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1.
J Lasers Med Sci ; 14: e48, 2023.
Article in English | MEDLINE | ID: mdl-38028878

ABSTRACT

Introduction: Using lasers in melanin depigmentation is one of the main fields of interest for dental practitioners and patients. However, it is important to know what would happen inside the tissue and how the cells would interact inside the tissue with a laser. Methods: In this study, we used both wavelengths of 445 nm and 808 nm on sheep gingiva to find out the effects and side effects of these diode lasers while using them for gingival depigmentation. Results: After microscopic evaluation, we concluded that 808 nm and 445 nm lasers with a power of 1 W are safe enough to use in the depigmentation of gingiva, and both lasers are highly effective in melanin pigments which are located in basal membrane. Conclusion: The 445 nm blue laser produced a less thermal effect, which means it is safer to be used in gingival hyperpigmentation than a diode laser.

2.
J Orofac Orthop ; 2023 Jun 15.
Article in English | MEDLINE | ID: mdl-37318554

ABSTRACT

PURPOSE: To investigate the reusability of ceramic brackets in terms of shear bond strength, friction behavior, slot dimension, fracture strength, and color stability. METHODS: A total of 90 conventionally debonded and 30 by an Er:YAG laser debonded ceramic brackets were collected. All the used brackets were inspected under a stereomicroscope at 18 נmagnification and sorted according to their adhesive remnant index (ARI). Five groups were formed (n = 10): (1) new brackets as a control group, (2) flamed and sandblasted, (3) flamed and acid bathed, (4) laser-reconditioned, and (5) laser-debonded brackets. The bracket groups were tested regarding different properties such as shear bond strength, friction behavior, slot size, fracture strength, and color stability. Analysis of variance (ANOVA) and nonparametric Kruskal-Wallis tests were used for statistical analysis (significance level: p < 0.05). RESULTS: Shear bond strength values of the acid reconditioned brackets were significantly lower (8.0 ± 3.1 MPa) compared to the control group (12.9 ± 2.9 MPa). Laser-reconditioned (32.8 ± 2.7%) and laser-debonded (30.9 ± 2.4%) brackets showed the lowest force loss due to friction (control group 38.3 ± 3.0%). No significant differences were observed between groups regarding slot size and fracture strength. All groups had color differences of [Formula: see text]< 10. Scanning electron microscope images and ARI scores indicated that most of the residues on the bracket bases were removed. CONCLUSION: All reconditioning methods yielded adequate results regarding bracket properties. Yet, focusing on the need to protect the enamel and the bracket base, laser debonding seems to be the most suitable method for reconditioning ceramic brackets.

3.
Lasers Surg Med ; 55(3): 294-304, 2023 03.
Article in English | MEDLINE | ID: mdl-36740362

ABSTRACT

OBJECTIVES: To investigate quantitatively the cutting efficiency and the thermal effects in the surrounding soft tissues of incisions that are induced by a 940 nm-diode laser with different power settings. MATERIALS AND METHODS: Fifty-four gingival samples were prepared from the lower jaws of freshly slaughtered German-land race pigs and were randomly divided into 9 groups (n = 6) according to the adjusted output power (1, 1.5, 2, 2.5, 3, 3.5, 4, 5 and 6 W). Five incisions were implemented for each sample using a diode laser (940 nm) in continuous wave with an initiated tip resulting in 30 incisions for each experimental group utilizing a three-dimensional computer-controlled micropositioner. The samples were prepared for histometric evaluation using a transmitted light microscope. The cutting depth and width and the thermal damage were recorded for each sample and the efficiency factor γ was calculated. RESULTS: The highest cutting efficiency (γz = 0.81 ± 0.03) exhibited the group with 5 W output power (p < 0.05), while the lowest (γz = 0.45 ± 0.11) showed the 1-W group (p < 0.05). Over 3.5 W there was a rapid increase in the size of thermal damage of the incisions, especially for 6 W, which presented the largest. CONCLUSIONS: The most effective power parameters of diode laser (940 nm) for soft tissue surgery were from 3 to 5 W. The outcomes of the current study may help to establish clinical protocols for the use of diode lasers (940 nm) in soft tissue surgery in contact mode assisting dental professionals to achieve optimal clinical results and avoid complications.


Subject(s)
Laser Therapy , Animals , Gingiva , Laser Therapy/adverse effects , Laser Therapy/methods , Lasers, Semiconductor , Swine
4.
Materials (Basel) ; 16(3)2023 Jan 31.
Article in English | MEDLINE | ID: mdl-36770241

ABSTRACT

The use of dental ceramics as restorative materials requires corresponding luting materials (cements) that, in turn, influence the visual appearance of the restoration. Due to the high light transmission through the ceramics, the cements can affect the color perception of the dental restoration. This study aims to investigate the optical effects of various cements on the visual appearance of full-ceramic restorations. Three fixing polymer resins (Bifix SE (VOCO GmbH, Cuxhafen, Germany), BreezeTM (Pentron Clinical, West Collins Orange, CA, USA), and PanaviaTM F. 2.0 (Kuraray, Noritake, Osaka, Japan)), with layer thicknesses of 50, 100, 200, and 250 µm, were applied onto a ceramic base model (0.4 mm thick), and irradiated with laser light of wavelengths 532, 632.8, and 1064 nm. Light intensities and scattering effects of light of various wavelengths were angle-dependent, analyzed using a goniophotometer with perpendicular light incidence on the sample specimen (base model plus luting material). In addition, the transmitted power of the light through the sample specimen was determined as a function of the layer thickness. With increasing layer thickness, power losses of respectively 30% for Bifix SE and BreezeTM in the visible spectral range were comparable, whereas PanaviaTM F. 2.0 showed a power loss of ca. 44% here. For the near-infrared range, the power losses for all cements were 25%. This could be confirmed by the interpretation of the line widths. Moreover, the line widths for thin cement layer thicknesses (50 and 100 µm) in the visible spectral range displayed only a redistribution of light by scattering, which does not affect color perception at all. In addition, at 200 and 250 µm, absorption occurred which causes a change in color perception. Within the scope of this study, it could be shown that for thin-layer thicknesses of the cement applied here, there is no adverse optical effect on the aesthetic visual appearance of the restoration.

5.
J Lasers Med Sci ; 14: e63, 2023.
Article in English | MEDLINE | ID: mdl-38318220

ABSTRACT

Introduction: Nowadays, esthetic appearance plays an important role in the field of dentistry. Discolorations and pigmentations of the gingiva reduce the appearance of a healthy-looking smile. On this occasion, the use of lasers shows a promising approach for a fast and non-aggressive treatment in this field. Different laser wavelengths are being used for gingival depigmentation this clinical study aimed to investigate the effect of the novel laser wavelength (445 nm) in this field and compare it with an 810 nm diode laser. Methods: Two diode laser wavelengths (445 nm and 810 nm) were used for depigmentation. The laser output power chosen was 1 W. An optical fiber with a diameter of 400 µm was used. 21 patients with pigmented gingiva were selected. Depigmentation was carried out in a split-mouth design for a direct comparison of the clinical effect. Outcomes were documented by photograph after one month and six months of follow-up. Results: For each wavelength, 21 volunteers evaluated 21 clinical cases of depigmentation, which means that 441 comparisons were carried out in total regarding the color change from brownish to pinkish. A 100% clarification was achieved for 445 nm. In the 810 nm group, the color change in 44 of 441 cases (10%) could not be clearly identified. No statistically significant difference in pain experience was reported for both laser treatments. Conclusion: The clinical evaluation showed that within the limitations of this study, most of the clinical outcome parameters were highly acceptable by the patients due to mild pain and discomfort for both laser systems.

6.
Aust Endod J ; 48(1): 65-71, 2022 Apr.
Article in English | MEDLINE | ID: mdl-34843153

ABSTRACT

To measure the transmitted laser power in the coronal, middle and apical root thirds during vertical and horizontal irradiation of laser. 14 mm length whole roots and longitudinal root sections were irradiated using a 940 nm diode laser with 0.5 W in continuous mode for 5 s. A power detector was placed in front of the root apex for the vertical transmitted power measurement and placed laterally to root surface for the horizontal transmission experiment. The transmitted power from the root apex vertically was 53% of the irradiated power, while for the horizontal transmission experiment for the coronal, middle and apical root thirds were 25.6%, 40.4% and 41.3%, respectively. Irradiation of root canals with 940 nm laser power can be transmitted during vertical irradiation in more than 50% of the samples, whereas less transmission happens with horizontal irradiation at apical and middle root third than coronal.


Subject(s)
Disinfectants , Lasers, Semiconductor , Dental Pulp Cavity , Dentin , Lasers, Semiconductor/therapeutic use , Root Canal Preparation , Root Canal Therapy , Tooth Root
7.
Lasers Med Sci ; 36(8): 1633-1647, 2021 Oct.
Article in English | MEDLINE | ID: mdl-33496905

ABSTRACT

Within the scope of this ex vivo study, the cut efficiency was investigated with eight diode laser wavelengths in the range from 400 to 1500 nm. Incisions on porcine gingiva samples were generated in CW-mode at a power range of 0.5-4 W using a bare fiber (∅ = 320 µm) in contact and non-contact mode at a cut speed of 2 mm/s. Cut depths, cut widths, and thermal damages were recorded based on histological sections and were evaluated via measurement masks. Moreover, with respect to the controllability of a therapeutic measure, an efficiency factor was defined. At powers above 2 W, for 445 nm, the maximum cut depth was 820 µm and 344 µm for 810 nm, respectively. At all wavelength and power ranges, the cut width averaged 125 µm. At minimum output power (0.5 W), the spatial expansion of the thermal damage in the tissue surface layer corresponds in the blue/green wavelength range from the very beginning of the laser impact to the fiber core diameter. It could be shown that increases in the diode laser power output do not correlate to the same extent with the incision depth nor with thermal damage to tissue.


Subject(s)
Laser Therapy , Lasers, Semiconductor , Animals , Gingiva/surgery , Lasers, Semiconductor/therapeutic use , Swine
8.
Sci Rep ; 10(1): 21710, 2020 12 10.
Article in English | MEDLINE | ID: mdl-33303956

ABSTRACT

To examine the applicability of plasma-mediated vitreous body removal, a diode-pumped Q-switched Neodymium:YAG laser was used for a possible application in eye surgery/vitrectomy. On a total of 1500 porcine vitreous bodies, removal rates were evaluated by comparing different LaserVit-tip designs (Mark I/II Gauge 19 and Mark III Gauge 22). The Nd:YAG laser, operating at a wavelength of 1064 nm and a pulse duration of 4 ns, was utilized for vitreous body removal with respective settings of 2, 3 and 4 mJ and pulse repetition rates (cut rates) from 5 to 25 Hz (300-1500 /min) in 5 Hz-steps as well as for 100 Hz (6000 cuts/min). The exposure times were selected at 10, 20, 40 and 60 s, respectively. Comparative measurements were carried out with mechanical cutters (Gauge 20 and Gauge 23), applying a fixed cut rate of 800 /min (13.33 Hz) at identical exposure times. The LaserVit-tips showed successful vitreous body removal for all laser settings and exposure times (Mark I: 6.2 g/min, Mark II: 8.2 g/min at 1500 cuts/min and 3 mJ, Mark II: 10.1 g/min, Mark III: 3.6 g/min at 6000 cuts/min at 3 mJ). Similar tip-dimensions (Gauge 22laser and Gauge 23cutter) showed comparable removal rates of 3.6 g/minlaser and 1.3 g/mincutter with settings of 6000 cuts/min at 3 mJ (laser) and 800 cuts/min for the mechanical cutter. A diode-pumped Q-switched Nd:YAG laser can successfully and gently remove vitreous body. The efficiency of the laser was comparable to that of mechanical cutters in terms of quantity of material removed per time unit.

9.
PLoS One ; 15(8): e0237195, 2020.
Article in English | MEDLINE | ID: mdl-32764819

ABSTRACT

This study investigated the caries-preventive effect of 445 nm laser radiation in combination with fluoride on the prevention of white spot lesions. Previously, several studies have indicated the ability of 488 nm argon ion laser irradiation to reduce early enamel demineralization. A diode laser (445 nm) could be an alternative technology for possible caries-preventive potential. Each sample of a group of seventeen caries-free bovine teeth was treated in four different ways on four different zones of the labial surface: control/no treatment (C), laser irradiation only (L) (0.3 W, 60 s and applied dose of 90 J/cm2), amine fluoride application only (10,000 ppm and pH 3.9) (F), and amine fluoride application followed by laser irradiation (FL). After treatment, the teeth were subjected to a demineralization solution (pH 4.3 for 48 h at 37 °C) to induce subsurface lesions. After sectioning, the teeth were examined by light microscopy. Three teeth were analyzed by scanning electron microscopy (SEM). The depths of the subsurface lesions in the C, L, F, and FL groups were 103.01 (± 13.04), 96.99 (± 14.51), 42.59 (± 17.13), and 24.35 (± 11.38) µm, respectively. The pairwise group comparison showed the following results: p < 0.001 for FL versus C, FL versus L, F versus C, and F versus L, p = 0.019 for FL versus F and p = 0.930 for L versus C. The SEM micrographs support the light-microscopic examination. The results of the current study have shown that using relatively low irradiation settings of 445 nm laser on fluoridated enamel may be effective for prevention of white spot lesions.


Subject(s)
Dental Caries/prevention & control , Fluorides, Topical/administration & dosage , Low-Level Light Therapy/methods , Tooth Demineralization/prevention & control , Animals , Cattle , Combined Modality Therapy/methods , Dental Enamel/drug effects , Dental Enamel/radiation effects , Dental Enamel/ultrastructure , Humans , Lasers, Semiconductor/therapeutic use , Microscopy, Electron, Scanning
10.
J Lasers Med Sci ; 11(4): 417-426, 2020.
Article in English | MEDLINE | ID: mdl-33425292

ABSTRACT

Introduction: Cavity disinfection is necessary to prevent a progressive infection of the crown dentin and pulp. Increasing intolerance and resistance to antiseptics and antibiotics as well as the controversy over the effects of those on the dental hard tissue and composite have prompted the investigation of alternative treatment options. The objective of this pilot study is to evaluate the antibacterial potential of a diode laser with a wavelength of 445 nm in the cavity preparation using the bacterium Streptococcus salivarius associated with caries in conjunction with the characteristics and influences of dentin on light transmission. Methods: The bactericidal effect of the laser irradiation was determined in culture experiments by using caries-free human dentin samples on bacteria-inoculated agar. For this, dentin discs (horizontally cut coronal dentin) of 500 µm and 1000 µm thicknesses were produced and irradiated with the laser with irradiation parameters of 0.7-1 W in a cw-mode and exposure times of between 5-30 s. Based on the different sample thicknesses, the penetration depth effect of the irradiation was ascertained after the subsequent incubation of the bacteria-inoculated agar. Additional influential parameters on the irradiation transmission were investigated, including surface moisture, tooth color as well as the presence of a smear layer on the dentin surface. Results: The optical transmission values of the laser radiation for dentin were significantly dependent on the sample thickness (P = 0.006) as well as its moisture content (P = 0.013) and were independent of the presence of a smear layer. There was a 40% reduction in bacteria after the radiography of the 500-µm-thick dentin samples, which was shown as the lowest laser dose (443 J/cm2). Conclusion: These findings indicate that the diode laser with light emission at a wavelength of 445 nm is interesting for the supportive cavity disinfection within the scope of caries therapy and show potential for clinical applications.

11.
Lasers Med Sci ; 35(1): 13-30, 2020 Feb.
Article in English | MEDLINE | ID: mdl-31399861

ABSTRACT

Since the invention of lasers in dentistry, investigations in caries prevention by the use of laser radiation have been proposed. There are several mechanisms stated for this purpose such as photothermal and/or photochemical interaction processes with the enamel. Alone or in conjugation with topical fluoride application, this treatment modality may improve enamel acid resistance in high-caries-risk populations. Data collection was done by searching the keywords caries, prevention, and laser in PubMed, Embase, Web of Science, Cochrane Library, and Google Scholar. Lasing protocols of the collected literature and their effectiveness as well as examination methods used to verify treatment outcomes have been evaluated. One hundred eighteen publications were found for the last 10 years. The wavelengths investigated for caries prevention are mainly located in the near and the mid-infrared spectral range. In the evaluated period of time, investigations using CO2; Er:YAG; Er,Cr:YSGG; Er:YLF; fundamental, second, and third harmonic generations of Nd:YAG; diodes; and argon ion lasers were found in the databases. Accounting for 39% of the literature, CO2 laser was the most examined system for this purpose. Reviewing the literature in this narrative review showed that all laser systems presented a positive effect in varying degrees. Laser irradiation could be an alternative or synergistic to topical fluoridation for enamel caries prevention with longer lasting effect. Further research should be focused on selecting proper laser settings to avoid damage to enamel and developing effective evidence-based clinical protocols.


Subject(s)
Dental Caries/prevention & control , Dental Caries/surgery , Dental Enamel/pathology , Dental Enamel/radiation effects , Humans , Lasers, Gas/therapeutic use , Risk , Treatment Outcome
12.
Biomed Res Int ; 2019: 9101642, 2019.
Article in English | MEDLINE | ID: mdl-31781651

ABSTRACT

OBJECTIVE: This in vitro study is aimed at investigating the caries preventive effectiveness of 445 nm diode laser in combination with topical fluoridation. MATERIALS AND METHODS: A total of 30 caries-free bovine teeth were used in this study. Eighteen teeth were covered with nail varnish except four windows on the labial surface. The windows were assigned to no treatment/control (C), laser (L) (0.3 W, 60 s, and 90 J/cm2), fluoride (F), and fluoride followed by laser (FL) treatment groups. Artificial caries lesions were created, and the teeth were sectioned and investigated under polarized light microscopy for quantitative measurement of the resulted lesion depth. Ten teeth were used for surface temperature measurement and two teeth for scanning electron microscopy (SEM). Extra twelve human molars were used for the intrapulpal temperature measurement. The absorbance of fluoride at 445 nm was measured. RESULTS: The means of lesion depth for the C, L, F, and FL groups were 123.48 (±21.93), 112.33 (±20.42), 99.58 (±30.68), and 89.03 (±30.38) µm, respectively. The pairwise differences of the L, F, and FL groups compared with the C group were significant (p < 0.05). The differences between groups were tested: FL versus L p=0.02, F versus L p=0.16, and FL versus F p=0.91, and the difference of the F versus FL was not significant (p=0.91). Temperature increment at the enamel surface and pulp roof were ∆T = 16.67 (±4.11) and 2.12 (±0.66)°C, respectively. The topical fluoride absorbance at 445 nm is five orders higher than that at 810 nm. SEM shows that after laser irradiation the enamel surface was intact and without thermal damage. CONCLUSIONS: The 445 nm laser irradiation may be useful for caries prevention, and its effectiveness is lower than those previously achieved using the argon ion laser.


Subject(s)
Dental Caries/prevention & control , Fluorides, Topical/therapeutic use , Lasers, Semiconductor/therapeutic use , Low-Level Light Therapy , Animals , Cattle , Dental Caries/drug therapy , Dental Caries/radiotherapy , Dental Caries Susceptibility , Dental Enamel/ultrastructure , Dental Pulp/ultrastructure , Humans , Microscopy, Electron, Scanning , Microscopy, Polarization , Molar/ultrastructure , Temperature , Tooth/ultrastructure
13.
Photodiagnosis Photodyn Ther ; 26: 29-35, 2019 Jun.
Article in English | MEDLINE | ID: mdl-30807835

ABSTRACT

BACKGROUND: The objective was to evaluate the efficiency of indocyanine green (ICG)-based adjunctive antimicrobial photodynamic therapy (aPDT) in a prospective clinical study regarding non-surgical treatment of chronic periodontitis. METHODS: Affected teeth of twenty patients were treated with scaling and root planing (control group). Using a split-mouth design, two quadrants received additional ICG-based (perio green®, 0.1 mg/ml) aPDT (test group) with a diode laser at 808 nm (100 mW at 2 kHz). Clinical assessment of bleeding on probing (BOP), sulcus fluid flow rate (SFFR) and microbiological analysis were performed at baseline, two weeks, three and six months after treatment. Relative attachment level (RAL), probing depths (PD) and gingival recession (GR), were also analyzed. RESULTS: At baseline, none of the assessed parameters showed significant differences between the test and control groups. Median values for BOP, RAL, PD, decreased significantly in both groups after three months of treatment (p ≤ 0.05) without significant difference between the groups. Two weeks after treatment, the SFFR showed significantly lower mean values in the test group (aPDT). CONCLUSION: Within the study limits, the only significant difference between the control group and the aPDT group was a transient smaller amount of SFFR in the latter during the first follow-up. With the applied parameters, this study does not conclusively support ICG-based aPDT, though it is promising because no adverse effects occurred. The precise modes of action of ICG must be elucidated, and further clinical trials are needed.


Subject(s)
Chronic Periodontitis/drug therapy , Indocyanine Green/therapeutic use , Photochemotherapy/methods , Photosensitizing Agents/therapeutic use , Dental Scaling , Female , Humans , Male , Middle Aged , Periodontal Index , Root Planing
14.
Lasers Med Sci ; 34(4): 729-736, 2019 Jun.
Article in English | MEDLINE | ID: mdl-30291465

ABSTRACT

Challenges especially in the minimal invasive restorative treatment of teeth require further developments of composite polymerization techniques. These include, among others, the securing of a complete polymerization with moderate thermal stress for the pulp. The aim of this study is to compare current light curing sources with a blue diode laser regarding curing depth and heat generation during the polymerization process. A diode laser (445 nm), a LED, and a halogen lamp were used for polymerizing composite resins. The curing depth was determined according to the norm ISO 4049. Laser output powers of 0.1, 0.5, 1, and 2 W were chosen. The laser beam diameter was adapted to the glass rod of the LED and the halogen lamp (8 mm). The irradiation time was fixed at 40 s. To ascertain ΔT values, the surface and ground area temperatures of the cavities were simultaneously determined during the curing via a thermography camera and a thermocouple. The curing depths for the LED (3.3 mm), halogen lamp (3.1 mm) and laser(0.5/1 W) (3/3.3 mm) showed no significant differences (p < 0.05). The values of ΔTsurface as well as ΔTground also showed no significant differences among LED, halogen lamp, and laser(1 W). The ΔTsurface values were 4.1LED, 4.3halogen lamp, and 4.5 °C for the laser while the ΔTground values were 2.7LED, 2.6halogen lamp, and 2.9 °C for the laser. The results indicate that the blue diode laser (445 nm) is a feasible alternative for photopolymerization of complex composite resin restorations in dentistry by the use of selected laser parameters.


Subject(s)
Composite Resins/radiation effects , Halogens/chemistry , Lasers, Semiconductor , Light , Polymerization/radiation effects , Curing Lights, Dental , Materials Testing , Polymers/chemistry , Temperature
15.
Clin Oral Investig ; 21(4): 985-994, 2017 May.
Article in English | MEDLINE | ID: mdl-27251194

ABSTRACT

OBJECTIVES: The objective of this study was to examine a new blue light diode laser system (445 nm) for dental soft tissue surgery on cellular level. MATERIALS AND METHODS: An in vitro cell culture model was established to evaluate the effects of the 445-nm diode laser in comparison to an established infrared diode laser (IR). Monolayer cell cultures were irradiated and wound healing was morphometrically measured. Fluorescence staining was used for proof of potential DNA double-strand breaks as well as cytoskeleton alterations. Cellular live/dead discrimination was performed and temperature development during laser irradiation was measured with a thermographic infrared camera. RESULTS: A characteristic zone formation was detected after irradiation with both wavelengths. Despite a larger wound area after irradiation with 445 nm, due to its higher temperature development, this laser system showed a faster wound healing in comparison to the IR laser. No increase of devitalized cells was documented with higher distances between laser tip and cell layer and thus without thermal interaction. Neither cytoskeleton alteration nor DNA double-strand breaks could be recorded after irradiation in non-contact mode. CONCLUSIONS: The blue diode laser system demonstrated an excellent direct thermal coupling to cells and tissues without side effects even by reduced power settings. CLINICAL RELEVANCE: The blue diode laser seems to be a promising technology for clinical application due to high absorption of blue light without major side effects in adjacent tissues even by reduced power settings.


Subject(s)
Cells, Cultured/radiation effects , Lasers, Semiconductor , Wound Healing/radiation effects , Cytoskeleton/radiation effects , DNA Damage/radiation effects , Dentistry , In Vitro Techniques , Staining and Labeling , Thermography
16.
Microsc Microanal ; 22(6): 1189-1197, 2016 12.
Article in English | MEDLINE | ID: mdl-27938451

ABSTRACT

The aim of this study was to compare surface structures of laser-irradiated dental hard tissues using confocal (CFM), atomic force (AFM), and scanning electron microscopy (SEM). The general potential of the AFM in analyzing laser-irradiated surfaces was determined in this context. Specimens of human enamel and dentin were irradiated using an 8.6 W Nd:YVO4 laser with a pulse duration of 8 ps, λ Center=1,064 nm, and a pulse repetition rate of 500 kHz. Surface topology of irradiated areas (1 mm2) was investigated using AFM, CFM, and SEM. Surface roughness R z was measured only with the AFM and the CFM. For non-irradiated enamel and dentin surfaces, roughnesses for CFM and AFM are in the nanometer range. However, major differences in roughness were determined for laser-prepared surfaces. For enamel, R z (CFM)=2.33 µm is much higher compared with R z (AFM)=0.09 µm; in the case of dentin, R z (CFM)=5.35 µm is also much higher compared with R z (AFM)=0.093 µm. Information regarding structural properties of surfaces needs real dimensions, particularly for use in dentistry. In this respect, AFM technology provides no additional results that lead to a significant improvement.


Subject(s)
Dental Enamel/radiation effects , Dental Enamel/ultrastructure , Dentin/radiation effects , Dentin/ultrastructure , Lasers , Humans , Microscopy, Atomic Force , Microscopy, Confocal , Microscopy, Electron, Scanning , Surface Properties
18.
Lasers Med Sci ; 30(3): 977-83, 2015 Apr.
Article in English | MEDLINE | ID: mdl-24458532

ABSTRACT

Ultrashort pulsed lasers (USPLs) represent a new generation of laser systems in the field of biophotonical applications. In terms of a pilot project, the study was carried out to evaluate the ablation parameters of bone tissue regarding the medical use of such a laser technology in dentistry. Specimens from ribs of freshly slaughtered pigs were assembled and irradiated with an USP Nd:YVO4 laser (pulse duration 8 ps at 1,064 nm with repetition rates between 50 and 500 kHz) using eligible average output powers in the range of 3.5-9 W and fluences between 1 and 2.5 J/cm(2). Square-shaped cavities of 1-mm edge length in the bone compacta were created employing a scanner system. Cavities were analyzed with an optical profilometer to determine the ablated volume. Ablation rate was calculated by the ablated volume and the recorded irradiation time by the scanner software. Additionally, samples were examined histologically to investigate side effects of the surrounding tissue. Formed cavities showed a precise and sharp-edged appearance in bone compacta. Optimized ablation rate of 5.2 mm(3)/min without any accompanying side effects was obtained with an average output power of 9 W, a pulse repetition rate of 500 kHz, and an applied fluence of 2.5 J/cm(2). Provided that the used laser system will be advanced and adjusted for clinical applications, the outcome of this study shows auspicious possibilities for the use of USPL systems in the preparation of bone tissue.


Subject(s)
Bone and Bones/radiation effects , Laser Therapy/instrumentation , Lasers , Oral Surgical Procedures/instrumentation , Animals , Pilot Projects , Sus scrofa
19.
Lasers Med Sci ; 30(5): 1427-34, 2015 Jul.
Article in English | MEDLINE | ID: mdl-24865391

ABSTRACT

The aim of the study was to investigate the efficiency of caries removal employing an ultrashort pulsed laser (USPL) and to compare the results regarding to the ablation rate of sound enamel and dentin including surface texture. The study was performed with 59 freshly extracted carious human teeth. Two cavities with an edge length of 1 × 1 mm per tooth were created: one in the dental decay and one in sound hard tissue. For this purpose a 9-W Nd:YVO4 laser with a center wavelength of 1,064 nm and a pulse duration of 8 ps at a repetition rate of 500 kHz was used. A scanner system moved the laser beam across the surface with a scan speed of 2,000 mm/s. Ablated volume and roughness R z of the cavity ground were measured using an optical profilometer. Subsequently, the specimens were cut to undecalcified sections for histological investigations. The removal of dental decay (dentin, 14.9 mm(3)/min; enamel, 12.8 mm(3)/min) was significantly higher (p < 0.05) compared to the removal of sound tissues (dentin, 4.2 mm(3)/min; enamel, 3.8 mm(3)/min). The arithmetic means of the surface roughness R z were 8.5 µm in carious enamel, 15.43 µm in carious dentin, 4.83 µm in sound enamel and 5.52 µm in sound dentin. Light microscopic investigations did not indicate any side effects in the surrounding tissues. Regarding the ablation rate of dental decay using the USPL system, caries removal seems to be much more efficient for cavity preparation.


Subject(s)
Dental Caries/surgery , Dental Cavity Preparation/methods , Lasers, Solid-State , Dental Enamel/pathology , Dentin/pathology , Humans , Laser Therapy , Surface Properties , Tooth/pathology
20.
Lasers Med Sci ; 29(6): 1775-83, 2014 Nov.
Article in English | MEDLINE | ID: mdl-23609558

ABSTRACT

Modern ultrashort pulse lasers with scanning systems provide a huge set of parameters affecting the suitability for dental applications. The present study investigates thresholds and ablation rates of oral hard tissues and restorative materials with a view towards a clinical application system. The functional system consists of a 10 W Nd:YVO4 laser emitting pulses with a duration of 8 ps at 1,064 nm. Measurements were performed on dentin, enamel, ceramic, composite, and mammoth ivory at a repetition rate of 500 kHz. By employing a scanning system, square-shaped cavities with an edge length of 1 mm were created. Ablation threshold and rate measurements were assessed by variation of the applied fluence. Examinations were carried out employing a scanning electron microscope and optical profilometer. Irradiation time was recorded by the scanner software in order to calculate the overall ablated volume per time. First high power ablation rate measurements were performed employing a laser source with up to 50 W. Threshold values in the range of 0.45 J/cm(2) (composite) to 1.54 J/cm(2) (enamel) were observed. Differences between any two materials are statistically significant (p < 0.05). Preparation speeds up to 37.53 mm(3)/min (composite) were achieved with the 10 W laser source and differed statistically significant for any two materials (p < 0.05) with the exception of dentin and mammoth ivory (p > 0.05). By employing the 50 W laser source, increased rates up to ∼50 mm(3)/min for dentin were obtained. The results indicate that modern USPL systems provide sufficient ablation rates to be seen as a promising technology for dental applications.


Subject(s)
Dental Enamel/radiation effects , Dental Materials/chemistry , Dentin/radiation effects , Laser Therapy/methods , Lasers , Tooth/radiation effects , Dentistry , Humans , Light , Software
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