ABSTRACT
AT-rich interacting domain (ARID)-containing proteins, Arids, are a heterogeneous DNA-binding protein family involved in transcription regulation and chromatin processing. For the member Arid5a, no exact DNA-binding preference has been experimentally defined so far. Additionally, the protein binds to mRNA motifs for transcript stabilization, supposedly through the DNA-binding ARID domain. To date, however, no unbiased RNA motif definition and clear dissection of nucleic acid-binding through the ARID domain have been undertaken. Using NMR-centered biochemistry, we here define the Arid5a DNA preference. Further, high-throughput in vitro binding reveals a consensus RNA-binding motif engaged by the core ARID domain. Finally, transcriptome-wide binding (iCLIP2) reveals that Arid5a has a weak preference for (A)U-rich regions in pre-mRNA transcripts of factors related to RNA processing. We find that the intrinsically disordered regions flanking the ARID domain modulate the specificity and affinity of DNA binding, while they appear crucial for RNA interactions. Ultimately, our data suggest that Arid5a uses its extended ARID domain for bifunctional gene regulation and that the involvement of IDR extensions is a more general feature of Arids in interacting with different nucleic acids at the chromatin-mRNA interface.
Subject(s)
DNA-Binding Proteins , DNA , Transcription Factors , Humans , DNA-Binding Proteins/metabolism , DNA-Binding Proteins/genetics , DNA-Binding Proteins/chemistry , DNA/metabolism , DNA/chemistry , DNA/genetics , Transcription Factors/metabolism , Transcription Factors/genetics , Transcription Factors/chemistry , Protein Domains , Gene Expression Regulation , Protein Binding , RNA, Messenger/metabolism , RNA, Messenger/genetics , RNA/metabolism , RNA/chemistry , RNA/geneticsABSTRACT
Nuclear RNA binding proteins (RBPs) are difficult to study because they often belong to large protein families and form extensive networks of auto- and crossregulation. They are highly abundant and many localize to condensates with a slow turnover, requiring long depletion times or knockouts that cannot distinguish between direct and indirect or compensatory effects. Here, we developed a system that is optimized for the rapid degradation of nuclear RBPs, called hGRAD. It comes as a "one-fits-all" plasmid, and integration into any cell line with endogenously GFP-tagged proteins allows for an inducible, rapid, and complete knockdown. We show that the nuclear RBPs SRSF3, SRSF5, SRRM2, and NONO are completely cleared from nuclear speckles and paraspeckles within 2 h. hGRAD works in various cell types, is more efficient than previous methods, and does not require the expression of exogenous ubiquitin ligases. Combining SRSF5 hGRAD degradation with Nascent-seq uncovered transient transcript changes, compensatory mechanisms, and an effect of SRSF5 on transcript stability.
Subject(s)
Gene Knockdown Techniques , RNA-Binding Proteins , Cell Line , RNA-Binding Proteins/genetics , Plasmids/genetics , Ubiquitin-Protein LigasesABSTRACT
Hypoxia induces massive changes in alternative splicing (AS) to adapt cells to the lack of oxygen. Here, we identify the splicing factor SRSF6 as a key factor in the AS response to hypoxia. The SRSF6 level is strongly reduced in acute hypoxia, which serves a dual purpose: it allows for exon skipping and triggers the dispersal of nuclear speckles. Our data suggest that cells use dispersal of nuclear speckles to reprogram their gene expression during hypoxic adaptation and that SRSF6 plays an important role in cohesion of nuclear speckles. Down-regulation of SRSF6 is achieved through inclusion of a poison cassette exon (PCE) promoted by SRSF4. Removing the PCE 3' splice site using CRISPR/Cas9 abolishes SRSF6 reduction in hypoxia. Aberrantly high SRSF6 levels in hypoxia attenuate hypoxia-mediated AS and impair dispersal of nuclear speckles. As a consequence, proliferation and genomic instability are increased, while the stress response is suppressed. The SRSF4-PCE-SRSF6 hypoxia axis is active in different cancer types, and high SRSF6 expression in hypoxic tumors correlates with a poor prognosis. We propose that the ultra-conserved PCE of SRSF6 acts as a tumor suppressor and that its inclusion in hypoxia is crucial to reduce SRSF6 levels. This may prevent tumor cells from entering the metastatic route of hypoxia adaptation.
Subject(s)
Cell Hypoxia , Nuclear Speckles , RNA Splicing , Serine-Arginine Splicing Factors , Humans , Alternative Splicing , Exons/genetics , Phosphoproteins/genetics , Serine-Arginine Splicing Factors/genetics , Serine-Arginine Splicing Factors/metabolism , HeLa CellsABSTRACT
Control of posttranscriptional mRNA decay is a crucial determinant of cell homeostasis and differentiation. mRNA lifetime is governed by cis-regulatory elements in their 3' untranslated regions (UTR). Despite ongoing progress in the identification of cis elements we have little knowledge about the functional and structural integration of multiple elements in 3'UTR regulatory hubs and their recognition by mRNA-binding proteins (RBPs). Structural analyses are complicated by inconsistent mapping and prediction of RNA fold, by dynamics, and size. We here, for the first time, provide the secondary structure of a complete mRNA 3'UTR. We use NMR spectroscopy in a divide-and-conquer strategy complemented with SAXS, In-line probing and SHAPE-seq applied to the 3'UTR of Ox40 mRNA, which encodes a T-cell co-receptor repressed by the protein Roquin. We provide contributions of RNA elements to Roquin-binding. The protein uses its extended bi-modal ROQ domain to sequentially engage in a 2:1 stoichiometry with a 3'UTR core motif. We observe differential binding of Roquin to decay elements depending on their structural embedment. Our data underpins the importance of studying RNA regulation in a full sequence and structural context. This study serves as a paradigm for an approach in analysing structured RNA-regulatory hubs and their binding by RBPs.
Subject(s)
3' Untranslated Regions , Nucleic Acid Conformation , Magnetic Resonance Spectroscopy , RNA, Messenger/metabolism , Scattering, Small Angle , X-Ray DiffractionABSTRACT
[Figure: see text].
Subject(s)
Hypertrophy, Left Ventricular/metabolism , RNA, Long Noncoding/genetics , RNA-Binding Proteins/metabolism , Receptors, Glucocorticoid/metabolism , Serine-Arginine Splicing Factors/metabolism , Animals , Cells, Cultured , Hypertrophy, Left Ventricular/genetics , Male , Mice , Mice, Inbred C57BL , Myocytes, Cardiac/metabolism , RNA, Long Noncoding/metabolism , RNA-Binding Proteins/genetics , Receptors, Glucocorticoid/genetics , Serine-Arginine Splicing Factors/genetics , TranscriptomeABSTRACT
BACKGROUND: Alternative polyadenylation (APA) refers to the regulated selection of polyadenylation sites (PASs) in transcripts, which determines the length of their 3' untranslated regions (3'UTRs). We have recently shown that SRSF3 and SRSF7, two closely related SR proteins, connect APA with mRNA export. The mechanism underlying APA regulation by SRSF3 and SRSF7 remained unknown. RESULTS: Here we combine iCLIP and 3'-end sequencing and find that SRSF3 and SRSF7 bind upstream of proximal PASs (pPASs), but they exert opposite effects on 3'UTR length. SRSF7 enhances pPAS usage in a concentration-dependent but splicing-independent manner by recruiting the cleavage factor FIP1, generating short 3'UTRs. Protein domains unique to SRSF7, which are absent from SRSF3, contribute to FIP1 recruitment. In contrast, SRSF3 promotes distal PAS (dPAS) usage and hence long 3'UTRs directly by counteracting SRSF7, but also indirectly by maintaining high levels of cleavage factor Im (CFIm) via alternative splicing. Upon SRSF3 depletion, CFIm levels decrease and 3'UTRs are shortened. The indirect SRSF3 targets are particularly sensitive to low CFIm levels, because here CFIm serves a dual function; it enhances dPAS and inhibits pPAS usage by binding immediately downstream and assembling unproductive cleavage complexes, which together promotes long 3'UTRs. CONCLUSIONS: We demonstrate that SRSF3 and SRSF7 are direct modulators of pPAS usage and show how small differences in the domain architecture of SR proteins can confer opposite effects on pPAS regulation.
Subject(s)
3' Untranslated Regions , Cleavage And Polyadenylation Specificity Factor/genetics , Gene Expression Regulation , Poly A , Serine-Arginine Splicing Factors/metabolism , Alternative Splicing , Animals , Base Sequence , Mice , Models, Biological , Monomeric GTP-Binding Proteins/metabolism , Neurons , Phosphorylation , Poly(A)-Binding Proteins/metabolism , Polyadenylation , Protein Binding , Protein Interaction Domains and Motifs , RNA, Messenger/genetics , RNA, Messenger/metabolismABSTRACT
SRSF7 is an essential RNA-binding protein whose misexpression promotes cancer. Here, we describe how SRSF7 maintains its protein homeostasis in murine P19 cells using an intricate negative feedback mechanism. SRSF7 binding to its premessenger RNA promotes inclusion of a poison cassette exon and transcript degradation via nonsense-mediated decay (NMD). However, elevated SRSF7 levels inhibit NMD and promote translation of two protein halves, termed Split-ORFs, from the bicistronic SRSF7-PCE transcript. The first half acts as dominant-negative isoform suppressing poison cassette exon inclusion and instead promoting the retention of flanking introns containing repeated SRSF7 binding sites. Massive SRSF7 binding to these sites and its oligomerization promote the assembly of large nuclear bodies, which sequester SRSF7 transcripts at their transcription site, preventing their export and restoring normal SRSF7 protein levels. We further show that hundreds of human and mouse NMD targets, especially RNA-binding proteins, encode potential Split-ORFs, some of which are expressed under specific cellular conditions.
Subject(s)
Gene Expression Regulation , Neoplasm Proteins/genetics , Open Reading Frames , RNA Precursors/genetics , RNA-Binding Proteins/genetics , Serine-Arginine Splicing Factors/genetics , Amino Acid Sequence , Animals , Base Sequence , Binding Sites , Cell Line, Tumor , Cell Nucleus/metabolism , Cell Nucleus/ultrastructure , Exons , Homeostasis/genetics , Mice , Mouse Embryonic Stem Cells/cytology , Mouse Embryonic Stem Cells/metabolism , Neoplasm Proteins/metabolism , Nonsense Mediated mRNA Decay , Protein Binding , Protein Biosynthesis , RNA Precursors/metabolism , RNA-Binding Proteins/classification , RNA-Binding Proteins/metabolism , Serine-Arginine Splicing Factors/metabolism , Transcription, GeneticABSTRACT
In mitotic cells, establishment of sister chromatid cohesion requires acetylation of the cohesin subunit SMC3 (acSMC3) by ESCO1 and/or ESCO2. Meiotic cohesin plays additional but poorly understood roles in the formation of chromosome axial elements (AEs) and synaptonemal complexes. Here, we show that levels of ESCO2, acSMC3, and the pro-cohesion factor sororin increase on meiotic chromosomes as homologs synapse. These proteins are less abundant on the largely unsynapsed sex chromosomes, whose sister chromatid cohesion appears weaker throughout the meiotic prophase. Using three distinct conditional Esco2 knockout mouse strains, we demonstrate that ESCO2 is essential for male gametogenesis. Partial depletion of ESCO2 in prophase I spermatocytes delays chromosome synapsis and further weakens cohesion along sex chromosomes, which show extensive separation of AEs into single chromatids. Unsynapsed regions of autosomes are associated with the sex chromatin and also display split AEs. This study provides the first evidence for a specific role of ESCO2 in mammalian meiosis, identifies a particular ESCO2 dependence of sex chromosome cohesion and suggests support of autosomal synapsis by acSMC3-stabilized cohesion.
Subject(s)
Acetyltransferases/metabolism , Chromatids/metabolism , Chromosome Pairing/physiology , Acetylation , Acetyltransferases/genetics , Acetyltransferases/physiology , Animals , Cell Cycle Proteins , Chromatids/genetics , Chromosomal Proteins, Non-Histone , Chromosome Pairing/genetics , Chromosome Segregation/genetics , Chromosome Segregation/physiology , Chromosome Structures/metabolism , Gametogenesis/genetics , Male , Meiosis/genetics , Mice , Mice, Inbred C57BL , Nuclear Proteins/genetics , Sex Chromosomes/metabolism , Spermatocytes/metabolism , Synaptonemal Complex/metabolism , CohesinsABSTRACT
Many proteins appear exclusively nuclear at steady-state but in fact shuttle continuously back and forth between the nucleus and the cytoplasm. For example, nuclear RNA-binding proteins (RBPs) often accompany mRNAs to the cytoplasm, where they can regulate subcellular localization, translation and/or decay of their cargos before shuttling back to the nucleus. Nucleocytoplasmic shuttling must be tightly regulated, as mislocalization of several RBPs with prion-like domains such as FUS and TDP-43 causes the cytoplasmic accumulation of solid pathological aggregates that have been implicated in neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Traditionally, interspecies heterokaryon assays have been used to determine whether a nuclear protein of interest shuttles; those assays are based on the fusion between donor and recipient cells from two different species (e.g., mouse and human), which can be distinguished based on different chromatin staining patterns, and detecting the appearance of the protein in the recipient nucleus. However, identification of heterokaryons requires experience and is prone to error, which makes it difficult to obtain high-quality data for quantitative studies. Moreover, transient overexpression of fluorescently tagged RBPs in donor cells often leads to their aberrant subcellular localization. Here, we present a quantitative assay where stable donor cell lines expressing near-physiological levels of eGFP-tagged RBPs are fused to recipient cells expressing the membrane marker CAAX-mCherry, allowing to readily identify and image a large number of high-confidence heterokaryons. Our assay can be used to measure the shuttling activity of any nuclear protein of interest in different cell types, under different cellular conditions or between mutant proteins.
ABSTRACT
SR proteins function in nuclear pre-mRNA processing, mRNA export, and translation. To investigate their cellular dynamics, we developed a quantitative assay, which detects differences in nucleocytoplasmic shuttling among seven canonical SR protein family members. As expected, SRSF2 and SRSF5 shuttle poorly in HeLa cells but surprisingly display considerable shuttling in pluripotent murine P19 cells. Combining individual-resolution cross-linking and immunoprecipitation (iCLIP) and mass spectrometry, we show that elevated arginine methylation of SRSF5 and lower phosphorylation levels of cobound SRSF2 enhance shuttling of SRSF5 in P19 cells by modulating protein-protein and protein-RNA interactions. Moreover, SRSF5 is bound to pluripotency-specific transcripts such as Lin28a and Pou5f1/Oct4 in the cytoplasm. SRSF5 depletion reduces and overexpression increases their cytoplasmic mRNA levels, suggesting that enhanced mRNA export by SRSF5 is required for the expression of pluripotency factors. Remarkably, neural differentiation of P19 cells leads to dramatically reduced SRSF5 shuttling. Our findings indicate that posttranslational modification of SR proteins underlies the regulation of their mRNA export activities and distinguishes pluripotent from differentiated cells.
Subject(s)
Cell Differentiation , Cell Nucleus/metabolism , Pluripotent Stem Cells/metabolism , RNA, Messenger/metabolism , Serine-Arginine Splicing Factors/metabolism , Active Transport, Cell Nucleus , Animals , Arginine , DNA-Binding Proteins/genetics , DNA-Binding Proteins/metabolism , HeLa Cells , Humans , Immunoprecipitation , Methylation , Mice , Neurogenesis , Phenotype , Phosphorylation , Protein Binding , Protein Processing, Post-Translational , RNA Interference , RNA, Messenger/genetics , RNA-Binding Proteins/genetics , RNA-Binding Proteins/metabolism , Repressor Proteins/genetics , Repressor Proteins/metabolism , Serine-Arginine Splicing Factors/genetics , Tandem Mass Spectrometry , Transcription Factors/genetics , Transcription Factors/metabolism , TransfectionABSTRACT
The cohesin complex is essential for mitosis and meiosis. The specific meiotic roles of individual cohesin proteins are incompletely understood. We report in vivo functions of the only meiosis-specific STAG component of cohesin, STAG3. Newly generated STAG3-deficient mice of both sexes are sterile with meiotic arrest. In these mice, meiotic chromosome architecture is severely disrupted as no bona fide axial elements (AE) form and homologous chromosomes do not synapse. Axial element protein SYCP3 forms dot-like structures, many partially overlapping with centromeres. Asynapsis marker HORMAD1 is diffusely distributed throughout the chromatin, and SYCP1, which normally marks synapsed axes, is largely absent. Centromeric and telomeric sister chromatid cohesion are impaired. Centromere and telomere clustering occurs in the absence of STAG3, and telomere structure is not severely affected. Other cohesin proteins are present, localize throughout the STAG3-devoid chromatin, and form complexes with cohesin SMC1ß. No other deficiency in a single meiosis-specific cohesin causes a phenotype as drastic as STAG3 deficiency. STAG3 emerges as the key STAG cohesin involved in major functions of meiotic cohesin.
Subject(s)
Cell Cycle Proteins/metabolism , Chromatids/physiology , Chromosomal Proteins, Non-Histone/metabolism , Chromosome Segregation/genetics , Meiosis/genetics , Nuclear Proteins/metabolism , Animals , Cell Cycle Proteins/genetics , Centromere/genetics , Centromere/physiology , Chromatids/genetics , Chromosomal Proteins, Non-Histone/genetics , Chromosomes/genetics , Female , Male , Mice , Nuclear Proteins/genetics , Oocytes/cytology , Oocytes/metabolism , Phosphoproteins/genetics , Phosphoproteins/metabolism , Specific Pathogen-Free Organisms , Spermatocytes/cytology , Spermatocytes/metabolism , Synaptonemal Complex/genetics , Synaptonemal Complex/metabolism , Telomere/genetics , Telomere/physiology , Testis/cytology , Testis/metabolism , CohesinsABSTRACT
Sister chromatid cohesion depends on cohesin, a tripartite complex that forms ring structures to hold sister chromatids together in mitosis and meiosis. Meiocytes feature a multiplicity of distinct cohesin proteins and complexes, some meiosis specific, which serve additional functions such as supporting synapsis of two pairs of sister chromatids and determining the loop-axis architecture of prophase I chromosomes. Despite considerable new insights gained in the past few years into the localization and function of some cohesin proteins, and the recent identification of yet another meiosis-specific cohesin subunit, a plethora of open questions remains, which concern not only fundamental germ cell biology but also the consequences of cohesin impairment for human reproductive health.
Subject(s)
Cell Cycle Proteins/metabolism , Chromosomal Proteins, Non-Histone/metabolism , Gametogenesis , Animals , Cell Cycle Checkpoints , Humans , Kinetochores/metabolism , Meiosis , Oocytes/metabolism , CohesinsABSTRACT
Protozoan parasites of the genus Leishmania are found as promastigotes in the sandfly vector and as amastigotes in mammalian macrophages. Mechanisms controlling stage-regulated gene expression in these organisms are poorly understood. Here, we applied a comprehensive approach consisting of protein prefractionation, global proteomics and targeted DNA microarray analysis to the study of stage differentiation in Leishmania. By excluding some abundant structural proteins and reducing complexity, we detected and identified numerous novel differentially expressed protein isoforms in L. infantum. Using 2-D gels, over 2200 protein isoforms were visualized in each developmental stage. Of these, 6.1% were strongly increased or appeared unique in the promastigote stage, while the relative amounts of 12.4% were increased in amastigotes. Amastigote-specific protein isoform and mRNA expression trends correlated modestly (53%), while no correlation was found for promastigote-specific spots. Even where direction of regulation was similar, fold-changes were more modest at the RNA than protein level. Many proteins were present in multiple spots, suggesting that PTM is extensive in this organism. In several cases, different isoforms appeared to be specific to different life stages. Our results suggest that post-transcriptional controls at translational and post-translational levels could play major roles in differentiation in Leishmania parasites.
Subject(s)
Leishmania infantum/growth & development , Leishmania infantum/genetics , Life Cycle Stages , Proteomics/methods , Protozoan Proteins/metabolism , Transcription, Genetic , Animals , Electrophoresis, Gel, Two-Dimensional , Gene Expression Regulation, Developmental , Gene Targeting , Genes, Protozoan , Leishmania infantum/chemistry , Leishmania infantum/metabolism , Oligonucleotide Array Sequence Analysis , Peptide Fragments/chemistry , Peptide Mapping , Protein Isoforms/genetics , Protein Isoforms/metabolism , Proteome/analysis , Protozoan Proteins/genetics , RNA, Messenger/metabolismABSTRACT
We recently characterized a large developmentally regulated gene family in Leishmania encoding the amastin surface proteins. While studying the regulation of these genes, we identified a region of 770 nucleotides (nt) within the 2055-nt 3'-untranslated region (3'-UTR) that regulates stage-specific gene expression at the level of translation. An intriguing feature of this 3'-UTR regulatory region is the presence of a approximately 450-nt element that is highly conserved among several Leishmania mRNAs. Here we show, using a luciferase reporter system and polysome profiling experiments, that the 450-nt element stimulates translation initiation of the amastin mRNA in response to heat shock, which is the main environmental change that the parasite encounters upon its entry into the mammalian host. Deletional analyses depicted a second region of approximately 100 nucleotides located at the 3'-end of several amastin transcripts, which also activates translation in response to elevated temperature. Both 3'-UTR regulatory elements act in an additive manner to stimulate amastin mRNA translation. In addition, we show that acidic pH encountered in the phagolysosomes of macrophages, the location of parasitic differentiation, triggers the accumulation of amastin transcripts by a distinct mechanism that is independent of the 450-nt and 100-nt elements. Overall, these important findings support the notion that stage-specific post-transcriptional regulation of the amastin mRNAs in Leishmania is complex and involves the coordination of distinct mechanisms controlling mRNA stability and translation that are independently triggered by key environmental signals inducing differentiation of the parasite within macrophages.
Subject(s)
3' Untranslated Regions , Gene Expression Regulation, Developmental , Leishmania infantum/metabolism , Protein Biosynthesis , RNA, Messenger/metabolism , 5' Untranslated Regions , Animals , Blotting, Northern , Cell Differentiation , Centrifugation, Density Gradient , DNA/chemistry , Gene Deletion , Genes, Reporter , Genetic Vectors , Hydrogen-Ion Concentration , Luciferases/metabolism , Lysosomes/chemistry , Macrophages/cytology , Macrophages/metabolism , Models, Genetic , Open Reading Frames , Polyribosomes/metabolism , Protein Structure, Tertiary , RNA Processing, Post-Transcriptional , Signal Transduction , Sucrose/pharmacology , Transcription, Genetic , TransfectionABSTRACT
The ability of Leishmania amastigotes to survive within the drastic environmental changes encountered in the phagolysosomes of mammalian macrophages is heavily dependent on the developmental regulation of a variety of genes. The identification of genes that are expressed preferentially in the mammalian stage of the parasite should increase our understanding of the molecular mechanisms regulating stage-specific gene expression and of the determinants that control its intracellular survival and contribute to its pathogenesis. We report here detailed sequence characterization and structural organization of the amastin gene family in Leishmania major and Leishmania infantum and the study of their developmental gene regulation throughout the parasite's life cycle. Amastin surface proteins represent the largest developmentally regulated gene family reported so far in Leishmania comprising up to 45 members. All the members of the amastin gene family in both Leishmania and Trypanosoma species share a similar structural organization and contain a highly conserved 11 amino acid extracellular domain, which is unique to amastin proteins. The majority of the amastin gene homologs are specifically expressed in the amastigote stage of the parasite. Three distinct RNA elements were identified in the 3'-untranslated regions (3'UTR) of the amastin transcripts. The majority of these transcripts contain a conserved 450 nt cis-acting 3'UTR element shown previously to regulate stage-specific gene expression at the level of translation, which suggests that several amastin homologs may be regulated by a similar mechanism of translational control inside the macrophage. These findings further highlight the unique features of gene expression control in Leishmania.
Subject(s)
Gene Expression Regulation, Developmental , Genes, Protozoan , Leishmania infantum/genetics , Leishmania major/genetics , Membrane Glycoproteins/genetics , Membrane Proteins/genetics , Protozoan Proteins/genetics , 3' Untranslated Regions/genetics , Amino Acid Sequence , Animals , Conserved Sequence , Leishmania infantum/growth & development , Leishmania infantum/metabolism , Leishmania major/growth & development , Leishmania major/metabolism , Life Cycle Stages , Membrane Glycoproteins/biosynthesis , Membrane Glycoproteins/metabolism , Membrane Proteins/biosynthesis , Membrane Proteins/metabolism , Molecular Sequence Data , Phylogeny , Protozoan Proteins/biosynthesis , Protozoan Proteins/metabolism , Sequence Alignment , Sequence Homology, Amino AcidABSTRACT
Detailed analysis of the Leishmania donovani ribosomal RNA (rRNA) gene promoter region has allowed the identification of cis-acting sequences involved in plasmid DNA partitioning and stable plasmid inheritance. We report that plasmids bearing the 350 bp rRNA promoter along with the 200 bp region immediately 3' to the promoter exhibited a 6.5-fold increase in transformation frequency and were transmitted to daughter cells as single-copy molecules. This is in contrast to what has been observed for plasmid molecules in this organism so far. Moreover, we show that these low-copy-number plasmids displayed a remarkable mitotic stability in the absence of selective pressure. The region in the vicinity of the RNA pol I transcription initiation site, and also in the adjacent 200 nt, displays a complex structural organization and shares sequence similarity to the yeast autonomously replicating consensus sequence and centromere DNA elements. Deletion analyses indicated that these elements were necessary but not sufficient for plasmid DNA partitioning and stable inheritance, and that the rRNA promoter region was required for optimal function. These results suggest an interplay between RNA pol I transcription, DNA replication, DNA partitioning and mitotic stability in trypanosomatids. This is the first example of defined DNA elements for plasmid partitioning and stable inheritance in the protozoan parasite Leishmania.