measures, sustainable forest management, and regional planning.

Perceived air quality and particulate matter pollution based on field survey data during a winter period.

Outdoor air pollution and especially particulate matter pollution is a major environmental health issue that raises concerns of scientists and policy makers. This study focuses on air quality perception in relation to particulate matter in order to find potential patterns. Field questionnaire-based surveys were conducted among pedestrians on two central sites in the city of Athens, Greece, during the winter period while particulate matter of 10 µm or less in diameter (PM10) were concurrently measured on-site at 1-min resolution. The participants were asked to evaluate the dust and the overall pollution-related air quality based on 5-point bipolar scales. Air quality perception patterns were explored considering PM10 concentration, meteorological and thermal conditions, and subjective variables including gender, age, smoking status, and health status. An effect of PM10 on dust perception was identified, suggesting that dusty air quality conditions are reported more frequently when particulate concentration increases. Health status, exposure time, smoking status, and gender were found to affect air quality perception. Participants experiencing health symptoms, exposed to outdoor conditions for more than 30 min, smokers, and females were more likely to report unfavorable air quality conditions. The comparison with the results of a previous study obtained using air pollution station data confirmed the dependence of air quality perception on participants' characteristics and particularly on their health status. Ordinal logistic regression models showed that for pedestrians who were neither smokers nor experiencing health symptoms and were exposed to outdoor conditions for more than 30 min, a PM10 concentration around 64 µg·m-3 could be a threshold, to perceive dusty air quality conditions.

Data on multiple body parameters, microclimatic variables, and subjective assessment of thermal sensation monitored in outdoor environment.

This paper describes two sets of data on multiple body parameters of five participants, on microclimatic variables, and on self-reported assessment of thermal responses, all monitored in the same outdoor urban environment. Data were collected during three seasons, summer, autumn and winter 2010-2011, in the city of Athens, Greece. Part of these data, collected during the summer period, is related to the research article entitled "Case study of skin temperature and thermal perception in a hot outdoor environment." (Pantavou et al., 2014) [1].

Complex Adaptive Systems, soil degradation and land sensitivity to desertification: A multivariate assessment of Italian agro-forest landscape.

Degradation of soils and sensitivity of land to desertification are intensified in last decades in the Mediterranean region producing heterogeneous spatial patterns determined by the interplay of factors such as climate, land-use changes, and human pressure. The present study hypothesizes that rising levels of soil degradation and land sensitivity to desertification are reflected into increasingly complex (and non-linear) relationships between environmental and socioeconomic variables. To verify this hypothesis, the Complex Adaptive Systems (CAS) framework was used to explore the spatiotemporal dynamics of eleven indicators derived from a standard assessment of soil degradation and land sensitivity to desertification in Italy. Indicators were made available on a detailed spatial scale (773 agricultural districts) for various years (1960, 1990, 2000 and 2010) and analyzed through a multi-dimensional exploratory data analysis. Our results indicate that the number of significant pair-wise correlations observed between indicators increased with the level of soil and land degradation, although with marked differences between northern and southern Italy. 'Fast' and 'slow' factors underlying soil and land degradation, and 'rapidly-evolving' or 'locked' agricultural districts were identified according to the rapidity of change estimated for each of the indicators studied. In southern Italy, 'rapidly-evolving' districts show a high level of soil degradation and land sensitivity to desertification during the whole period of investigation. On the contrary, those districts in northern Italy are those experiencing a moderate soil degradation and land sensitivity to desertification with the highest increase in the level of sensitivity over time. The study framework contributes to the assessment of complex local systems' dynamics in affluent but divided countries. Results may inform thematic strategies for the mitigation of land and soil degradation in the framework of action plans to combat desertification.

Biometeorological and air quality assessment in an industrialized area of eastern Mediterranean: the Thriassion Plain, Greece.

Evidence that heat wave events are associated with poor air quality conditions and health hazards has become stronger in recent years. In this study, the impact of two heat wave episodes on human thermal discomfort and air quality is examined during summer 2007, in an industrial plain of eastern Mediterranean: the Thriassion Plain, Greece. For this purpose, two biometeorological indices-Discomfort Index (DI) and Heat Load (HL)-as well as an air quality index-Air Quality Stress Index (AQSI)-were calculated using data from seven measuring sites. A land-use map was procured in order to examine the effect of different land cover types on human thermal comfort. The results indicated high level of thermal discomfort and increased air pollution levels, while a significant correlation between the DI and the AQSI was identified.

Heat waves observed in 2007 in Athens, Greece: synoptic conditions, bioclimatological assessment, air quality levels and health effects.

Heat waves are considered to be increasing in frequency and intensity whereas they comprise a significant weather-related cause of deaths in several countries. Two heat waves occurred in Greece in summer 2007. These severe heat waves are assessed by analyzing the prevailing synoptic conditions, evaluating human thermal discomfort, through the Heat Load Index (HL), as well as investigating its interrelation of air pollutant concentrations, and the daily air quality stress index (AQSI), in the greater region of Athens (Attica), Greece. Furthermore, the relation of HL values and the number of heatstroke and heat exhaustion events recorded in public hospitals operating within the Greek National Health System is examined. Data included radiosonde measurements from the Athens airport station (LGAT), NCEP/NCAR reanalysis data in order to obtain the position of the Subtropical Jet Stream (STJ), GDAS meteorological data for back-trajectory calculation, 10-min meteorological data from 10 Hydro-Meteorological stations and mean hourly values of nitric dioxide (NO(2)), sulphur dioxide (SO(2)) and ozone (O(3)) concentrations, measured at 7 different sites, for the last 10-day period of June and July 2007. Spearman's rank correlation test was used to observe any possible correlation between HL values and air pollutant concentrations, and AQSI values. The results demonstrated different synoptic characteristics for the heat waves of June and July. In the heat wave of June, higher ambient temperatures were recorded and greater HL values were calculated. Extreme discomfort conditions were identified in both heat waves during both day-time and night-time hours. The air pollution analysis showed poor air quality conditions for the heat wave of July, while a significant correlation was found between HL values and average hourly concentrations of O(3), NO(2) and SO(2). The number of heat-affected patients reported during the June heat wave was larger.

Predicting photochemical pollution in an industrial area.

In order to confront pollution events concerning the city of Elefsis, in the environmentally aggravated area of Thriassion Plain, an effort is undertaken to create a model forecasting maximal daily concentrations of NO(x) (NO(2)+NO), NO(2) and O(3). The data analyzed were obtained from the Bureau of Pollution Control and Environments Quality based in Elefsis. The model in question uses hourly values of the pollutants as well as meteorological data recorded at the center of the city of Elefsis from 1993 to 1999. Three fitting methods are utilized, namely ordinary least squares, piecewise, and quantile regression. The verification and reliability of the forecasting models are based on the measurements of the year 2000. The results are considered to be satisfactory, with the forecasted values following the general tendencies.

Diagnostic and therapeutic approach to pituitary incidentalomas.

OBJECTIVE: To review data on epidemiology, differential diagnosis, clinical, laboratory, and imaging findings, natural history, and management of incidentally discovered pituitary lesions (pituitary incidentalomas). METHODS: A nonsystematic review was conducted, including articles indexed in Index Medicus that contained reference to incidentally discovered pituitary masses (pituitary incidentalomas). RESULTS: Both autopsy and sensitive neuroimaging studies (including magnetic resonance imaging) suggest that pituitary incidentalomas are common, affecting approximately 10% of the general population. Although typically small (less than 10 mm in greatest diameter) and clinically silent, some pituitary incidentalomas may be hormonally active or cause mass effects by compressing neighboring structures. In addition, a minority of these lesions may grow over time; hence, long-term follow-up is necessary. Therapeutic interventions, including dopamine agonist therapy (in the case of prolactin-secreting adenomas) or transsphenoidal resection, are indicated in the case of pituitary lesions that are hormonally active, cause mass effects, or increase in size. CONCLUSION: Pituitary incidentalomas are common and constitute a heterogeneous group with regard to pathologic features, clinical, laboratory, and imaging characteristics, natural history, and growth potential. Currently available evidence suggests that many hormonally nonfunctioning pituitary incidentalomas causing no mass effects can be safely managed by follow-up surveillance. Nonetheless, more data are needed for further elucidation of the natural history of these lesions and for improvement in accurate and noninvasive diagnosis and in prediction of growth potential of pituitary incidentalomas. Improved understanding of the pathogenesis of this heterogeneous group of lesions may also lead to the development of novel, noninvasive therapeutic agents, rationally designed to interact with well-characterized molecular targets.