The highly effective cadmium-resistant mechanism of Pseudomonas aeruginosa and the function of pyoverdine induced by cadmium.

Pyoverdine (PVD) plays an important role in reducing cadmium (Cd) accumulation in plants. Some Pseudomonas aeruginosa (P. aeruginosa) species can produce PVD under Cd(Π) stress. However, the function of Cd(Π)-induced PVD remains unclear. In this study, we isolated a highly effective Cd(Π)-resistant P. aeruginosa which can secrete PVD under Cd(Π) stress and found that PVD secretion has a dose-dependent relationship with Cd(Π) concentration. PVD can form a PVD-Cd complex with Cd(Π), though the PVD-Cd complex is unable to be adsorbed by the cell or enter the cell, so the complexation of PVD and Cd(Π) impedes Cd(Π) adsorption on the cell surface and alleviates the oxidative stress, lipid peroxidation, and morphological destruction of the cell caused by Cd(Π) and effectively improves the resistance of P. aeruginosa to Cd(Π). In summary, our research results indicate that the Cd(Π) resistance mechanism of P. aeruginosa screened is the complexation of PVD for Cd(Π) and the adsorption of bacteria for Cd(Π); furthermore, PVD plays an important role in improving the Cd(Π)-resistant ability of bacteria. This study provides a deeper understanding of the highly effective Cd(Π) resistance mechanism of P. aeruginosa and the function of Cd(Π)-induced PVD in bacteria.

Formation of Sb2O3 microcrystals by Rhodotorula mucilaginosa.

Antimony (Sb) pollution seriously endangers ecological environment and human health. Microbial induced mineralization can effectively convert metal ions into more stable and less soluble crystalline minerals by extracellular polymeric substance (EPS). In this study, an efficient Sb-resistant Rhodotorula mucilaginosa (R. mucilaginosa) was screened, which can resist 41 mM Sb(III) and directly transform Sb(III) into Sb2O3 microcrystals by EPS. The removal efficiency of R. mucilaginosa for 22 mM Sb(III) reached 70% by converting Sb(III) to Sb2O3. The components of supernatants as well as the effects of supernatants and pH on Sb(III) mineralization verified that inducible and non-inducible extracellular protein/polysaccharide biomacromolecules play important roles in the morphologies and sizes control of Sb2O3 formed by R. mucilaginosa respectively. Sb2O3 microcrystals with different morphologies and sizes can be prepared by the regulation of inducible and non-inducible extracellular biomacromolecules secreted by R. mucilaginosa. This is the first time to identify that R. mucilaginosa can remove Sb(III) by transforming Sb(III) into Sb2O3 microcrystals under the control of EPS. This study contributes to our understanding for Sb(III) biomineralization mechanisms and provides strategies for the remediation of Sb-contaminated environment.

Antimony(III) removal by biogenic manganese oxides formed by Pseudomonas aeruginosa PA-1: kinetics and mechanisms.

In this study, Pseudomonas aeruginosa PA-1, a manganese-oxidizing bacterium screened from the soil at a manganese mining area, was found to be tolerated to Sb(III) stress during the Mn(II) oxidation, and the generated biological manganese oxide (BMO) outperformed the identical type of Abiotic-MnOX in terms of oxidation and adsorption of Sb(III). Adsorption kinetics and isotherm experiments indicated that Sb(III) was primarily adsorbed through chemisorption and multilayer adsorption on BMO; the maximum adsorption capacity of BMO was 143.15 mg·g-1. Removal kinetic studies showed that the Sb(III) removal efficiency by BMO was 72.38-95.71% after 15 min, and it could be up to 96.32-98.31% after 480 min. The removal procedure could be divided into two stages, fast (within 15 min) and slow (15 ~ 480 min), both of which exhibited first-order kinetic behavior. Dynamic fitting in two steps revealed that the removal speed correlated to the level of dissolved Sb(III) with low Sb(III) concentrations, but with the initial concentration being high, the removal speed rate was independent of dissolved Sb(III). During the whole process, the Sb(III) removal speed by BMO was also higher than that by the Abiotic-MnOX. Combining multiple spectroscopic techniques revealed that Sb(V) was generated through the Sb(III) oxidation by BMO and replacing surface metal hydroxyl groups to form the complex internal Mn-O(H)-Sb(V) or generating stable Mn(II)-antimonate precipitates on the surface. In addition, microbial metabolites, including tryptophan and humus, in BMO may be complex with Sb(III) and Sb(V) to achieve the treatment of Sb(III). This research investigates the factors and mechanisms influencing the adsorption and removal of Sb(III) by BMO, which could aid in its future engineering applications for the BMO.