ABSTRACT
Youth who are behaviorally aggressive and victimized by their peers comprise a significant population with specific risks and vulnerabilities relative to substance use. The goals of the current study were to examine the roles that youth aggression and peer victimization play in determining the timing of alcohol and marijuana use initiation and the frequency of use 5-years later in a sample of at-risk, aggressive youth. 360 youth (Mage= 10.17 years; 65% boys, 35% girls; 78.1% African American, 20.3% Caucasian, 1.4% Hispanic, and 0.3% other) recruited for a prevention program for at-risk youth were followed for 5 years (4th - 9th grade). Cox PH regressions were conducted to predict timing of alcohol and marijuana use initiation. Zero-inflated negative binomial regressions were used to predict frequency of alcohol and marijuana use 5 years later. Results showed that peer victimization inferred decreased risk of alcohol use initiation. However, this effect was only observed for youth with relatively moderate, and low levels of aggression. Findings suggest that differences in youth aggression and victimization interact to predict distinct outcomes, suggesting the need for a more comprehensive approach when working with aggressive youth who have experienced peer victimization.
Subject(s)
Adolescent Behavior , Aggression , Crime Victims , Marijuana Use , Peer Group , Underage Drinking , Humans , Female , Male , Aggression/psychology , Longitudinal Studies , Crime Victims/psychology , Crime Victims/statistics & numerical data , Adolescent , Marijuana Use/epidemiology , Marijuana Use/psychology , Child , Adolescent Behavior/psychology , Underage Drinking/psychology , Underage Drinking/statistics & numerical data , Alcohol Drinking/psychology , Alcohol Drinking/epidemiology , Bullying/psychology , Bullying/statistics & numerical data , Problem Behavior/psychologyABSTRACT
We examined cultural specificity in how adolescents' coping strategies in response to peer victimization are associated with adjustment with a sample of 7th-8th graders from the United States (n = 292, 60% female, Mage = 13.6, SD = 0.65) and South Korea (n = 462, 50.2% female, Mage = 13.7, SD = 0.58). Participants read scenarios describing victimization and rated the likelihood of utilizing different coping strategies. US adolescents rated conflict resolution, cognitive distancing, and revenge higher than Korean adolescents, while Korean adolescents endorsed social support seeking more than US adolescents. Social support seeking was positively associated with global self-worth in both countries; however, social support seeking was negatively related to depression and social anxiety only for Korean youth.
Subject(s)
Coping Skills , Crime Victims , Humans , Adolescent , Female , United States/epidemiology , Male , Peer Group , Social Support , Republic of Korea , Crime Victims/psychologyABSTRACT
OBJECTIVE: This study examined the association between youth post-disaster stress responses and co-rumination in conversations with a parent several years after a devastating tornado. METHOD: Adolescents (N = 200) drawn from an ongoing study for aggressive youth (ages 13 to 17; 80% African American) and their parents experienced an EF-4 tornado in 2011 and then provided joint recollections about their tornado experiences approximately 5 years later. Recollections were coded for the four components of co-rumination: rehashing problems, dwelling on negative affect, mutual encouragement of problem talk, and speculating about problems. Parent-rated post-traumatic stress symptoms (PTSS) and youth resting respiratory sinus arrhythmia (RSA) were measured approximately 6-months and 1-year post-tornado, respectively. RESULTS: Results indicated that co-rumination could be identified, and reliably measured, in the tornado conversations. Resting RSA moderated the association between post-disaster PTSS and the co-rumination component dwelling on negative affect, such that youth PTSS was associated with higher levels of dwelling on negative affect but only at lower levels of resting RSA (an index of physiological dysregulation). There was no association between youth PTSS and dwelling on negative affect at high resting RSA (an index of better physiological regulation). Youth PTSS and resting RSA were unrelated to the other three co-rumination components. No gender differences were found. CONCLUSIONS: Results provide preliminary evidence establishing the co-rumination coding scheme in a sample of disaster-exposed parents and adolescents. Results also indicated that PTSS and resting RSA are important youth-level factors that relate to how parents and adolescents discuss their disaster experiences even years post-exposure.
ABSTRACT
OBJECTIVE: Children who have been exposed to a natural disaster in their lifetime comprise a significant population with specific risks and vulnerabilities, particularly for at-risk youth. The goal of the current study was to examine the role that severity of disaster exposure plays in determining the timing of alcohol and marijuana use initiation and the frequency of use four years later in a sample of at-risk, aggressive youth exposed to a devastating tornado. Of further interest was the examination of the moderating effect of temperamental fear and inhibitory control. METHOD: Three hundred and forty-six youth (Mage = 11.33; 65% boys; 77.5% African-Americans, 18.3% Caucasian, 1.7% Hispanic) initially recruited for a prevention program for at-risk youth were followed for 4 years after a tornado. Cox PH regressions were conducted to predict timing of alcohol and marijuana use initiation. Zero-inflated negative binomial regressions were used to predict frequency of alcohol and marijuana use 4 years after the tornado. RESULTS: Disaster exposure severity was predictive of frequency of marijuana use four years after the tornado, but exposure alone was not predictive of initiation. Exposure severity predicted increases in risk for alcohol use initiation only for youth high in fear. Additionally, greater levels of inhibitory control protected youth from earlier alcohol use initiation. CONCLUSIONS: Findings highlight a need to research the initiation and frequency of use for substances individually, while also assessing the needs of youth exposed to natural disasters with both their degree of disaster exposure and specific temperamental characteristics in mind.
ABSTRACT
Although there is cultural variability in how individuals make attributions for their own and others' behaviors, cultural variation in youth's attributions about peer victimization and their relation with internalizing problems has gone unexamined. To address this issue, adolescents from the U.S. (n = 292, 60% female, 79.5% White, Mage = 13.6, SD = 0.65) and Korea (n = 462, 50.2% female, Mage = 13.7, SD = 0.58) reported on their peer victimization, depressive symptoms, social anxiety, self-worth, and rated their attributions to vignettes about peer victimization. Multigroup confirmatory analyses found that Korean and American youth conceptualized characterological self-blame, behavioral self-blame, and externalization of blame similarly. However, Korean youth differentially endorsed each of the three types of attributions, while U.S. adolescents endorsed characterological self-blame and behavioral self-blame at similar levels. Attributions had unique relations with internalizing problems (depression, social anxiety, global self-worth) in each culture. In multigroup SEM analyses, characterological self-blame predicted all internalizing problems for U.S. adolescents, while behavioral self-blame was not uniquely related to internalizing problems. For Korean adolescents, behavioral self-blame significantly predicted all internalizing problems, whereas characterological self-blame predicted global self-worth only. The results suggest that attributions about victimization have different adjustment implications in Korea than in the U.S.
Subject(s)
Bullying , Crime Victims , Adolescent , Female , Humans , Male , Peer Group , Republic of Korea , Social PerceptionABSTRACT
This study examined cultural specificity in how interpretations about peer provocation are associated with revenge goals and aggression. The sample consisted of young adolescents from the United States (369 seventh-graders; 54.7% male; 77.2% identified as White) and from Pakistan (358 seventh-graders; 39.2% male). Participants rated their interpretations and revenge goals in response to six peer provocation vignettes and completed peer nominations of aggressive behavior. Multi-group SEM models indicated cultural specificity in how interpretations were related to revenge goals. Interpretations that a friendship with the provocateur was unlikely were uniquely related to revenge goals for Pakistani adolescents. For U.S adolescents positive interpretations were negatively related to revenge but self-blame interpretations were positively related to vengeance goals. Revenge goals were related to aggression similarly across groups.
ABSTRACT
Although cognitive-behavioral interventions have reduced the risk of substance use, little is known about moderating factors in children with disruptive behaviors. This study examined whether aggressive preadolescents' inhibitory control and intervention engagement moderates the effect of group versus individual delivery on their substance use. Following screening for aggression in 4th grade, 360 children were randomly assigned to receive the Coping Power intervention in either group or individual formats. The sample was primarily African American (78%) and male (65%). Assessments were made of children's self-reported substance use from preintervention through a six-year follow-up after intervention, parent-reported inhibitory control at preintervention, and observed behavioral engagement in the group intervention. Multilevel growth modeling found lower increases in substance use slopes for children with low inhibitory control receiving individual intervention, and for children with higher inhibitory control receiving group intervention. Children with low inhibitory control but who displayed more positive behavioral engagement in the group sessions had slower increases in their substance use than did similar children without positive engagement. Aggressive children's level of inhibitory control can lead to tailoring of group versus individual delivery of intervention. Children's positive behavioral engagement in group sessions is a protective factor for children with low inhibitory control.
ABSTRACT
This study examined whether pre-disaster indicators of sympathetic and parasympathetic activity moderated the relation between degree of disaster exposure from an EF-4 tornado and changes in the externalizing and internalizing behavior problems of children at-risk for aggression. Participants included 188 children in 4th-6th grades (65% male; 78% African American; ages 9-13) and their parents from predominantly low-income households who were participating in a prevention study when the tornado occurred in 2011. Fourth-grade children who exhibited elevated levels of aggressive behavior were recruited in three annual cohorts. Parent-rated externalizing and internalizing problems were assessed prior to the tornado (Wave 1; W1), and at 4-12 months (W2), 16-24 months (W3), 42-28 months (W4) and 56-60 months (W5) post-tornado. Children's pre-tornado Skin Conductance Level (SCL) reactivity and Respiratory Sinus Arrhythmia (RSA) withdrawal were assessed at W1 using SCL and RSA measured during resting baseline and during the first 5 min of the Iowa Gambling Task (IGT). Children and parents reported their exposure to tornado-related trauma and disruptions at Wave 3. Children displayed less reduction in externalizing problems if there had been higher child- or parent-reported tornado exposure and less RSA withdrawal, or if they had lower parent-reported TORTE and less SCL reactivity or lower SCL baseline. Highlighting the importance of children's pre-disaster arousal, higher levels of disaster exposure negatively affected children's level of improvement in externalizing problems when children had less vagal withdrawal, and when tornado exposure disrupted the protective effects of higher SCL reactivity and higher SCL baseline.
Subject(s)
Problem Behavior , Respiratory Sinus Arrhythmia , Tornadoes , Adolescent , Aggression , Autonomic Nervous System , Child , Female , Humans , MaleABSTRACT
OBJECTIVE: Values have been found to predict aggressive behavior in adolescents. Adolescents who endorse self-enhancement values typically exhibit more aggressive behaviors, while adolescents who endorse self-transcendent values are less likely to behave aggressively. The associations between values and aggression are low to moderate, suggesting that other factors might moderate them. The study examined whether these associations were moderated by adolescent popularity, an indication of social power. METHOD: The study included 906 adolescents from three cultures: Brazilians (N = 244), Jewish citizens of Israel (N = 250), and Arabic citizens of Israel (N = 409). Personal values were assessed using the Portrait Values Questionnaire (PVQ). Peer nominations were used to assess direct aggression and popularity. RESULTS: Popularity moderated the associations between values and aggression: while the aggressive behavior of popular adolescents was highly associated with their personal values, the behavior of unpopular adolescents was unrelated to their values. This effect consistently emerged across samples, with specific variations for gender and culture. CONCLUSION: Popularity enables adolescents to act according to their personal values: aggressive behaviors increase or decrease according to personal value priorities. The strength of this effect depends on cultural expectations and gender roles.
Subject(s)
Adolescent Behavior , Aggression , Adolescent , Humans , Peer Group , Psychological Distance , Surveys and QuestionnairesABSTRACT
Bystander intervention on behalf of victims of peer aggression is credited with reducing victimization, yet little is known about how bystanders evaluate their intervention efforts. African-, European-, Mexican-, and Native-American adolescents (N = 266) between 13 and 18 years (Mage = 15.0, 54% female) recounted vengeful and peaceful responses to a peer's victimization. For comparison, they also described acts of personal revenge. Youth's explanations of how they evaluated each action were coded for goals and outcomes. Befitting its moral complexity, self-evaluative rationales for third-party revenge cited more goals than the other two conditions. References to benevolence and lack thereof were more frequent after third-party revenge compared to personal revenge. Concerns that security was compromised and that actions contradicted self-direction were high after both types of revenge. Third-party resolution promoted benevolence, competence, self-direction, and security more than third-party revenge. Epistemic network analyses and thematic excerpts revealed the centrality of benevolence goals in adolescents' self-evaluative thinking. Self-focused and identity-relevant goals were cited in concert with benevolence after third-party intervention.
Subject(s)
Adolescent Behavior , Bullying , Crime Victims , Adolescent , Aggression , Female , Humans , Male , Peer GroupABSTRACT
This study explores the association between caregivers' style of co-reminiscing with their adolescents about an EF4 tornado and youth anxiety symptoms several years following the disaster. Caregiver reward of their children's emotional expression, defined as attending to and validating emotionally salient content, is generally associated with adaptive youth psychosocial outcomes. However, caregiver reward of youth recollections that are centered around the youth's negative emotional expression could be an indicator that both caregivers and adolescents are engaged in co-rumination regarding negative emotional experiences. This process may contribute to relatively higher levels of anxiety over time. Adolescents (N = 169) drawn from an ongoing study for aggressive youth (ages 12 to 17; 82% African American) provided individual recollections about their experiences during a devastating tornado 4 to 5 years following the disaster. Caregivers and youth then co-reminisced about their tornado-related experiences. Individual youth recollections were coded for negative personal impact and use of negative emotion words; caregiver-adolescent conversations were coded for caregiver reward of negative emotional expression. Youth who noted more negative personal impacts and used more negative emotion words were higher in parent-rated youth anxiety, and these associations were moderated by caregiver reward of negative emotional expression. The associations between youth recollection qualities and anxiety emerged only when caregivers exhibited high levels of reward of negative emotional expression. These patterns were generally stronger for girls compared to boys. Findings suggest that excessively discussing and rehashing negative experiences, especially several years after the disaster, may be a risk factor for anxiety among disaster-exposed adolescents. (PsycInfo Database Record (c) 2020 APA, all rights reserved).
Subject(s)
Adolescent Behavior/psychology , Anxiety/psychology , Memory, Episodic , Parent-Child Relations , Parents/psychology , Tornadoes , Adolescent , Black or African American/psychology , Child , Female , Humans , Longitudinal Studies , MaleABSTRACT
INTRODUCTION: The Utrecht-Management of Identity Commitments Scale (U-MICS; Crocetti, Rubini, & Meeus, 2008) is a self-report scale that can be applied to measure three identity processes (commitment, in-depth exploration, and reconsideration of commitment) in specific domains. The current study was aimed at testing the applicability of the U-MICS in two new languages: Arabic and Hebrew. Specifically, we (a) tested the fit of the three-factor structure; (b) examined measurement invariance of the Arabic and Hebrew versions; and (c) investigated convergent validity by relating the identity processes (commitment, in-depth exploration, and reconsideration of commitment) to multiple indicators of psychosocial adjustment (self-esteem, prosocial behaviors, and delinquency). METHODS: Participants were 314 Arab and 386 Jewish adolescents living in Israel (N = 770; 54.7% girls; Mage = 13.79, SDage = 0.51, age-range = 12-15). Assessments of identity processes, self-esteem, and delinquency were collected using self-report measures, while prosocial behaviors were measured through peer nominations. RESULTS: The results highlighted that (a) the three-factor model fit the data excellently in the total sample as well as in Arab and Jewish samples; (b) hierarchical levels of measurement invariance (configural, metric, and scalar), as well as invariance of covariances, could be clearly established; and (c) convergent validity was demonstrated by showing meaningful associations between identity processes and indicators of psychosocial functioning. CONCLUSIONS: This study indicates that the U-MICS is a reliable instrument for assessing identity processes in Arabic and Hebrew-speaking adolescents.
Subject(s)
Self Concept , Self Report/standards , Social Behavior , Adolescent , Arabs/psychology , Arabs/statistics & numerical data , Female , Humans , Israel , Jews/psychology , Jews/statistics & numerical data , Juvenile Delinquency/psychology , Language , Male , Psychology, Adolescent , TranslationsABSTRACT
The primary objective was to determine if Angus bull fertility varied by number of sperm inseminated. A secondary objective was to characterize the potential impact of random variation on fertility using two identical sperm per dose treatments, which differed only in straw color. Computer-assisted sperm analysis (CASA) and flow cytometry (FC) were used to identify post-thaw sperm characteristics associated with field fertility differences between bulls. Ejaculates from five Angus bulls were collected, extended, and cryopreserved at 10, 20, 20 or 40 × 106 sperm per dose in color-coded 0.5-mL French straws. Multiparous cows (n = 4866) from ten Brazilian farms were synchronized for first-service timed artificial insemination (TAI). Bull identification and straw color were recorded at TAI. Pregnancy per TAI (P/TAI) did not differ between sperm doses (43.8, 45.3, 43.8 and 47.1% for 10, 20, 20 or 40 × 106 sperm respectively; P = 0.31) nor was there an interaction between bull and dose (P = 0.53). The P/TAI differed between bulls and ranged from 40.7 to 48.1% (P < 0.01). The overall P/TAI between the two control groups were not different (45.3 vs 43.8%); however, the numerical variation within bull ranged from 0.5 to 4.9 percentage points. Numerous CASA and FC post-thaw sperm characteristics differed among bulls (P < 0.05), but these characteristics did not explain the fertility difference between bulls. Principal component analysis provided a multivariate description of the CASA and FC data, where three principal components (Prin1, Prin2, and Prin3) accounted for a combined total of 88.7% of the data variability. The primary components of each PCA axis were flow cytometric measures of sperm viability and DNA fragmentation (Prin1), and CASA-derived sperm movement patterns (Prin2) and motility (Prin3); however, the relative influence of these characteristics varied by bull. Although fertility differences between bulls were detected, neither sperm per dose nor post-thaw in vitro sperm analyses (CASA and FC) were able to explain the observed differences in field fertility between bulls, further illustrating the difficulties in predicting bull fertility.
Subject(s)
Cattle , Insemination, Artificial/veterinary , Spermatozoa/physiology , Animals , Female , Fertility , Male , Pregnancy , Semen Preservation/veterinary , Sperm MotilityABSTRACT
Although disaster-related posttraumatic stress symptoms (PTSS) typically decrease in intensity over time, some youth continue to report elevated levels of PTSS many years after the disaster. The current study examines two processes that may help to explain the link between disaster exposure and enduring PTSS: caregiver emotion socialization and youth recollection qualities. One hundred and twenty-two youth (ages 12 to 17) and their female caregivers who experienced an EF-4 tornado co-reminisced about the event, and adolescents provided independent recollections between 3 and 4 years after the tornado. Adolescent individual transcripts were coded for coherence and negative personal impact, qualities that have been found to contribute to meaning making. Parent-adolescent conversations were coded for caregiver egocentrism, a construct derived from the emotion socialization literature to reflect the extent to which the caregiver centered the conversation on her own emotions and experiences. Egocentrism predicted higher youth PTSS, and this association was mediated by the coherence of adolescents' narratives. The association between coherence and PTSS was stronger for youth who focused more on the negative personal impacts of the tornado event during their recollections. Results suggest that enduring tornado-related PTSS may be influenced in part by the interplay of caregiver emotion socialization practices and youth recollection qualities.
Subject(s)
Caregivers/psychology , Disasters , Mental Recall , Stress Disorders, Post-Traumatic/psychology , Tornadoes , Adolescent , Child , Emotions/physiology , Female , Humans , Male , Parent-Child Relations , Parents/psychologyABSTRACT
In this preliminary study, we examined peer victimization in adolescents with Down syndrome (DS) and how it relates to language and communication skills. We modified the Childrens' Social Experience Questionnaire (Crick and Grotpeter in Dev Psychopathol 8:367-380, 1996) to better suit adolescents with DS by simplifying vocabulary and syntax, using two step interview response format. Internal reliability was adequate, and all peer victimization measures were significantly elevated compared to a typically developing sample. Further, peer victimization (especially relational victimization) correlated with speech intelligibility, pragmatic judgment, conversational behavior, and receptive vocabulary. These preliminary data suggest that having DS may put adolescents at risk for peer victimization, but having relatively good language/communication skills may be a protective factor. Further research is warranted on this topic.
Subject(s)
Bullying/psychology , Crime Victims/psychology , Down Syndrome/psychology , Language Development Disorders/psychology , Adolescent , Bullying/statistics & numerical data , Child , Child Language , Communication , Crime Victims/statistics & numerical data , Female , Humans , Male , Peer Group , Reproducibility of Results , Social Behavior , Speech Intelligibility , Surveys and Questionnaires , VocabularyABSTRACT
OBJECTIVE: The current study examined how severity of disaster exposure and predisaster individual and family characteristics predicted trajectories of disaster-related posttraumatic stress symptoms (PTSS) in children over 4 years following a devastating EF-4 tornado. METHOD: Participants (n = 346; 65% male; 77.5% African American) were 4th-6th-graders and their caregivers, from predominantly low-income households, who were already participating in a longitudinal study of indicated prevention effects for externalizing outcomes when the tornado occurred in 2011. Latent class trajectory analyses were used to identify disaster-related PTSS trajectory groups across the 4-year postdisaster period. RESULTS: Three groups were identified: (1) a group that declined (recovery) in PTSS over time (15.90%); (2) a group that was stable and low in PTSS over time (76.87%); and (3) a group that was stable and high (chronic) in PTSS over time (7.23%). Multinomial logistic regression analyses revealed that greater tornado exposure predicted membership in the declining trajectory group relative to the low-stable group. Positive parenting and pretornado caregiver trauma exposure also moderated how disaster exposure, particularly perceived life threat, predicted PTSS trajectories. CONCLUSIONS: Some youth reported elevated disaster-related PTSS repeatedly for 4 years following a devastating tornado. Consistent with the concept of equifinality, results suggest that there are several pre-exposure risk factors that may increase risk for a chronic PTSS trajectory following disaster exposure. (PsycINFO Database Record (c) 2019 APA, all rights reserved).
Subject(s)
Family/psychology , Parenting/psychology , Poverty/psychology , Stress Disorders, Post-Traumatic/etiology , Stress Disorders, Post-Traumatic/psychology , Tornadoes , Child , Disasters , Female , Humans , Longitudinal Studies , Male , Risk FactorsABSTRACT
In order to better understand why some children retaliate when they feel provoked and others do not, the present study identified "pacifistically-oriented" children who made negative interpretations in response to unambiguous provocations, yet did not endorse revenge goals, and compared them to "revenge-seeking" children who also made negative interpretations but did endorse revenge goals. Groups were identified based on seventh graders' (N = 367; 54.77% male; 22.89% racial/ethnic minority) responses to hypothetical situations in which a peer excluded and insulted them. Comparing these groups revealed that Pacifists endorsed relationship-maintaining goals and emotion regulation goals more highly than Revenge-Seekers. Revenge-Seekers reported more anger and endorsed beliefs about negative reciprocity and aggression being legitimate more highly than Pacifists. Additionally, Revenge-Seekers were more disrespect sensitive than were Pacifists, based on a measure of vigilance for signs of disrespect and expectations that others would disrespect them. Together these findings point to social-cognitive and emotion-related processes that may inhibit revenge-seeking in unambiguous provocation situations, even when children interpret the peer's behavior quite negatively.
Subject(s)
Aggression/psychology , Child Behavior/psychology , Inhibition, Psychological , Peer Group , Violence/psychology , Anger , Child , Conflict, Psychological , Female , Humans , Male , Social BehaviorABSTRACT
Although research has examined how values are correlated with behavior, little has examined how the system of values predicts behavior. In a cross-cultural sample of American (109 European American; 216 African American) and Israeli (318 Arab Israeli; 216 Jewish Israeli) adolescents, the present study used latent profile analysis to identify groups which reflected the theoretical structure of values across both cultures. Four profiles were found: self-focused, anxiety-free, other-focused, and undifferentiated. Results indicated that Self-Focused adolescents were the most aggressive and viewed as leaders by their peers compared to the other groups. Self-Focused and anxiety-free youth reported more delinquency than their peers. Few differences between cultural groups emerged, suggesting that this approach is a promising avenue for understanding heterogeneity in behavior.
Subject(s)
Adolescent Behavior/ethnology , Personality Assessment , Social Values/ethnology , Adolescent , Adolescent Behavior/psychology , Cross-Cultural Comparison , Cross-Sectional Studies , Female , Humans , Israel/ethnology , Male , Models, Theoretical , Peer Group , Social Behavior , United States/ethnologyABSTRACT
Mycoplasma synoviae (MS) is a poultry pathogen that has had an increasing incidence and economic impact over the past few years. Strain identification is necessary for outbreak investigation, infection source identification, and facilitating prevention and control as well as eradication efforts. Currently, a segment of the variable lipoprotein hemagglutinin A (vlhA) gene (420 bp) is the only target that is used for MS strain identification. A major limitation of this assay is that colonality of typed samples can only be inferred if their vlhA sequences are identical; however, if their sequences are different, the degree of relatedness is uncertain. In this study we propose a multilocus sequence typing (MLST) assay to further refine MS strain identification. After initial screening of 24 housekeeping genes as potential targets, seven genes were selected for the MLST assay. An internal segment (450-711 bp) from each of the seven genes was successfully amplified and sequenced from 58 different MS strains and field isolates (n = 30) or positive clinical samples (n = 28). The collective sequence of all seven gene segments (3960 bp total) was used for MS sequence typing. The 58 tested MS samples were typed into 30 different sequence types using the MLST assay and, coincidentally, all the samples were typed into 30 sequence types using the vlhA assay. However, the phylogenetic tree generated using the MLST data was more congruent to the epidemiologic information than was the tree generated by the vlhA assay. We suggest that the newly developed MLST assay and the vlhA assay could be used in tandem for MS typing. The MLST assay will be a valuable and more reliable tool for MS sequence typing, providing better understanding of the epidemiology of MS infection. This in turn will aid disease prevention, control, and eradication efforts.
Subject(s)
Multilocus Sequence Typing/methods , Mycoplasma Infections/veterinary , Mycoplasma synoviae/isolation & purification , Poultry Diseases/microbiology , Animals , Bacterial Proteins/genetics , Chickens , Genotype , Mycoplasma Infections/microbiology , Mycoplasma synoviae/classification , Mycoplasma synoviae/genetics , Mycoplasma synoviae/physiology , PhylogenyABSTRACT
Social functioning is critical for the successful navigation of everyday life for children, adolescents, and adults. Recent theories have postulated a neuropsychological basis for social functioning with particularly strong links with the executive functioning (EF) system. The current study examined attention problems as a mediator between EF (e.g., working memory, planning, and response inhibition) and social functioning in a child and adolescent outpatient sample. Participants were 218 children ages 6-16 (M = 10.23; SD = 2.52; 68.8 % males) who were referred to an outpatient clinic for psychoeducational assessment. Bias-corrected bootstrapping mediation analyses were used to examine the hypothesized models. The effects of working memory and planning (but not response inhibition) on social problems were mediated by attention problems in both teacher- and mother-reported models. These findings also held up in cross-source models (e.g., mother-reported attention problems as a mediator in a model predicting teacher-reported social problems). These findings have implications for dimensional models of social functioning and conceptual models for specific clinical populations (e.g., attention-deficit/hyperactivity disorder).