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1.
Emerg Infect Dis ; 7(2): 200-4, 2001.
Article in English | MEDLINE | ID: mdl-11294706

ABSTRACT

Ventilator-associated pneumonia (VAP) is pneumonia in patients who have been on mechanical ventilation for > or =48 hours. VAP is most accurately diagnosed by quantitative culture and microscopy examination of lower respiratory tract secretions, which are best obtained by bronchoscopically directed techniques such as the protected specimen brush and bronchoalveolar lavage. These techniques have acceptable repeatability, and interpretation of results is unaffected by antibiotics administered concurrently for infection at extrapulmonary sites as long as antimicrobial therapy has not been changed for <72 hours before bronchoscopy.


Subject(s)
Pneumonia/diagnosis , Ventilators, Mechanical/adverse effects , Anti-Bacterial Agents/therapeutic use , Bronchoalveolar Lavage/methods , Bronchoscopy/methods , Humans , Pneumonia/etiology , Reproducibility of Results , Respiratory Distress Syndrome/complications , Respiratory System/metabolism
2.
J Clin Microbiol ; 38(11): 4058-65, 2000 Nov.
Article in English | MEDLINE | ID: mdl-11060068

ABSTRACT

Fluorescence-based amplified fragment length polymorphism (fbAFLP) is a novel assay based on the fluorescent analysis of an amplified subset of restriction fragments. The fbAFLP assay involves the selective PCR amplification of restriction fragments from a total digest of genomic DNA. The ligation of adapters with primer-specific sites coupled with primers containing selective nucleotides allowed the full potential of PCR to be realized while maintaining the advantages of restriction endonuclease analysis. Fluorescence-based fragment analysis with polyacrylamide gel electrophoresis provides the accurate band sizing required for homology assessment. The large number of phylogenetically informative characters obtained by fbAFLP is well suited for cluster analysis and database development. The method demonstrated excellent reproducibility and ease of performance and interpretation. We typed 30 epidemiologically well-characterized isolates of vancomycin-resistant enterococci from an outbreak in a university hospital by fbAFLP. Clustering of fbAFLP data matched epidemiological, microbiological, and pulsed-field gel electrophoresis data. This study demonstrates the unprecedented utility of fbAFLP for epidemiological investigation. Future developments in standardization and automation will set fbAFLP as the "gold standard" for molecular typing in epidemiology.


Subject(s)
Enterococcus faecium/classification , Enterococcus faecium/drug effects , Gram-Positive Bacterial Infections/microbiology , Polymorphism, Restriction Fragment Length , Vancomycin Resistance , Bacterial Typing Techniques , Electrophoresis, Gel, Pulsed-Field , Enterococcus faecium/genetics , Enterococcus faecium/isolation & purification , Gram-Positive Bacterial Infections/epidemiology , Hospitals , Humans , Microbial Sensitivity Tests , Reproducibility of Results , Sensitivity and Specificity
3.
Infect Control Hosp Epidemiol ; 21(10): 659-73, 2000 Oct.
Article in English | MEDLINE | ID: mdl-11083185

ABSTRACT

Gene therapy is being studied for the treatment of a variety of acquired and inherited disorders. Retroviruses, adenoviruses, poxviruses, adeno-associated viruses, herpesviruses, and others are being engineered to transfer genes into humans. Treatment protocols using recombinant viruses are being introduced into clinical settings. Infection control professionals will be involved in reviewing the safety of these agents in their clinics and hospitals. To date, only a limited number of articles have been written on infection control in gene therapy, and no widely available recommendations exist from federal or private organizations to guide infection control professionals. The goals of the conference were to provide a forum where gene therapy experts could share their perspectives and experience with infection control in gene therapy and to provide an opportunity for newcomers to the field to learn about issues specific to infection control in gene therapy. Recommendations for infection control in gene therapy were proposed.


Subject(s)
Genetic Therapy , Infection Control , Virus Diseases/therapy , Congresses as Topic , Female , Genetic Therapy/adverse effects , Genetic Therapy/methods , Genetic Therapy/trends , Guidelines as Topic , Humans , Infection Control/methods , Infection Control/standards , United States , United States Food and Drug Administration
4.
Clin Microbiol Rev ; 13(4): 686-707, 2000 Oct.
Article in English | MEDLINE | ID: mdl-11023964

ABSTRACT

After they were first identified in the mid-1980s, vancomycin-resistant enterococci (VRE) spread rapidly and became a major problem in many institutions both in Europe and the United States. Since VRE have intrinsic resistance to most of the commonly used antibiotics and the ability to acquire resistance to most of the current available antibiotics, either by mutation or by receipt of foreign genetic material, they have a selective advantage over other microorganisms in the intestinal flora and pose a major therapeutic challenge. The possibility of transfer of vancomycin resistance genes to other gram-positive organisms raises significant concerns about the emergence of vancomycin-resistant Staphylococcus aureus. We review VRE, including their history, mechanisms of resistance, epidemiology, control measures, and treatment.


Subject(s)
Enterococcus/drug effects , Gram-Positive Bacterial Infections/epidemiology , Gram-Positive Bacterial Infections/microbiology , Vancomycin Resistance , Anti-Bacterial Agents/pharmacology , Anti-Bacterial Agents/therapeutic use , Drug Resistance, Multiple , Gram-Positive Bacterial Infections/drug therapy , Gram-Positive Bacterial Infections/prevention & control , Humans
5.
Infect Control Hosp Epidemiol ; 21(9): 575-82, 2000 Sep.
Article in English | MEDLINE | ID: mdl-11001260

ABSTRACT

OBJECTIVE: To investigate and control an outbreak of colonization and infection caused by vancomycin-resistant enterococci (VRE) in a burn intensive care unit (BICU). DESIGN: Epidemiological investigation, including multiple point-prevalence culture surveys of patients and environment, cultures from hands of healthcare workers (HCWs), pulsed-field gel electrophoresis (PFGE) typing of patient and environmental isolates, case-control study, and institution and monitoring of control measures. SETTING: BICU in an 800-bed university medical center in Galveston, Texas. RESULTS: Between June 6, 1996, and July 14, 1997, 21 patients were colonized by VRE, and 4 of these patients developed bacteremia. Of 2,844 environmental cultures, 338 (11.9%) were positive, but all hand cultures from HCWs were negative. PFGE typing indicated that the outbreak was clonal, with VRE isolates from patients differing by < or =4 bands from the index case. Thirteen of 14 environmental isolates varied by < or =4 bands from the pattern of the index case. A case-control study analyzed by exact logistic regression identified diarrhea (odds ratio [OR], 43.9; 95% confidence interval [CI95], 5.5-infinity; P=.0001) and administration of an antacid (OR, 24.2; CI95, 2.9-infinity; P=.002) as independent risk factors for acquisition of VRE. During a 5-week period in October and November 1996, all patient and 317 environmental cultures were negative for VRE. The outbreak recurred from a contaminated electrocardiogram lead that had not been identified during the prior 5 weeks. VRE were finally eradicated from the BICU in July 1997, using barrier isolation and a very aggressive environmental decontamination program. CONCLUSIONS: A VRE outbreak in a BICU over 13 months was caused by a single clone. After apparent eradication of VRE from a BICU, recrudescence of the outbreak occurred, evidently from a small inapparent source of environmental contamination. Changes in gastrointestinal (GI) tract function (motility) and administration of medications, other than antibiotics, that have an effect on the GI tract may increase the risk of GI tract colonization by VRE in burn patients. Application of barrier isolation and an aggressive environmental decontamination program can eradicate VRE from a burn population.


Subject(s)
Burn Units , Cross Infection/epidemiology , Disease Outbreaks , Enterococcus/drug effects , Gram-Positive Bacterial Infections/epidemiology , Vancomycin Resistance , Adult , Aged , Case-Control Studies , Diarrhea/etiology , Diarrhea/microbiology , Electrocardiography/instrumentation , Enterococcus/pathogenicity , Equipment Contamination , Female , Hospital Bed Capacity, 500 and over , Humans , Infection Control/methods , Male , Middle Aged
6.
Infect Control Hosp Epidemiol ; 21(12): 775-9, 2000 Dec.
Article in English | MEDLINE | ID: mdl-11140913

ABSTRACT

OBJECTIVE: To establish an efficient and sensitive technique for recovering vancomycin-resistant enterococci (VRE) from perianal and environmental samples collected during implementation of control measures for an outbreak of VRE. DESIGN: Perianal and environmental samples were collected in triplicate on sterile swabs. One swab was used to inoculate a selective broth medium containing 6 pg of vancomycin and 8 pg of ciprofloxacin per mL, one to inoculate Campylobacter agar containing 10 microg/mL of vancomycin, and one to inoculate Enterococcosel agar containing 8 microg/mL of vancomycin. SETTING: Samples were collected in the intensive care units of a 600-bed university hospital over a period of 2 months. SAMPLE SELECTION: Patients and their immediate environment were sampled if they resided in a ward with a patient known to be colonized or infected with VRE. RESULTS: Of the 88 perianal samples obtained from 63 patients, 37 were positive for VRE by broth culture, with 36 also recovered on both types of solid media (sensitivity, 97.3%; negative predictive value, 98.1%). Of the initial samples collected from each of the 63 patients, 20 were positive for VRE by all methods. Of the 500 environmental samples cultured, 139 were positive for VRE in broth, with only 33 recovered on Campylobacter agar (sensitivity, 23.7%; negative predictive value, 77.2%) and 22 on Enterococcosel agar (sensitivity, 15.8%; negative predictive value, 75.2%). CONCLUSIONS: Our data indicate that, when performing surveillance cultures during an outbreak of VRE, use of an enrichment broth medium is required to recover VRE contaminating environmental surfaces; however, direct inoculation to selective solid medium is adequate to recover VRE in patient perianal specimens.


Subject(s)
Anal Canal/microbiology , Enterococcus/isolation & purification , Gram-Positive Bacterial Infections/diagnosis , Vancomycin Resistance , Agar , Colony Count, Microbial , Cross Infection , Disease Outbreaks , Enterococcus/drug effects , Humans , Infection Control , Sensitivity and Specificity , Specimen Handling
8.
Arch Pathol Lab Med ; 123(7): 622-5, 1999 Jul.
Article in English | MEDLINE | ID: mdl-10388920

ABSTRACT

OBJECTIVE: To compare the ability of the Vitek GPS-TB card with disk diffusion testing for determining the susceptibility of enterococci to vancomycin. DESIGN: Vitek susceptibility testing was performed using the GPS-TB card and software version R05.03. Disk diffusion susceptibility testing was performed according to National Committee for Clinical Laboratory Standards guidelines. When discrepancies occurred between the interpretation of Vitek and disk diffusion, both tests were repeated and the epsilometer test (E test) and agar screen containing 6 microgram/mL vancomycin were performed. RESULTS: Of 415 isolates tested, 313 were susceptible to vancomycin and 97 were resistant to vancomycin by both test methods. Two isolates were intermediate by Vitek and resistant by disk diffusion, 2 were intermediate by Vitek and susceptible by disk diffusion, and 1 was susceptible by Vitek and intermediate by disk diffusion. All but 1 of these latter 5 isolates (intermediate by Vitek and susceptible by disk diffusion) were available for retesting. On repeat testing, the 2 isolates that were intermediate by Vitek and resistant by disk diffusion were resistant by both methods, the 1 isolate that was intermediate by Vitek and susceptible by disk diffusion was susceptible by both methods, and the isolate that was susceptible by Vitek and intermediate by disk diffusion was also susceptible by both methods. These results were confirmed by E test and agar screen. CONCLUSION: We found the results of the GPS-TB card compared well with disk diffusion. However, isolates with intermediate results by Vitek should be retested using another method, such as the E test.


Subject(s)
Anti-Bacterial Agents/pharmacology , Enterococcus/drug effects , Microbial Sensitivity Tests/methods , Vancomycin/pharmacology , Diffusion , Humans
9.
Infect Control Hosp Epidemiol ; 20(1): 26-30, 1999 Jan.
Article in English | MEDLINE | ID: mdl-9927262

ABSTRACT

OBJECTIVE: To determine the risk factors for colonization or infection with methicillin-resistant Staphylococcus aureus in human immunodeficiency virus (HIV)-infected patients. DESIGN: Retrospective matched-pair case-control study. SETTING: Continuity clinic and inpatient HIV service of a university medical center. POPULATION: Patients with HIV infection from the general population of eastern and coastal Texas and from the Texas Department of Criminal Justice. DATA COLLECTION: Patient charts and the AIDS Care and Clinical Research Program Database were reviewed for the following: age, race, number of admissions, total hospital days, presence of a central venous catheter, serum albumin, total white blood cell count and absolute neutrophil count, invasive or surgical procedures, any cultures positive for S. aureus, and a history of opportunistic illnesses, diabetes, or dermatologic diagnoses. Data also were collected on the administration of antibiotics, antiretroviral therapy, steroids, cancer chemotherapy, and subcutaneous medications. RESULTS: In the univariate analysis, the presence of a central venous catheter, an underlying dermatologic disease, lower serum albumin, prior steroid therapy, and prior antibiotic therapy, particularly antistaphylococcal therapy or multiple courses of antibiotics, were associated with increased risk for colonization or infection with methicillin-resistant S. aureus. Multivariate analysis yielded a model that included presence of a central venous catheter, underlying dermatologic disease, broad-spectrum antibiotic exposure, and number of hospital days as independent risk factors for colonization or infection with methicillin-resistant S. aureus. CONCLUSIONS: In our HIV-infected patient population, prior hospitalization, exposure to broad-spectrum antibiotics, presence of a central venous catheter, and dermatologic disease were risk factors for acquisition of methicillin-resistant S. aureus.


Subject(s)
AIDS-Related Opportunistic Infections/epidemiology , Methicillin Resistance , Staphylococcal Infections/epidemiology , Staphylococcus aureus/pathogenicity , Anti-Bacterial Agents/therapeutic use , Case-Control Studies , Catheterization, Central Venous/adverse effects , Humans , Retrospective Studies , Risk Assessment , Skin Diseases/complications , Staphylococcal Infections/drug therapy , Staphylococcus aureus/drug effects
10.
Am J Respir Crit Care Med ; 158(6): 1839-47, 1998 Dec.
Article in English | MEDLINE | ID: mdl-9847276

ABSTRACT

We performed a prospective observational cohort study of the epidemiology and etiology of nosocomial pneumonia in 358 medical ICU patients in two university-affiliated hospitals. Protected bronchoscopic techniques (protected specimen brush and bronchoalveolar lavage) were used for diagnosis to minimize misclassification. Risk factors for ventilator-associated pneumonia were identified using multiple logistic regression analysis. Twenty-eight cases of pneumonia occurred in 358 patients for a cumulative incidence of 7.8% and incidence rates of 12.5 cases per 1, 000 patient days and 20.5 cases per 1,000 ventilator days. Staphylococcus aureus, Streptococcus pneumoniae, Pseudomonas aeruginosa, and Hemophilus species made up 65% of isolates from the lower respiratory tract, whereas only 12.5% of isolates were enteric gram-negative bacilli. Daily surveillance cultures of the nares, oropharynx, trachea, and stomach demonstrated that tracheal colonization preceded ventilator-associated pneumonia in 93.5%, whereas gastric colonization preceded tracheal colonization for only four of 31 (13%) eventual pathogens. By multiple logistic regression, independent risk factors for ventilator- associated pneumonia were admission serum albumin <= 2.2 g/dl (odds ratio [OR] 5.9; 95% confidence interval [CI] 2.0-17.6; p = 0.0013), maximum positive end-expiratory pressure >= 7.5 cm H2O (OR, 4.6; 95% CI, 1.4 to 15.1; p = 0.012), absence of antibiotic therapy (OR, 6.7; 95% CI, 1.8 to 25.3; p = 0.0054), colonization of the upper respiratory tract by respiratory gram-negative bacilli (OR, 3.4; 95% CI, 1.1 to 10.1; p = 0.028), pack-years of smoking (OR, 2.3 for 50 pack-years; 95% CI, 1. 2 to 4.2; p = 0.012), and duration of mechanical ventilation (OR, 3. 4 for 14 d; 95% CI, 1.5 to 7.8; p = 0.0044). Several of these risk factors for ventilator-associated pneumonia appear amenable to intervention.


Subject(s)
Bronchoscopy , Cross Infection/epidemiology , Pneumonia, Bacterial/epidemiology , Ventilators, Mechanical/adverse effects , Anti-Bacterial Agents/therapeutic use , Bronchoalveolar Lavage , Bronchoscopes , Bronchoscopy/methods , Cohort Studies , Confidence Intervals , Critical Care , Enterobacteriaceae , Enterobacteriaceae Infections/epidemiology , Haemophilus Infections/epidemiology , Humans , Incidence , Logistic Models , Nose/microbiology , Odds Ratio , Oropharynx/microbiology , Pneumonia, Pneumococcal/epidemiology , Pneumonia, Staphylococcal/epidemiology , Positive-Pressure Respiration , Prospective Studies , Pseudomonas Infections/epidemiology , Pseudomonas aeruginosa , Risk Factors , Serum Albumin/analysis , Smoking/epidemiology , Stomach/microbiology , Tennessee/epidemiology , Time Factors , Trachea/microbiology , Ventilators, Mechanical/microbiology
11.
Clin Infect Dis ; 27(3): 463-70, 1998 Sep.
Article in English | MEDLINE | ID: mdl-9770141

ABSTRACT

A prospective observational cohort study of nosocomial sinusitis was carried out in two medical intensive care units. Sinusitis was diagnosed by computed tomographic scanning and the culture of sinus fluid obtained by puncture of a maxillary sinus. Clinical and epidemiological data were collected at the time of admission to the unit and daily thereafter. Specimens from the nares, oropharynx, trachea, and stomach were cultured on admission and daily thereafter. The cumulative incidence of nosocomial sinusitis was 7.7%, and the incidence rates were 12 cases per 1,000 patient-days and 19.8 cases per 1,000 nasoenteric tube-days. Risk factors for nosocomial sinusitis, as determined by multiple logistic regression analysis, included nasal colonization with enteric gram-negative bacilli (odds ratio [OR], 6.4; 95% confidence interval [95% CI], 2.2-18.8; P = .007), feeding via nasoenteric tube (OR, 14.1; 95% CI, 1.7-117.6; P = .015), sedation (OR, 15.9; 95% CI, 1.9-133.5; P = .011), and a Glasgow coma score of < or = 7 (OR, 9.1; 95% CI, 3.0-27.3; P = .0001).


Subject(s)
Cross Infection/epidemiology , Intensive Care Units , Sinusitis/epidemiology , Cohort Studies , Cross Infection/diagnostic imaging , Cross Infection/microbiology , Humans , Incidence , Male , Middle Aged , Paranasal Sinuses/diagnostic imaging , Paranasal Sinuses/microbiology , Prospective Studies , Radiography , Risk Factors , Sinusitis/diagnostic imaging , Sinusitis/microbiology
12.
Infect Dis Clin North Am ; 11(2): 427-57, 1997 Jun.
Article in English | MEDLINE | ID: mdl-9187955

ABSTRACT

Ventilator-associated pneumonia (VAP) is an important complication in patients with respiratory failure who undergo endotracheal intubation and mechanical ventilation. VAP cannot be accurately diagnosed by clinical or radiographic criteria or culture of endotracheal aspirates; however, it can be accurately diagnosed by histopathologic examination of lung tissue, rapid cavitation of a pulmonary infiltrate, culture of empyema fluid, percutaneous lung needle aspiration, simultaneous recovery of the same microorganism from cultures of respiratory secretions, and blood and quantitative culture of lower respiratory tract secretions obtained by bronchoscopy. VAP can be prevented by proper decontamination and use of ventilatory support equipment, practice of proper nursing techniques during care of the mechanically ventilated patient, and use of face mask ventilation in selected patients.


Subject(s)
Cross Infection/diagnosis , Cross Infection/prevention & control , Pneumonia/diagnosis , Pneumonia/prevention & control , Respiration, Artificial/adverse effects , Bronchoalveolar Lavage , Bronchoscopy , Cross Infection/epidemiology , Humans , Infection Control , Intensive Care Units , Pneumonia/epidemiology , Risk Factors
13.
Am J Obstet Gynecol ; 175(2): 304-9; discussion 309-10, 1996 Aug.
Article in English | MEDLINE | ID: mdl-8765246

ABSTRACT

OBJECTIVES: Our purpose was to determine (1) whether risk factors for intraamniotic infection were similar in women delivered of preterm infants versus term infants and (2) whether infection control techniques could decrease the incidence of intrapartum fever on a labor and delivery unit. STUDY DESIGN: A total of 5409 consecutive patients (group 1) admitted to the Medical College of Virginia's labor and delivery unit were followed up prospectively to determine the development of intraamniotic infection. Demographic and intrapartum data were collected by use of a standard data form by infection control practitioners. An additional 2549 consecutive patients (group 2) were followed up after institution of infection-control measures. RESULTS: Intraamniotic infection occurred in 416 of 5399 (7.7%) women (group 1) admitted to the labor and delivery suite. Odds ratios for term and preterm patients having intraamniotic infection with rupture of membranes > or = 12 hours compared with < 12 hours were 5.81 (95% confidence interval 512 to 6.59 and 2.49 (95% confidence interval 1.77 to 3.50), respectively. Odds ratios for term and preterm patients with internal monitors having intraamniotic infection compared with patients in whom internal monitors were not used were 2.01 (95% confidence interval 1.7 to 2.4) and 1.42 (95% confidence interval 0.99 to 2.04), respectively. Odds ratios for term and preterm patients having intraamniotic infection with more than four vaginal examinations compared with four or fewer vaginal examinations was 3.07 (95% confidence interval 2.53-3.73) and 1.59 (95% confidence interval 1.11-2.27), respectively. Intrapartum fever occurred in 475 (8.8%) women in group 1 and in 252 (9.8%) women in group 2 (not significant). CONCLUSIONS: Risk factors (duration of ruptured membranes, use of internal monitoring, number of vaginal examinations) were similar in both term and preterm women with intraamniotic infection. Infection control measures failed to decrease the incidence of intrapartum fever in our patient population.


Subject(s)
Amniotic Fluid/microbiology , Hospitals, Teaching , Hospitals, Urban , Infection Control , Adult , Female , Fetal Membranes, Premature Rupture/complications , Humans , Incidence , Infant, Newborn , Infant, Premature , Infections/epidemiology , Pregnancy , Prospective Studies , Risk Factors , Treatment Failure
16.
Antimicrob Agents Chemother ; 37(6): 1334-42, 1993 Jun.
Article in English | MEDLINE | ID: mdl-8328783

ABSTRACT

Methicillin-resistant Staphylococcus aureus (MRSA) is a major pathogen in hospitals. Current antimicrobial regimens for eradicating colonizing strains are not well defined and are often complicated by the emergence of resistance. The combination of novobiocin plus rifampin in vitro and in vivo was found to prevent the emergence of resistant populations of initially susceptible strains of MRSA, particularly resistance to rifampin. We therefore studied, in a randomized, double-blind, multicenter comparative trial, the combination of novobiocin plus rifampin versus trimethoprim-sulfamethoxazole (T/S) plus rifampin in order to determine the efficacy of each regimen in eradicating MRSA colonization and to further characterize the host factors involved in the response to this antimicrobial therapy. Among the 126 individuals enrolled in the study, 94 (80 patients; 14 hospital personnel) were evaluable. Among the 94 evaluable subjects, no significant demographic or medical differences existed between the two treatment groups. Successful clearance of the colonizing MRSA strains was achieved in 30 of 45 (67%) subjects receiving novobiocin plus rifampin, whereas successful clearance was achieved in 26 of 49 (53%) subjects treated with T/S plus rifampin (P = 0.18). The emergence of resistance to rifampin developed more frequently in 14% (7 of 49) of subjects treated with T/S plus rifampin than in 2% (1 of 45) of subjects treated with novobiocin plus rifampin (P = 0.04). Restriction endonuclease studies of large plasmid DNA demonstrated that the same strain was present at pretherapy and posttherapy in most refractory cases (24 of 29 [83%] subjects). Among the 56 successfully treated subjects, clearance of MRSA was age dependent: 29 of 36 (80%) subjects in the 18- to 49-year-old age group, 19 of 35 (54%) subjects in the 50- to 69-year-old age group, and 8 of 23 (35%) in the 70- to 94-year-old age group (P < 0.01). Clearance was also site dependent; culture-positive samples from wounds were related to a successful outcome in only 22 (48%) of 46 subjects, whereas culture-positive samples from sites other than wounds (e.g., nares, rectum, and sputum) were associated with a success rate of 34 of 48 (71%) subjects (P = 0.02). Foreign bodies in wounds did not prevent the eradication of MRSA by either regimen. T/S plus rifampin was less effective in clearing both pressure and other wounds, whereas novobiocin plus rifampin was equally effective in clearing both pressure and other wounds. There were no significant differences in toxicity between the two regimens. Thus, the combination of novobiocin plus rifampin, in comparison with T/S plus rifampin, was more effective in preventing the emergence of resistance to rifampin and demonstrated a trend toward greater activity in clearing the MRSA carrier state. The response to either combination depended on host factors, particularly age and the site of MRSA colonization.


Subject(s)
Drug Therapy, Combination/therapeutic use , Methicillin Resistance , Staphylococcal Infections/drug therapy , Staphylococcus aureus , Wound Infection/drug therapy , Adolescent , Adult , Aged , DNA, Neoplasm/analysis , Double-Blind Method , Drug Therapy, Combination/adverse effects , Female , Humans , Male , Middle Aged , Nasal Cavity/microbiology , Novobiocin/adverse effects , Novobiocin/therapeutic use , Outcome Assessment, Health Care , Rifampin/therapeutic use , Trimethoprim, Sulfamethoxazole Drug Combination/adverse effects , Trimethoprim, Sulfamethoxazole Drug Combination/therapeutic use
17.
Infect Control Hosp Epidemiol ; 14(5): 255-9, 1993 May.
Article in English | MEDLINE | ID: mdl-8496578

ABSTRACT

OBJECTIVE: To determine the accuracy with which circulating nurses (CNs) classify surgical procedures by risk of contamination in the operating room. DESIGN: Classification of surgical procedures by CNs was compared with the classification of surgical procedures by a physician observer. SETTING: University-affiliated, tertiary care hospital. METHODS: Circulating nurses used the traditional wound classification system of clean, clean-contaminated, contaminated, and dirty-infected to classify surgical wounds in the operating room. A physician remained in the operating room throughout each of 100 surgical procedures and simultaneously classified surgical wounds without the knowledge of the CNs. RESULTS: Classification of surgical wounds by CNs was compared with classification by the physician observer for 50 cases in general surgery and 50 cases in trauma surgery. Compared with the physician observer, the overall accuracy of classification by CNs was 88% (95% confidence interval [CI] of 81.6% to 94.4%; Kappa statistic, 0.83). Classification of surgical wounds was more difficult in trauma surgery (accuracy of 82%) than in general surgery (accuracy of 94%). Accuracy increased for both services when surgical wounds were classified into just two categories (clean or clean-contaminated versus contaminated or dirty-infected). CONCLUSIONS: Surgical wounds can be classified in the operating room with a high degree of accuracy by CNs. Classification was more difficult in trauma than in general surgery, but classification in trauma surgery improved with feedback to and additional education of CNs. The accuracy of classification by CNs was even higher when classifications were divided into just two categories.


Subject(s)
Infection Control Practitioners/standards , Operating Rooms/standards , Surgical Wound Infection/classification , Hospital Bed Capacity, 300 to 499 , Hospitals, University/standards , Humans , Nurse Anesthetists , Operating Room Nursing , Physicians , Prospective Studies , Reproducibility of Results , Risk Factors , Tennessee , Workforce , Wounds and Injuries/surgery
18.
Infect Control Hosp Epidemiol ; 14(4): 211-5, 1993 Apr.
Article in English | MEDLINE | ID: mdl-8478542

ABSTRACT

OBJECTIVE: To determine the sensitivity and specificity of standard infection control surveillance techniques for the identification of surgical wound infections. DESIGN: Surveillance data collected by three infection control practitioners (ICPs) was compared to surveillance data collected simultaneously by a gold standard observer. SETTING: University-affiliated, tertiary care hospital. METHODS: Using standard infection control surveillance techniques (chart review and discussions with patients' nurses and physicians), ICPs collected surveillance data on patients on the General Surgery and Trauma Surgery Services on days 4 and 7 after surgery and then weekly for 30 days or until patients were discharged from the hospital. Simultaneously, a hospital epidemiologist collected surveillance data and examined each patient's wound daily. RESULTS: Nine hundred twenty-five surgical patients including 537 trauma cases and 388 elective general surgery cases were followed postoperatively. The ICPs identified 67 surgical wound infections, and the hospital epidemiologist identified 80 surgical wound infections for a sensitivity of 83.8% with a 95% confidence interval (CI95) of 75.7% to 91.9%. Specificity was 99.8% with a CI95 of 99% to 100%. The sensitivity was the same for trauma surgery and general surgery, but incisional wound infections were more difficult to identify than deep wound infections. During a second validation period, sensitivity was 92.3% with a CI95 of 62% to 100%. CONCLUSIONS: Standard infection control surveillance techniques have the same sensitivity for detection of surgical wound infections as they do for identification of other nosocomial infections. Accurate data on surgical wound infections can be collected without direct examination of surgical wounds.


Subject(s)
Infection Control/methods , Population Surveillance/methods , Surgical Wound Infection/epidemiology , Hospital Bed Capacity, 300 to 499 , Hospitals, Teaching/statistics & numerical data , Humans , Infection Control Practitioners , Reproducibility of Results , Sensitivity and Specificity , Surgical Wound Infection/classification , Surgical Wound Infection/diagnosis , Tennessee/epidemiology
20.
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